FastQCFastQC Report
Thu 26 May 2016
SRR937152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763324
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80300.4553899340109929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.3383382747583541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41390.23472714033268985No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25270.14330888707917547No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25210.14296862062785964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104650.028.3339271
GGTATCA72300.025.5665841
GTACATG72600.024.8718681
AATACCG1000.00165219123.7544485
ATCAACG124250.023.743543
TCAACGC125200.023.5640494
TATCAAC125650.023.5167962
TACATGG74000.023.432112
CAACGCA126900.023.2115975
AACGCAG127500.023.1383116
ACATGGG74650.022.5916963
GAGTACT68050.021.63886312-13
AGTACTT70850.020.71664812-13
GTACTTT71550.020.71166414-15
ACGCAGA141250.020.616857
GTATAGG5100.020.4981141
CGCAGAG142100.020.393818
AGAGTAC110050.019.98500310-11
GCAGAGT146500.019.5213189
CATGGGG33000.019.4349084
Produced by FastQC (version 0.11.5)