FastQCFastQC Report
Thu 26 May 2016
SRR937152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763324
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95960.5441994778044194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77020.43678870133906195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55620.3154270003697562No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31840.18056806349825671No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.11291175076162974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117050.028.8305991
GGTATCA81500.028.8095231
ATCAACG140700.023.732563
TATCAAC141850.023.6740972
TCAACGC141550.023.6236044
CAACGCA143550.023.2944725
AACGCAG145800.022.9343386
GTACATG71100.022.8624731
GAGTACT85400.021.80461912-13
TACATGG73000.021.5371632
GTACTTT87900.021.1820614-15
AGTACTT87250.021.09728612-13
ACATGGG71300.020.8515423
ACGCAGA162100.020.5425957
CGCAGAG163150.020.2648078
AGAGTAC127200.020.22235310-11
GCAGAGT170400.018.928699
TACTTTT97500.018.7067214-15
ACTTTTT101900.018.45831516-17
ATACGTC4400.018.351845
Produced by FastQC (version 0.11.5)