FastQCFastQC Report
Thu 26 May 2016
SRR937151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865117
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52910.6115935763601917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.4400560848994991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.2973008275181276No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15220.17592996091858096No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.1123547450807232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.049.334961
GTATCAA42500.043.2919921
TCAACGC55350.033.1420174
ATCAACG55350.033.1381763
TATCAAC55900.032.8971332
CAACGCA56100.032.698945
AACGCAG57850.031.7005926
ACGCAGA66050.027.4053637
ATAGTAC1754.9367372E-927.153193
CGCAGAG66500.027.0770268
GTAGGAC1606.214941E-826.7289223
GTACATG42450.025.1994081
GCAGAGT71200.024.5556379
TACATGG42950.024.4505162
ACATGGG42250.023.6184543
GTATAGG2252.7812348E-923.2431871
GAGTACT39950.022.70841212-13
GTGGTAT12400.022.621211
TGGTATC11600.022.5301252
CATGGGA25750.022.3314744
Produced by FastQC (version 0.11.5)