##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1479696 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.220456093684106 33.0 31.0 34.0 31.0 34.0 2 32.46147924979185 34.0 31.0 34.0 31.0 34.0 3 32.53559379764492 34.0 31.0 34.0 31.0 34.0 4 36.142623214498116 37.0 35.0 37.0 35.0 37.0 5 36.001644256658125 37.0 35.0 37.0 35.0 37.0 6 36.09616029238438 37.0 35.0 37.0 35.0 37.0 7 36.05837550415761 37.0 35.0 37.0 35.0 37.0 8 36.035558655291354 37.0 35.0 37.0 35.0 37.0 9 37.7455693601929 39.0 37.0 39.0 35.0 39.0 10-11 37.67071378174977 39.0 37.0 39.0 35.0 39.0 12-13 37.582533844789744 39.0 37.0 39.0 35.0 39.0 14-15 38.81231651636553 40.0 38.0 41.0 35.0 41.0 16-17 38.797096160292384 40.0 38.0 41.0 35.0 41.0 18-19 38.8003106719218 40.0 38.0 41.0 35.0 41.0 20-21 38.720363845006 40.0 38.0 41.0 34.5 41.0 22-23 38.58832962986992 40.0 38.0 41.0 34.0 41.0 24-25 38.445135690033624 40.0 38.0 41.0 34.0 41.0 26-27 38.2376616548264 40.0 38.0 41.0 34.0 41.0 28-29 38.22079095976471 40.0 38.0 41.0 34.0 41.0 30-31 38.34015973551324 40.0 38.0 41.0 34.0 41.0 32-33 38.29936047674657 40.0 38.0 41.0 34.0 41.0 34-35 38.18045395811031 40.0 38.0 41.0 34.0 41.0 36-37 38.02236844595106 40.0 37.5 41.0 33.5 41.0 38-39 37.88860684897438 40.0 37.0 41.0 33.0 41.0 40-41 37.728642572528415 40.0 37.0 41.0 33.0 41.0 42-43 37.52629763140537 40.0 37.0 41.0 33.0 41.0 44-45 37.242158186546426 40.0 37.0 41.0 32.5 41.0 46-47 36.89839568397833 40.0 36.5 41.0 31.0 41.0 48-49 36.576192677414824 39.5 36.0 41.0 31.0 41.0 50-51 36.35976578972978 39.0 36.0 41.0 30.0 41.0 52-53 36.13337570690196 39.0 35.5 41.0 30.0 41.0 54-55 35.928516735870076 39.0 35.0 40.0 29.0 41.0 56-57 35.68463184329754 39.0 35.0 40.0 28.5 41.0 58-59 35.42999710751398 38.0 35.0 40.0 28.0 41.0 60-61 35.09220441225766 38.0 34.5 40.0 28.0 41.0 62-63 34.73733084363275 38.0 34.0 40.0 26.5 41.0 64-65 34.39323516452028 37.0 34.0 40.0 26.0 41.0 66-67 33.99340742963419 36.5 33.5 39.0 26.0 41.0 68-69 33.55517417091078 36.0 33.0 39.0 26.0 40.0 70-71 33.166671397368106 35.5 33.0 38.5 25.0 40.0 72-73 32.72744435343476 35.0 32.0 38.0 24.0 40.0 74-75 32.281843027216404 35.0 32.0 37.0 23.0 39.0 76-77 30.981567497648165 34.0 30.5 36.0 21.5 38.5 78-79 31.60352734615759 35.0 31.5 36.0 22.0 39.0 80-81 31.415117699851862 35.0 31.5 36.0 21.5 37.5 82-83 31.081728611822967 34.0 31.0 35.5 20.0 37.0 84-85 30.749811447756834 34.0 31.0 35.0 19.5 37.0 86-87 30.41852177744618 34.0 31.0 35.0 18.0 36.0 88-89 30.05944396686887 34.0 30.5 35.0 15.0 36.0 90-91 29.616900025410626 34.0 30.0 35.0 7.0 35.5 92-93 29.207220266865626 34.0 29.5 35.0 2.0 35.0 94-95 28.804792335723015 34.0 29.0 35.0 2.0 35.0 96-97 28.376210721661746 34.0 29.0 35.0 2.0 35.0 98-99 27.740138514938202 33.5 28.5 35.0 2.0 35.0 100-101 26.141978487473104 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.038366312618826726 1101 2 0.056208534279875266 1101 3 0.0706700393065347 1101 4 0.012003860082103301 1101 5 -0.15294966024697487 1101 6 -0.0796767707042676 1101 7 -0.056457698932220524 1101 8 -0.03240134350179602 1101 9 0.046027388542576375 1101 10-11 -0.055740346732576995 1101 12-13 0.008461283969680267 1101 14-15 0.1335300592832951 1101 16-17 0.027389497287110487 1101 18-19 0.13352259201717942 1101 20-21 0.07438512615242132 1101 22-23 0.01704604246135233 1101 24-25 0.018350134266611917 1101 26-27 -0.02695271522098608 1101 28-29 -0.12882171532666575 1101 30-31 -0.0018204925409648354 1101 32-33 -0.07025581099273381 1101 34-35 -0.009673803779946866 1101 36-37 0.06905517215667345 1101 38-39 -0.016977134883795486 1101 40-41 -0.1364318540163154 1101 42-43 -0.17159048657782705 1101 44-45 -0.21626642951882502 1101 46-47 -0.11041497715328319 1101 48-49 -0.04668161109101021 1101 50-51 -0.17455805603912466 1101 52-53 -0.19784986616074463 1101 54-55 -0.20539503609384013 1101 56-57 -0.10348953726747823 1101 58-59 -0.22985905530842388 1101 60-61 -0.2597147774832038 1101 62-63 -0.24658528765810672 1101 64-65 -0.11412836761275003 1101 66-67 -0.23823619268279828 1101 68-69 -0.39508435607580594 1101 70-71 -0.43677928087027595 1101 72-73 -0.48025197116923124 1101 74-75 -0.34028234087042364 1101 76-77 -0.36083239126856625 1101 78-79 -0.3501059133966109 1101 80-81 -0.5054294687493979 1101 82-83 -0.5040710840399605 1101 84-85 -0.43115170610380815 1101 86-87 -0.49568942454144604 1101 88-89 -0.37901988614174087 1101 90-91 -0.3483788790344384 1101 92-93 -0.3513342643816557 1101 94-95 -0.5524463488932589 1101 96-97 -0.4476994836512098 1101 98-99 -0.6875128421149235 1101 100-101 -0.6183695460596645 1102 1 0.12408625610105162 1102 2 0.09407986898227705 1102 3 0.032817807891184714 1102 4 -7.221906278118695E-4 1102 5 -0.058285883923169024 1102 6 -0.018311421504328962 1102 7 0.09096958918145504 1102 8 0.04360551038484317 1102 9 0.08927862821776955 1102 10-11 0.09848526541711777 1102 12-13 0.0646789962112777 1102 14-15 0.06755452779839999 1102 16-17 0.06463135538462694 1102 18-19 0.04854371629221532 1102 20-21 0.12073389393216871 1102 22-23 0.06833300994266978 1102 24-25 0.10649770948871407 1102 26-27 0.12691238062743082 1102 28-29 0.042569947486335025 1102 30-31 -0.04287951354410069 1102 32-33 -0.08281808596125018 1102 34-35 0.04804095385874518 1102 36-37 0.04449696479049692 1102 38-39 0.06261414796017561 1102 40-41 0.17431211417904535 1102 42-43 0.25095709982668524 1102 44-45 0.18815889351892423 1102 46-47 0.19825092615845108 1102 48-49 0.2819097195111695 1102 50-51 0.13461687998233884 1102 52-53 0.24913583316516252 1102 54-55 0.09439704093433932 1102 56-57 0.10017794439511363 1102 58-59 -0.10292377002295439 1102 60-61 -0.11661694652166688 1102 62-63 -0.10416935085736867 1102 64-65 0.03829326522905774 1102 66-67 0.05111570500745444 1102 68-69 -0.06038253625474965 1102 70-71 -0.120534148422756 1102 72-73 -0.038847809681222145 1102 74-75 -0.15154699012126116 1102 76-77 -0.36027641572543345 1102 78-79 -0.19129120246778442 1102 80-81 0.03027236155516988 1102 82-83 -0.03487874538993552 1102 84-85 -0.14106689449833 1102 86-87 -0.08533369571027549 1102 88-89 0.04713787206422637 1102 90-91 0.022980750827727547 1102 92-93 0.21980160075845845 1102 94-95 -0.044205144267934315 1102 96-97 -0.004172877082240234 1102 98-99 0.2060820820019309 1102 100-101 0.12610057651588136 1103 1 0.08610865518787136 1103 2 0.0540875110805672 1103 3 -0.004973819174423966 1103 4 0.05422546341730339 1103 5 -0.03276949815197838 1103 6 0.016330983918656727 1103 7 -0.027923080450150906 1103 8 -0.01845824309460653 1103 9 -0.06399792142321559 1103 10-11 -0.004849809153803619 1103 12-13 0.06809532853613831 1103 14-15 0.07756386276656002 1103 16-17 0.03850142520341393 1103 18-19 -0.08947076500037099 1103 20-21 -0.1585497319048983 1103 22-23 -0.09011864290292237 1103 24-25 -0.04925200211880565 1103 26-27 -0.047171353553309814 1103 28-29 -0.02186684679853812 1103 30-31 -0.10062584424864696 1103 32-33 0.05538877790056773 1103 34-35 0.015027210173755634 1103 36-37 0.06507984407194556 1103 38-39 0.13649981636566366 1103 40-41 0.08574273432564183 1103 42-43 0.08909709532848353 1103 44-45 0.023524879893741968 1103 46-47 0.07560891251392832 1103 48-49 -0.026989576496795564 1103 50-51 0.0782694643900399 1103 52-53 0.29017631935739274 1103 54-55 0.2493963275106097 1103 56-57 0.172722134344788 1103 58-59 0.11656055762958317 1103 60-61 0.27586513928748957 1103 62-63 0.17908901252440756 1103 64-65 0.18768201828529385 1103 66-67 0.11060980293756018 1103 68-69 0.23626086554308756 1103 70-71 0.26818408306026953 1103 72-73 0.3867499547631965 1103 74-75 0.3332917882071058 1103 76-77 0.09651682924009108 1103 78-79 0.2866101951172162 1103 80-81 0.28606125100834845 1103 82-83 0.17633675936340154 1103 84-85 0.16818343016992898 1103 86-87 0.23855027982533983 1103 88-89 0.37534809748193965 1103 90-91 0.42865379961381933 1103 92-93 0.31624742045814 1103 94-95 0.19040727317443285 1103 96-97 0.3171915034845796 1103 98-99 0.39275169909894814 1103 100-101 0.5984261444132599 1104 1 -0.06484567278581466 1104 2 -0.04595159365111101 1104 3 0.03068768336738259 1104 4 -0.04217776387319816 1104 5 -0.19628909652337967 1104 6 -0.14606866643903516 1104 7 -0.12461815555423783 1104 8 -0.05874186741314702 1104 9 -0.06854959717598774 1104 10-11 -0.028454259042234753 1104 12-13 -0.087605480011689 1104 14-15 -0.07135975074066891 1104 16-17 -0.11596226490308226 1104 18-19 -0.0997386324123255 1104 20-21 -0.18882714547194013 1104 22-23 -0.2634963716080989 1104 24-25 -0.2212093089529361 1104 26-27 -0.2560934143726712 1104 28-29 -0.3043496522179936 1104 30-31 -0.17590532806619308 1104 32-33 -0.32753352444801465 1104 34-35 -0.13869392133315728 1104 36-37 -0.09722348213053067 1104 38-39 -0.11774563483393052 1104 40-41 -0.110789452318933 1104 42-43 0.018532463258011944 1104 44-45 -0.04344269193245509 1104 46-47 0.02318269394147876 1104 48-49 -0.11256927539036354 1104 50-51 -0.08602360257106767 1104 52-53 0.0011434713827966902 1104 54-55 0.020906086441435434 1104 56-57 -0.05261744424421977 1104 58-59 -0.12684626959651268 1104 60-61 -0.1750066662009715 1104 62-63 -0.19042482364977076 1104 64-65 -0.04560286543563308 1104 66-67 -0.21023086253295986 1104 68-69 -0.3889224378866345 1104 70-71 -0.32357545734412696 1104 72-73 -0.21138351313642545 1104 74-75 -0.14917793437933113 1104 76-77 -0.2786089672312819 1104 78-79 -0.2561929528136808 1104 80-81 -0.12389004059793507 1104 82-83 -0.15708714742931917 1104 84-85 -0.2753519251080583 1104 86-87 -0.04675252306381594 1104 88-89 0.006425864141711202 1104 90-91 0.24412906587103578 1104 92-93 0.03757301335487995 1104 94-95 -0.05225915196301045 1104 96-97 0.20192383734697827 1104 98-99 0.33928747992843 1104 100-101 0.2825864278075514 1105 1 -0.0923126523996558 1105 2 0.021178870805925953 1105 3 -0.056641591735278496 1105 4 -0.033634490298425135 1105 5 -0.1707479829433396 1105 6 -0.08429747076367988 1105 7 0.005525889336425394 1105 8 0.018600098143274124 1105 9 -0.022202295618228618 1105 10-11 -0.01551525562580025 1105 12-13 -0.025208123387642445 1105 14-15 -0.053517132726234706 1105 16-17 -0.06826981402648613 1105 18-19 -0.06507837592749155 1105 20-21 -0.10834213552510619 1105 22-23 -0.07562821272245657 1105 24-25 -0.10703497372330872 1105 26-27 -0.06743210212739825 1105 28-29 -0.024504787925380356 1105 30-31 0.0031304107334406694 1105 32-33 -0.0403425694744044 1105 34-35 -0.05726548909377982 1105 36-37 0.05937298470754371 1105 38-39 0.00523227854135655 1105 40-41 -0.09891178383353605 1105 42-43 -0.10049955759738083 1105 44-45 -0.06986009152372219 1105 46-47 -0.19592831505138975 1105 48-49 -0.29072097236288386 1105 50-51 -0.16346663610333678 1105 52-53 -0.14557766050098309 1105 54-55 -0.15757038532156997 1105 56-57 -0.19553556027898367 1105 58-59 0.009929967359177283 1105 60-61 0.04731379635588695 1105 62-63 -0.12393082083440987 1105 64-65 0.10013824639935365 1105 66-67 -0.04938748057066533 1105 68-69 -0.14786331821051135 1105 70-71 -0.14555839648811286 1105 72-73 0.14580319552798215 1105 74-75 0.11993275953351201 1105 76-77 0.07107520140342416 1105 78-79 0.06965589699882102 1105 80-81 0.10501486728236387 1105 82-83 0.08625957438119869 1105 84-85 0.025070330593433 1105 86-87 -0.10870198132007403 1105 88-89 -0.027758500327347946 1105 90-91 -0.11256611796119387 1105 92-93 0.017964129455567246 1105 94-95 -0.047820643949314956 1105 96-97 0.0694126932090171 1105 98-99 0.020935474468334547 1105 100-101 0.0023309900111385673 1106 1 -0.203139661028505 1106 2 -0.049340755727001806 1106 3 -0.14288131997248144 1106 4 -0.08322128687636621 1106 5 -0.13070649956253533 1106 6 -0.09088078361777718 1106 7 -0.056500619413448305 1106 8 -0.0971718842973388 1106 9 -0.2334369537528076 1106 10-11 -0.1870974696262806 1106 12-13 -0.31666161377884094 1106 14-15 -0.15897844843973985 1106 16-17 -0.08608834173231372 1106 18-19 -0.10963960725857902 1106 20-21 -0.364955736443207 1106 22-23 -0.24261605216417337 1106 24-25 -0.17523070380880057 1106 26-27 -0.03450268034178805 1106 28-29 -0.16803601299737636 1106 30-31 -0.04829567326775219 1106 32-33 -0.13791414275441838 1106 34-35 -0.02689047116616905 1106 36-37 -0.014724344826326785 1106 38-39 0.1182856255646314 1106 40-41 -0.07063134790809755 1106 42-43 -0.25683965133264053 1106 44-45 -0.16897841061967256 1106 46-47 -0.03731230858498691 1106 48-49 -0.08351760915504514 1106 50-51 -0.011535211991706262 1106 52-53 -0.002034638327465643 1106 54-55 -0.1594092750897218 1106 56-57 -0.1424402526887789 1106 58-59 -0.1938669496472869 1106 60-61 -0.029704044838162247 1106 62-63 -0.11313806356547218 1106 64-65 -0.1427366356627715 1106 66-67 0.06577980100335168 1106 68-69 0.21169523690169711 1106 70-71 0.09673378829002388 1106 72-73 0.08148977225567222 1106 74-75 0.1291804941586463 1106 76-77 0.20374235639056337 1106 78-79 0.03244850156819368 1106 80-81 0.23790378126770761 1106 82-83 0.11173733710993972 1106 84-85 0.0812564787814658 1106 86-87 0.3608693173128188 1106 88-89 0.3298108513861351 1106 90-91 0.25124284004192177 1106 92-93 0.31336222596009833 1106 94-95 0.20281765877385638 1106 96-97 0.34490218676342366 1106 98-99 0.2530271429840987 1106 100-101 0.2686015126598491 1107 1 -0.137905875206485 1107 2 -0.03697884630626902 1107 3 -0.0788195206165625 1107 4 -0.03173541186107798 1107 5 -0.10267054505599305 1107 6 -0.07508444429202399 1107 7 -0.12943894235176856 1107 8 0.022258632191999084 1107 9 -0.17041465543638878 1107 10-11 -0.0803804584975083 1107 12-13 -0.1271463066164742 1107 14-15 -0.1367208967704201 1107 16-17 -0.11418544335882785 1107 18-19 -0.1501342874591387 1107 20-21 -0.13707570709259187 1107 22-23 -0.13534362136232403 1107 24-25 -0.20683357364696775 1107 26-27 -0.149214677406718 1107 28-29 -0.2511960961574573 1107 30-31 -0.2055915437989242 1107 32-33 -0.1641258748485086 1107 34-35 -0.13440749044789158 1107 36-37 -0.21498625832353468 1107 38-39 -0.22224268555191884 1107 40-41 -0.3284123189243573 1107 42-43 -0.31864410284297406 1107 44-45 -0.432461350573206 1107 46-47 -0.41201166857847227 1107 48-49 -0.43179631772786564 1107 50-51 -0.379508388788409 1107 52-53 -0.4485716730997993 1107 54-55 -0.47830169692332447 1107 56-57 -0.3404957742737196 1107 58-59 -0.3974308076229107 1107 60-61 -0.3690247003352809 1107 62-63 -0.27071419172983724 1107 64-65 -0.3255716199683505 1107 66-67 -0.26811404759637725 1107 68-69 -0.24171065768067024 1107 70-71 -0.45821199459693673 1107 72-73 -0.2405313438830916 1107 74-75 -0.3206890586520821 1107 76-77 -0.46319947231597425 1107 78-79 -0.39029211234888805 1107 80-81 -0.16655893937148392 1107 82-83 -0.19410527020325574 1107 84-85 -0.32727762092910595 1107 86-87 -0.1357472087154754 1107 88-89 -0.14731503015151048 1107 90-91 -0.11309717870986091 1107 92-93 -0.13692516550376155 1107 94-95 -0.2707769765854877 1107 96-97 -0.015468364783597366 1107 98-99 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0.20689480644759328 2316 52-53 0.16123505184888387 2316 54-55 0.2700570719401796 2316 56-57 0.44856093591864266 2316 58-59 0.04136405979971869 2316 60-61 0.06822567185199802 2316 62-63 0.44801879750788487 2316 64-65 0.19714574068277102 2316 66-67 0.11795565471937408 2316 68-69 0.4175412294892027 2316 70-71 0.4088130321283998 2316 72-73 0.5431229121686343 2316 74-75 0.5944342058402654 2316 76-77 0.6337102255400993 2316 78-79 0.47279871446433575 2316 80-81 0.3592015031870659 2316 82-83 0.20947772919992858 2316 84-85 0.27349659419842354 2316 86-87 0.443920544453853 2316 88-89 0.33866564234335783 2316 90-91 0.12072087253721264 2316 92-93 0.33826781442108 2316 94-95 0.262719878677931 2316 96-97 -0.04740596848776235 2316 98-99 -0.06053033730190194 2316 100-101 -0.4635865113262838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 15.0 5 20.0 6 47.0 7 121.0 8 321.0 9 598.0 10 1029.0 11 1753.0 12 2756.0 13 4112.0 14 5268.0 15 7187.0 16 10259.0 17 14484.0 18 12818.0 19 7740.0 20 6356.0 21 6519.0 22 6714.0 23 7221.0 24 8214.0 25 9706.0 26 12089.0 27 14802.0 28 18758.0 29 23860.0 30 30610.0 31 38426.0 32 49988.0 33 66242.0 34 93137.0 35 136842.0 36 214422.0 37 341818.0 38 294901.0 39 30538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93871529162942 19.906813273597905 15.215021846343998 21.939449588428673 2 15.765400460635156 21.17901244579968 39.28225797731426 23.773329116250906 3 19.444804878839978 24.960667596587406 30.18369989511359 25.410827629459025 4 13.044571317351672 17.453314734918525 37.25589580562494 32.246218142104865 5 13.66043137848623 37.63429886126461 34.07358793567713 14.631681824572029 6 31.50147057233378 37.080116456353196 17.02836258258453 14.390050388728495 7 28.44530227830581 33.24331484304884 20.37553659670635 17.935846281938993 8 26.676155102129083 34.835263459521414 19.392902325883156 19.095679112466343 9 26.27843827380759 17.080265135541353 20.423519425611747 36.21777716503931 10-11 24.95326742790411 27.229207891350654 26.95452309122955 20.863001589515683 12-13 26.127894312297645 24.352656402437272 27.914047301153786 21.605401984111296 14-15 22.662391464192645 25.735049631816263 26.626617899892953 24.97594100409814 16-17 21.803836734031854 29.356334003741306 27.03251208356311 21.807317178663727 18-19 21.627280198094745 27.38177301283507 30.012076804965343 20.978869984104843 20-21 22.375068933078147 26.331692455747667 30.31403071982353 20.979207891350658 22-23 22.036424042463178 26.226378370487712 30.25440601426523 21.48279157278388 24-25 22.262812569799273 26.470215541841718 30.24448710354436 21.02248478481465 26-27 21.9031460371934 26.778385195087285 30.208134105486963 21.110334662232347 28-29 21.41220184893702 26.97310397449145 30.41646025677503 21.198233919796493 30-31 21.665583666258808 26.470422262027977 30.341211020855447 21.522783050857765 32-33 21.540809733891287 26.758874795904024 30.229317373298297 21.470998096906392 34-35 22.22858614201836 26.5991122500838 29.90654837209805 21.265753235799785 36-37 22.018741712813593 26.43489126805948 30.02982373382605 21.51654328530088 38-39 21.78306581162444 26.8206310363028 30.21778841962488 21.17851473244788 40-41 21.925888831219385 26.70616126555725 30.081009883111125 21.286940020112237 42-43 21.837079954119975 26.81102066831541 29.62407849620584 21.727820881358774 44-45 22.027775309176786 26.94746993553741 29.355769480950645 21.66898527433516 46-47 22.186787015711335 27.306250743395942 28.86339491354981 21.643567327342915 48-49 22.406021236794587 27.42874212000303 28.6157764838183 21.54946015938409 50-51 22.09129442804468 27.48094878948108 28.606416453109283 21.82134032936495 52-53 22.492184194840284 27.704444297266868 28.179474945495485 21.62389656239736 54-55 22.08761123906532 27.48544295585039 28.377991154939934 22.048954650144353 56-57 22.283462278738337 27.9161395313632 28.190216098441844 21.610182091456622 58-59 22.538176760631913 27.450503346633365 28.19971129204918 21.81160860068555 60-61 22.324112520409596 27.41495550437387 28.807707799439886 21.453224175776647 62-63 22.15083368475687 27.470338501962566 28.793786020912403 21.585041792368163 64-65 22.097647084265954 27.46439133443626 28.556845460148573 21.88111612114921 66-67 22.00566873195575 27.845550707712935 28.167643894421555 21.981136665909755 68-69 21.998775424141176 27.565290438035923 28.490210151274315 21.945723986548586 70-71 22.069026340545626 27.67473859495464 28.324061158508236 21.9321739059915 72-73 22.313333279268175 27.40339907656708 28.438983412809115 21.84428423135563 74-75 22.098322898757583 27.95442442231377 27.97118462170608 21.976068057222562 76-77 22.170026816319027 27.634291097630864 28.157743212119247 22.037938873930862 78-79 22.164079648792725 27.660512699905926 28.12736535072069 22.048042300580658 80-81 22.025098398589982 28.1165185281301 27.971049458807755 21.887333614472162 82-83 22.204162896172242 27.784145473615773 27.99270253046075 22.01898909975123 84-85 21.978846323449655 27.782303859138587 27.850865262481367 22.38798455493039 86-87 21.998775424141176 27.803143348363445 28.02244515089586 22.175636076599517 88-89 21.989314021258423 27.667135681923856 27.868866307674008 22.474683989143713 90-91 22.016847445358334 27.66344850519221 27.99323993467027 22.326464114779178 92-93 22.117058925500615 27.47614057722181 28.055024933151714 22.351775564125866 94-95 21.922577340210424 27.40613612525816 27.96348033650155 22.707806198029864 96-97 21.92230701441377 27.414043154810173 27.991830754425234 22.67181907635082 98-99 21.887625247182825 27.622537126421093 27.710123986770057 22.779713639626024 100-101 21.499787091672243 27.757737358990475 27.941379240424197 22.801096308913085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 564.0 1 401.5 2 315.0 3 425.0 4 384.5 5 348.0 6 650.5 7 1442.0 8 2253.5 9 2701.5 10 2496.5 11 2098.0 12 2163.0 13 2333.5 14 2488.0 15 2626.5 16 2704.5 17 2812.5 18 2927.5 19 3043.0 20 3253.5 21 3597.0 22 4170.5 23 4979.0 24 6047.0 25 7338.5 26 9142.0 27 11341.5 28 13067.0 29 14791.5 30 17099.0 31 19615.0 32 22584.5 33 25650.5 34 29070.0 35 32245.0 36 35980.0 37 39527.0 38 41806.0 39 42695.5 40 43056.5 41 45944.0 42 49190.5 43 51823.5 44 53963.5 45 55393.5 46 54888.0 47 56645.0 48 64331.5 49 64553.0 50 59384.0 51 56229.0 52 51510.0 53 49245.0 54 47206.5 55 42674.5 56 37792.0 57 32903.5 58 28534.5 59 25004.0 60 21363.0 61 16875.5 62 13189.0 63 10302.5 64 7716.5 65 5896.5 66 4244.0 67 3222.5 68 2276.5 69 1340.5 70 970.5 71 768.0 72 632.5 73 510.0 74 407.5 75 334.5 76 207.0 77 113.5 78 64.5 79 41.0 80 27.0 81 13.5 82 7.5 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0493344578886474 2 0.0 3 0.0 4 0.0 5 0.01081303186600491 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006183702598371557 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.461402882754296E-4 24-25 4.392794195564494E-4 26-27 0.0016895362290632672 28-29 0.002365350720688574 30-31 7.096052162065722E-4 32-33 0.0 34-35 0.0 36-37 1.3516289832506137E-4 38-39 2.0274434748759204E-4 40-41 0.0 42-43 0.012502568095068176 44-45 3.379072458126534E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.3516289832506137E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.379072458126534E-4 84-85 3.379072458126534E-5 86-87 0.0 88-89 0.0 90-91 0.00841389042073507 92-93 0.002669467241919962 94-95 0.0 96-97 0.0 98-99 0.001486791881575675 100-101 0.012570149544230707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1479696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.34421561087207 #Duplication Level Percentage of deduplicated Percentage of total 1 78.95836803821179 43.698889449893834 2 12.12187152759773 13.4175094286132 3 3.760916902423123 6.244349878268353 4 1.612375569499456 3.569426446563221 5 0.8595874003337776 2.3786595210230796 6 0.5261639530158974 1.747207875742835 7 0.3646991913305009 1.412879347467415 8 0.2538114828209837 1.1237597943807742 9 0.1925314332690535 0.9589951039241453 >10 1.176877625519953 12.279880022971733 >50 0.10880579512231839 4.184309430667368 >100 0.05935058014423611 5.771389723911918 >500 0.0034193163135690487 1.1749062034821844 >1k 9.769475181625852E-4 1.0169589072593437 >5k 2.442368795406463E-4 1.0208788658305565 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8369 0.5655891480412192 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6736 0.45522864155880666 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4727 0.31945751019128255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2919 0.1972702501054271 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1709 0.11549669661876494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.758144916253068E-5 0.0 24-25 0.0 0.0 0.0 1.0137217374379603E-4 0.0 26-27 0.0 0.0 0.0 2.7032579665012274E-4 0.0 28-29 0.0 0.0 0.0 4.730701441377148E-4 0.0 30-31 0.0 0.0 0.0 0.0015881640553194712 0.0 32-33 0.0 0.0 0.0 0.0027370486910824926 0.0 34-35 0.0 0.0 0.0 0.003649398254776657 0.0 36-37 0.0 0.0 0.0 0.005913376801721435 0.0 38-39 0.0 0.0 0.0 0.008616634768222662 0.0 40-41 0.0 0.0 0.0 0.012570149544230707 0.0 42-43 0.0 0.0 0.0 0.016084384900682303 0.0 44-45 0.0 0.0 0.0 0.01821320054930202 0.0 46-47 0.0 0.0 0.0 0.021795017354916145 0.0 48-49 0.0 0.0 0.0 0.027032579665012272 0.0 50-51 0.0 0.0 0.0 0.03331765443712763 0.0 52-53 0.0 0.0 0.0 0.05068608687189801 0.0 54-55 0.0 0.0 0.0 0.06176944453455305 0.0 56-57 0.0 0.0 0.0 0.09099842129734756 0.0 58-59 0.0 0.0 0.0 0.13783236556698134 0.0 60-61 0.0 0.0 0.0 0.18409186751873358 0.0 62-63 0.0 0.0 0.0 0.2139966587731534 0.0 64-65 0.0 0.0 0.0 0.26356765173386965 0.0 66-67 0.0 0.0 0.0 0.3501056973864902 0.0 68-69 0.0 0.0 0.0 0.4715157708069766 0.0 70-71 0.0 0.0 0.0 0.6025899914577049 0.0 72-73 0.0 0.0 0.0 0.7390707280414355 0.0 74-75 0.0 0.0 0.0 0.9028881608114099 0.0 76-77 0.0 0.0 0.0 1.0388282458018403 0.0 78-79 0.0 0.0 0.0 1.2129856402936818 0.0 80-81 0.0 0.0 0.0 1.4412757755647105 0.0 82-83 0.0 0.0 0.0 1.6534477352104757 0.0 84-85 0.0 0.0 0.0 1.8997145359587373 0.0 86-87 0.0 0.0 0.0 2.214508926157805 0.0 88-89 0.0 0.0 0.0 2.6170578280944197 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6395 0.0 32.030632 1 GTATCAA 9590 0.0 31.221283 1 ATCAACG 11665 0.0 25.531523 3 TCAACGC 11715 0.0 25.422552 4 TATCAAC 11765 0.0 25.395258 2 CAACGCA 11890 0.0 25.048376 5 AACGCAG 12160 0.0 24.607729 6 GTACATG 6060 0.0 23.449165 1 GAGTACT 7435 0.0 23.002455 12-13 TACATGG 6240 0.0 22.455946 2 GTACTTT 7545 0.0 22.37922 14-15 ACGCAGA 13440 0.0 22.162611 7 GTATAGG 365 0.0 22.135273 1 CGCAGAG 13525 0.0 21.988201 8 GTACGGG 130 2.9433917E-4 21.921612 6 AGTACTT 7550 0.0 21.802633 12-13 AGAGTAC 10995 0.0 21.51725 10-11 ACATGGG 6315 0.0 21.361853 3 GCAGAGT 14175 0.0 20.577753 9 CAATCGG 60 0.0047670836 19.790342 74-75 >>END_MODULE