FastQCFastQC Report
Thu 26 May 2016
SRR937153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592546
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90720.5696538749901101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71340.44796194270055617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.32627001041100223No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30540.19176840103833737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.11145674913000943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104950.028.0802021
GGTATCA73500.027.226121
TACACCG1652.4283418E-623.033055
GTACATG67150.023.0053231
ATCAACG130600.022.4797123
TATCAAC131050.022.4750212
TCAACGC131450.022.298214
CAACGCA132300.022.1908575
AGTACTT76700.022.17065412-13
ATACGTC5150.022.1385635
TACATGG68700.022.0586722
AACGCAG133700.021.989886
GAGTACT73850.021.86851712-13
AGAGTAC115300.021.17506210-11
ACATGGG68000.021.0282443
GTACTTT78000.020.64280314-15
TAACGCA1404.8489645E-420.3595734
ACGCAGA147200.019.8453247
TAATACG6000.019.794033
CGCAGAG148750.019.6385338
Produced by FastQC (version 0.11.5)