##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 865117 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.881158271077783 33.0 31.0 34.0 30.0 34.0 2 32.00720711764998 33.0 31.0 34.0 30.0 34.0 3 32.033691396655016 34.0 31.0 34.0 30.0 34.0 4 35.650432253672044 37.0 35.0 37.0 35.0 37.0 5 35.65133502173695 37.0 35.0 37.0 35.0 37.0 6 35.69622490368355 37.0 35.0 37.0 35.0 37.0 7 35.634848234400664 37.0 35.0 37.0 33.0 37.0 8 35.614409380465304 37.0 35.0 37.0 33.0 37.0 9 37.252883713994756 39.0 37.0 39.0 34.0 39.0 10-11 37.14309509580785 39.0 37.0 39.0 34.0 39.0 12-13 37.00887683400049 39.0 37.0 39.0 33.0 39.0 14-15 38.16151745948814 40.0 38.0 41.0 33.0 41.0 16-17 38.09231236930958 40.0 38.0 41.0 33.0 41.0 18-19 38.04430383404788 40.0 38.0 41.0 33.5 41.0 20-21 37.930408834874356 40.0 38.0 41.0 33.0 41.0 22-23 37.97935076989586 40.0 38.0 41.0 33.0 41.0 24-25 37.94308746678195 40.0 38.0 41.0 33.0 41.0 26-27 37.86424784162142 40.0 38.0 41.0 33.0 41.0 28-29 37.70582187149253 40.0 37.5 41.0 33.0 41.0 30-31 37.43886607245031 40.0 37.0 41.0 32.5 41.0 32-33 37.166891877052464 39.5 36.5 41.0 32.0 41.0 34-35 37.191742850966975 40.0 37.0 41.0 32.0 41.0 36-37 37.053433812998705 40.0 36.0 41.0 31.5 41.0 38-39 36.90009443809335 40.0 36.0 41.0 31.0 41.0 40-41 36.66675374544715 39.5 36.0 41.0 30.5 41.0 42-43 36.38909881553593 39.0 35.5 41.0 30.0 41.0 44-45 36.104054711674834 39.0 35.0 41.0 29.5 41.0 46-47 35.636628340444126 39.0 35.0 40.5 27.5 41.0 48-49 35.26335628591278 39.0 35.0 40.0 26.5 41.0 50-51 34.27047671008661 38.0 34.0 39.5 24.5 40.5 52-53 34.34722182086354 38.0 34.0 39.5 24.0 40.5 54-55 34.636872816046846 38.0 34.0 40.0 24.0 41.0 56-57 34.36764391406018 38.0 34.0 40.0 23.0 41.0 58-59 34.10382468498481 38.0 34.0 40.0 22.5 41.0 60-61 33.767407761031166 38.0 33.5 40.0 21.5 41.0 62-63 33.37949491224886 37.0 33.0 40.0 19.5 41.0 64-65 32.94159402716627 36.5 32.5 39.5 18.0 41.0 66-67 32.5077243887243 36.0 32.0 39.0 15.0 40.5 68-69 31.993055852560985 35.5 31.0 39.0 9.5 40.0 70-71 31.53047391277712 35.0 31.0 38.0 7.5 40.0 72-73 31.052366327329135 35.0 31.0 37.5 4.5 39.5 74-75 30.506450572581514 35.0 30.0 37.0 2.0 39.0 76-77 29.99562255741131 34.0 29.5 36.0 2.0 39.0 78-79 29.47355386612447 34.0 29.0 36.0 2.0 38.5 80-81 28.91873873707256 34.0 29.0 35.0 2.0 37.0 82-83 28.38875724323993 34.0 27.5 35.0 2.0 37.0 84-85 27.845423220211835 33.5 26.5 35.0 2.0 36.0 86-87 27.261966878468463 33.0 26.0 35.0 2.0 36.0 88-89 26.720073701013852 33.0 25.0 35.0 2.0 35.5 90-91 26.165257994005437 32.0 24.0 35.0 2.0 35.0 92-93 25.497190553416473 32.0 21.5 34.5 2.0 35.0 94-95 24.72206302731307 31.5 17.5 34.0 2.0 35.0 96-97 23.865896751537655 31.0 4.5 34.0 2.0 35.0 98-99 22.683466513777905 31.0 2.0 34.0 2.0 35.0 100-101 19.6160103199914 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16077625396347273 1101 2 0.2190703527800153 1101 3 0.19376505525490728 1101 4 -0.0026050613803647593 1101 5 0.0955949833839469 1101 6 0.19404408637652892 1101 7 0.13797701858087663 1101 8 0.17761619169801435 1101 9 0.17444901810647195 1101 10-11 0.247049898439883 1101 12-13 0.30017191393863385 1101 14-15 0.43866850276639724 1101 16-17 0.464961752751627 1101 18-19 0.3759814914360149 1101 20-21 0.49679677658134125 1101 22-23 0.5185298960995439 1101 24-25 0.4519973700752047 1101 26-27 0.3429002828175598 1101 28-29 0.34629805363150723 1101 30-31 0.33373060741129024 1101 32-33 0.2954591124004651 1101 34-35 0.28424994285693117 1101 36-37 0.39563096959808064 1101 38-39 0.3417796842382117 1101 40-41 0.5107345963852268 1101 42-43 0.6015129474150527 1101 44-45 0.5142969395680979 1101 46-47 0.6792225821052185 1101 48-49 0.774675709997716 1101 50-51 0.7091273701123484 1101 52-53 0.8031698676798413 1101 54-55 0.8921960770663233 1101 56-57 1.0356780843140072 1101 58-59 0.8472642656561078 1101 60-61 0.9491902595129176 1101 62-63 1.0202533984616693 1101 64-65 1.049657073433984 1101 66-67 1.2993565576047317 1101 68-69 1.1435986870764197 1101 70-71 1.1217261492314314 1101 72-73 1.0243332296076844 1101 74-75 1.2990712632582166 1101 76-77 1.2905391144888831 1101 78-79 1.3592837721966795 1101 80-81 1.4594111071355904 1101 82-83 1.2186209438393618 1101 84-85 1.3071455129827605 1101 86-87 1.4513322054103277 1101 88-89 1.5232254487402521 1101 90-91 1.3552211955364193 1101 92-93 1.542682832429449 1101 94-95 1.631381279014274 1101 96-97 1.6636941951459825 1101 98-99 1.755701401634962 1101 100-101 1.4229865492713394 1102 1 0.2203657382099138 1102 2 0.1908858855915767 1102 3 0.0533249990638609 1102 4 0.10433228559936225 1102 5 0.04964547505563388 1102 6 0.24729200931451345 1102 7 0.062488036040235784 1102 8 0.030117255314308977 1102 9 0.12062923082973498 1102 10-11 0.16404383783382315 1102 12-13 0.2820409887931419 1102 14-15 0.38948222943707833 1102 16-17 0.3720026518084154 1102 18-19 0.4096547003463087 1102 20-21 0.4132394015060896 1102 22-23 0.2961207449857497 1102 24-25 0.2778499087065498 1102 26-27 0.25998188024882296 1102 28-29 0.5066862535110985 1102 30-31 0.5745778078929291 1102 32-33 0.3846860038313267 1102 34-35 0.4558981868866354 1102 36-37 0.5684373914323189 1102 38-39 0.5675688072564711 1102 40-41 0.6777305426023688 1102 42-43 0.852601689136911 1102 44-45 0.8412766706537909 1102 46-47 0.7318133908549669 1102 48-49 0.8479215458395757 1102 50-51 0.5898652388661176 1102 52-53 0.7998545957552992 1102 54-55 0.8663674597675026 1102 56-57 0.8578093305512056 1102 58-59 0.8964148978054212 1102 60-61 0.9157597959367578 1102 62-63 0.8793368421702823 1102 64-65 0.8240386106605584 1102 66-67 0.992942108477699 1102 68-69 1.0337782576162482 1102 70-71 1.035581898779892 1102 72-73 1.0685809518633533 1102 74-75 1.0468917729913123 1102 76-77 0.8449553296203298 1102 78-79 0.8871265978037464 1102 80-81 1.0866016349300907 1102 82-83 1.1250066552581863 1102 84-85 1.044874752396769 1102 86-87 0.9728975275054523 1102 88-89 1.2539285593162113 1102 90-91 1.0871619541155901 1102 92-93 1.265239364638962 1102 94-95 1.1378040765258142 1102 96-97 0.9461264648630241 1102 98-99 1.07380278634297 1102 100-101 0.9840928707644174 1103 1 0.21398012671589584 1103 2 0.13782664600270067 1103 3 0.21493130698381435 1103 4 0.21514431621299224 1103 5 0.18002319915158438 1103 6 0.21391462579560994 1103 7 0.18014907943841507 1103 8 0.10693403402166979 1103 9 0.193530622532478 1103 10-11 0.09947700770682388 1103 12-13 0.15668864976159824 1103 14-15 0.04212465767649576 1103 16-17 0.11064474030349203 1103 18-19 2.5756335855220414E-4 1103 20-21 -0.07527569921395383 1103 22-23 0.0351843912586034 1103 24-25 0.0774042857045174 1103 26-27 0.08680844326015347 1103 28-29 -0.03109281773778605 1103 30-31 -0.024997470043750525 1103 32-33 0.22650420230365143 1103 34-35 0.2721741475595607 1103 36-37 0.29110316876820264 1103 38-39 0.36262781701829283 1103 40-41 0.3459556198983691 1103 42-43 0.28191571366678403 1103 44-45 0.4234117390148455 1103 46-47 0.3858062612200186 1103 48-49 0.35974348316506877 1103 50-51 0.527551713127572 1103 52-53 0.40086004748620496 1103 54-55 0.622384182183886 1103 56-57 0.702455401049825 1103 58-59 0.6137865339825268 1103 60-61 0.45976563987252916 1103 62-63 0.5267496640218354 1103 64-65 0.6012327304187721 1103 66-67 0.7540464607859221 1103 68-69 0.7040534588606491 1103 70-71 0.8904525669354406 1103 72-73 0.7740958852093449 1103 74-75 0.6854164914739869 1103 76-77 0.7540052279052034 1103 78-79 0.7478934540777331 1103 80-81 0.6025679536086201 1103 82-83 0.7080676934935788 1103 84-85 0.5388174355277151 1103 86-87 0.5817056493937329 1103 88-89 0.782079667274143 1103 90-91 0.49295950812286904 1103 92-93 0.773976331737888 1103 94-95 0.6177558294983676 1103 96-97 0.741716325159814 1103 98-99 0.6835370862822678 1103 100-101 0.7943929116503163 1104 1 0.05054038898457236 1104 2 0.163607061640775 1104 3 -0.03519781394340171 1104 4 0.16168059262807333 1104 5 0.13948907621094975 1104 6 0.1957807952372832 1104 7 0.09638461351758565 1104 8 0.11881534781615954 1104 9 0.2685903683174473 1104 10-11 0.24851593219516133 1104 12-13 0.26241516288379074 1104 14-15 0.2500438192220926 1104 16-17 0.249997406760059 1104 18-19 0.2804346559929769 1104 20-21 0.15340183987247968 1104 22-23 0.22386344040334194 1104 24-25 0.11788260214271418 1104 26-27 0.13268467100321146 1104 28-29 0.11705374983404226 1104 30-31 0.19521225298091593 1104 32-33 0.15728991408823134 1104 34-35 0.24177547028309476 1104 36-37 0.09903961938710637 1104 38-39 0.3245995154618413 1104 40-41 0.2983926132628696 1104 42-43 0.22774839045303708 1104 44-45 0.35716613788033413 1104 46-47 0.6512531728225142 1104 48-49 0.6265259209681844 1104 50-51 0.48576850935285876 1104 52-53 0.5365612178908137 1104 54-55 0.7782730812857253 1104 56-57 0.7346021349469112 1104 58-59 0.6498123669219282 1104 60-61 0.7566491202724137 1104 62-63 0.7486888414996429 1104 64-65 0.7721034869360963 1104 66-67 0.7807920006427054 1104 68-69 0.7387661976249404 1104 70-71 0.8998160226491514 1104 72-73 0.990191246485324 1104 74-75 0.8909463687434531 1104 76-77 0.9663418571049185 1104 78-79 0.7779808186101853 1104 80-81 0.9973672252790493 1104 82-83 0.9227295936283895 1104 84-85 0.9709206184679964 1104 86-87 0.9910315724989367 1104 88-89 0.8467714403014348 1104 90-91 0.7861756259161687 1104 92-93 0.8289594147079313 1104 94-95 0.7272766376640618 1104 96-97 0.5670802710953531 1104 98-99 0.500680515559015 1104 100-101 0.40849477711944004 1105 1 0.006944609185488559 1105 2 -0.13770863408677414 1105 3 -0.09333838439487252 1105 4 -0.03228210953546551 1105 5 0.018222125347676865 1105 6 0.19324558731461394 1105 7 0.11167007674222873 1105 8 0.12050449688875631 1105 9 0.09986990616150848 1105 10-11 0.15538785966565172 1105 12-13 0.15626372131824695 1105 14-15 0.14267375604970312 1105 16-17 0.0863466182925876 1105 18-19 0.038464793499805694 1105 20-21 0.18430822123425372 1105 22-23 0.2308285827787202 1105 24-25 0.13372319871810134 1105 26-27 0.17800522340542813 1105 28-29 -0.00275560490507587 1105 30-31 0.12688557926870914 1105 32-33 0.17162096355743728 1105 34-35 0.29433724742228407 1105 36-37 0.4781845655955763 1105 38-39 0.392080872480868 1105 40-41 0.2363208377861028 1105 42-43 0.32777035829685275 1105 44-45 0.4559917488238838 1105 46-47 0.5003170161264805 1105 48-49 0.4800553222553745 1105 50-51 0.31653224816113124 1105 52-53 0.3562905681176147 1105 54-55 0.37073766555912613 1105 56-57 0.6499932813121276 1105 58-59 0.47226914370488515 1105 60-61 0.5191077079181738 1105 62-63 0.40945189752358147 1105 64-65 0.5361421265922104 1105 66-67 0.6214331054471316 1105 68-69 0.460693371254024 1105 70-71 0.6540210835653895 1105 72-73 0.7107179700704762 1105 74-75 0.5743363039086269 1105 76-77 0.6706316723375387 1105 78-79 0.5591039097826709 1105 80-81 0.5884862791180794 1105 82-83 0.5182206936830127 1105 84-85 0.6061163697682552 1105 86-87 0.8269607232339666 1105 88-89 1.016790677007922 1105 90-91 0.7068924900955231 1105 92-93 0.9644824571949293 1105 94-95 1.0553990401149598 1105 96-97 0.8482345328570524 1105 98-99 0.9220601258375858 1105 100-101 1.1905715398904775 1106 1 -0.1704082421605584 1106 2 -0.7557482518711467 1106 3 -0.14868765792338579 1106 4 -0.05373374355090732 1106 5 -0.1353600553757417 1106 6 -0.16194661129789267 1106 7 -0.09280437676796538 1106 8 -0.13652024241051208 1106 9 -0.06269826871523065 1106 10-11 -0.43310203954460746 1106 12-13 -0.4917521170691188 1106 14-15 -0.14875475304755525 1106 16-17 -0.2341669294189188 1106 18-19 -0.07179525275002874 1106 20-21 -0.06342182806981356 1106 22-23 0.05223222168093855 1106 24-25 0.16898651736202197 1106 26-27 -0.0666051435390358 1106 28-29 0.31193526293383655 1106 30-31 0.24660502601594914 1106 32-33 0.22994593410589204 1106 34-35 0.37759567929104065 1106 36-37 0.343555000605825 1106 38-39 0.49707890904441143 1106 40-41 0.4777144413464711 1106 42-43 0.3198664357871479 1106 44-45 0.47201011786267344 1106 46-47 0.8432148301672342 1106 48-49 0.90794702782717 1106 50-51 0.7775645794146797 1106 52-53 0.7565807203930248 1106 54-55 0.831715456911283 1106 56-57 0.8189338982674954 1106 58-59 0.3716735679816878 1106 60-61 0.9006088641640844 1106 62-63 0.9233650263686499 1106 64-65 1.037653972658795 1106 66-67 1.2607007436512419 1106 68-69 1.1066017878516163 1106 70-71 0.9110796376035246 1106 72-73 0.9235828420107843 1106 74-75 0.4454650617078322 1106 76-77 0.7711825253415974 1106 78-79 0.8311085783982328 1106 80-81 0.7588700218642721 1106 82-83 0.8425356725215316 1106 84-85 0.8809625672463284 1106 86-87 0.7672624379684692 1106 88-89 0.8262238983526586 1106 90-91 0.5096816606526993 1106 92-93 0.9361293440573561 1106 94-95 1.1819425193243518 1106 96-97 1.1685779160762166 1106 98-99 0.9537990760535671 1106 100-101 0.9017958515969227 1107 1 -0.013632245372516394 1107 2 0.09788682024349527 1107 3 -0.023558716325435825 1107 4 0.04167222945628879 1107 5 0.10252406704928774 1107 6 -0.06482656435388634 1107 7 0.008275026549000586 1107 8 -0.036401338182464826 1107 9 0.037471594467696434 1107 10-11 -0.21426112413647758 1107 12-13 0.10779156865311279 1107 14-15 0.1667120618633433 1107 16-17 0.12737450175561094 1107 18-19 0.2675852098955076 1107 20-21 0.16919730778984388 1107 22-23 0.1965155534700571 1107 24-25 0.0817864803009627 1107 26-27 0.11509364271130806 1107 28-29 0.1738550257430589 1107 30-31 0.0865136087393168 1107 32-33 0.08133109115212989 1107 34-35 0.2222727848224011 1107 36-37 0.30828754330325836 1107 38-39 0.34918366830195424 1107 40-41 0.14259754459239815 1107 42-43 -0.13479090384655734 1107 44-45 -0.12456096215833412 1107 46-47 0.011838783964442712 1107 48-49 -0.3044159234684187 1107 50-51 -0.23269361262337185 1107 52-53 -0.16405456791112982 1107 54-55 0.15120802925755328 1107 56-57 0.13047224102050592 1107 58-59 0.07842176897616326 1107 60-61 0.15563913069485835 1107 62-63 0.262809573760471 1107 64-65 -0.03526829177185675 1107 66-67 0.2834585496365989 1107 68-69 0.26375379995822357 1107 70-71 0.2366679819007551 1107 72-73 0.5204851552384966 1107 74-75 0.3909883947323216 1107 76-77 0.4024859936389511 1107 78-79 0.622753626114342 1107 80-81 0.4768963660997336 1107 82-83 0.7110777831487916 1107 84-85 0.43452506145553826 1107 86-87 0.4630984736706196 1107 88-89 0.4889327528571208 1107 90-91 0.6084918462668334 1107 92-93 0.8580177593882787 1107 94-95 1.275892301590634 1107 96-97 1.359258604176528 1107 98-99 1.6282740443972479 1107 100-101 1.8141217418344233 1108 1 -0.08316655012194829 1108 2 0.07145586810035098 1108 3 -0.012997433138774994 1108 4 -0.013703297220665434 1108 5 -0.09844549943870362 1108 6 0.08482737328460388 1108 7 0.13208063975078943 1108 8 0.03600356402856164 1108 9 -0.06482303388980881 1108 10-11 0.12808536733496112 1108 12-13 -0.031955848364056294 1108 14-15 0.05708187323993741 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1.7829824513737442 2316 98-99 2.0516245333075602 2316 100-101 2.2809566560329095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3983.0 3 727.0 4 646.0 5 679.0 6 799.0 7 954.0 8 1097.0 9 1254.0 10 1686.0 11 2221.0 12 3163.0 13 4097.0 14 4770.0 15 6163.0 16 8102.0 17 11149.0 18 9424.0 19 6328.0 20 6215.0 21 6489.0 22 7114.0 23 7798.0 24 8640.0 25 9838.0 26 11449.0 27 13109.0 28 15610.0 29 18828.0 30 23214.0 31 28921.0 32 37038.0 33 47768.0 34 64992.0 35 89729.0 36 125976.0 37 157815.0 38 106472.0 39 10859.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50815097954398 19.193796975260035 15.002626729553548 22.295425315642436 2 15.04296549429735 19.91783638915969 40.979448664551526 24.059749451991436 3 18.71583369267363 25.252349621881276 30.217505204638506 25.81431148080659 4 12.270124968557907 16.50463604255028 37.72471678932464 33.50052219956717 5 13.18986652138382 37.53223946168068 34.49857132093406 14.779322696001437 6 31.10298948656475 36.510577746465486 17.762294160657525 14.624138606312231 7 27.776734872661287 33.3194279317801 21.04385531893197 17.859981876626644 8 26.61408727514604 34.20932175597863 19.990752640074906 19.18583832880043 9 26.478077001973666 16.40101939941659 20.682589663920375 36.43831393468936 10-11 24.471271781516375 26.964023590594127 27.31549493133705 21.24920969655245 12-13 25.64683658362041 24.310602087793967 28.23980367899081 21.802757649594813 14-15 22.706753847981204 25.312748963587218 26.822913196265148 25.157583992166426 16-17 21.34515476962278 28.830712559609783 27.45523363906613 22.368899031701307 18-19 21.45776452316111 26.82895461742067 30.6524017330643 21.06087912635392 20-21 22.155901276737744 25.93565445699541 30.755139567515084 21.153304698751764 22-23 21.87494206740573 25.75578862481925 30.83444950131623 21.53481980645879 24-25 21.51606055760643 26.080243695005827 30.907043193526185 21.496652553861555 26-27 21.1917364688762 26.372982764903224 30.74383018757527 21.691450578645302 28-29 21.205393225616373 26.504119776840835 30.77465478292157 21.515832214621224 30-31 21.346776100168277 26.256275583287366 30.725904773383643 21.671043543160714 32-33 21.078869909843096 26.372227501332652 30.592914918766077 21.95598767005817 34-35 21.645037446998852 26.314411097707524 30.479817556691962 21.56073389860166 36-37 21.633542189894357 26.200577614400384 30.31977358905951 21.84610660664575 38-39 21.377313634804853 26.41951980202608 30.49137803193905 21.711788531230013 40-41 21.54407944164245 26.49713354599794 30.368482850223216 21.59030416213639 42-43 21.271652859046405 26.44910491860263 30.197728984415733 22.08151323793523 44-45 21.489105203306007 26.669180872800286 29.988454348331096 21.85325957556261 46-47 21.740591456253753 26.93256300220566 29.476753045026943 21.850092496513636 48-49 21.596975050306707 27.119095806103783 29.136984309174334 22.146944834415176 50-51 21.909774113673887 27.13515919130366 29.033703020992753 21.921363674029706 52-53 21.870220402646755 27.233279994065256 28.853510973767165 22.04298862952082 54-55 21.732663773118976 27.237589003823494 28.790162169616128 22.239585053441406 56-57 21.684919175734997 27.563142327863954 28.709557456303457 22.042381040097595 58-59 21.824613998121105 27.05748934578053 28.831204033949863 22.2866926221485 60-61 21.58983557998787 27.033182294976 29.106112796287036 22.270869328749086 62-63 21.4792309875635 27.115044862920463 29.417506096607333 21.988218052908703 64-65 21.439559624865627 27.006570411832303 29.223910646105942 22.32995931719613 66-67 21.406668666903762 27.1982818040594 28.985346911776798 22.409702617260038 68-69 21.407574865057256 26.933928303541382 29.10465440235671 22.55384242904465 70-71 21.336409507254167 26.95704960706956 29.166461517608738 22.54007936806753 72-73 21.50342743874112 26.815523047889144 29.02722603949809 22.653823473871647 74-75 21.386341649915714 27.197020164864128 28.671123288940898 22.74551489627926 76-77 21.439517051791213 26.953214392273754 28.833792470156105 22.773476085778924 78-79 21.443907582499072 26.940640063033 28.774042918057102 22.841409436410824 80-81 21.404898496874353 26.948163107120077 28.785722189105222 22.861216206900348 82-83 21.48529728652624 26.8868733302903 28.780639824647174 22.847189558536286 84-85 21.349845186893532 26.8003322139838 28.771781271610415 23.078041327512253 86-87 21.4867354639764 26.697213645243817 28.83509670603485 22.980954184744927 88-89 21.27297467478189 26.514117162514346 28.753932536756295 23.458975625947474 90-91 21.286503569984557 26.643623670451554 28.86155550071886 23.208317258845025 92-93 21.31142602925093 26.615590301592302 28.821169388473873 23.251814280682893 94-95 21.19081698439666 26.488550193151955 28.65999637387545 23.660636448575936 96-97 21.414966017921405 26.61261357290332 28.43603652870692 23.536383880468357 98-99 21.405994190499253 26.90769799401902 28.110730428273982 23.575577387207744 100-101 21.8610009626646 26.118445974359062 28.344796108564474 23.675756954411863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1374.0 1 789.5 2 237.0 3 264.5 4 221.5 5 211.0 6 369.0 7 757.0 8 1299.5 9 1634.0 10 1527.5 11 1357.0 12 1416.0 13 1468.5 14 1535.5 15 1620.0 16 1643.5 17 1737.0 18 1788.5 19 1867.0 20 2091.5 21 2353.5 22 2670.5 23 3176.0 24 3860.0 25 4711.5 26 5938.0 27 7476.0 28 8645.5 29 10027.5 30 11744.5 31 12876.0 32 14444.0 33 15938.0 34 17241.0 35 18777.0 36 21099.5 37 23488.0 38 24232.5 39 24429.5 40 24756.0 41 25860.0 42 26904.5 43 28074.5 44 29654.5 45 30729.0 46 31429.0 47 32028.5 48 32901.5 49 33190.5 50 32133.0 51 30400.5 52 28920.0 53 28530.0 54 28087.5 55 26104.0 56 23478.5 57 20636.5 58 18541.5 59 16600.0 60 13858.5 61 11310.0 62 8937.0 63 6617.0 64 4693.5 65 3421.5 66 2548.5 67 1875.0 68 1410.5 69 1048.5 70 758.0 71 515.0 72 383.5 73 305.0 74 213.5 75 174.5 76 134.5 77 89.0 78 53.5 79 37.5 80 26.5 81 22.0 82 17.5 83 7.5 84 4.5 85 7.5 86 7.5 87 5.5 88 6.0 89 4.5 90 2.5 91 3.0 92 2.5 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3268922007081123 2 0.2829675061292287 3 0.2803089061941911 4 0.2797309496865742 5 0.28111804530485474 6 0.2457471070387011 7 0.24771215916459854 8 0.25060194170268296 9 0.2607739762367402 10-11 0.2676516586773812 12-13 0.2594446762692214 14-15 0.25019737214735116 16-17 0.24372425926204203 18-19 0.23366781602950815 20-21 0.2378868985351114 22-23 0.2363842116153075 24-25 0.23938958545491532 26-27 0.23389899863255492 28-29 0.24800113741840699 30-31 0.2599648371260766 32-33 0.2516422634163934 34-35 0.25013957649658947 36-37 0.2409500680254809 38-39 0.2280616379056243 40-41 0.22453610320916131 42-43 0.23892722024882185 44-45 0.23366781602950815 46-47 0.24407103316661216 48-49 0.24973500694125766 50-51 0.2625656414103526 52-53 0.27747691930686835 54-55 0.2499661895443044 56-57 0.22517185536753986 58-59 0.2131503600091086 60-61 0.2162135294994781 62-63 0.22332239454316583 64-65 0.214190681722819 66-67 0.21800519467309049 68-69 0.21736944251471188 70-71 0.22233986848021714 72-73 0.22378475974925935 74-75 0.22910195961933474 76-77 0.2409500680254809 78-79 0.24008313326405561 80-81 0.23297426822036787 82-83 0.224593898859923 84-85 0.21014498616950078 86-87 0.21459525127815082 88-89 0.22719470314419898 90-91 0.22517185536753986 92-93 0.2271369074934373 94-95 0.22384255540002104 96-97 0.23291647256960618 98-99 0.23673098551987765 100-101 0.21823637727613723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 865117.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.1520358025239 #Duplication Level Percentage of deduplicated Percentage of total 1 80.00237033293553 48.12305444553513 2 11.443886078802608 13.767460902642787 3 3.512778499937329 6.339023342836992 4 1.542018731633379 3.71022263813494 5 0.8516031162472364 2.5612830569022345 6 0.5613787412189372 2.0260844484346383 7 0.37410978053354116 1.5752425438909547 8 0.2695786976299254 1.297256597714643 9 0.19804763664530486 1.0721671677084257 >10 1.152620553753159 12.519337101015502 >50 0.06389608484759962 2.6115614464995955 >100 0.026557089752931043 2.681075412985758 >500 3.8488535419720554E-4 0.18914887923724052 >1k 5.773280312958083E-4 0.9146054436067549 >5k 1.9244267709860277E-4 0.6124765728544919 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5291 0.6115935763601917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3807 0.4400560848994991 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.2973008275181276 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1522 0.17592996091858096 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 972 0.1123547450807232 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1559130152337776E-4 0.0 0.0 0.0 0.0 3 1.1559130152337776E-4 0.0 0.0 0.0 0.0 4 1.1559130152337776E-4 0.0 0.0 0.0 0.0 5 1.1559130152337776E-4 0.0 0.0 0.0 0.0 6 1.1559130152337776E-4 0.0 0.0 0.0 0.0 7 1.1559130152337776E-4 0.0 0.0 0.0 0.0 8 1.1559130152337776E-4 0.0 0.0 0.0 0.0 9 1.1559130152337776E-4 0.0 0.0 0.0 0.0 10-11 1.1559130152337776E-4 0.0 0.0 0.0 0.0 12-13 1.1559130152337776E-4 0.0 0.0 0.0 0.0 14-15 1.1559130152337776E-4 0.0 0.0 0.0 0.0 16-17 1.1559130152337776E-4 0.0 0.0 0.0 0.0 18-19 1.1559130152337776E-4 0.0 0.0 0.0 0.0 20-21 1.1559130152337776E-4 0.0 0.0 0.0 0.0 22-23 1.1559130152337776E-4 0.0 0.0 0.0 0.0 24-25 2.311826030467555E-4 0.0 0.0 2.889782538084444E-4 0.0 26-27 2.311826030467555E-4 0.0 0.0 4.0456955533182216E-4 0.0 28-29 4.0456955533182216E-4 0.0 0.0 0.0010403217137104 0.0 30-31 4.62365206093511E-4 0.0 0.0 0.003930104251794844 0.0 32-33 4.62365206093511E-4 0.0 0.0 0.00826477805892151 0.0 34-35 4.62365206093511E-4 0.0 0.0 0.012021495358431288 0.0 36-37 4.62365206093511E-4 0.0 0.0 0.019361543005165776 0.0 38-39 4.62365206093511E-4 0.0 0.0 0.033637068743302925 0.0 40-41 4.62365206093511E-4 0.0 0.0 0.051958290034758305 0.0 42-43 4.62365206093511E-4 0.0 0.0 0.07946901979732221 0.0 44-45 4.62365206093511E-4 0.0 0.0 0.1055348582908439 0.0 46-47 4.62365206093511E-4 0.0 0.0 0.15743535267484052 0.0 48-49 5.779565076168889E-4 0.0 0.0 0.22800384225486264 0.0 50-51 5.779565076168889E-4 0.0 0.0 0.27666778019620464 0.0 52-53 5.779565076168889E-4 0.0 0.0 0.3493169132036476 0.0 54-55 5.779565076168889E-4 0.0 0.0 0.4445641456589109 0.0 56-57 5.779565076168889E-4 0.0 0.0 0.5918274637996941 0.0 58-59 5.779565076168889E-4 0.0 0.0 0.7589146901517367 0.0 60-61 5.779565076168889E-4 0.0 0.0 0.9530502810602497 0.0 62-63 5.779565076168889E-4 0.0 0.0 1.173482893065331 0.0 64-65 5.779565076168889E-4 0.0 0.0 1.4188254305487003 0.0 66-67 5.779565076168889E-4 0.0 0.0 1.7011571845195506 0.0 68-69 5.779565076168889E-4 0.0 0.0 2.060010380098877 0.0 70-71 5.779565076168889E-4 0.0 0.0 2.4219267451685726 0.0 72-73 5.779565076168889E-4 0.0 0.0 2.806036640130757 0.0 74-75 5.779565076168889E-4 0.0 0.0 3.209681465050392 0.0 76-77 5.779565076168889E-4 0.0 0.0 3.711578896264898 0.0 78-79 5.779565076168889E-4 0.0 0.0 4.265781391418733 0.0 80-81 6.935478091402666E-4 0.0 0.0 4.9526249050706435 0.0 82-83 6.935478091402666E-4 0.0 0.0 5.634209014503241 0.0 84-85 6.935478091402666E-4 0.0 0.0 6.399481226238763 0.0 86-87 6.935478091402666E-4 0.0 0.0 7.152963125218901 0.0 88-89 6.935478091402666E-4 0.0 0.0 8.174212274177943 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2255 0.0 49.33496 1 GTATCAA 4250 0.0 43.291992 1 TCAACGC 5535 0.0 33.142017 4 ATCAACG 5535 0.0 33.138176 3 TATCAAC 5590 0.0 32.897133 2 CAACGCA 5610 0.0 32.69894 5 AACGCAG 5785 0.0 31.700592 6 ACGCAGA 6605 0.0 27.405363 7 ATAGTAC 175 4.9367372E-9 27.15319 3 CGCAGAG 6650 0.0 27.077026 8 GTAGGAC 160 6.214941E-8 26.728922 3 GTACATG 4245 0.0 25.199408 1 GCAGAGT 7120 0.0 24.555637 9 TACATGG 4295 0.0 24.450516 2 ACATGGG 4225 0.0 23.618454 3 GTATAGG 225 2.7812348E-9 23.243187 1 GAGTACT 3995 0.0 22.708412 12-13 GTGGTAT 1240 0.0 22.62121 1 TGGTATC 1160 0.0 22.530125 2 CATGGGA 2575 0.0 22.331474 4 >>END_MODULE