FastQCFastQC Report
Thu 26 May 2016
SRR937150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812291
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51300.6315470687229084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37760.4648580373289868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.30457065263557026No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12160.1497000459195042No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.11510653201869774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.055.267761
GTATCAA39200.042.0943761
CCGTATG550.002577486734.5500769
ATCAACG48800.033.699423
TCAACGC49400.033.195954
TATCAAC49600.033.155882
CAACGCA49850.032.8009385
AACGCAG51200.032.011126
TACGTTG600.003936961331.6885015
ATACCGT650.005839249629.234686
GTACATG38250.028.4698221
ACGCAGA58950.027.7221157
CGCAGAG59200.027.52488
GTACGGC700.00834435427.1733191
TACATGG39250.027.1252982
GCAGAGT64150.024.9565739
ACATGGG41200.024.9185473
GAGTACT37650.024.03633912-13
CCATATA2903.6379788E-1222.9454292
GTACTTT39300.022.90559614-15
Produced by FastQC (version 0.11.5)