##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937153_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1592546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.168887429311305 33.0 31.0 34.0 31.0 34.0 2 32.40232935186801 34.0 31.0 34.0 31.0 34.0 3 32.47906999232675 34.0 31.0 34.0 31.0 34.0 4 36.08865552392207 37.0 35.0 37.0 35.0 37.0 5 35.981636323220805 37.0 35.0 37.0 35.0 37.0 6 36.06627061321934 37.0 35.0 37.0 35.0 37.0 7 36.01830151216982 37.0 35.0 37.0 35.0 37.0 8 35.99910018297744 37.0 35.0 37.0 35.0 37.0 9 37.698254241949684 39.0 37.0 39.0 35.0 39.0 10-11 37.62384571623049 39.0 37.0 39.0 35.0 39.0 12-13 37.54109834190033 39.0 37.0 39.0 35.0 39.0 14-15 38.75223039083329 40.0 38.0 41.0 35.0 41.0 16-17 38.73305229487877 40.0 38.0 41.0 35.0 41.0 18-19 38.73585598155407 40.0 38.0 41.0 35.0 41.0 20-21 38.63112745251942 40.0 38.0 41.0 34.0 41.0 22-23 38.507667282452125 40.0 38.0 41.0 34.0 41.0 24-25 38.366117211057016 40.0 38.0 41.0 34.0 41.0 26-27 38.32501384575391 40.0 38.0 41.0 34.0 41.0 28-29 38.23162753226595 40.0 38.0 41.0 34.0 41.0 30-31 38.30065913323696 40.0 38.0 41.0 34.0 41.0 32-33 38.22220582639371 40.0 38.0 41.0 34.0 41.0 34-35 38.10255465148259 40.0 37.5 41.0 34.0 41.0 36-37 37.94074676649842 40.0 37.0 41.0 33.0 41.0 38-39 37.816665264300056 40.0 37.0 41.0 33.0 41.0 40-41 37.65362570374734 40.0 37.0 41.0 33.0 41.0 42-43 37.44092855088644 40.0 37.0 41.0 33.0 41.0 44-45 37.144178252935866 40.0 36.5 41.0 32.0 41.0 46-47 36.7884711022476 40.0 36.0 41.0 31.0 41.0 48-49 36.47405255483986 39.0 36.0 41.0 30.5 41.0 50-51 36.24827257736982 39.0 36.0 41.0 30.0 41.0 52-53 36.01762869016028 39.0 35.0 40.5 29.5 41.0 54-55 35.787001756935126 39.0 35.0 40.0 29.0 41.0 56-57 35.53307220011227 38.5 35.0 40.0 28.0 41.0 58-59 35.28257048775985 38.0 35.0 40.0 28.0 41.0 60-61 34.95148523182375 38.0 34.0 40.0 27.0 41.0 62-63 34.59071920057568 37.5 34.0 40.0 26.0 41.0 64-65 34.23075251829461 37.0 34.0 40.0 26.0 41.0 66-67 33.79318713556783 36.5 33.0 39.0 26.0 40.5 68-69 33.38732130814432 36.0 33.0 39.0 25.0 40.0 70-71 32.96995283024792 35.5 32.5 38.5 24.0 40.0 72-73 32.516736094279224 35.0 32.0 37.5 23.0 40.0 74-75 32.06150120624459 35.0 31.5 37.0 22.0 39.0 76-77 30.75760888539483 34.0 30.0 35.5 20.5 38.5 78-79 31.393429451959314 34.5 31.0 36.0 21.0 38.5 80-81 31.24175125867636 34.5 31.0 36.0 20.0 37.5 82-83 30.91436008755791 34.0 31.0 35.0 19.5 37.0 84-85 30.542749785563494 34.0 31.0 35.0 18.0 36.5 86-87 30.16754429699362 34.0 30.5 35.0 16.0 36.0 88-89 29.762418793554474 34.0 30.0 35.0 9.0 36.0 90-91 29.315497951079593 34.0 29.5 35.0 4.5 35.0 92-93 28.93324776803935 34.0 29.0 35.0 2.0 35.0 94-95 28.499843332625872 34.0 29.0 35.0 2.0 35.0 96-97 27.998653414092907 33.0 29.0 35.0 2.0 35.0 98-99 27.461195469393033 33.0 27.5 35.0 2.0 35.0 100-101 25.525780730980454 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09218351620115328 1101 2 0.02418346818060968 1101 3 -0.0034209611129369932 1101 4 -0.011836962762608039 1101 5 -0.05845597754989029 1101 6 -0.08418803870001312 1101 7 -0.14267558721030582 1101 8 -0.07183860987640855 1101 9 0.06767246937947391 1101 10-11 -0.0720834939115278 1101 12-13 -0.1383881283519912 1101 14-15 -0.21714456591265474 1101 16-17 -0.10584314892883384 1101 18-19 -0.15455986415335587 1101 20-21 -0.05346660829520289 1101 22-23 -0.05780359965323356 1101 24-25 0.03317128042611728 1101 26-27 -0.03401687589299485 1101 28-29 -0.11418886334168121 1101 30-31 -0.07140447934968819 1101 32-33 -0.04281914439465595 1101 34-35 -0.21017118437188032 1101 36-37 -0.21952639510359262 1101 38-39 -0.3049572042380504 1101 40-41 -0.32484504374254897 1101 42-43 -0.13456761243904936 1101 44-45 -0.2172557896208147 1101 46-47 -0.16300120280968144 1101 48-49 -0.07517839126693815 1101 50-51 -0.1032383581161298 1101 52-53 -0.05497508144413388 1101 54-55 -0.03727605693504188 1101 56-57 -0.08928599268282511 1101 58-59 -0.3732691134035804 1101 60-61 -0.41167992096124806 1101 62-63 -0.31737181282902327 1101 64-65 -0.36421674483467115 1101 66-67 -0.28166221955640935 1101 68-69 -0.21628873671376425 1101 70-71 -0.39194837020209405 1101 72-73 -0.34317094469224685 1101 74-75 -0.3823434364802871 1101 76-77 -0.2712400003006543 1101 78-79 -0.3554120089625741 1101 80-81 -0.32051076461001315 1101 82-83 -0.40015336037384586 1101 84-85 -0.3898621802263449 1101 86-87 -0.311145748769885 1101 88-89 -0.38553343240524995 1101 90-91 -0.2644726687611012 1101 92-93 -0.5627056238021773 1101 94-95 -0.6276758706184573 1101 96-97 -0.5882668840411469 1101 98-99 -0.3642762506048314 1101 100-101 -0.04348836065672401 1102 1 0.08917189699795358 1102 2 0.08250995709720144 1102 3 0.12754045002757408 1102 4 0.06557327678818581 1102 5 -0.05381170900000143 1102 6 -0.00976734937434287 1102 7 0.03516806886856472 1102 8 0.06360075617512706 1102 9 0.08830628132902518 1102 10-11 0.06305054669084598 1102 12-13 0.09508152452800545 1102 14-15 0.029167753354926163 1102 16-17 -0.01213724422586182 1102 18-19 0.061516590067164145 1102 20-21 -0.02428106734359403 1102 22-23 -0.011608336975520217 1102 24-25 0.03420454087873992 1102 26-27 -0.09448087046903453 1102 28-29 0.080704557145161 1102 30-31 0.12817712012793692 1102 32-33 0.010609623997105189 1102 34-35 0.012787378277856476 1102 36-37 0.07932215027773282 1102 38-39 0.003373493710448372 1102 40-41 0.03812366608679696 1102 42-43 -0.049681449688293355 1102 44-45 0.010746444578821013 1102 46-47 0.11011889565891408 1102 48-49 0.16255205322958233 1102 50-51 0.1899728147021662 1102 52-53 0.3363135876144696 1102 54-55 0.21482208858824947 1102 56-57 0.2289539039342472 1102 58-59 0.1143645233592423 1102 60-61 0.14320551374073887 1102 62-63 -0.003912921419775728 1102 64-65 0.016917691644209754 1102 66-67 0.15143214693878804 1102 68-69 0.2774444698177376 1102 70-71 0.09589958591477199 1102 72-73 0.11665122939786698 1102 74-75 0.07485412182618134 1102 76-77 0.0680138862283961 1102 78-79 0.1557614918826431 1102 80-81 0.20122615497017193 1102 82-83 0.19117592015471985 1102 84-85 0.1830404136748811 1102 86-87 0.18685666744194052 1102 88-89 0.1552399146396688 1102 90-91 0.2538907686301144 1102 92-93 0.3200496312310257 1102 94-95 0.23620756422459266 1102 96-97 0.12009054694779309 1102 98-99 0.200922563585749 1102 100-101 0.45583091375168294 1103 1 0.024603664970911154 1103 2 0.007690950966477317 1103 3 0.02466695054660306 1103 4 0.07313509417956965 1103 5 -0.08872334140500016 1103 6 -0.036048723863082444 1103 7 -0.030911772495635148 1103 8 -0.0032598430565329295 1103 9 -0.06295069447634916 1103 10-11 -0.09225295074951134 1103 12-13 -0.049466478772465905 1103 14-15 -0.0804984504761066 1103 16-17 -0.037139462287989033 1103 18-19 0.025856916211019154 1103 20-21 -0.026555246438810798 1103 22-23 0.048598186788801456 1103 24-25 -0.039302814215261606 1103 26-27 0.02481083575307963 1103 28-29 -0.19501176601761472 1103 30-31 -0.1230876188948713 1103 32-33 -0.06549519274660298 1103 34-35 0.011823519382097913 1103 36-37 0.025093193033100647 1103 38-39 2.702029934837924E-4 1103 40-41 0.11100530585576251 1103 42-43 0.028277556087537903 1103 44-45 0.02067903767088808 1103 46-47 0.13469446947158303 1103 48-49 0.1061343122814904 1103 50-51 0.10401381336245663 1103 52-53 0.16319605693867345 1103 54-55 0.13785355845365643 1103 56-57 0.032774183232625376 1103 58-59 0.08227714945607545 1103 60-61 0.06753488265770358 1103 62-63 0.01997261326628319 1103 64-65 -0.033398714917701966 1103 66-67 0.21021112592043067 1103 68-69 0.09938077150967928 1103 70-71 -0.07909819602310364 1103 72-73 0.1921942110056989 1103 74-75 0.24339079970145505 1103 76-77 0.10015216465729893 1103 78-79 0.05520475828964777 1103 80-81 0.013272117653496451 1103 82-83 0.09727825266884338 1103 84-85 0.13887535880002133 1103 86-87 0.19760373294575118 1103 88-89 0.13395405966544516 1103 90-91 0.1783780635702712 1103 92-93 0.1769780809830479 1103 94-95 0.3544131963279362 1103 96-97 0.4527838954230994 1103 98-99 0.48300661039573356 1103 100-101 0.8053360640919323 1104 1 0.05128233320469633 1104 2 0.03248008148219128 1104 3 0.04305243840055084 1104 4 -0.018008564756101464 1104 5 -0.03990093977974141 1104 6 -0.07132659471061942 1104 7 -0.07311868695831691 1104 8 -0.08856957921705799 1104 9 -0.08886419632799658 1104 10-11 -0.05513772772777514 1104 12-13 -0.033298488742453 1104 14-15 -0.05261995792258034 1104 16-17 -0.07670408195176748 1104 18-19 -0.1666914534727013 1104 20-21 -0.22702507144992978 1104 22-23 -0.32369470197244965 1104 24-25 -0.24060638915769061 1104 26-27 -0.3285919940994475 1104 28-29 -0.2598252143319968 1104 30-31 -0.16644128575476458 1104 32-33 -0.14360864212462587 1104 34-35 -0.0671735387271184 1104 36-37 -0.14933283246269724 1104 38-39 -0.1769246478555786 1104 40-41 -0.055027932457782924 1104 42-43 -0.0960464693834382 1104 44-45 -0.08306970145546444 1104 46-47 -0.0978785504027968 1104 48-49 0.030614850065155963 1104 50-51 -0.23671834427820926 1104 52-53 -0.045954295928673616 1104 54-55 -0.030974099397084842 1104 56-57 0.058260118175986975 1104 58-59 0.16052399882310908 1104 60-61 0.09761669097654391 1104 62-63 -0.10338415148704883 1104 64-65 -0.11513446150559048 1104 66-67 0.05069077643905473 1104 68-69 0.05034153950360576 1104 70-71 -0.10939528356009731 1104 72-73 -0.08536972791927155 1104 74-75 -0.271418482633198 1104 76-77 -0.2374239309510493 1104 78-79 -0.23167834431931666 1104 80-81 -0.11624453562885151 1104 82-83 -0.03084246501423138 1104 84-85 -0.09988746488330946 1104 86-87 0.04902196096843525 1104 88-89 -0.001266994309698788 1104 90-91 0.12011982866928861 1104 92-93 -0.05398328088458726 1104 94-95 -0.028353696719840116 1104 96-97 0.011321228806068007 1104 98-99 0.2523334323913957 1104 100-101 0.5711315534881827 1105 1 0.0037632015653912276 1105 2 0.06216421073713718 1105 3 -0.07665825942173399 1105 4 -0.05146906388183936 1105 5 -0.09270880589232888 1105 6 -0.03770486752665647 1105 7 0.019785007662115106 1105 8 0.040517324977138 1105 9 0.08008691296645765 1105 10-11 -0.14968322356082808 1105 12-13 -0.11162693981768967 1105 14-15 -0.05302529888586349 1105 16-17 0.07048578111793091 1105 18-19 0.07744643057934297 1105 20-21 -0.0257079971714802 1105 22-23 0.04864219654470503 1105 24-25 -0.08653708380083458 1105 26-27 0.06408007711339536 1105 28-29 -0.0010042228929734165 1105 30-31 0.03268331401360314 1105 32-33 -0.004702722102827295 1105 34-35 -0.06432933953099251 1105 36-37 -0.029183866820169158 1105 38-39 -0.07811682715934865 1105 40-41 -0.0633379899094706 1105 42-43 -0.20255803919972948 1105 44-45 -0.0978107146943259 1105 46-47 -0.3045683794102203 1105 48-49 -0.2643462054109236 1105 50-51 -0.18618356670480551 1105 52-53 -0.1678586036698917 1105 54-55 -0.19163445319048833 1105 56-57 -0.1540624741895229 1105 58-59 -0.22657889192785063 1105 60-61 -0.06487621271807598 1105 62-63 -0.05293680039591919 1105 64-65 -0.1860611822177134 1105 66-67 -0.11055090669104572 1105 68-69 0.03806436349252351 1105 70-71 0.05983740713183039 1105 72-73 -0.03036484566116826 1105 74-75 0.057234695376205735 1105 76-77 5.895577062169366E-4 1105 78-79 -0.015382956350109112 1105 80-81 0.09299542167977393 1105 82-83 0.054899070512711035 1105 84-85 0.18263064488802172 1105 86-87 0.27510343790828173 1105 88-89 0.1958411193220222 1105 90-91 0.1254835001273804 1105 92-93 0.10368413515192643 1105 94-95 0.20668842694889022 1105 96-97 0.12942872931700222 1105 98-99 0.2443719454599531 1105 100-101 0.4919379208610728 1106 1 -0.18179586989318963 1106 2 -0.03183731567930437 1106 3 -0.06862056208095879 1106 4 0.03049864126252544 1106 5 -0.14503144573522775 1106 6 -0.1616052902925631 1106 7 -0.07750946207968212 1106 8 0.015165091521176066 1106 9 -0.13198978517182525 1106 10-11 -0.2467392979264602 1106 12-13 -0.41006579311877545 1106 14-15 -0.21309192953595613 1106 16-17 -0.10835963062861964 1106 18-19 -0.2248817832561656 1106 20-21 -0.16998093415106297 1106 22-23 -0.22754399195914488 1106 24-25 -0.13166284164944386 1106 26-27 -0.20734677924053102 1106 28-29 -0.14749989661717677 1106 30-31 -0.04448346842060147 1106 32-33 -0.13375780172516727 1106 34-35 -0.24803312898842478 1106 36-37 -0.36788538380369573 1106 38-39 -0.2967914174708923 1106 40-41 -0.4308952090874314 1106 42-43 -0.4874186465076633 1106 44-45 -0.39937786415450915 1106 46-47 -0.07744533137955756 1106 48-49 -0.1575330713692651 1106 50-51 -0.24108825143121493 1106 52-53 0.06247334902834467 1106 54-55 -0.1528051631456364 1106 56-57 -0.1806765738182179 1106 58-59 -0.18204998005695927 1106 60-61 -0.05956548406861373 1106 62-63 -0.12162082361803073 1106 64-65 -0.11899233054595015 1106 66-67 -0.22940643737801736 1106 68-69 -0.019340471302911055 1106 70-71 0.18472056767138412 1106 72-73 0.19327445698947798 1106 74-75 0.022344931799242573 1106 76-77 0.006900174875827503 1106 78-79 0.08136011689215294 1106 80-81 0.03553013050297693 1106 82-83 -0.09415549458723405 1106 84-85 -0.0775912000177783 1106 86-87 -0.012600763167068152 1106 88-89 -0.06313472385155094 1106 90-91 -0.07395652799984376 1106 92-93 0.03227871723111164 1106 94-95 -0.7393377450902427 1106 96-97 -0.46911002708236893 1106 98-99 -0.2702542111435129 1106 100-101 -0.06082163424856191 1107 1 0.002629221403282145 1107 2 -0.05207549480691398 1107 3 -0.09906045701574584 1107 4 -0.034181955801891206 1107 5 -0.12847248334398387 1107 6 -0.10374094379291421 1107 7 -0.02907086318864316 1107 8 -0.07037186161537079 1107 9 -0.020719593210891674 1107 10-11 -0.042649298879844366 1107 12-13 -0.026451903619630457 1107 14-15 -0.15465928581396327 1107 16-17 -0.15778117783957413 1107 18-19 -0.1274047852693201 1107 20-21 -0.045359142653829565 1107 22-23 -0.07035129457154454 1107 24-25 -0.15198654861090688 1107 26-27 -0.15756432944149878 1107 28-29 -0.12908308728897566 1107 30-31 -0.17129305914435378 1107 32-33 -0.09082296730235839 1107 34-35 -0.025083206295086313 1107 36-37 -0.17987738094079475 1107 38-39 -0.2631409817487196 1107 40-41 -0.2494859756738279 1107 42-43 -0.09268848751432301 1107 44-45 0.015233440165680179 1107 46-47 -0.03671225145179591 1107 48-49 -0.020024902010334245 1107 50-51 0.04470183093250313 1107 52-53 0.005419789813110754 1107 54-55 -0.12844009332093265 1107 56-57 -0.08606358381211976 1107 58-59 -0.10289859855446082 1107 60-61 0.059565254690141956 1107 62-63 0.008814373503589934 1107 64-65 0.050900075715404114 1107 66-67 0.10428420609802913 1107 68-69 0.023960654376026014 1107 70-71 -0.2556431812387885 1107 72-73 -0.20449643066231715 1107 74-75 -0.12905605280712606 1107 76-77 -0.2745860197711494 1107 78-79 -0.2448556149787784 1107 80-81 -0.19364715805743415 1107 82-83 -0.1256635893338327 1107 84-85 0.06922112649129275 1107 86-87 0.04325388649351325 1107 88-89 -0.03649964306989162 1107 90-91 -0.07565589282786789 1107 92-93 0.01175866931341929 1107 94-95 -0.078141543686705 1107 96-97 -0.0590663788712007 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58-59 -0.9575747065057527 2316 60-61 -0.8687398311339365 2316 62-63 -0.7354429205886106 2316 64-65 -0.8688693635333635 2316 66-67 -0.8185565135772492 2316 68-69 -0.5452018451468206 2316 70-71 -0.7254227997701648 2316 72-73 -0.7868996320466977 2316 74-75 -0.8843845891535267 2316 76-77 -0.9795573269182789 2316 78-79 -0.9293843778142161 2316 80-81 -0.5853252764170449 2316 82-83 -0.8992054070992275 2316 84-85 -1.0874870207520289 2316 86-87 -0.9223853088341976 2316 88-89 -0.8850401884249592 2316 90-91 -0.6299397802958993 2316 92-93 -0.6494786224884415 2316 94-95 -0.4740606056658123 2316 96-97 -0.6602360420641844 2316 98-99 -1.2679158220516946 2316 100-101 -1.8025677255102437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 25.0 6 63.0 7 168.0 8 336.0 9 675.0 10 1177.0 11 1947.0 12 3084.0 13 4556.0 14 5970.0 15 8213.0 16 11198.0 17 16131.0 18 14349.0 19 8457.0 20 7250.0 21 7367.0 22 7539.0 23 8182.0 24 9458.0 25 11203.0 26 13684.0 27 16874.0 28 21300.0 29 27296.0 30 34406.0 31 43249.0 32 56233.0 33 74893.0 34 103787.0 35 150711.0 36 232586.0 37 361187.0 38 299469.0 39 29508.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98276751378869 19.8442799944462 15.250206853916842 21.92274563784827 2 15.73869765771287 21.20372033209716 39.281942248449965 23.775639761740006 3 19.463676402439866 24.95613941449729 30.166287190448504 25.413896992614344 4 13.17619710827819 17.379906137719097 37.25638066341569 32.18751609058702 5 13.674212021067204 37.628294262541154 34.01457571041515 14.682918005976495 6 31.487504913515842 37.096448077480964 17.043024188940226 14.37302282006297 7 28.509254991692544 33.170721599250506 20.355707150688268 17.964316258368676 8 26.649716868460942 34.940278020226735 19.339472768761468 19.07053234255086 9 26.32388640579299 17.092881461508806 20.406129556069338 36.17710257662887 10-11 24.988351984809228 27.21371941532615 26.99394554380219 20.80398305606243 12-13 26.21472832584823 24.34888376485718 27.86550170298701 21.570886206307584 14-15 22.662265328599613 25.68823757681097 26.672479162297353 24.977017932292064 16-17 21.815413809083065 29.34700159367453 26.983145227830153 21.854439369412248 18-19 21.69422421707128 27.34234992270239 30.010624496874815 20.952801363351515 20-21 22.472694666276517 26.25974383157535 30.24926752508248 21.018293977065657 22-23 22.12047962781153 26.166503351749565 30.24615288976353 21.466864130675376 24-25 22.212391686632106 26.460784273710576 30.250162711411022 21.076661328246292 26-27 21.913780837074253 26.766489400781023 30.17196611917065 21.147763642974073 28-29 21.504322001090745 26.93549698695409 30.36794858873658 21.192232423218584 30-31 21.666658294328702 26.482092197022883 30.347223879247444 21.50402562940097 32-33 21.62471288113499 26.683624837210353 30.23397754287788 21.457684738776774 34-35 22.232544617235547 26.56598302341031 29.917503167883375 21.283969191470764 36-37 22.068090968800902 26.39876650344794 30.017688657030945 21.51545387072022 38-39 21.799276127659734 26.796651399708395 30.180698077167 21.223374395464873 40-41 21.962316944063154 26.65951250387744 30.072192577168888 21.30597797489052 42-43 21.866014359753752 26.67931086998504 29.69611520460215 21.758559565659056 44-45 22.0248583086454 26.864561526009297 29.40838129636444 21.702198868980865 46-47 22.146393259598153 27.242164433554823 28.854111592381003 21.75733071446602 48-49 22.40155072443747 27.333935723049755 28.686581109745024 21.577932442767743 50-51 22.10796422834882 27.39443633025357 28.6321399821418 21.86545945925581 52-53 22.521861220963164 27.63037927946822 28.165685637965876 21.682073861602742 54-55 22.10617464110927 27.430416452648775 28.356826113657004 22.106582792584955 56-57 22.28676597096724 27.82390587147875 28.244333287704094 21.644994869849913 58-59 22.593664484416777 27.337326519924698 28.22709673692314 21.841912258735384 60-61 22.373356876347685 27.35092110369182 28.756061049413955 21.519660970546532 62-63 22.177538356819834 27.43694687625978 28.736375589778884 21.649139177141507 64-65 22.112649214732013 27.394256553423435 28.508887187210664 21.984207044633887 66-67 22.069001460554357 27.819635979117713 28.162671596299262 21.948690964028668 68-69 22.065045530866925 27.567649537281813 28.423292011659317 21.944012920191945 70-71 22.101151238331575 27.640457481290966 28.286121719561002 21.972269560816454 72-73 22.33346624428195 27.40497128809547 28.428019302437296 21.833543165185283 74-75 22.075469091630634 27.938408058542734 27.97297534890672 22.01314750091991 76-77 22.1943667561251 27.610034498218578 28.11887380333127 22.076724942325058 78-79 22.153583004823723 27.694584646220584 28.08349021001591 22.068342138939787 80-81 22.031011977048074 28.062548899686412 27.92801589404639 21.978423229219125 82-83 22.175486957941402 27.735347306818035 28.016564733067927 22.07260100217263 84-85 22.008707547409728 27.789741646371347 27.822331370937942 22.379219435280984 86-87 22.110224759598783 27.677630661845875 28.04452744222145 22.167617136333895 88-89 22.058986051266338 27.639986537280553 27.81332532937824 22.48770208207487 90-91 22.057849220758097 27.67013673235237 27.969185766471718 22.302828280417813 92-93 22.15511709070998 27.49572063501632 28.000830127734176 22.348332146539523 94-95 22.016161542586524 27.389381531208517 27.883370401859665 22.711086524345294 96-97 21.973054467500468 27.40398079553118 27.926634458282525 22.696330278685828 98-99 21.86263671918538 27.55237141089082 27.702791163946227 22.882200705977578 100-101 21.5520135097497 27.6097659312775 28.020735631996224 22.817484926976576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 700.0 1 498.5 2 413.0 3 531.5 4 432.0 5 402.5 6 739.0 7 1584.0 8 2472.0 9 2823.0 10 2625.0 11 2273.5 12 2286.5 13 2461.0 14 2598.5 15 2751.0 16 2897.5 17 3032.0 18 3186.5 19 3309.5 20 3494.0 21 3894.5 22 4586.0 23 5454.5 24 6467.0 25 7926.5 26 9927.0 27 12193.5 28 13900.0 29 15682.5 30 18362.5 31 21139.0 32 24129.0 33 27592.0 34 31364.5 35 34650.0 36 38204.5 37 41556.5 38 44462.0 39 45678.0 40 46118.0 41 49233.0 42 52956.0 43 55895.5 44 57465.0 45 58889.0 46 59092.0 47 60808.0 48 68465.5 49 69063.0 50 63902.0 51 60761.0 52 56183.5 53 53572.0 54 51118.0 55 46468.5 56 41086.0 57 35680.5 58 30992.5 59 27184.5 60 23364.5 61 18594.5 62 14429.5 63 11187.5 64 8363.0 65 6391.5 66 4712.0 67 3618.5 68 2535.5 69 1491.5 70 1096.5 71 900.5 72 713.0 73 547.0 74 439.0 75 364.0 76 228.5 77 109.5 78 69.0 79 53.5 80 34.5 81 21.0 82 10.5 83 4.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05293410676991434 2 0.0 3 0.0 4 0.0 5 0.01934010069410868 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031396267360566037 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012244544270620754 24-25 1.569813368028302E-4 26-27 0.0014442282985860377 28-29 0.003422193142301698 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015603944878201319 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.1396267360566035E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.279253472113207E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.7675520832679244E-4 84-85 0.0012244544270620754 86-87 0.0 88-89 0.0 90-91 0.010360768228986792 92-93 0.0012558506944226415 94-95 0.0 96-97 0.0 98-99 1.569813368028302E-4 100-101 0.01346899869768283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1592546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.51393647835664 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28914105657545 42.67849262503257 2 12.544323617121526 13.676809216554235 3 3.800306367206658 6.215089797005941 4 1.704767294369135 3.7173430398247573 5 0.8903433155041071 2.426805947266017 6 0.5733378732049688 1.8752942640319057 7 0.3660864790047382 1.3969770543434676 8 0.2642501949603706 1.1524254673970402 9 0.18813086672461174 0.9230178706419296 >10 1.2007223648257168 12.356117964323724 >50 0.11090292148658815 4.207268895688717 >100 0.06296004872382954 6.078117752868982 >500 0.003689834374548948 1.3356815727146645 >1k 6.918439452279278E-4 0.6152595996861563 >5k 3.459219726139639E-4 1.3452989326198526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9072 0.5696538749901101 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7134 0.44796194270055617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5196 0.32627001041100223 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3054 0.19176840103833737 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1775 0.11145674913000943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.279253472113207E-5 0.0 12-13 0.0 0.0 0.0 6.279253472113207E-5 0.0 14-15 0.0 0.0 0.0 6.279253472113207E-5 0.0 16-17 0.0 0.0 0.0 6.279253472113207E-5 0.0 18-19 0.0 0.0 0.0 6.279253472113207E-5 0.0 20-21 0.0 0.0 0.0 6.279253472113207E-5 0.0 22-23 0.0 0.0 0.0 6.279253472113207E-5 0.0 24-25 0.0 0.0 0.0 1.8837760416339622E-4 0.0 26-27 0.0 0.0 0.0 2.1977387152396225E-4 0.0 28-29 0.0 0.0 0.0 5.337365451296226E-4 0.0 30-31 0.0 0.0 0.0 0.0014756245659466039 0.0 32-33 0.0 0.0 0.0 0.002574493923566415 0.0 34-35 0.0 0.0 0.0 0.003453589409662264 0.0 36-37 0.0 0.0 0.0 0.0048036289061666035 0.0 38-39 0.0 0.0 0.0 0.008414199652631698 0.0 40-41 0.0 0.0 0.0 0.013374809895601133 0.0 42-43 0.0 0.0 0.0 0.016985380642066228 0.0 44-45 0.0 0.0 0.0 0.019214515624666414 0.0 46-47 0.0 0.0 0.0 0.022856482638492076 0.0 48-49 0.0 0.0 0.0 0.027628715277298113 0.0 50-51 0.0 0.0 0.0 0.03466147916606491 0.0 52-53 0.0 0.0 0.0 0.05186663367965509 0.0 54-55 0.0 0.0 0.0 0.06357744140514622 0.0 56-57 0.0 0.0 0.0 0.09450276475530378 0.0 58-59 0.0 0.0 0.0 0.1489124960911647 0.0 60-61 0.0 0.0 0.0 0.1978278806389266 0.0 62-63 0.0 0.0 0.0 0.2287846002564447 0.0 64-65 0.0 0.0 0.0 0.2796151571132011 0.0 66-67 0.0 0.0 0.0 0.3710410876671694 3.1396267360566035E-5 68-69 0.0 0.0 0.0 0.49637498697054905 6.279253472113207E-5 70-71 0.0 0.0 0.0 0.6265439114474558 6.279253472113207E-5 72-73 0.0 0.0 0.0 0.761673446167332 6.279253472113207E-5 74-75 0.0 0.0 0.0 0.9218258059735793 6.279253472113207E-5 76-77 0.0 0.0 0.0 1.053909902759481 6.279253472113207E-5 78-79 0.0 0.0 0.0 1.2242974457252727 6.279253472113207E-5 80-81 0.0 0.0 0.0 1.4444794687249223 6.279253472113207E-5 82-83 0.0 0.0 0.0 1.6451958059610208 6.279253472113207E-5 84-85 0.0 0.0 0.0 1.8938542434567038 6.279253472113207E-5 86-87 0.0 0.0 0.0 2.2036726097707695 6.279253472113207E-5 88-89 0.0 0.0 0.0 2.585702391014137 6.279253472113207E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10495 0.0 28.080202 1 GGTATCA 7350 0.0 27.22612 1 TACACCG 165 2.4283418E-6 23.03305 5 GTACATG 6715 0.0 23.005323 1 ATCAACG 13060 0.0 22.479712 3 TATCAAC 13105 0.0 22.475021 2 TCAACGC 13145 0.0 22.29821 4 CAACGCA 13230 0.0 22.190857 5 AGTACTT 7670 0.0 22.170654 12-13 ATACGTC 515 0.0 22.138563 5 TACATGG 6870 0.0 22.058672 2 AACGCAG 13370 0.0 21.98988 6 GAGTACT 7385 0.0 21.868517 12-13 AGAGTAC 11530 0.0 21.175062 10-11 ACATGGG 6800 0.0 21.028244 3 GTACTTT 7800 0.0 20.642803 14-15 TAACGCA 140 4.8489645E-4 20.359573 4 ACGCAGA 14720 0.0 19.845324 7 TAATACG 600 0.0 19.79403 3 CGCAGAG 14875 0.0 19.638533 8 >>END_MODULE