FastQCFastQC Report
Thu 26 May 2016
SRR937150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812291
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.6501364658724521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42440.5224728576335328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.3727728117140286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.12963334568522855No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.053.7732931
GTATCAA43000.041.227751
ATCAACG55050.032.0148773
TCAACGC55800.031.6697044
TATCAAC56150.031.6415042
CAACGCA56500.031.2773365
AACGCAG57600.030.5919026
TGGTATC10150.029.0175612
TAACTCG1405.7977195E-727.145464
ACGCAGA66000.026.417047
CGCAGAG66150.026.3571368
GTGGTAT11900.025.9606551
ATACCCG1109.467098E-525.9115754
TATAGGG1858.951247E-925.6781392
GTACATG38750.025.5117861
GCAGAGT70500.024.3265259
TACATGG40050.024.0784652
ACCCGTA1000.001654083723.7478926
ACATGGG39600.023.3923953
TACCCGT1252.2536475E-422.8021875
Produced by FastQC (version 0.11.5)