FastQCFastQC Report
Thu 26 May 2016
SRR937149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907720
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.6206759793769003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.47228220155995243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.31011765742740055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14210.15654607147578548No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.11787775966156964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.056.7602271
GTATCAA44800.045.323891
ATCAACG56650.035.8311963
TCAACGC57200.035.4035534
TATCAAC57750.034.901752
CAACGCA58100.034.849365
AACGCAG59950.033.7552836
ACGCAGA67850.029.5457
CGCAGAG67600.029.5120938
GTACATG43850.027.4364721
TACATGG44600.026.5398082
GCAGAGT72750.026.182369
ACATGGG44500.025.53123
TGGTATC10050.025.0693552
GAGTACT43700.023.74889612-13
CATGGGG15800.022.5651114
AGTACTT45550.022.47151212-13
GTGGTAT11900.022.3778511
GTACTTT46150.022.2314314-15
CAGAGTA70400.021.86038410-11
Produced by FastQC (version 0.11.5)