##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937153_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1592546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.572755826205334 33.0 31.0 34.0 30.0 34.0 2 31.67211559352132 33.0 31.0 34.0 30.0 34.0 3 31.718669978763565 33.0 31.0 34.0 30.0 34.0 4 35.34571874219018 37.0 35.0 37.0 33.0 37.0 5 35.339259274143416 37.0 35.0 37.0 33.0 37.0 6 35.38541053131275 37.0 35.0 37.0 33.0 37.0 7 35.32424683494229 37.0 35.0 37.0 33.0 37.0 8 35.30544235456935 37.0 35.0 37.0 33.0 37.0 9 36.91027825883836 39.0 37.0 39.0 33.0 39.0 10-11 36.806557549985996 39.0 37.0 39.0 33.0 39.0 12-13 36.68889250294811 39.0 37.0 39.0 32.0 39.0 14-15 37.82653939038495 40.0 38.0 41.0 33.0 41.0 16-17 37.77075701424009 40.0 38.0 41.0 33.0 41.0 18-19 37.727091713520366 40.0 38.0 41.0 32.0 41.0 20-21 37.6567128987169 40.0 37.5 41.0 32.0 41.0 22-23 37.51668115081134 40.0 37.0 41.0 32.0 41.0 24-25 37.35587072524122 40.0 37.0 41.0 32.0 41.0 26-27 37.192152377388155 39.5 37.0 41.0 31.5 41.0 28-29 37.00183322805118 39.0 36.0 41.0 31.0 41.0 30-31 36.995870448954065 39.0 36.5 41.0 31.0 41.0 32-33 36.858957920210784 39.0 36.0 41.0 31.0 41.0 34-35 36.645076814107725 39.0 36.0 41.0 30.0 41.0 36-37 36.650045587380205 39.0 36.0 41.0 30.0 41.0 38-39 36.63110202154286 39.0 36.0 41.0 30.0 41.0 40-41 36.471408361202755 39.0 35.5 41.0 30.0 41.0 42-43 36.24341274914508 39.0 35.0 41.0 30.0 41.0 44-45 36.02181224278608 39.0 35.0 41.0 29.0 41.0 46-47 35.69437240745322 39.0 35.0 41.0 27.5 41.0 48-49 35.392220695665934 39.0 35.0 40.5 26.5 41.0 50-51 34.478146314141 38.0 34.0 39.5 25.0 40.5 52-53 34.620469047675854 38.0 34.5 39.5 25.5 40.5 54-55 34.96595891107698 39.0 35.0 40.0 26.0 41.0 56-57 34.74048316343766 38.0 34.0 40.0 24.5 41.0 58-59 34.501825692947016 38.0 34.0 40.0 24.0 41.0 60-61 34.21954310895886 38.0 34.0 40.0 23.0 41.0 62-63 33.901632668695285 37.5 34.0 40.0 22.5 41.0 64-65 33.54139566455223 37.0 33.0 40.0 22.0 41.0 66-67 33.14989300152084 36.5 33.0 39.0 20.5 41.0 68-69 32.64959348113022 36.0 32.0 39.0 18.5 40.5 70-71 32.215830814306145 35.0 32.0 39.0 18.0 40.0 72-73 31.803221382616265 35.0 31.0 38.0 14.5 40.0 74-75 31.344891136582554 35.0 31.0 37.0 11.0 39.0 76-77 30.907319474602303 35.0 31.0 37.0 7.5 39.0 78-79 30.434486036824055 34.5 30.0 36.0 7.0 39.0 80-81 29.97527952096831 34.0 30.0 36.0 2.0 37.5 82-83 29.531281357022024 34.0 29.0 35.0 2.0 37.0 84-85 29.072022723362466 34.0 29.0 35.0 2.0 36.5 86-87 28.647203911221403 34.0 29.0 35.0 2.0 36.0 88-89 28.253623443216085 34.0 28.5 35.0 2.0 36.0 90-91 27.81981493784167 34.0 27.0 35.0 2.0 35.0 92-93 27.3691315666863 33.0 26.5 35.0 2.0 35.0 94-95 26.82046703831475 33.0 25.5 35.0 2.0 35.0 96-97 26.186253960639128 33.0 24.5 35.0 2.0 35.0 98-99 25.470771330938007 32.0 21.5 34.5 2.0 35.0 100-101 23.643200259207582 30.5 9.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09356026490341307 1101 2 -0.10737948293554922 1101 3 -0.07533481206735715 1101 4 -0.11582868112034106 1101 5 -0.0231314670628322 1101 6 -0.14154709521995557 1101 7 -0.015635218296793596 1101 8 -0.1863685925446248 1101 9 -0.1517910013552779 1101 10-11 -0.09021590253940559 1101 12-13 0.008834908700855237 1101 14-15 -0.09437296462484568 1101 16-17 -0.16247365340232278 1101 18-19 -0.03427880219854984 1101 20-21 -0.030877186025570325 1101 22-23 -0.14549308819702844 1101 24-25 -0.006052350369863291 1101 26-27 0.04666901870488971 1101 28-29 -0.03568787567882481 1101 30-31 -0.08200744922167047 1101 32-33 -0.092864708576613 1101 34-35 -0.2328863860667738 1101 36-37 -0.2554404108606292 1101 38-39 -0.1463184301916698 1101 40-41 -0.10257203259369163 1101 42-43 -0.07880981037931889 1101 44-45 -0.24968222383865424 1101 46-47 -0.08468209230849766 1101 48-49 -0.12949114674324136 1101 50-51 -0.1211136567419544 1101 52-53 -0.1806850111143561 1101 54-55 -0.018128797560258647 1101 56-57 0.11676069809286815 1101 58-59 0.1557391738064382 1101 60-61 0.039694275050514705 1101 62-63 0.05668062719902878 1101 64-65 -0.04757186821546355 1101 66-67 0.021979119610854525 1101 68-69 -0.29969002375874965 1101 70-71 -0.1572142035498061 1101 72-73 -0.23683508051381352 1101 74-75 -0.13449659256511381 1101 76-77 -0.16858940807688683 1101 78-79 -0.06786648509532256 1101 80-81 -0.05918878112982284 1101 82-83 -0.050639709131854005 1101 84-85 0.059157870290967196 1101 86-87 0.19795420804317487 1101 88-89 0.02781966507538769 1101 90-91 0.014691279135618629 1101 92-93 -0.05628327630194363 1101 94-95 -0.12352463354069698 1101 96-97 -0.14347725023180757 1101 98-99 -0.0835654954424534 1101 100-101 0.32447932270387625 1102 1 0.010172350088478055 1102 2 -0.13383085951649676 1102 3 -0.07063707743658298 1102 4 -0.1107639644109355 1102 5 -0.061386789213031534 1102 6 0.060699390238411866 1102 7 0.09553600739604207 1102 8 0.11690057390779884 1102 9 0.16717353129429569 1102 10-11 -0.02262647198728729 1102 12-13 0.10765956498221385 1102 14-15 0.1276225260231385 1102 16-17 0.20898433249627857 1102 18-19 0.13540604369399745 1102 20-21 0.17571211029255096 1102 22-23 0.20490119571013565 1102 24-25 0.06290239912448925 1102 26-27 0.20281898309875857 1102 28-29 0.17329943124605052 1102 30-31 0.08132448868487785 1102 32-33 -0.09753059107409001 1102 34-35 -0.04595065229600692 1102 36-37 -0.0069813715664750475 1102 38-39 0.17556114144263546 1102 40-41 0.12534624848318288 1102 42-43 0.06479792149851704 1102 44-45 0.022010216512533987 1102 46-47 0.008967502373110392 1102 48-49 0.11804270183626642 1102 50-51 0.1103487429522545 1102 52-53 0.09672977874119226 1102 54-55 0.21515372403662525 1102 56-57 0.21556476424519389 1102 58-59 -0.0030206178806153616 1102 60-61 0.12497039891891148 1102 62-63 0.20860620241442973 1102 64-65 0.1747908320263818 1102 66-67 0.2655953833101208 1102 68-69 0.19478055965857521 1102 70-71 0.11462896564644609 1102 72-73 0.029054128665968904 1102 74-75 -0.03045068909073123 1102 76-77 -0.0516481684373602 1102 78-79 0.07053183038185651 1102 80-81 0.0668724068182378 1102 82-83 0.3497294343415298 1102 84-85 0.2224920833498878 1102 86-87 0.16776122961844564 1102 88-89 0.1786558973320389 1102 90-91 0.16370420427628218 1102 92-93 -0.006591559449208972 1102 94-95 -0.22389172937694468 1102 96-97 -0.09156976247781756 1102 98-99 0.06976043606180937 1102 100-101 0.07475074838260909 1103 1 -0.05813132036072588 1103 2 -0.12388440353615593 1103 3 0.002661668914708315 1103 4 -0.11257146323536205 1103 5 -0.2333507057784061 1103 6 -0.1600020053662803 1103 7 -0.08081501424337034 1103 8 0.036084815020025474 1103 9 0.04051730430668954 1103 10-11 -0.059452624714175784 1103 12-13 -0.031907558013770654 1103 14-15 -0.05554859303356352 1103 16-17 0.10579742083725563 1103 18-19 0.012507657556007246 1103 20-21 -0.052476797622382776 1103 22-23 0.00721674166395303 1103 24-25 -0.0057194523363293115 1103 26-27 -0.013235794163449555 1103 28-29 -0.306092775370864 1103 30-31 -0.24662054329957783 1103 32-33 -0.14748811518443006 1103 34-35 0.01207340924190703 1103 36-37 0.048746219351897935 1103 38-39 -0.063641221237134 1103 40-41 0.08525399927947319 1103 42-43 0.03575916308097504 1103 44-45 -0.04546378779937754 1103 46-47 -0.1806515232765662 1103 48-49 -0.05142489671222705 1103 50-51 -0.14571732424614936 1103 52-53 -0.08185216978201737 1103 54-55 -0.16733134840528407 1103 56-57 -0.1701358328571274 1103 58-59 -0.06947841703065194 1103 60-61 -0.19439616689986394 1103 62-63 -0.051875804665627356 1103 64-65 -0.04654836734191292 1103 66-67 0.06530193369118109 1103 68-69 0.09994487370068583 1103 70-71 -0.025339626593883224 1103 72-73 0.07395391661923156 1103 74-75 0.015098658355391592 1103 76-77 -0.016337617559454287 1103 78-79 0.04017232687288441 1103 80-81 -0.0923056015394188 1103 82-83 0.1392206996128884 1103 84-85 -0.05995605835766682 1103 86-87 0.13316666919400078 1103 88-89 0.019207063589103512 1103 90-91 0.06498058812524121 1103 92-93 0.1356894734579619 1103 94-95 0.0360214556715448 1103 96-97 0.11375724642104856 1103 98-99 0.33770733121845 1103 100-101 0.6238587546996506 1104 1 -0.13245394978530456 1104 2 -0.24828166604428858 1104 3 -0.10126999660853286 1104 4 -0.24877883506943732 1104 5 -0.16605705916339986 1104 6 -0.13857357955706107 1104 7 -0.18525789866136222 1104 8 -0.06089586901612165 1104 9 -0.008851771359907445 1104 10-11 -0.0719994255076628 1104 12-13 -0.06356412882745133 1104 14-15 0.017545737156673624 1104 16-17 -0.10116313353940853 1104 18-19 -0.23544081902498704 1104 20-21 -0.09544488976321475 1104 22-23 -0.13363868908949428 1104 24-25 -0.12130267620189983 1104 26-27 -0.036205854064917276 1104 28-29 -0.03285392113938457 1104 30-31 -0.08110450693678217 1104 32-33 -0.06890588229020977 1104 34-35 0.08894326935362784 1104 36-37 0.0034249026541175454 1104 38-39 -0.02354976025463884 1104 40-41 -0.10575295790667383 1104 42-43 -0.07613007770024893 1104 44-45 0.16530675446936272 1104 46-47 0.12135763124229015 1104 48-49 0.19058251607254562 1104 50-51 0.08718609327543447 1104 52-53 0.1103949419531034 1104 54-55 0.12512774078745537 1104 56-57 0.03966556513930186 1104 58-59 -0.10409261185313312 1104 60-61 0.003555899514715577 1104 62-63 0.1524975211866746 1104 64-65 0.13385395550201906 1104 66-67 0.0411137679100122 1104 68-69 0.09768970478349814 1104 70-71 0.06620201636215484 1104 72-73 0.18114900679053392 1104 74-75 0.03153045683542999 1104 76-77 0.22347733759864852 1104 78-79 0.21125360247814484 1104 80-81 0.1958737190690627 1104 82-83 0.22900952875985325 1104 84-85 0.30882965162278 1104 86-87 0.26173511889998835 1104 88-89 0.17048409149053256 1104 90-91 0.30378396363246907 1104 92-93 0.2401885321238204 1104 94-95 0.11947821723391172 1104 96-97 0.12901459317060926 1104 98-99 0.22436930451008763 1104 100-101 0.5556538000201918 1105 1 0.1294598953903403 1105 2 0.22269955429315402 1105 3 0.141964101990375 1105 4 0.17535314485652975 1105 5 0.15956003555240983 1105 6 0.09124452794345217 1105 7 0.20640592948315373 1105 8 0.16558413643384284 1105 9 0.12210401581905472 1105 10-11 0.27808573015689575 1105 12-13 0.31771184365024396 1105 14-15 0.3203104577325533 1105 16-17 0.2632815379868063 1105 18-19 0.33606836643269844 1105 20-21 0.3133647475871939 1105 22-23 0.20562034308800037 1105 24-25 0.305097850627817 1105 26-27 0.22734070905246284 1105 28-29 0.2905570741600201 1105 30-31 0.18986649472090988 1105 32-33 0.19933900269287363 1105 34-35 0.29085529787509756 1105 36-37 0.30399714871992956 1105 38-39 0.32524102930868537 1105 40-41 0.33475956565306575 1105 42-43 0.32692303672626366 1105 44-45 0.3483244441394149 1105 46-47 0.09261021429744432 1105 48-49 0.18291623544488544 1105 50-51 0.13171796420215287 1105 52-53 0.14714017891293452 1105 54-55 0.242751557123718 1105 56-57 0.1458365122973717 1105 58-59 0.12246460139935778 1105 60-61 0.4192222709750766 1105 62-63 0.38659044304783663 1105 64-65 0.31014775389881777 1105 66-67 0.3847168743886513 1105 68-69 0.3556012337250678 1105 70-71 0.22389327598474296 1105 72-73 0.31481955655550564 1105 74-75 0.3525480102564167 1105 76-77 0.28173062460730236 1105 78-79 0.4109606669920751 1105 80-81 0.3731610002190031 1105 82-83 0.396337003383465 1105 84-85 0.36780278784913634 1105 86-87 0.2909185328473498 1105 88-89 0.503374615368184 1105 90-91 0.49262289453148256 1105 92-93 0.3634984760346214 1105 94-95 0.5238359164413957 1105 96-97 0.6715504153201657 1105 98-99 0.5456775225994228 1105 100-101 0.8735580623450545 1106 1 -0.09157978286165402 1106 2 0.05601659441732565 1106 3 -0.10578443376464364 1106 4 0.051794879483033185 1106 5 0.025708749675189324 1106 6 -0.015583184232717429 1106 7 0.02618331279376207 1106 8 -0.10331813003730872 1106 9 -0.06391383810803575 1106 10-11 -0.1861703990911323 1106 12-13 -0.3943164036292188 1106 14-15 -0.3929689365030882 1106 16-17 -0.40216270922405783 1106 18-19 -0.18618429836454453 1106 20-21 -0.10998213483586028 1106 22-23 -0.13301728736486496 1106 24-25 -0.23697807572688134 1106 26-27 0.023553824228301323 1106 28-29 0.15711183220586378 1106 30-31 0.10988184463901973 1106 32-33 0.2313868875770666 1106 34-35 0.10750610010126138 1106 36-37 -0.1432745962827795 1106 38-39 -0.346946462651907 1106 40-41 -0.12936235900370718 1106 42-43 -0.19118600771989946 1106 44-45 -0.04856055153002359 1106 46-47 -0.19752424755500897 1106 48-49 -0.017371039558760515 1106 50-51 -0.17555438107667953 1106 52-53 -0.18852865732316104 1106 54-55 -0.007316617093565014 1106 56-57 -0.3349507927200719 1106 58-59 -0.36112299175063356 1106 60-61 -0.05846718045829391 1106 62-63 0.16836220091359877 1106 64-65 0.1618491079502533 1106 66-67 0.06085718464565559 1106 68-69 0.03521371260821127 1106 70-71 -0.03595982787160068 1106 72-73 0.10709786171873503 1106 74-75 0.06928756759565147 1106 76-77 0.08100514803093617 1106 78-79 0.03465185065084597 1106 80-81 0.08204165674957764 1106 82-83 -0.08395164367989949 1106 84-85 0.030927825366394046 1106 86-87 0.20357106729891328 1106 88-89 0.2584105033052033 1106 90-91 0.3567538233301626 1106 92-93 0.4597844818830268 1106 94-95 0.41970258653821446 1106 96-97 0.47462254278451255 1106 98-99 0.4902330181273662 1106 100-101 0.7714815357142335 1107 1 -0.1314570317669954 1107 2 -0.18383909981583102 1107 3 -0.17620935096568857 1107 4 -0.139304698957325 1107 5 -0.013535676162227617 1107 6 0.019921505981045584 1107 7 -0.1557491741041801 1107 8 -0.07555985739557514 1107 9 -0.022210521590110943 1107 10-11 -0.09553908208684447 1107 12-13 -0.16705858626085046 1107 14-15 0.06274878572074982 1107 16-17 -0.07411206679810078 1107 18-19 0.10758557234888144 1107 20-21 0.01286609401948624 1107 22-23 0.02217469429707819 1107 24-25 0.08436685291471946 1107 26-27 0.06750228092758448 1107 28-29 -0.0613549950154777 1107 30-31 -0.015375118832722023 1107 32-33 -0.0874646076401433 1107 34-35 0.14096558353526234 1107 36-37 -0.01646320933634371 1107 38-39 -0.021375298381812513 1107 40-41 0.12435008785227808 1107 42-43 0.0076731145351587315 1107 44-45 0.05653768112502888 1107 46-47 0.015235013009196052 1107 48-49 0.19777705900136056 1107 50-51 0.1336455951579154 1107 52-53 0.018771053484982758 1107 54-55 0.003089315415444105 1107 56-57 -0.08149741644320585 1107 58-59 -0.03173775587205796 1107 60-61 -0.1297946304714941 1107 62-63 0.07289945940800635 1107 64-65 0.22268039980742316 1107 66-67 0.03728659793024036 1107 68-69 -0.10370200447842848 1107 70-71 0.1568754543633446 1107 72-73 0.13570008267899425 1107 74-75 -0.002426346636099197 1107 76-77 -0.037414424716775585 1107 78-79 0.03163212398067117 1107 80-81 0.04814983633315961 1107 82-83 0.2558547235149966 1107 84-85 0.2938518548189215 1107 86-87 0.19823324617228977 1107 88-89 0.06921312468494989 1107 90-91 0.13285618591567427 1107 92-93 0.16970018377085694 1107 94-95 0.041696780593817095 1107 96-97 0.21759772313217596 1107 98-99 0.5106628403980373 1107 100-101 0.6510888814359745 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44-45 -0.77510207406322 2316 46-47 -0.47460924396679616 2316 48-49 -0.6485889371050604 2316 50-51 -0.5867590949465509 2316 52-53 -0.5890281856136426 2316 54-55 -0.923980259981235 2316 56-57 -0.7383773390806567 2316 58-59 -1.006857044340201 2316 60-61 -0.915072524886881 2316 62-63 -0.8483361146399133 2316 64-65 -1.091429676409959 2316 66-67 -1.0971594582339534 2316 68-69 -1.0291101300907712 2316 70-71 -1.0546840753537978 2316 72-73 -1.500088048277476 2316 74-75 -1.4356321208341605 2316 76-77 -1.2473560847032878 2316 78-79 -1.0269687336238889 2316 80-81 -1.0365701061538921 2316 82-83 -0.9937482844630132 2316 84-85 -0.9534382292874035 2316 86-87 -1.3677800377915226 2316 88-89 -1.4261740697775203 2316 90-91 -1.5242352749386257 2316 92-93 -1.4995809680149712 2316 94-95 -1.9990101705837944 2316 96-97 -2.40215644719267 2316 98-99 -2.541124578008649 2316 100-101 -3.0678025946872474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7481.0 3 1605.0 4 1571.0 5 1590.0 6 1816.0 7 2137.0 8 2365.0 9 3092.0 10 3708.0 11 4710.0 12 6033.0 13 7480.0 14 8565.0 15 10049.0 16 12615.0 17 16031.0 18 13674.0 19 10059.0 20 10173.0 21 10564.0 22 11293.0 23 12574.0 24 14178.0 25 15991.0 26 18876.0 27 21996.0 28 26024.0 29 31217.0 30 37943.0 31 47643.0 32 60473.0 33 78905.0 34 107143.0 35 151902.0 36 221921.0 37 310772.0 38 253493.0 39 34869.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.598974086938 19.03621881357224 14.73297846804795 22.631828631441802 2 15.066240597336773 20.29910923463707 40.25511258004544 24.379537587980714 3 19.12207324684518 24.583876272272043 29.95272101367356 26.341329467209217 4 12.438381839560702 16.593179380565473 37.76331194738894 33.205126832484886 5 13.62136517799907 37.258271506673 34.315717090605915 14.804646224722015 6 31.474379628557475 36.39757892776052 17.607334706063003 14.520706737619001 7 28.529908957142013 32.716898626867774 20.661744251763146 18.091448164227067 8 26.37043889790935 34.40357965487294 19.9181235761306 19.307857871087112 9 26.23332326436087 16.913545285203647 20.57990988269647 36.273221567739014 10-11 24.74987113572382 26.78193017601696 27.441262120951787 21.026936567307434 12-13 25.872253384836874 24.05934970222876 28.032979049806862 22.035417863127503 14-15 22.596585705192926 25.343614581560264 26.707627213299507 25.3521724999473 16-17 21.85644212397133 29.066527035497703 26.97882498357404 22.09820585695693 18-19 21.696857955948772 27.27513847615662 29.732203395163204 21.295800172731404 20-21 22.495460507913396 26.206375331592945 29.784700173273144 21.513463987220515 22-23 22.12589357631897 26.233166784132102 29.813827778896496 21.827111860652437 24-25 22.173632218486386 26.417364291527857 29.80462938289262 21.60437410709314 26-27 21.837200126113643 26.67448269178367 29.794064729525882 21.694252452576805 28-29 21.568083904008297 26.80358880189094 29.981391444217753 21.64693584988301 30-31 21.64695153668506 26.43443815891703 29.955375447590775 21.96323485680714 32-33 21.545224312135048 26.702244694764055 29.784467512785557 21.968063480315337 34-35 22.218568863778955 26.604791010766466 29.431892623565147 21.74474750188943 36-37 22.013370154168634 26.386342519978616 29.6523615183466 21.947925807506152 38-39 21.757857798589423 26.765247932870274 29.722088164396354 21.75480610414395 40-41 21.947425795683888 26.651859099496466 29.692360207704443 21.708354897115196 42-43 21.764656100236678 26.679622231094175 29.28819587001271 22.267525798656436 44-45 21.90976287653211 26.881177728851885 29.09646767560853 22.11259171900748 46-47 21.989150420403906 27.12555358529461 28.717280419032132 22.16801557526935 48-49 22.218282038752317 27.198771837470474 28.451980928113535 22.130965195663673 50-51 21.864751428781812 27.27646904447679 28.602540937643372 22.25623858909802 52-53 22.266133686806985 27.464229360090584 28.179988330837745 22.089648622264686 54-55 21.904323433319508 27.18067687822599 28.392368582870787 22.522631105583713 56-57 22.08698716002699 27.620932160333716 28.240736517084574 22.05134416255472 58-59 22.371530511493688 27.139616863347428 28.235623096403188 22.253229528755693 60-61 22.19445574292686 27.13111136486821 28.788260567436524 21.886172324768403 62-63 22.03232060510142 27.25495062820105 28.75122017818455 21.961508588512977 64-65 21.883183698301046 27.246452216874907 28.523726492682975 22.346637592141068 66-67 21.80829342540001 27.592977462163752 28.153859318950747 22.444869793485488 68-69 21.80692237711818 27.35920636897954 28.550495956473103 22.28337529742918 70-71 21.90848214987923 27.373184268261763 28.365588191066536 22.35274539079247 72-73 22.033903637854426 27.18454696879025 28.517019465110614 22.264529928244702 74-75 21.74245696537001 27.531875843318932 28.282128858660926 22.44353833265013 76-77 21.93748615613862 27.34011598638771 28.26875591510441 22.453641942369263 78-79 21.89943065406823 27.47100413291786 28.23576383470342 22.39380137831049 80-81 21.815365420835054 27.758733126818502 28.100561629325902 22.325339823020542 82-83 21.945754958146587 27.5826331545721 28.105230535921883 22.36638135135943 84-85 21.829334658710213 27.548451227913446 27.907781451224768 22.714432662151577 86-87 21.928861767517713 27.430314046477335 28.23398285577846 22.4068413302265 88-89 21.883160275198303 27.29362329747164 28.01883776916717 22.804378658162886 90-91 21.819809746848225 27.339554716893282 28.239508690442072 22.60112684581642 92-93 22.017137203020955 27.126962228622936 28.302624065390052 22.553276502966053 94-95 21.791037544706565 27.030923981848066 28.229223559259324 22.948814914186045 96-97 21.746820175335113 27.05629605440944 28.251339457195414 22.945544313060033 98-99 21.71353459770093 27.20712183648544 27.97676015083816 23.102583414975463 100-101 21.666037017103086 26.898031687153605 28.47405345740523 22.961877838338076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2363.0 1 1366.0 2 450.5 3 522.0 4 422.5 5 392.5 6 696.5 7 1500.0 8 2409.0 9 2792.0 10 2461.5 11 2042.5 12 2076.0 13 2209.0 14 2314.0 15 2446.0 16 2527.0 17 2599.0 18 2703.5 19 2858.0 20 3119.0 21 3602.5 22 4320.0 23 5147.5 24 6198.5 25 7633.5 26 9635.5 27 11904.0 28 13586.5 29 15437.5 30 18073.0 31 20803.5 32 23873.5 33 27241.0 34 31367.0 35 34578.0 36 37672.0 37 41661.0 38 44299.0 39 45114.5 40 46111.5 41 49449.0 42 52781.5 43 55489.5 44 57454.5 45 59097.0 46 59087.0 47 60907.5 48 68583.5 49 69090.5 50 64307.0 51 61045.5 52 56211.0 53 53805.5 54 51632.0 55 47167.5 56 41871.5 57 36299.0 58 31568.5 59 27532.0 60 23301.0 61 18743.0 62 14731.5 63 11419.5 64 8644.0 65 6563.5 66 4866.0 67 3833.0 68 2732.0 69 1715.0 70 1290.5 71 970.5 72 802.0 73 642.5 74 472.5 75 380.0 76 258.0 77 150.0 78 97.0 79 66.0 80 38.5 81 19.5 82 15.0 83 13.0 84 12.0 85 8.0 86 6.0 87 7.0 88 6.5 89 3.5 90 2.0 91 1.5 92 0.5 93 1.5 94 1.5 95 1.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33135620572341395 2 0.2949993281198785 3 0.29744823697400263 4 0.29782499218232944 5 0.28281757638397886 6 0.21964828645451998 7 0.22756014582938264 8 0.22391817881555698 9 0.22015062673228905 10-11 0.23013463975294904 12-13 0.2297264882772617 14-15 0.21189340841646018 16-17 0.2259275399266332 18-19 0.21183061588173907 20-21 0.21578654556917037 22-23 0.21512722395459846 24-25 0.2062420802915583 26-27 0.21996224912812565 28-29 0.21927153124619322 30-31 0.22746595702730094 32-33 0.22881599652380527 34-35 0.2163202821143 36-37 0.2140911471316998 38-39 0.2050176258644962 40-41 0.2052373997360202 42-43 0.20517460720129904 44-45 0.2211239110204666 46-47 0.21098291666300376 48-49 0.22011923046492848 50-51 0.2263670876696811 52-53 0.23503245746119733 54-55 0.23189283072514075 56-57 0.19927210893751265 58-59 0.18564612890302698 60-61 0.19462546136814884 62-63 0.19632085980561942 64-65 0.19261610025707265 66-67 0.20489204079505396 68-69 0.1941545173577404 70-71 0.19924071267015206 72-73 0.2131806553782434 74-75 0.2019093953958002 76-77 0.21311786284352227 78-79 0.21060616145467695 80-81 0.19795346570836886 82-83 0.21151665320813337 84-85 0.19220794878138528 86-87 0.19763950303476321 88-89 0.20671302430196678 90-91 0.20360479383327076 92-93 0.20806306379847114 94-95 0.20683860937140905 96-97 0.20027678949305078 98-99 0.20677581683668794 100-101 0.19073232421543868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1592546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.89762736571241 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09324437238958 45.57115572811165 2 11.595305358893942 13.194907270039913 3 3.463491689076073 5.911933785278769 4 1.4888838549288734 3.388558350742739 5 0.8084726589724266 2.300008804278991 6 0.49804336380517167 1.7002491435451559 7 0.3584594545912338 1.427684473113397 8 0.24210876391595823 1.1020331385010744 9 0.1869846701671478 0.9575085677643914 >10 1.101448048904118 11.878625417688777 >50 0.10362584479787369 4.125902280646958 >100 0.055737961139471964 5.567744625944241 >500 0.003311019803747234 1.235393386989005 >1k 6.622039607494468E-4 0.8114549558619389 >5k 2.2073465358314894E-4 0.8268400714931241 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7511 0.4716347282904231 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5656 0.35515457638272296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4144 0.26021226388437135 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2431 0.15264865190707208 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2361 0.1482531744765928 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.279253472113207E-5 0.0 12-13 0.0 0.0 0.0 6.279253472113207E-5 0.0 14-15 0.0 0.0 0.0 6.279253472113207E-5 0.0 16-17 0.0 0.0 0.0 6.279253472113207E-5 0.0 18-19 0.0 0.0 0.0 6.279253472113207E-5 0.0 20-21 0.0 0.0 0.0 6.279253472113207E-5 0.0 22-23 0.0 0.0 0.0 6.279253472113207E-5 0.0 24-25 0.0 0.0 0.0 1.8837760416339622E-4 0.0 26-27 0.0 0.0 0.0 2.1977387152396225E-4 0.0 28-29 0.0 0.0 0.0 5.337365451296226E-4 0.0 30-31 0.0 0.0 0.0 0.0014756245659466039 0.0 32-33 0.0 0.0 0.0 0.002637286458287547 0.0 34-35 0.0 0.0 0.0 0.003610570746465094 0.0 36-37 0.0 0.0 0.0 0.0049606102429694335 0.0 38-39 0.0 0.0 0.0 0.008508388454713396 0.0 40-41 0.0 0.0 0.0 0.013563187499764527 0.0 42-43 0.0 0.0 0.0 0.017173758246229624 0.0 44-45 0.0 0.0 0.0 0.01934010069410868 0.0 46-47 0.0 0.0 0.0 0.022856482638492076 0.0 48-49 0.0 0.0 0.0 0.02734614887105302 0.0 50-51 0.0 0.0 0.0 0.03431612022509868 0.0 52-53 0.0 0.0 0.0 0.051521274738688866 0.0 54-55 0.0 0.0 0.0 0.06298091232529547 0.0 56-57 0.0 0.0 0.0 0.09368646180392906 0.0 58-59 0.0 0.0 0.0 0.1480020043377083 0.0 60-61 0.0 0.0 0.0 0.1967918038160279 0.0 62-63 0.0 0.0 0.0 0.22746595702730094 0.0 64-65 0.0 0.0 0.0 0.2779511549430911 0.0 66-67 0.0 0.0 0.0 0.3690945190908143 0.0 68-69 0.0 0.0 0.0 0.49430283332475167 0.0 70-71 0.0 0.0 0.0 0.62400081379125 0.0 72-73 0.0 0.0 0.0 0.757843101549343 0.0 74-75 0.0 0.0 0.0 0.9170849696021339 0.0 76-77 0.0 0.0 0.0 1.047536460485286 3.1396267360566035E-5 78-79 0.0 0.0 0.0 1.216448378885131 6.279253472113207E-5 80-81 0.0 0.0 0.0 1.4340559079612143 6.279253472113207E-5 82-83 0.0 0.0 0.0 1.6332652243640058 6.279253472113207E-5 84-85 0.0 0.0 0.0 1.8785642612521083 6.279253472113207E-5 86-87 0.0 0.0 0.0 2.183547602392647 6.279253472113207E-5 88-89 0.0 0.0 0.0 2.561087717403453 6.279253472113207E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9070 0.0 30.770967 1 GGTATCA 6200 0.0 26.456825 1 TCAACGC 10840 0.0 25.473757 4 ATCAACG 10840 0.0 25.47135 3 CAACGCA 11020 0.0 25.100798 5 AACGCAG 11075 0.0 24.964346 6 GTACATG 6680 0.0 24.911617 1 TATCAAC 11095 0.0 24.886715 2 TACATGG 6740 0.0 23.97385 2 GAGTACT 5935 0.0 22.811373 12-13 ACGCAGA 12240 0.0 22.627771 7 ACATGGG 6730 0.0 22.59644 3 CGCAGAG 12280 0.0 22.475986 8 AGTACTT 6200 0.0 21.683132 12-13 GTACTTT 6275 0.0 21.61221 14-15 GCAGAGT 12855 0.0 20.951958 9 AGAGTAC 9695 0.0 20.92187 10-11 GCGTCGA 70 4.854882E-4 20.3568 44-45 GTATAGA 470 0.0 19.220562 1 GTCTAGC 285 4.2284228E-8 18.350931 1 >>END_MODULE