FastQCFastQC Report
Thu 26 May 2016
SRR937154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479696
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.5655891480412192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67360.45522864155880666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47270.31945751019128255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29190.1972702501054271No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.11549669661876494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.032.0306321
GTATCAA95900.031.2212831
ATCAACG116650.025.5315233
TCAACGC117150.025.4225524
TATCAAC117650.025.3952582
CAACGCA118900.025.0483765
AACGCAG121600.024.6077296
GTACATG60600.023.4491651
GAGTACT74350.023.00245512-13
TACATGG62400.022.4559462
GTACTTT75450.022.3792214-15
ACGCAGA134400.022.1626117
GTATAGG3650.022.1352731
CGCAGAG135250.021.9882018
GTACGGG1302.9433917E-421.9216126
AGTACTT75500.021.80263312-13
AGAGTAC109950.021.5172510-11
ACATGGG63150.021.3618533
GCAGAGT141750.020.5777539
CAATCGG600.004767083619.79034274-75
Produced by FastQC (version 0.11.5)