FastQCFastQC Report
Thu 26 May 2016
SRR937153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592546
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75110.4716347282904231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56560.35515457638272296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41440.26021226388437135No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24310.15264865190707208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23610.1482531744765928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90700.030.7709671
GGTATCA62000.026.4568251
TCAACGC108400.025.4737574
ATCAACG108400.025.471353
CAACGCA110200.025.1007985
AACGCAG110750.024.9643466
GTACATG66800.024.9116171
TATCAAC110950.024.8867152
TACATGG67400.023.973852
GAGTACT59350.022.81137312-13
ACGCAGA122400.022.6277717
ACATGGG67300.022.596443
CGCAGAG122800.022.4759868
AGTACTT62000.021.68313212-13
GTACTTT62750.021.6122114-15
GCAGAGT128550.020.9519589
AGAGTAC96950.020.9218710-11
GCGTCGA704.854882E-420.356844-45
GTATAGA4700.019.2205621
GTCTAGC2854.2284228E-818.3509311
Produced by FastQC (version 0.11.5)