##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937150_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 812291 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.918084775037517 33.0 31.0 34.0 30.0 34.0 2 32.031499795024196 33.0 31.0 34.0 30.0 34.0 3 32.065057965679785 34.0 31.0 34.0 30.0 34.0 4 35.67437039189158 37.0 35.0 37.0 35.0 37.0 5 35.672851231886106 37.0 35.0 37.0 35.0 37.0 6 35.71808748342651 37.0 35.0 37.0 35.0 37.0 7 35.66938449398061 37.0 35.0 37.0 35.0 37.0 8 35.66589559652883 37.0 35.0 37.0 35.0 37.0 9 37.32355153510257 39.0 37.0 39.0 35.0 39.0 10-11 37.22379233550538 39.0 37.0 39.0 34.0 39.0 12-13 37.08720335446287 39.0 37.0 39.0 33.5 39.0 14-15 38.30984585573397 40.0 38.0 41.0 34.0 41.0 16-17 38.29157161657584 40.0 38.0 41.0 34.0 41.0 18-19 38.284876971430194 40.0 38.0 41.0 34.0 41.0 20-21 38.23810432468167 40.0 38.0 41.0 34.0 41.0 22-23 38.09034385460383 40.0 38.0 41.0 33.5 41.0 24-25 37.9387153126158 40.0 38.0 41.0 33.0 41.0 26-27 37.788363406710154 40.0 38.0 41.0 33.0 41.0 28-29 37.81024965191046 40.0 38.0 41.0 33.0 41.0 30-31 37.62001918031838 40.0 37.0 41.0 33.0 41.0 32-33 37.392707170213626 40.0 37.0 41.0 33.0 41.0 34-35 37.275068294490524 40.0 36.5 41.0 32.5 41.0 36-37 37.30021691733627 40.0 37.0 41.0 32.5 41.0 38-39 37.21547758131014 40.0 37.0 41.0 32.0 41.0 40-41 37.03654355397265 40.0 36.5 41.0 31.5 41.0 42-43 36.78207563545577 40.0 36.0 41.0 31.0 41.0 44-45 36.515926558339316 40.0 36.0 41.0 30.5 41.0 46-47 36.1110476417934 39.5 36.0 41.0 29.5 41.0 48-49 35.751432060677764 39.0 35.0 41.0 28.0 41.0 50-51 34.8334285373099 38.5 34.0 40.0 26.0 40.5 52-53 34.883625449500244 38.5 35.0 40.0 26.0 40.5 54-55 35.18805822051457 39.0 35.0 40.0 26.0 41.0 56-57 34.99369376245705 39.0 35.0 40.0 26.0 41.0 58-59 34.75994132644582 38.5 35.0 40.0 25.0 41.0 60-61 34.475802391015044 38.0 34.0 40.0 24.5 41.0 62-63 34.146032025468706 38.0 34.0 40.0 23.0 41.0 64-65 33.7641657977252 37.0 34.0 40.0 22.0 41.0 66-67 33.39301309506076 37.0 33.5 39.5 21.5 41.0 68-69 32.92305466883174 36.0 33.0 39.0 19.5 41.0 70-71 32.50162441785025 35.5 32.5 39.0 18.0 40.0 72-73 32.103482003370715 35.0 32.0 38.0 15.5 40.0 74-75 31.65680402712821 35.0 32.0 37.0 10.0 39.5 76-77 31.2416720116313 35.0 31.0 37.0 7.5 39.0 78-79 30.802987476163096 35.0 31.0 36.0 4.5 39.0 80-81 30.339270655467068 34.5 31.0 36.0 2.0 37.5 82-83 29.880833962213046 34.0 30.0 35.5 2.0 37.0 84-85 29.469702360361005 34.0 30.0 35.0 2.0 37.0 86-87 29.06028750780201 34.0 29.0 35.0 2.0 36.0 88-89 28.700934763526863 34.0 29.0 35.0 2.0 36.0 90-91 28.31922919249382 34.0 29.0 35.0 2.0 35.5 92-93 27.89592892202425 34.0 28.0 35.0 2.0 35.0 94-95 27.406223262353024 34.0 27.0 35.0 2.0 35.0 96-97 26.83460422434817 33.0 25.5 35.0 2.0 35.0 98-99 26.122489354184644 33.0 24.5 35.0 2.0 35.0 100-101 24.324991905610183 31.0 12.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1883133389647682 1101 2 0.1625683212550335 1101 3 0.21433592926329226 1101 4 0.14416251367965316 1101 5 0.17285767917609718 1101 6 0.12396008569445627 1101 7 0.1093656916418837 1101 8 0.2259198861101197 1101 9 0.21633706973956635 1101 10-11 0.2685754575918011 1101 12-13 0.10120369567608378 1101 14-15 0.18361576976855787 1101 16-17 0.30584535919739864 1101 18-19 0.1893313454082488 1101 20-21 0.1030618293016019 1101 22-23 0.11334469293046112 1101 24-25 0.1314647299803937 1101 26-27 0.19065769263234955 1101 28-29 0.0583859784215619 1101 30-31 0.1647076338907425 1101 32-33 0.1512336162180219 1101 34-35 0.21570379132221973 1101 36-37 0.39219139953660687 1101 38-39 0.3242328998410713 1101 40-41 0.2910662855270729 1101 42-43 0.36014577895359423 1101 44-45 0.5524809119833236 1101 46-47 0.4588073089789688 1101 48-49 0.6311048209547607 1101 50-51 0.5505422076503947 1101 52-53 0.6577043156439046 1101 54-55 0.7871888796998618 1101 56-57 0.8977604622658291 1101 58-59 0.9030488104021401 1101 60-61 0.6070755904048681 1101 62-63 0.7145997857352526 1101 64-65 0.7771596962704947 1101 66-67 0.7823064826030617 1101 68-69 0.820292134232723 1101 70-71 0.8357144022180805 1101 72-73 0.8190172072423074 1101 74-75 0.6927973663225337 1101 76-77 0.5974482462218305 1101 78-79 0.5978707648544663 1101 80-81 0.5834080296549473 1101 82-83 0.6091577448847012 1101 84-85 0.6755140839098708 1101 86-87 0.6916214710561626 1101 88-89 0.7778341258997514 1101 90-91 0.6946015288241831 1101 92-93 0.8833918471065623 1101 94-95 0.9996314382183726 1101 96-97 0.9067658004267223 1101 98-99 1.1429674835354398 1101 100-101 1.5979971078468296 1102 1 0.06428428044821288 1102 2 0.01916781576779769 1102 3 0.10250333879733375 1102 4 0.04738900620772313 1102 5 0.0962683934810471 1102 6 -0.03208810035585685 1102 7 0.12612705191299511 1102 8 0.1623086137662142 1102 9 0.1787055154470707 1102 10-11 0.24253435740861562 1102 12-13 0.21420604650404584 1102 14-15 0.31198963325685014 1102 16-17 0.28936829511553697 1102 18-19 0.27359958963160125 1102 20-21 0.20906271341967653 1102 22-23 0.2621297305512513 1102 24-25 0.12980943157170088 1102 26-27 0.10105997174012771 1102 28-29 0.2990896814871107 1102 30-31 0.32645897379806854 1102 32-33 0.38446660232951047 1102 34-35 0.32615999517120287 1102 36-37 0.32290456837300496 1102 38-39 0.5907542505925747 1102 40-41 0.5214322145671773 1102 42-43 0.5772519560421188 1102 44-45 0.6727492380495121 1102 46-47 0.802854830594633 1102 48-49 0.6166858082163529 1102 50-51 0.6189208255616947 1102 52-53 0.6303553453022417 1102 54-55 0.524112870313779 1102 56-57 0.5140676216838429 1102 58-59 0.48903777346410493 1102 60-61 0.5335687376821383 1102 62-63 0.3910657418050292 1102 64-65 0.5259950510861344 1102 66-67 0.4970004496010034 1102 68-69 0.4598558526545773 1102 70-71 0.48873373005142895 1102 72-73 0.4116082493278306 1102 74-75 0.6038426771045771 1102 76-77 0.37819315608162896 1102 78-79 0.5869738211461915 1102 80-81 0.399724557924646 1102 82-83 0.6590066317681966 1102 84-85 0.7211203347646418 1102 86-87 0.5673292501106424 1102 88-89 0.6993125145434114 1102 90-91 0.6994165263096725 1102 92-93 0.6320656699964999 1102 94-95 0.5578025845242998 1102 96-97 0.6961039395143267 1102 98-99 0.8194864759208222 1102 100-101 1.2902427139092296 1103 1 0.06660712880487196 1103 2 -0.10416433877322007 1103 3 0.052798081697353894 1103 4 -0.1082246896557919 1103 5 -0.08551281101163255 1103 6 0.004839809994969357 1103 7 0.011890983288459722 1103 8 -0.0038674870487653834 1103 9 0.1286099794842741 1103 10-11 0.18844123640133148 1103 12-13 0.0720322638312112 1103 14-15 0.09156460243120534 1103 16-17 0.021806082446161668 1103 18-19 -0.019034594359496282 1103 20-21 -0.14763082119579707 1103 22-23 -0.2636755683667289 1103 24-25 -0.14954770753968916 1103 26-27 -0.030546027461163305 1103 28-29 0.058698911834333956 1103 30-31 -0.14010512000869824 1103 32-33 -0.08278594204664103 1103 34-35 -0.0853275488486176 1103 36-37 0.07573148490779147 1103 38-39 0.10921215493290504 1103 40-41 0.1803821055849184 1103 42-43 0.04429877202547061 1103 44-45 0.17898126202695153 1103 46-47 0.40820339903428504 1103 48-49 0.4351336950630511 1103 50-51 0.28325142167652473 1103 52-53 0.38532677863749853 1103 54-55 0.28387959045191025 1103 56-57 0.17678382138290516 1103 58-59 0.3822064142717352 1103 60-61 0.4017316301964442 1103 62-63 0.20712354879413652 1103 64-65 0.2801230652423996 1103 66-67 0.4663425490256401 1103 68-69 0.4393349497086305 1103 70-71 0.43415603800128366 1103 72-73 0.4484159833707757 1103 74-75 0.4094540534813085 1103 76-77 0.3925769995451809 1103 78-79 0.413772700800461 1103 80-81 0.3602789959432542 1103 82-83 0.3213486881646723 1103 84-85 0.353256286271904 1103 86-87 0.41673354539677376 1103 88-89 0.20535948381457914 1103 90-91 0.46334756561455137 1103 92-93 0.48210076483998066 1103 94-95 0.3544827602405505 1103 96-97 0.4407580267189637 1103 98-99 0.5442933198186886 1103 100-101 0.8521441014329483 1104 1 0.1268548392816875 1104 2 0.16295224960596855 1104 3 0.1499034665736545 1104 4 0.12439415484164584 1104 5 0.04092396114612029 1104 6 -0.024690979081100295 1104 7 0.08996973671889208 1104 8 -0.026992750433869617 1104 9 0.08187060996396411 1104 10-11 0.11401318036325847 1104 12-13 0.1277029836305772 1104 14-15 0.14743760198923184 1104 16-17 0.14636066611782894 1104 18-19 0.17721603519512996 1104 20-21 0.13945863209381315 1104 22-23 0.1458282911886286 1104 24-25 0.22814025948706984 1104 26-27 0.20535513503074299 1104 28-29 0.22005833870835545 1104 30-31 0.24472985958858828 1104 32-33 0.23254394458213312 1104 34-35 0.4749117333049142 1104 36-37 0.5266023773849895 1104 38-39 0.543636921869215 1104 40-41 0.708518726649551 1104 42-43 0.7929597992530617 1104 44-45 0.8240209882092344 1104 46-47 0.9012837456233811 1104 48-49 1.056050121042837 1104 50-51 1.085986104226805 1104 52-53 1.1188817837253282 1104 54-55 1.1311807796677655 1104 56-57 1.0922391050165459 1104 58-59 1.0275538770380805 1104 60-61 0.9265555090752784 1104 62-63 0.772052062222933 1104 64-65 0.933215540394265 1104 66-67 1.1339537360830292 1104 68-69 1.2770017431427618 1104 70-71 1.1118084416151888 1104 72-73 1.2894911085108127 1104 74-75 1.2018157313343458 1104 76-77 1.063997583155178 1104 78-79 0.9661876209883502 1104 80-81 1.0263946695410162 1104 82-83 1.1721809336961186 1104 84-85 1.1244840462908208 1104 86-87 1.0471659594946878 1104 88-89 0.9096005749740925 1104 90-91 1.1024985662209765 1104 92-93 1.2414079977803318 1104 94-95 1.387992745747944 1104 96-97 1.3812826384507773 1104 98-99 1.4386354480731036 1104 100-101 1.6887612602657285 1105 1 0.14886723281893666 1105 2 0.12069141940875383 1105 3 0.2392195583650576 1105 4 0.20276714938056273 1105 5 0.14780661868269362 1105 6 0.17880015020216433 1105 7 0.12936137815518123 1105 8 0.2574468522938389 1105 9 0.28170423097073183 1105 10-11 0.23485526179842253 1105 12-13 0.12159789322566894 1105 14-15 0.33527354324726133 1105 16-17 0.17420962341955004 1105 18-19 0.21199377210128034 1105 20-21 0.2849703606810081 1105 22-23 0.10505298771673921 1105 24-25 0.2850390455995111 1105 26-27 0.3729562215874367 1105 28-29 0.37043410623049766 1105 30-31 0.2537426209705558 1105 32-33 0.035760888075522246 1105 34-35 0.2058194608734638 1105 36-37 0.14868635916253226 1105 38-39 0.43617335264365664 1105 40-41 0.38514955203650203 1105 42-43 0.41732724782553987 1105 44-45 0.35223722430895066 1105 46-47 0.5838187616104804 1105 48-49 0.7203966307624299 1105 50-51 0.6881872303975456 1105 52-53 0.6394938207361633 1105 54-55 0.48329928225682295 1105 56-57 0.7894799867120312 1105 58-59 0.8139209860615964 1105 60-61 0.5266619010845588 1105 62-63 0.7010025729611655 1105 64-65 0.8876871173162542 1105 66-67 1.0514204178407596 1105 68-69 0.9139760789000988 1105 70-71 0.8413016647837495 1105 72-73 1.0245639700597273 1105 74-75 0.8084154313994283 1105 76-77 0.95245772859532 1105 78-79 1.0113405595016935 1105 80-81 1.017486885380979 1105 82-83 1.1352812987112593 1105 84-85 1.1309844282834014 1105 86-87 1.256012624840885 1105 88-89 1.2467636433649112 1105 90-91 1.3521479819137987 1105 92-93 1.3845346778403176 1105 94-95 1.33741494659116 1105 96-97 1.319171209798501 1105 98-99 1.4113741934396735 1105 100-101 1.7439784301250967 1106 1 0.052514029376077076 1106 2 0.06496314307327467 1106 3 0.03244279469706868 1106 4 -0.04926801234939404 1106 5 0.008384294716265117 1106 6 0.1282884857181159 1106 7 0.33033172460751814 1106 8 0.21615214621351697 1106 9 0.015906944075403828 1106 10-11 -0.3079456139126009 1106 12-13 -0.2819181131588877 1106 14-15 -0.25492918985082014 1106 16-17 -0.362112556927606 1106 18-19 0.02303862729905859 1106 20-21 0.059169041782908494 1106 22-23 0.06187890183174716 1106 24-25 0.0065542789058596895 1106 26-27 -0.05316815736751579 1106 28-29 0.21499456794479244 1106 30-31 0.18583003212486915 1106 32-33 0.270468434585176 1106 34-35 0.41226550034710385 1106 36-37 0.31324705413319265 1106 38-39 0.3731091638259727 1106 40-41 0.1956187638632656 1106 42-43 0.24405692703140858 1106 44-45 0.3330727152635973 1106 46-47 0.6806751323414701 1106 48-49 0.8263258407897851 1106 50-51 0.5407667125017852 1106 52-53 0.4948857080800124 1106 54-55 0.4622653901078948 1106 56-57 0.5535811666559809 1106 58-59 0.567414833092073 1106 60-61 0.5237895156384198 1106 62-63 0.8427482968722799 1106 64-65 0.9398723961742306 1106 66-67 0.9664517908210115 1106 68-69 0.8621006977758654 1106 70-71 0.9504947977165443 1106 72-73 0.9867589457177566 1106 74-75 0.7683744782472992 1106 76-77 1.0465216874888839 1106 78-79 1.2035687908520138 1106 80-81 0.9046232970318009 1106 82-83 0.9482413552259423 1106 84-85 0.7917180528539625 1106 86-87 0.8361270204236781 1106 88-89 1.126854879923517 1106 90-91 1.1009217199135897 1106 92-93 1.3183815083862385 1106 94-95 1.3117459173708 1106 96-97 1.4762310467189828 1106 98-99 1.6004284721943556 1106 100-101 1.6517613617576572 1107 1 -0.10671689833564812 1107 2 -0.08184091806083771 1107 3 -0.04222806336483487 1107 4 0.02316425962164459 1107 5 0.011618669473968168 1107 6 -0.0019682040100903464 1107 7 0.09311802769502009 1107 8 0.15096620289482132 1107 9 -0.026132174136563435 1107 10-11 0.19412793045260912 1107 12-13 0.09958469698109695 1107 14-15 0.181870905851234 1107 16-17 0.18667444177199144 1107 18-19 0.05048152175432108 1107 20-21 -0.17980583864491706 1107 22-23 0.02524739237563267 1107 24-25 0.08346372305862104 1107 26-27 0.16025571630579094 1107 28-29 0.08190336965325429 1107 30-31 0.15376652318271056 1107 32-33 0.15553459527157543 1107 34-35 0.2182240604743484 1107 36-37 0.17607245771005609 1107 38-39 0.2365792270292033 1107 40-41 0.1891981976704571 1107 42-43 0.2355467553658528 1107 44-45 0.5056000246847105 1107 46-47 0.5191053750746519 1107 48-49 0.5170930300516616 1107 50-51 0.5585469123684277 1107 52-53 0.4535156180713429 1107 54-55 0.4940126613811131 1107 56-57 0.5067608228818159 1107 58-59 0.5502499397932041 1107 60-61 0.5711996897408298 1107 62-63 0.28716634202503855 1107 64-65 0.4082624750619601 1107 66-67 0.5541659296908819 1107 68-69 0.5655406547099417 1107 70-71 0.5407100387106922 1107 72-73 0.3959084602292471 1107 74-75 0.23383589725169074 1107 76-77 0.4106497022695308 1107 78-79 0.45154287597809883 1107 80-81 0.5812877262200367 1107 82-83 0.45302368845072394 1107 84-85 0.48147006935370484 1107 86-87 0.28043596081149147 1107 88-89 0.18131975705050607 1107 90-91 0.11355295023873424 1107 92-93 0.4068693495740092 1107 94-95 0.4279807063943082 1107 96-97 0.4720119599203265 1107 98-99 0.889705633742512 1107 100-101 1.0422822068599054 1108 1 -0.32960824143610523 1108 2 -0.3991993197181003 1108 3 -0.40438315287374493 1108 4 -0.21089402600966167 1108 5 -0.35929689830489764 1108 6 -0.11871283873578165 1108 7 -0.11204786867587302 1108 8 -0.32044888403573424 1108 9 -0.2772224180281597 1108 10-11 -0.24874892051700925 1108 12-13 -0.17016898124914093 1108 14-15 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-1.2414220330737038 2316 72-73 -1.146370144938139 2316 74-75 -0.8962945226213925 2316 76-77 -1.1525303745000954 2316 78-79 -1.2751266422021459 2316 80-81 -1.5067963885140507 2316 82-83 -1.31431492755733 2316 84-85 -1.5524955133690703 2316 86-87 -1.4459699152730359 2316 88-89 -1.5302428846801668 2316 90-91 -1.2331824969019465 2316 92-93 -1.7152190645542582 2316 94-95 -1.394612130652753 2316 96-97 -1.1644643067601024 2316 98-99 -0.9454830270662633 2316 100-101 -1.7358786406295934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3506.0 3 690.0 4 580.0 5 603.0 6 655.0 7 737.0 8 845.0 9 1065.0 10 1357.0 11 1901.0 12 2551.0 13 3395.0 14 3832.0 15 5155.0 16 7013.0 17 9767.0 18 8194.0 19 5040.0 20 4783.0 21 4739.0 22 5220.0 23 5578.0 24 6296.0 25 6840.0 26 7747.0 27 9149.0 28 10782.0 29 13019.0 30 16184.0 31 20402.0 32 26655.0 33 35759.0 34 49985.0 35 73829.0 36 114162.0 37 170683.0 38 151061.0 39 22528.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50442797089114 19.131008396406234 15.004823887942504 22.35973974476012 2 15.10308304553385 19.91564192764122 40.9507075730879 24.03056745373703 3 18.5612542999879 25.249726504717945 30.39452466149557 25.794494533798584 4 12.270790887201334 16.567759461628697 37.69018954127307 33.471260109896896 5 13.160198604434642 37.46022416469202 34.59327703491136 14.786300195961974 6 31.072980649874015 36.4215963298677 17.72445381116784 14.780969209090438 7 27.75937434071486 33.384163832126106 21.099962123875898 17.756499703283133 8 26.556436916478816 34.2797989855507 19.978926302516133 19.18483779545435 9 26.335009549000933 16.410340556358847 20.69024225346552 36.564407641174704 10-11 24.45795026840254 26.937619547109275 27.41043993336213 21.193990251126056 12-13 25.700492629072208 24.384306201203657 28.203184043383438 21.712017126340694 14-15 22.641069456696748 25.42057112540947 26.839871438177433 25.09848797971635 16-17 21.290784665847823 28.890926061531797 27.537981582780326 22.280307689840054 18-19 21.330030177487053 26.873755456252514 30.749699582252777 21.04651478400766 20-21 22.104455433635437 25.86724928432157 30.97744926717366 21.050846014869336 22-23 21.80376781681657 25.81801448477039 30.92259758652957 21.45562011188347 24-25 21.491576774311337 26.111660399027688 30.988391349856382 21.408371476804597 26-27 21.130252803150064 26.429762271163714 30.745987327461094 21.693997598225128 28-29 21.14554211873043 26.521266017060192 30.90142151794725 21.43177034626213 30-31 21.37700293916593 26.231562348075226 30.797202486568924 21.59423222618992 32-33 21.04003059820729 26.401070937255167 30.702648348868916 21.856250115668626 34-35 21.605587226376446 26.33578885022581 30.512450334394515 21.546173589003235 36-37 21.577480634930033 26.2337329094545 30.44433259438274 21.744453861232728 38-39 21.313506891800035 26.450312792606965 30.593534920386233 21.642645395206767 40-41 21.505806103825854 26.47783182868679 30.500070621860953 21.516291445626408 42-43 21.19117674272285 26.550330933831738 30.26653589691396 21.991956426531456 44-45 21.424812983194858 26.667637194491927 30.117555182834487 21.789994639478728 46-47 21.67165626490171 26.944200602206365 29.577635084658667 21.806508048233265 48-49 21.497429266264348 27.165196941523984 29.228014890143854 22.109358902067815 50-51 21.71037005001576 27.298414635425374 29.087679662210057 21.903535652348804 52-53 21.80349891179659 27.41297612370037 28.797434962337114 21.986090002165916 54-55 21.651811052379614 27.337291540196528 28.832600423459038 22.17829698396482 56-57 21.7055860481095 27.601439053920114 28.721066424562846 21.971908473407538 58-59 21.770733620834278 27.103337510978655 28.905317122753694 22.220611745433374 60-61 21.558871998302717 27.103346704362114 29.185230687251146 22.152550610084027 62-63 21.459389743611883 27.153195707270854 29.426745856445603 21.960668692671657 64-65 21.264441544280004 27.104771044626624 29.323581873040226 22.307205538053147 66-67 21.275503973692096 27.29284439384389 29.07067534880049 22.36097628366352 68-69 21.360814823723135 26.964362254888357 29.19963859619909 22.475184325189414 70-71 21.31425705969539 27.076120672480585 29.24693498536034 22.362687282463682 72-73 21.376531803031163 26.857839303469085 29.173214942157244 22.59241395134251 74-75 21.264120382078385 27.220432780381408 28.843698457598453 22.671748379941747 76-77 21.330662870283103 27.028507419232266 28.94635552112273 22.6944741893619 78-79 21.290674759212827 27.028364004206644 28.880459153305022 22.800502083275507 80-81 21.253945333727277 27.01177323976588 28.921385698045732 22.812895728461115 82-83 21.30591017365013 26.994410678798452 28.937227148977428 22.76245199857399 84-85 21.213945272797243 26.928675041198353 28.950539772446938 22.906839913557466 86-87 21.33669988608898 26.867952642125122 28.898307123108395 22.897040348677503 88-89 21.20496328234327 26.55642221327263 28.8876403316338 23.3509741727503 90-91 21.118987394862096 26.799965213594085 28.97966660560542 23.101380785938396 92-93 21.19639427613539 26.833113156229533 28.846160962414473 23.1243316052206 94-95 20.99854696700949 26.645701711322555 28.888590113948375 23.467161207719577 96-97 21.19737767197464 26.82489854822197 28.55124394064608 23.426479839157302 98-99 21.18299705416488 27.200124346809172 28.189470983277324 23.42740761574862 100-101 21.33929661703705 26.8686346654102 28.212778711767903 23.579290005784845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1184.0 1 677.5 2 208.5 3 247.0 4 211.0 5 203.0 6 356.5 7 742.5 8 1315.5 9 1670.0 10 1537.0 11 1384.5 12 1438.5 13 1447.0 14 1487.5 15 1554.5 16 1615.5 17 1695.5 18 1794.5 19 1925.5 20 2062.5 21 2292.5 22 2675.5 23 3139.0 24 3646.0 25 4379.5 26 5575.0 27 7019.5 28 8045.5 29 9419.5 30 11211.0 31 12308.5 32 13652.5 33 15071.5 34 16387.0 35 17808.5 36 20034.0 37 22165.0 38 22792.0 39 23052.0 40 23345.0 41 24379.0 42 25513.5 43 26570.0 44 27768.0 45 28913.5 46 29768.5 47 29986.0 48 30693.0 49 31009.0 50 30041.5 51 28558.5 52 27351.0 53 27089.5 54 26472.0 55 24323.5 56 21480.5 57 18901.5 58 17146.0 59 15233.0 60 12665.5 61 10321.0 62 8239.0 63 6039.5 64 4099.0 65 2944.5 66 2161.0 67 1645.0 68 1289.0 69 933.0 70 664.5 71 468.0 72 329.5 73 282.0 74 219.5 75 166.0 76 132.5 77 81.0 78 50.0 79 39.5 80 28.0 81 15.5 82 14.0 83 12.5 84 8.5 85 5.0 86 5.0 87 6.5 88 6.0 89 5.0 90 3.5 91 4.5 92 4.5 93 3.0 94 2.5 95 2.0 96 3.5 97 2.5 98 2.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34199566411544635 2 0.2966917028503332 3 0.29607615989836156 4 0.3005080691525574 5 0.3003849605621631 6 0.2286126523622692 7 0.21593246755165327 8 0.2218416798905811 9 0.21359340433416102 10-11 0.23895377395539283 12-13 0.22695068639194574 14-15 0.2198719424442718 16-17 0.22153390841459525 18-19 0.21580935896125894 20-21 0.20885372360397939 22-23 0.21119278682147163 24-25 0.2025751854938686 26-27 0.20195964254189694 28-29 0.21531692459968166 30-31 0.2286126523622692 32-33 0.2198719424442718 34-35 0.2303361726277898 36-37 0.20706864904326158 38-39 0.2080535177664162 40-41 0.20134409958992527 42-43 0.2100232552127255 44-45 0.2136549586293582 46-47 0.21808686788355405 48-49 0.22430385169846767 50-51 0.22732001216312875 52-53 0.24750982098779872 54-55 0.22442696028886197 56-57 0.199374362143616 58-59 0.2002976765715735 60-61 0.194880898594223 62-63 0.20491424871136085 64-65 0.19808172194447554 66-67 0.19500400718461736 68-69 0.1924802810815336 70-71 0.2031907284458402 72-73 0.2025136311986714 74-75 0.20583756313931828 76-77 0.20866906071838787 78-79 0.20540668307293813 80-81 0.20700709474806442 82-83 0.20165187106591112 84-85 0.1943884642326457 86-87 0.19334204121429388 88-89 0.1942038013470542 90-91 0.2006054480475593 92-93 0.19451157282304002 94-95 0.1936498126902797 96-97 0.19217250960554777 98-99 0.20374471710261471 100-101 0.1910645322919988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 812291.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.54466823756247 #Duplication Level Percentage of deduplicated Percentage of total 1 80.07779690844302 48.482836470165886 2 11.376690946483476 13.775959579922455 3 3.5594845072410006 6.465234257629499 4 1.5784818578866031 3.822746416190225 5 0.8616341194341197 2.608367595165153 6 0.527629054872903 1.9167075647867138 7 0.3466009586244445 1.468938803551668 8 0.2598390313215713 1.2585494357227314 9 0.1957689819360103 1.0667491256268997 >10 1.1287381037576496 12.369838626684247 >50 0.062092466359869695 2.5734818868232234 >100 0.024021625080216723 2.4432373635140148 >500 4.071461863750274E-4 0.19486244580218334 >1k 6.107192795625411E-4 0.9202043139526255 >5k 2.035730931875137E-4 0.6322861144624925 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5130 0.6315470687229084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3776 0.4648580373289868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 0.30457065263557026 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1216 0.1497000459195042 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.11510653201869774 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.155429519716456E-5 0.0 16-17 6.155429519716456E-5 0.0 0.0 1.2310859039432913E-4 0.0 18-19 1.2310859039432913E-4 0.0 0.0 1.2310859039432913E-4 0.0 20-21 1.2310859039432913E-4 0.0 0.0 1.2310859039432913E-4 0.0 22-23 1.2310859039432913E-4 0.0 0.0 1.8466288559149368E-4 0.0 24-25 1.2310859039432913E-4 0.0 0.0 3.077714759858228E-4 0.0 26-27 1.2310859039432913E-4 0.0 0.0 4.924343615773165E-4 0.0 28-29 2.4621718078865826E-4 0.0 0.0 0.0016004116751262786 0.0 30-31 2.4621718078865826E-4 0.0 0.0 0.004308800663801519 0.0 32-33 2.4621718078865826E-4 0.0 0.0 0.008863818508391698 0.0 34-35 3.077714759858228E-4 0.0 0.0 0.01348039064817904 0.0 36-37 3.6932577118298736E-4 0.0 0.0 0.020066700234275647 0.0 38-39 3.6932577118298736E-4 0.0 0.0 0.03416263383442633 0.0 40-41 3.6932577118298736E-4 0.0 0.0 0.051828716556012566 0.0 42-43 4.3088006638015194E-4 0.0 0.0 0.07755841194842736 0.0 44-45 4.924343615773165E-4 0.0 0.0 0.10304189016005348 0.0 46-47 4.924343615773165E-4 0.0 0.0 0.1553014867824462 0.0 48-49 4.924343615773165E-4 0.0 0.0 0.21740977063638522 0.0 50-51 4.924343615773165E-4 0.0 0.0 0.2597591257320344 0.0 52-53 4.924343615773165E-4 0.0 0.0 0.3262377645449722 0.0 54-55 4.924343615773165E-4 0.0 0.0 0.41659946989440977 0.0 56-57 4.924343615773165E-4 0.0 0.0 0.5550966340880301 0.0 58-59 4.924343615773165E-4 0.0 0.0 0.7205545795780084 0.0 60-61 4.924343615773165E-4 0.0 0.0 0.9118653290507959 0.0 62-63 4.924343615773165E-4 0.0 0.0 1.1323528144470392 0.0 64-65 4.924343615773165E-4 0.0 0.0 1.3845407618698224 0.0 66-67 4.924343615773165E-4 0.0 0.0 1.6720608747357781 0.0 68-69 4.924343615773165E-4 0.0 0.0 2.026921386547432 0.0 70-71 5.539886567744811E-4 0.0 0.0 2.3849827217093384 0.0 72-73 6.770972471688101E-4 0.0 0.0 2.77135903266194 0.0 74-75 7.386515423659747E-4 0.0 0.0 3.188266274032336 0.0 76-77 7.386515423659747E-4 0.0 0.0 3.7031063990614204 0.0 78-79 7.386515423659747E-4 0.0 0.0 4.2829478598187105 0.0 80-81 7.386515423659747E-4 0.0 0.0 4.986513453922301 0.0 82-83 7.386515423659747E-4 0.0 0.0 5.701281929751776 0.0 84-85 7.386515423659747E-4 1.2310859039432913E-4 0.0 6.508812728443378 0.0 86-87 7.386515423659747E-4 1.2310859039432913E-4 0.0 7.3230529453114706 0.0 88-89 7.386515423659747E-4 1.2310859039432913E-4 0.0 8.409178484065439 1.2310859039432913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2065 0.0 55.26776 1 GTATCAA 3920 0.0 42.094376 1 CCGTATG 55 0.0025774867 34.550076 9 ATCAACG 4880 0.0 33.69942 3 TCAACGC 4940 0.0 33.19595 4 TATCAAC 4960 0.0 33.15588 2 CAACGCA 4985 0.0 32.800938 5 AACGCAG 5120 0.0 32.01112 6 TACGTTG 60 0.0039369613 31.688501 5 ATACCGT 65 0.0058392496 29.23468 6 GTACATG 3825 0.0 28.469822 1 ACGCAGA 5895 0.0 27.722115 7 CGCAGAG 5920 0.0 27.5248 8 GTACGGC 70 0.008344354 27.173319 1 TACATGG 3925 0.0 27.125298 2 GCAGAGT 6415 0.0 24.956573 9 ACATGGG 4120 0.0 24.918547 3 GAGTACT 3765 0.0 24.036339 12-13 CCATATA 290 3.6379788E-12 22.945429 2 GTACTTT 3930 0.0 22.905596 14-15 >>END_MODULE