##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937152_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1763324 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21786693766999 33.0 31.0 34.0 31.0 34.0 2 32.43547640705849 34.0 31.0 34.0 31.0 34.0 3 32.488208633240404 34.0 31.0 34.0 31.0 34.0 4 36.12487835474366 37.0 35.0 37.0 35.0 37.0 5 36.04603294686626 37.0 35.0 37.0 35.0 37.0 6 36.09092656823136 37.0 35.0 37.0 35.0 37.0 7 36.03271094818649 37.0 35.0 37.0 35.0 37.0 8 36.00607999437426 37.0 35.0 37.0 35.0 37.0 9 37.71848735683289 39.0 37.0 39.0 35.0 39.0 10-11 37.63707407146957 39.0 37.0 39.0 35.0 39.0 12-13 37.54170333982864 39.0 37.0 39.0 35.0 39.0 14-15 38.74705782941763 40.0 38.0 41.0 35.0 41.0 16-17 38.71891155567553 40.0 38.0 41.0 35.0 41.0 18-19 38.70757784729295 40.0 38.0 41.0 34.5 41.0 20-21 38.61585959245153 40.0 38.0 41.0 34.0 41.0 22-23 38.46531578995125 40.0 38.0 41.0 34.0 41.0 24-25 38.409227402337855 40.0 38.0 41.0 34.0 41.0 26-27 38.36830809312412 40.0 38.0 41.0 34.0 41.0 28-29 38.243185597201645 40.0 38.0 41.0 34.0 41.0 30-31 38.30813650809493 40.0 38.0 41.0 34.0 41.0 32-33 38.24885557050207 40.0 38.0 41.0 34.0 41.0 34-35 38.12355018136202 40.0 37.5 41.0 34.0 41.0 36-37 37.9532119451672 40.0 37.0 41.0 33.0 41.0 38-39 37.83275081607237 40.0 37.0 41.0 33.0 41.0 40-41 37.65896199450583 40.0 37.0 41.0 33.0 41.0 42-43 37.45184152203452 40.0 37.0 41.0 33.0 41.0 44-45 37.16546505350123 40.0 36.5 41.0 32.0 41.0 46-47 36.81778561398812 40.0 36.0 41.0 31.0 41.0 48-49 36.496813121128056 39.0 36.0 41.0 30.5 41.0 50-51 36.24790934621204 39.0 36.0 41.0 30.0 41.0 52-53 36.01101385791834 39.0 35.0 40.5 29.5 41.0 54-55 35.78445623152636 39.0 35.0 40.0 29.0 41.0 56-57 35.51263551111424 38.5 35.0 40.0 28.0 41.0 58-59 35.234017117670945 38.0 34.5 40.0 28.0 41.0 60-61 34.904860365990594 38.0 34.0 40.0 27.0 41.0 62-63 34.54491800712745 37.5 34.0 40.0 26.0 41.0 64-65 34.142603117748074 37.0 33.5 39.5 26.0 41.0 66-67 33.720112129137924 36.0 33.0 39.0 25.5 40.5 68-69 33.28498874852268 36.0 33.0 39.0 25.0 40.0 70-71 32.86509144093768 35.0 32.0 38.5 23.5 40.0 72-73 32.38865574335743 35.0 32.0 37.5 22.5 39.5 74-75 31.904377187629727 35.0 31.0 37.0 21.5 39.0 76-77 30.664041038402473 33.5 29.5 35.5 20.0 38.5 78-79 31.26684857689228 34.0 31.0 36.0 20.5 38.5 80-81 31.057157391381278 34.0 31.0 36.0 20.0 37.5 82-83 30.709094301444317 34.0 31.0 35.0 18.5 37.0 84-85 30.347354768607474 34.0 30.5 35.0 17.5 36.5 86-87 29.937831618012346 34.0 30.0 35.0 11.5 36.0 88-89 29.464686864127067 34.0 29.5 35.0 7.0 36.0 90-91 28.94828375273064 34.0 29.0 35.0 2.0 35.0 92-93 28.590610120431638 34.0 29.0 35.0 2.0 35.0 94-95 28.140741860259375 33.5 29.0 35.0 2.0 35.0 96-97 27.646593876111254 33.0 27.5 35.0 2.0 35.0 98-99 26.875081380392942 33.0 26.0 35.0 2.0 35.0 100-101 24.380099743439096 30.5 20.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03285856645680241 1101 2 0.016164179585850036 1101 3 -0.03385125835522729 1101 4 0.02019798395445349 1101 5 -0.008316316062739304 1101 6 -0.10999940219357285 1101 7 -0.08364309990354002 1101 8 -0.0765820552864298 1101 9 -0.017613238818306343 1101 10-11 -0.09300966631600716 1101 12-13 -0.04501436319640817 1101 14-15 -0.0873424922133168 1101 16-17 -0.021310244058639682 1101 18-19 0.030094065873107922 1101 20-21 -0.019738112042851697 1101 22-23 -0.03279538290571793 1101 24-25 -0.026754976125225483 1101 26-27 -0.10134594392955876 1101 28-29 -0.2239290235961775 1101 30-31 -0.1895873224464708 1101 32-33 -0.12236174195197691 1101 34-35 -0.07988373467564713 1101 36-37 -0.07381367966127073 1101 38-39 -0.2108591860786575 1101 40-41 -0.1054482430927024 1101 42-43 -0.1258312745268384 1101 44-45 -0.07789950851396554 1101 46-47 -0.05807175561413658 1101 48-49 -0.14015808175076216 1101 50-51 -0.09751189491078804 1101 52-53 -0.09996123391579204 1101 54-55 -0.14541135453894327 1101 56-57 -0.047156534765441904 1101 58-59 0.09344308107301913 1101 60-61 -0.12876358665739218 1101 62-63 -0.09195675909310808 1101 64-65 -0.15352270078336971 1101 66-67 -0.07690321290699131 1101 68-69 -0.27379984297488136 1101 70-71 -0.26697989727458094 1101 72-73 -0.13955881529867042 1101 74-75 -0.33841924921781086 1101 76-77 -0.18120030364088535 1101 78-79 -0.22669711719291996 1101 80-81 -0.2302578597776197 1101 82-83 -0.0694943345842205 1101 84-85 -0.1648716431324715 1101 86-87 -0.3787831218485991 1101 88-89 -0.3147055912352492 1101 90-91 -0.3533088929076236 1101 92-93 -0.2321923527012686 1101 94-95 -0.1433014406663773 1101 96-97 -0.38084550317422483 1101 98-99 -0.21221911183355502 1101 100-101 0.2656406019752886 1102 1 0.019496796878001987 1102 2 -0.01331095164294993 1102 3 -0.016522258995692596 1102 4 -0.02143467841229807 1102 5 -0.08919602717993769 1102 6 -0.0071703992453819865 1102 7 -0.07115921192542629 1102 8 -0.05900564957716625 1102 9 -0.0669712277846557 1102 10-11 -0.05139093638875636 1102 12-13 -0.09623528820062432 1102 14-15 -0.08295071285925104 1102 16-17 -0.13631841564549774 1102 18-19 -0.09787380761020614 1102 20-21 -0.038390165410014276 1102 22-23 -0.07265123704236487 1102 24-25 -0.14815718749890294 1102 26-27 -0.15113024443454037 1102 28-29 -0.12883590212599216 1102 30-31 -0.17567198185954425 1102 32-33 -0.13898082019022695 1102 34-35 -0.24819309225086528 1102 36-37 -0.29934567719733707 1102 38-39 -0.17126298195069012 1102 40-41 -0.08475445404756954 1102 42-43 -0.1865597173487359 1102 44-45 -0.26948437839131856 1102 46-47 -0.14312950979776673 1102 48-49 -0.13730467592370843 1102 50-51 -0.04965290977501269 1102 52-53 -0.19384159985764882 1102 54-55 -0.12807042816681502 1102 56-57 -0.1521830633968122 1102 58-59 -0.22033350508244354 1102 60-61 -0.141601168610741 1102 62-63 -0.10291451720316047 1102 64-65 -0.180162580575967 1102 66-67 -0.11145471290297593 1102 68-69 -0.14191241539718646 1102 70-71 -0.10477548136677228 1102 72-73 -0.05595014037076851 1102 74-75 -0.06948495840219948 1102 76-77 -0.035935412079592055 1102 78-79 -0.05307960572082138 1102 80-81 -0.14682228485550652 1102 82-83 -0.06685751486196523 1102 84-85 -0.20526790704327524 1102 86-87 -0.28720846909354236 1102 88-89 -0.17948820507066898 1102 90-91 -0.1622385733523508 1102 92-93 -0.16175236626217426 1102 94-95 -0.11703193155688751 1102 96-97 0.1822037463105879 1102 98-99 0.25095787278967663 1102 100-101 0.8886600318176434 1103 1 -0.02657004220684911 1103 2 -0.050831159857793295 1103 3 -0.10406499267609348 1103 4 -0.12285777020235855 1103 5 -0.03552018893487485 1103 6 -0.1209621915591157 1103 7 -0.19355444852066483 1103 8 -0.12132364939157725 1103 9 -0.20833835626019237 1103 10-11 -0.17321961701931343 1103 12-13 -0.17782250341550565 1103 14-15 -0.19408363751477964 1103 16-17 -0.3459521185395502 1103 18-19 -0.3430736529935601 1103 20-21 -0.37434606894419886 1103 22-23 -0.18973452487237097 1103 24-25 -0.1942696877329908 1103 26-27 -0.3418649659930679 1103 28-29 -0.40706012109414047 1103 30-31 -0.4408252234118635 1103 32-33 -0.3470284820132292 1103 34-35 -0.21002794660547153 1103 36-37 -0.3389250559489909 1103 38-39 -0.28170183609572774 1103 40-41 -0.41565985571944 1103 42-43 -0.34915002174026455 1103 44-45 -0.4113010692878518 1103 46-47 -0.21986780555113938 1103 48-49 -0.3565704614774887 1103 50-51 -0.32011683757231424 1103 52-53 -0.2262296632336458 1103 54-55 -0.36067947467154227 1103 56-57 -0.3927750254774409 1103 58-59 -0.5138044447068353 1103 60-61 -0.5171461302185563 1103 62-63 -0.22690757839184528 1103 64-65 -0.5916554918403705 1103 66-67 -0.3793345773672243 1103 68-69 -0.46025752909660156 1103 70-71 -0.3418442754642399 1103 72-73 -0.2006974564105235 1103 74-75 -0.16885897981748954 1103 76-77 -0.0807669803044 1103 78-79 -0.15345659426913372 1103 80-81 -0.2712351340291512 1103 82-83 -0.3189469332799071 1103 84-85 -0.3707097186420043 1103 86-87 -0.3568401435383848 1103 88-89 -0.2600085906696066 1103 90-91 -0.23406124649069326 1103 92-93 -0.10808220935826185 1103 94-95 -0.07698426300043693 1103 96-97 0.062179733175330654 1103 98-99 0.11665191489346682 1103 100-101 0.7033678246607948 1104 1 -0.10071458379270126 1104 2 -0.021773311400444584 1104 3 -0.043284681822214566 1104 4 -0.09919836569401497 1104 5 -0.21692711410513255 1104 6 -0.15875457570257367 1104 7 -0.1490155453005002 1104 8 -0.12263527345247383 1104 9 -0.0520767285234669 1104 10-11 -0.2279208933595669 1104 12-13 -0.11890232326532413 1104 14-15 -0.19457521496336483 1104 16-17 -0.25616351629666667 1104 18-19 -0.21307071847790127 1104 20-21 -0.20636460410688784 1104 22-23 -0.34699671391455666 1104 24-25 -0.2618869285961196 1104 26-27 -0.3415156091393783 1104 28-29 -0.24639313619737635 1104 30-31 -0.3159027075264689 1104 32-33 -0.29755987918058224 1104 34-35 -0.24187532557922964 1104 36-37 -0.3295728999400751 1104 38-39 -0.4597909974529202 1104 40-41 -0.4235844814663068 1104 42-43 -0.2963335522472548 1104 44-45 -0.2022963861448872 1104 46-47 -0.24696456613663997 1104 48-49 -0.211695184536417 1104 50-51 -0.2450324523287719 1104 52-53 -0.21936682839321975 1104 54-55 -0.21230888855271957 1104 56-57 -0.18475656895076753 1104 58-59 -0.25530607302513175 1104 60-61 -0.20140770635038763 1104 62-63 -0.15689664921524127 1104 64-65 -0.21677766946805122 1104 66-67 -0.03904717913583511 1104 68-69 -0.034864978752665365 1104 70-71 -0.12148436827930453 1104 72-73 -0.1284454009696816 1104 74-75 -0.06749398975654941 1104 76-77 -0.06355019946193252 1104 78-79 -0.2356014282826493 1104 80-81 -0.3615756218058621 1104 82-83 -0.49616106425268214 1104 84-85 -0.3295902531375141 1104 86-87 -0.3586691474884134 1104 88-89 -0.3391348463115875 1104 90-91 -0.37253669905219056 1104 92-93 -0.3115342482527801 1104 94-95 -0.1707081469885665 1104 96-97 -0.22095444012983378 1104 98-99 -0.06420015541450041 1104 100-101 0.7179726203515422 1105 1 0.013177186236141836 1105 2 0.013509887226781814 1105 3 -0.0071258627245853745 1105 4 -0.02368358493338718 1105 5 -0.07497816749501851 1105 6 -0.05855242426001439 1105 7 -0.08678271720223307 1105 8 -0.036068193747226474 1105 9 -0.04738405900477716 1105 10-11 -0.08807405106844612 1105 12-13 -0.11553241483431265 1105 14-15 -0.07391010021606803 1105 16-17 -0.17162348772723135 1105 18-19 -0.13936121284871206 1105 20-21 -0.14118634869557667 1105 22-23 -0.14981867258937598 1105 24-25 -0.13953198937894484 1105 26-27 -0.13586568343382055 1105 28-29 -0.151033555576511 1105 30-31 -0.24676949796937464 1105 32-33 -0.10402858179813279 1105 34-35 -0.09214125172115217 1105 36-37 -0.22695756308866777 1105 38-39 -0.28619247210442467 1105 40-41 -0.260327300455657 1105 42-43 -0.12535460856133085 1105 44-45 -0.2720550388951395 1105 46-47 -0.3287662813684591 1105 48-49 -0.1288752272798135 1105 50-51 -0.2630638770555507 1105 52-53 -0.21114225040332002 1105 54-55 -0.05086190419372372 1105 56-57 -0.1671667481310095 1105 58-59 -0.19919940973790773 1105 60-61 -0.20621008475706049 1105 62-63 -0.2810759344546554 1105 64-65 -0.32410977078082226 1105 66-67 -0.18991063935006025 1105 68-69 -0.11383374391453316 1105 70-71 -0.05161959524956927 1105 72-73 -0.13223712469522297 1105 74-75 0.061767277756370476 1105 76-77 0.08396303840211061 1105 78-79 -0.05022785824059994 1105 80-81 -0.1385935373698004 1105 82-83 -0.16435360724729264 1105 84-85 0.006928739214036028 1105 86-87 0.09740279857500411 1105 88-89 0.28047849855450835 1105 90-91 0.2499848541303713 1105 92-93 0.46128376500333346 1105 94-95 0.539339977276537 1105 96-97 0.45832228915907436 1105 98-99 0.5752051855012574 1105 100-101 1.1590049133165152 1106 1 -0.23806752572848566 1106 2 -0.09142941647763081 1106 3 -0.05990801955298508 1106 4 -0.05347041805705999 1106 5 -0.19731291804836815 1106 6 -0.09085787498375453 1106 7 -0.04364641460718133 1106 8 -0.16177481327938636 1106 9 -0.21530074557622214 1106 10-11 -0.2355286283706306 1106 12-13 -0.1205616881242193 1106 14-15 -0.04893491483625212 1106 16-17 -0.11085022700652303 1106 18-19 -0.07934566058407455 1106 20-21 -0.09676154362967537 1106 22-23 -0.04316158675467108 1106 24-25 -0.12097742110025678 1106 26-27 -0.13698764928253127 1106 28-29 -0.23200949999176856 1106 30-31 -0.14898131662889824 1106 32-33 -0.11370362970228598 1106 34-35 -0.06166438637828975 1106 36-37 -0.08806668372222504 1106 38-39 -0.14360992036528586 1106 40-41 -0.06883871518095219 1106 42-43 -0.21816526902832578 1106 44-45 -0.11543165848038228 1106 46-47 -0.19690576872759635 1106 48-49 -0.28445692444059745 1106 50-51 -0.19962204942560646 1106 52-53 -0.07733106975819481 1106 54-55 -0.18298515987189745 1106 56-57 -0.23207224025667728 1106 58-59 -0.18849528493761625 1106 60-61 -0.07632014489602312 1106 62-63 0.03669849932082769 1106 64-65 -0.09116882692291028 1106 66-67 0.038433628339973325 1106 68-69 0.0165657582006844 1106 70-71 0.11134810062669942 1106 72-73 0.02821925199867792 1106 74-75 0.06603486163509942 1106 76-77 0.02201207846113462 1106 78-79 0.06457160778062487 1106 80-81 -0.0013560813593507248 1106 82-83 -0.11608120906685215 1106 84-85 -0.06007405276248434 1106 86-87 6.71191321259812E-5 1106 88-89 0.04602744572457951 1106 90-91 0.11027124404104072 1106 92-93 0.19671944073065717 1106 94-95 0.25292750786353935 1106 96-97 0.2531465167210847 1106 98-99 0.4885285133674131 1106 100-101 1.0738954105112697 1107 1 -0.09067693943616462 1107 2 -0.14528848611704603 1107 3 -0.011873337472060541 1107 4 -0.0018877040465952177 1107 5 -0.04712299236711459 1107 6 -0.048548488801536394 1107 7 -0.08649805237211439 1107 8 -0.0482130186193217 1107 9 -0.06163435416416263 1107 10-11 -0.0858052592542009 1107 12-13 -0.15352205146030684 1107 14-15 -0.11102409733005913 1107 16-17 -0.10210951684963021 1107 18-19 -0.06735281720394681 1107 20-21 -0.10568392182951669 1107 22-23 0.02754719386739879 1107 24-25 0.007827973890115913 1107 26-27 -0.017344104003157668 1107 28-29 -0.07472763927059844 1107 30-31 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0.3720553367403667 2316 22-23 0.35307224427844375 2316 24-25 0.45866945054591923 2316 26-27 0.6148708797102032 2316 28-29 0.7510409352601926 2316 30-31 0.42553243758397485 2316 32-33 0.18944135595667433 2316 34-35 0.06959530244951395 2316 36-37 0.0654004260416059 2316 38-39 0.27871706149032605 2316 40-41 -0.09670409774450661 2316 42-43 0.020928327401790625 2316 44-45 0.07208520870971569 2316 46-47 0.17945929688969642 2316 48-49 -0.1432236268814009 2316 50-51 -0.04838376231621311 2316 52-53 0.05212186842786792 2316 54-55 -0.03334964808211538 2316 56-57 -0.09589360808380576 2316 58-59 0.14944396807337057 2316 60-61 -0.00725802342714843 2316 62-63 0.026300465015317798 2316 64-65 -0.038589480522603026 2316 66-67 0.14096025496471754 2316 68-69 0.35664989017585214 2316 70-71 0.33668826870236757 2316 72-73 0.4008476466187503 2316 74-75 0.47193947649831003 2316 76-77 0.133871360593524 2316 78-79 0.13402285140247372 2316 80-81 0.22331096815330298 2316 82-83 0.3374492899509285 2316 84-85 0.31528996473545945 2316 86-87 0.07912481590555132 2316 88-89 0.35586517551062613 2316 90-91 0.22523408915159493 2316 92-93 0.44128663647792976 2316 94-95 0.46048808853421264 2316 96-97 0.7626298001589156 2316 98-99 0.741450852117076 2316 100-101 0.10438109144365626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 30.0 6 73.0 7 156.0 8 372.0 9 749.0 10 1310.0 11 2134.0 12 3401.0 13 4962.0 14 6423.0 15 8924.0 16 12212.0 17 17188.0 18 15645.0 19 9619.0 20 8310.0 21 8574.0 22 8846.0 23 9728.0 24 11280.0 25 13310.0 26 16249.0 27 20368.0 28 25523.0 29 31871.0 30 39806.0 31 50489.0 32 64653.0 33 85824.0 34 117905.0 35 170423.0 36 260462.0 37 393287.0 38 314127.0 39 29069.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.890527285892965 19.818563169208627 15.246209756684426 22.044699788213983 2 15.80866590598211 21.17727655269253 39.20844949651908 23.805608044806288 3 19.41089669283694 24.84506534250087 30.116132939834085 25.627905024828106 4 13.030560464214178 17.38370259804778 37.231955102975974 32.353781834762074 5 13.73212777770531 37.627865326059776 33.96316298990348 14.676843906331435 6 31.547236922993164 37.06261583237114 17.053474007045786 14.336673237589913 7 28.60206065362917 33.15414523933208 20.279029832294007 17.964764274744745 8 26.681653513477954 34.903852043073194 19.352540996436275 19.061953447012574 9 26.2977762453185 17.06441924456311 20.393813048537876 36.243991461580514 10-11 24.986765049067856 27.206919206827333 26.953874317446573 20.85244142665824 12-13 26.153217495954838 24.280988351418628 27.902716565554254 21.66307758707228 14-15 22.71301246963122 25.629039246332496 26.574583003463914 25.083365280572373 16-17 21.91551297435979 29.30031576726682 26.92244873885911 21.86172251951428 18-19 21.699727333150346 27.37012596664028 29.834222184918936 21.09592451529044 20-21 22.502132336428247 26.27188763948089 30.133117906862267 21.0928621172286 22-23 22.18825514983477 26.213074395922664 30.051596175145708 21.547074279096854 24-25 22.307702341281274 26.48751664485161 30.030346371705907 21.17443464216121 26-27 21.95875727393316 26.76878292445431 29.98885055835111 21.283609243261424 28-29 21.604881383769538 26.93472158696014 30.20619921900809 21.254197810262237 30-31 21.731496607107566 26.450808518143486 30.22383647650722 21.593858398241725 32-33 21.636295995517557 26.70808087453015 30.087720691149215 21.56790243880308 34-35 22.29627113338218 26.568004518738476 29.738975934088117 21.396748413791226 36-37 22.148289862864072 26.34621497416668 29.874467163852803 21.63102799911644 38-39 21.87867383725975 26.818267658662982 30.015459535544736 21.287598968532528 40-41 22.013528438501748 26.65042838164885 29.946620655041833 21.38942252480757 42-43 21.91454033704599 26.693049933002005 29.543491977965104 21.8489177519869 44-45 22.14735089432075 26.85636170148212 29.199581585093814 21.796705819103323 46-47 22.24276423391277 27.18317790717985 28.758923487685756 21.815134371221625 48-49 22.462639441940176 27.285123801730087 28.573458018338666 21.678778737991074 50-51 22.170741168384257 27.352772377623168 28.53375216352752 21.942734290465054 52-53 22.559416919243688 27.548376687544856 28.123540575509825 21.76866581770163 54-55 22.14451229609533 27.347243047789288 28.272852861981125 22.23539179413426 56-57 22.346517146026482 27.762169629631313 28.079411384408083 21.811901839934126 58-59 22.61873030707913 27.30184583207624 28.10833403276993 21.971089828074707 60-61 22.415506169030763 27.288802284775798 28.67348825286788 21.62220329332556 62-63 22.262868310078012 27.35538108708326 28.638696008220837 21.74305459461789 64-65 22.166238923260536 27.377676302731746 28.420791760558966 22.03529301344875 66-67 22.066336078905522 27.759617631246442 28.073740276886156 22.100306012961884 68-69 22.07549491755344 27.49826464109829 28.37921448355492 22.04702595779335 70-71 22.195892530244016 27.54522141137987 28.19907175312081 22.0598143052553 72-73 22.33440933146716 27.37565529647416 28.31090599345327 21.979029378605404 74-75 22.144597362708158 27.84760486444919 27.87499631378011 22.13280145906254 76-77 22.252660316538535 27.5535295838995 28.027435683969593 22.16637441559237 78-79 22.194559819976362 27.62637496001869 28.020913910319372 22.15815130968557 80-81 22.114921656440707 27.971052382348553 27.862025278409003 22.052000682801733 82-83 22.248838763589816 27.69956805776848 27.888360244301285 22.163232934340414 84-85 22.069026139648702 27.70632628553848 27.684464085452564 22.540183489360256 86-87 22.161837529574825 27.609021371001585 27.913503133854018 22.31563796556957 88-89 22.17624214268053 27.539096615256188 27.72060041149556 22.564060830567723 90-91 22.079350270389835 27.624652973721002 27.914184194034124 22.381812561855043 92-93 22.26175393385743 27.35180759515494 27.97054016317299 22.415898307814633 94-95 22.00962500368622 27.311883692390055 27.875308224693818 22.803183079229907 96-97 22.0739070074473 27.33020136968589 27.86209454416772 22.733797078699094 98-99 21.926880059342537 27.451140517727335 27.591387165815433 23.030592257114694 100-101 21.760918218147708 27.427581481821782 27.89912282194295 22.912377478087564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 669.0 1 468.0 2 371.0 3 517.5 4 435.0 5 372.5 6 709.0 7 1586.5 8 2602.0 9 3088.5 10 2797.0 11 2383.0 12 2367.0 13 2562.5 14 2809.0 15 2970.0 16 3065.5 17 3091.5 18 3211.0 19 3389.0 20 3648.5 21 4218.0 22 4887.0 23 5737.5 24 6964.5 25 8417.0 26 10488.0 27 12969.5 28 15031.5 29 17124.0 30 19976.5 31 22891.5 32 26275.5 33 30338.0 34 34738.0 35 38264.5 36 41857.0 37 45935.5 38 49147.0 39 50178.0 40 50830.0 41 54583.0 42 58655.5 43 62017.5 44 64014.0 45 65405.5 46 65317.0 47 67596.5 48 76569.0 49 76913.0 50 71213.0 51 67988.0 52 62626.5 53 59517.5 54 57016.5 55 51801.5 56 46006.0 57 40104.5 58 34842.5 59 30498.0 60 25968.5 61 20782.0 62 16305.5 63 12661.5 64 9475.0 65 7191.5 66 5218.5 67 4077.0 68 2904.0 69 1760.5 70 1294.0 71 980.0 72 762.0 73 629.0 74 518.0 75 404.0 76 268.0 77 159.0 78 88.0 79 57.0 80 34.0 81 20.0 82 15.0 83 9.0 84 4.0 85 2.5 86 2.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06623853585614442 2 0.0 3 0.0 4 0.0 5 0.002438576234429974 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.8355537609650864E-5 12-13 0.005954662898026681 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.50666128289526E-4 24-25 9.073772035088276E-4 26-27 3.969775265351121E-4 28-29 0.0030340425242326424 30-31 3.119109137061595E-4 32-33 0.0 34-35 0.0 36-37 2.8355537609650864E-5 38-39 6.23821827412319E-4 40-41 8.50666128289526E-5 42-43 0.011994392408882315 44-45 1.4177768804825433E-4 46-47 0.0 48-49 2.8355537609650864E-5 50-51 0.0 52-53 2.8355537609650864E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.8355537609650864E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.671107521930173E-5 82-83 5.387552145833664E-4 84-85 3.6862198892546123E-4 86-87 0.0 88-89 0.0 90-91 0.007315728703289923 92-93 0.0023818651592106724 94-95 0.0 96-97 0.0 98-99 1.1342215043860345E-4 100-101 0.009640882787281294 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1763324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.476388098513205 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7218943292671 41.56286184903521 2 12.805026517418572 13.69533135314457 3 3.8780541496551244 6.221529863220213 4 1.7442061156939088 3.730953726665908 5 0.9485742146635369 2.5363161421794835 6 0.5948899470469056 1.9087539410510577 7 0.38743144958388254 1.450290419166208 8 0.27768675307732515 1.187974766190324 9 0.2103477787537381 1.0123775507065582 >10 1.2443114874376744 12.545983393339288 >50 0.11411027889881588 4.248265902865573 >100 0.06815911327670617 6.424070808159851 >500 0.004140134876783455 1.4564110595428206 >1k 8.492584362632728E-4 0.7211377777966265 >5k 3.184719135987273E-4 1.2977414469362263 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9596 0.5441994778044194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7702 0.43678870133906195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5562 0.3154270003697562 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3184 0.18056806349825671 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1991 0.11291175076162974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 1.4177768804825433E-4 0.0 28-29 0.0 0.0 0.0 7.088884402412716E-4 0.0 30-31 0.0 0.0 0.0 0.0017863988694080046 0.0 32-33 0.0 0.0 0.0 0.0032325312875001984 0.0 34-35 0.0 0.0 0.0 0.004707019243202043 0.0 36-37 0.0 0.0 0.0 0.006691906875877604 0.0 38-39 0.0 0.0 0.0 0.010066215851426058 0.0 40-41 0.0 0.0 0.0 0.01409270219199648 0.0 42-43 0.0 0.0 0.0 0.018828076972808173 0.0 44-45 0.0 0.0 0.0 0.020898031218312685 0.0 46-47 0.0 0.0 0.0 0.02557669492390508 0.0 48-49 0.0 0.0 0.0 0.03303420131524326 0.0 50-51 0.0 0.0 0.0 0.04236317318881839 0.0 52-53 0.0 0.0 0.0 0.06110618354879761 0.0 54-55 0.0 0.0 0.0 0.07633310724518012 0.0 56-57 0.0 0.0 0.0 0.1084315758193049 0.0 58-59 0.0 0.0 0.0 0.1594431879790668 0.0 60-61 0.0 0.0 0.0 0.20903702325834617 0.0 62-63 0.0 0.0 0.0 0.24351735699168162 0.0 64-65 0.0 0.0 0.0 0.2964571457088998 0.0 66-67 0.0 0.0 0.0 0.3840474013851113 0.0 68-69 0.0 0.0 0.0 0.505494168967246 0.0 70-71 0.0 0.0 0.0 0.6325553329960916 0.0 72-73 0.0 0.0 0.0 0.7658547152990602 0.0 74-75 0.0 0.0 0.0 0.9233413711830611 0.0 76-77 0.0 0.0 0.0 1.0613477727292318 0.0 78-79 0.0 0.0 0.0 1.2341180633848345 0.0 80-81 0.0 0.0 0.0 1.451491614700418 0.0 82-83 0.0 0.0 0.0 1.6580900617243342 0.0 84-85 0.0 0.0 0.0 1.8953975559795024 0.0 86-87 0.0 0.0 0.0 2.1954842105024373 0.0 88-89 0.0 0.0 0.0 2.5554861159945648 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11705 0.0 28.830599 1 GGTATCA 8150 0.0 28.809523 1 ATCAACG 14070 0.0 23.73256 3 TATCAAC 14185 0.0 23.674097 2 TCAACGC 14155 0.0 23.623604 4 CAACGCA 14355 0.0 23.294472 5 AACGCAG 14580 0.0 22.934338 6 GTACATG 7110 0.0 22.862473 1 GAGTACT 8540 0.0 21.804619 12-13 TACATGG 7300 0.0 21.537163 2 GTACTTT 8790 0.0 21.18206 14-15 AGTACTT 8725 0.0 21.097286 12-13 ACATGGG 7130 0.0 20.851542 3 ACGCAGA 16210 0.0 20.542595 7 CGCAGAG 16315 0.0 20.264807 8 AGAGTAC 12720 0.0 20.222353 10-11 GCAGAGT 17040 0.0 18.92869 9 TACTTTT 9750 0.0 18.70672 14-15 ACTTTTT 10190 0.0 18.458315 16-17 ATACGTC 440 0.0 18.35184 5 >>END_MODULE