FastQCFastQC Report
Thu 26 May 2016
SRR937151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865117
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52670.6088193851236307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44130.5101044136226661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.34584917415794625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.1278439794848558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.050.716811
TATACCG459.628049E-442.218395
GTATCAA44950.041.432021
CTAGCGG550.002580420834.5423243
ATCAACG57350.032.3815423
TCAACGC57950.032.0462764
TATCAAC58400.032.0433272
CAACGCA59250.031.3431515
AACGCAG60400.030.8250226
GTAAGGT4000.028.4974174
TAAGGTG4650.027.5781465
GTACATG40900.026.4844681
ACGCAGA70100.026.428727
CGCAGAG69850.026.0472538
TACATGG41850.025.535322
ACATGGG41750.025.027673
ACACCGT1951.5701517E-824.3567666
GCAGAGT75050.024.115929
TGGTATC12950.023.4727752
AGTACTT47650.023.12888312-13
Produced by FastQC (version 0.11.5)