##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937149_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 907720 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87324946018596 33.0 31.0 34.0 30.0 34.0 2 31.984894020182434 33.0 31.0 34.0 30.0 34.0 3 32.021382144273566 34.0 31.0 34.0 30.0 34.0 4 35.6393788833561 37.0 35.0 37.0 35.0 37.0 5 35.63546798572247 37.0 35.0 37.0 35.0 37.0 6 35.685600185079096 37.0 35.0 37.0 35.0 37.0 7 35.64041113999912 37.0 35.0 37.0 33.0 37.0 8 35.63340677742035 37.0 35.0 37.0 33.0 37.0 9 37.29064248887322 39.0 37.0 39.0 35.0 39.0 10-11 37.19915667827083 39.0 37.0 39.0 34.0 39.0 12-13 37.094464151941125 39.0 37.0 39.0 34.0 39.0 14-15 38.309045740977396 40.0 38.0 41.0 34.0 41.0 16-17 38.26075166350857 40.0 38.0 41.0 34.0 41.0 18-19 38.23739148636143 40.0 38.0 41.0 33.5 41.0 20-21 38.18215253602432 40.0 38.0 41.0 34.0 41.0 22-23 38.046527012735204 40.0 38.0 41.0 33.5 41.0 24-25 37.916931432600364 40.0 38.0 41.0 33.0 41.0 26-27 37.750268254527825 40.0 37.5 41.0 33.0 41.0 28-29 37.56438218834002 40.0 37.0 41.0 32.5 41.0 30-31 37.50789946238929 40.0 37.0 41.0 33.0 41.0 32-33 37.32328581500903 40.0 36.5 41.0 32.0 41.0 34-35 37.12000837262592 39.5 36.0 41.0 31.5 41.0 36-37 37.143040805534746 40.0 37.0 41.0 32.0 41.0 38-39 37.11949444762702 40.0 37.0 41.0 32.0 41.0 40-41 36.94667573701142 40.0 36.5 41.0 31.0 41.0 42-43 36.69602520601066 40.0 36.0 41.0 31.0 41.0 44-45 36.43450017626581 40.0 36.0 41.0 30.0 41.0 46-47 36.05937128189309 39.0 35.5 41.0 29.5 41.0 48-49 35.71021625611422 39.0 35.0 41.0 27.5 41.0 50-51 34.750889040673336 38.0 34.0 40.0 25.5 40.5 52-53 34.85135063676023 38.5 34.5 40.0 26.0 40.5 54-55 35.193003349050365 39.0 35.0 40.0 26.0 41.0 56-57 34.97684418102499 39.0 35.0 40.0 25.5 41.0 58-59 34.74764134314546 38.0 35.0 40.0 25.0 41.0 60-61 34.464692305997445 38.0 34.0 40.0 24.5 41.0 62-63 34.15317278455912 38.0 34.0 40.0 23.0 41.0 64-65 33.7878046093509 37.0 34.0 40.0 22.0 41.0 66-67 33.398685718062836 36.5 33.5 39.5 21.5 41.0 68-69 32.919394196448245 36.0 33.0 39.0 19.5 41.0 70-71 32.49156347772441 35.5 32.5 39.0 18.0 40.0 72-73 32.092785770942584 35.0 32.0 38.0 15.5 40.0 74-75 31.65183646939585 35.0 32.0 37.0 11.5 39.5 76-77 31.2239302868726 35.0 31.0 37.0 7.5 39.0 78-79 30.781834155907106 35.0 31.0 36.0 6.0 39.0 80-81 30.350941920415988 35.0 31.0 36.0 2.0 37.5 82-83 29.93549387476314 34.0 30.0 35.5 2.0 37.0 84-85 29.500234103027367 34.0 30.0 35.0 2.0 37.0 86-87 29.10064447186357 34.0 29.0 35.0 2.0 36.0 88-89 28.751742828184902 34.0 29.0 35.0 2.0 36.0 90-91 28.351057044022383 34.0 29.0 35.0 2.0 35.5 92-93 27.93341283655753 34.0 28.5 35.0 2.0 35.0 94-95 27.443525536509057 34.0 27.0 35.0 2.0 35.0 96-97 26.87577501872824 33.0 25.5 35.0 2.0 35.0 98-99 26.229824725686335 33.0 25.0 35.0 2.0 35.0 100-101 24.496210285109946 31.5 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08399443122127082 1101 2 0.15879119156941712 1101 3 0.23939970680860156 1101 4 0.11821228304181375 1101 5 0.3164336920896105 1101 6 0.1383342818081985 1101 7 0.25108571554942927 1101 8 0.20475402216324312 1101 9 0.12600330296187678 1101 10-11 0.15471287444221815 1101 12-13 0.23230506559352904 1101 14-15 0.2734032656095522 1101 16-17 0.2676237292645709 1101 18-19 0.31137312323240707 1101 20-21 0.34227895987451973 1101 22-23 0.29134579680816586 1101 24-25 0.30990194295088 1101 26-27 0.3912286835206231 1101 28-29 0.2387926375907412 1101 30-31 0.2291097284447119 1101 32-33 0.2664267089249037 1101 34-35 0.27671970686568415 1101 36-37 0.2675058511288242 1101 38-39 0.3510891125005031 1101 40-41 0.33638101915082785 1101 42-43 0.37856459399107223 1101 44-45 0.3484452998414014 1101 46-47 0.5554801442794144 1101 48-49 0.8280707253692228 1101 50-51 0.7383718261704999 1101 52-53 0.8578986212262265 1101 54-55 0.9028690274607172 1101 56-57 0.9919256634242686 1101 58-59 0.9363935070739657 1101 60-61 0.9511684270819671 1101 62-63 0.9126727145329312 1101 64-65 0.9524597989367152 1101 66-67 0.9269010619387359 1101 68-69 0.7581797676814261 1101 70-71 0.7396655648961641 1101 72-73 0.7103919087509212 1101 74-75 0.6601931887637527 1101 76-77 0.8393684435802662 1101 78-79 0.8810939432356868 1101 80-81 0.9267423740012397 1101 82-83 0.858926747818014 1101 84-85 0.7147729347713145 1101 86-87 0.8883644089674902 1101 88-89 0.8898950154597856 1101 90-91 0.8447474885504924 1101 92-93 0.686794406426916 1101 94-95 0.692971249571098 1101 96-97 0.9144786186850844 1101 98-99 0.9804548351404598 1101 100-101 1.38093694014675 1102 1 0.13594884132577434 1102 2 0.018564405445705034 1102 3 0.0759494709628612 1102 4 -0.04269529771069358 1102 5 0.031072463471687684 1102 6 0.1315015173063827 1102 7 0.16931939551371755 1102 8 0.20499953828272055 1102 9 0.2522330042562544 1102 10-11 0.0902274476295375 1102 12-13 0.25335364967079954 1102 14-15 0.3105922926628537 1102 16-17 0.3275455373490104 1102 18-19 0.28645529721099905 1102 20-21 0.27320398123902834 1102 22-23 0.4157460031095681 1102 24-25 0.3269718571196094 1102 26-27 0.5310086690853453 1102 28-29 0.4033621167136303 1102 30-31 0.19181769610929678 1102 32-33 0.20258888237998463 1102 34-35 0.2558155725487339 1102 36-37 0.3377021331471113 1102 38-39 0.5483376838778327 1102 40-41 0.5420649960369346 1102 42-43 0.4066544979707558 1102 44-45 0.5699677324529233 1102 46-47 0.37324247207276073 1102 48-49 0.326855263040315 1102 50-51 0.43610173517199513 1102 52-53 0.4431946293231732 1102 54-55 0.6108593532046172 1102 56-57 0.6452941058050499 1102 58-59 0.5368950337334226 1102 60-61 0.517622708975189 1102 62-63 0.5544908716739201 1102 64-65 0.5453065789431264 1102 66-67 0.6740800502184285 1102 68-69 0.6834945154687517 1102 70-71 0.4446109333181809 1102 72-73 0.5005402169679343 1102 74-75 0.5134025425158804 1102 76-77 0.5941236521332449 1102 78-79 0.5855257394393725 1102 80-81 0.5885851215326277 1102 82-83 0.5887843678579436 1102 84-85 0.6260027402827966 1102 86-87 0.6218149744301371 1102 88-89 0.6466525255143125 1102 90-91 0.5195552079844639 1102 92-93 0.38467312776539586 1102 94-95 0.38835639480370077 1102 96-97 0.6653500433717419 1102 98-99 0.5973008764172185 1102 100-101 0.9207101879541923 1103 1 0.02442926851766103 1103 2 0.044686521292245374 1103 3 -0.019379435064227124 1103 4 -0.005214265674105434 1103 5 0.004786944261006454 1103 6 0.021983145218705147 1103 7 0.0552913147997387 1103 8 0.01729055434894633 1103 9 0.09663544403944968 1103 10-11 0.009521881432668522 1103 12-13 0.05104585042542453 1103 14-15 -0.02436106030130958 1103 16-17 0.022124890002615416 1103 18-19 0.06121959556592316 1103 20-21 -0.0714396396674104 1103 22-23 -0.004563185204538911 1103 24-25 -0.024391762172420783 1103 26-27 0.002407334615192269 1103 28-29 -0.09162058719442001 1103 30-31 -0.10165838954209505 1103 32-33 -0.16680642427179748 1103 34-35 -0.16859944060481524 1103 36-37 -0.056385860265180554 1103 38-39 0.03883310157662834 1103 40-41 -0.010675191492957481 1103 42-43 -0.12838669481214282 1103 44-45 -0.1885994526760726 1103 46-47 -0.2328046295775792 1103 48-49 -0.12598310664817092 1103 50-51 -0.24054851267980126 1103 52-53 -0.20130845908449402 1103 54-55 -0.23114346753543913 1103 56-57 -0.12754424706661638 1103 58-59 -0.1684711831956065 1103 60-61 -0.09740078691505971 1103 62-63 -0.0706920812350873 1103 64-65 -0.04510761354995907 1103 66-67 0.18071960818289767 1103 68-69 0.07765636271716403 1103 70-71 0.015965230490330384 1103 72-73 0.08041769527564213 1103 74-75 0.285814028623232 1103 76-77 0.3169232983075041 1103 78-79 0.37298140611668984 1103 80-81 0.254492368962314 1103 82-83 0.2723634228539673 1103 84-85 0.2750675292962619 1103 86-87 0.4669056422762239 1103 88-89 0.37514564379424087 1103 90-91 0.338216152450304 1103 92-93 0.41487247986965414 1103 94-95 0.49436972268838275 1103 96-97 0.7393372928642918 1103 98-99 0.8921859306990889 1103 100-101 1.1098265989772749 1104 1 0.14384685906648542 1104 2 0.047470116396549855 1104 3 0.16267654593238845 1104 4 0.05128654002998445 1104 5 0.2901272465247544 1104 6 0.22664558852375905 1104 7 0.12699182661981467 1104 8 0.22315483812703008 1104 9 0.2606062551893231 1104 10-11 0.10192335406228636 1104 12-13 0.23046441081938696 1104 14-15 0.3047105362486988 1104 16-17 0.21463042910918517 1104 18-19 0.17308931396545546 1104 20-21 0.16563673773072907 1104 22-23 0.32508334112762327 1104 24-25 0.22005979912127316 1104 26-27 0.2647811457263387 1104 28-29 0.2016077307487265 1104 30-31 0.0801319306358792 1104 32-33 0.033511252342059095 1104 34-35 0.1570490910042892 1104 36-37 0.18680664666938895 1104 38-39 0.36231570384558154 1104 40-41 0.34264253361316577 1104 42-43 0.33774966192621036 1104 44-45 0.6412358097071547 1104 46-47 0.6640961221221673 1104 48-49 0.8689740474773231 1104 50-51 0.6294639801815478 1104 52-53 0.7034658303905559 1104 54-55 0.8440929671044941 1104 56-57 0.8275336074266164 1104 58-59 0.7838298790639158 1104 60-61 0.9577607254433076 1104 62-63 0.9986012609873285 1104 64-65 0.9546623098776195 1104 66-67 0.9224768838530082 1104 68-69 1.045871258073312 1104 70-71 0.9134771939488004 1104 72-73 0.7212576997829814 1104 74-75 0.7970377185211781 1104 76-77 0.7204924330905094 1104 78-79 0.633959756591512 1104 80-81 0.9338156891678544 1104 82-83 1.011419784462472 1104 84-85 1.0352729690111424 1104 86-87 0.9716308464233947 1104 88-89 1.0298992630086232 1104 90-91 1.1135072193741529 1104 92-93 1.1082425068404227 1104 94-95 1.0659380146131667 1104 96-97 1.2200098141860636 1104 98-99 1.3434130115663834 1104 100-101 1.5696962908630567 1105 1 -0.08911101827440149 1105 2 -0.07634722261304816 1105 3 -0.19260598533657003 1105 4 -0.1455701901265698 1105 5 -0.14639238558422107 1105 6 -0.21728415881136698 1105 7 -0.1636356088304396 1105 8 -0.22379201162203088 1105 9 0.09580671416460262 1105 10-11 -0.007156575669824861 1105 12-13 0.04922629454734562 1105 14-15 0.027116805526041787 1105 16-17 -3.723887146946936E-4 1105 18-19 0.025920211531207826 1105 20-21 0.10989332724995649 1105 22-23 0.07914792514320368 1105 24-25 0.0626259481714655 1105 26-27 0.03167507877383002 1105 28-29 0.08879481902276609 1105 30-31 0.011949795131556584 1105 32-33 0.10763493450168227 1105 34-35 0.20952788751424833 1105 36-37 0.1824434621730049 1105 38-39 0.26633421263287005 1105 40-41 0.3347672235890755 1105 42-43 0.21758280105816397 1105 44-45 -0.009537441782356382 1105 46-47 -0.08716555217292665 1105 48-49 -0.06994454279838891 1105 50-51 0.1605441333086972 1105 52-53 0.1398150603122872 1105 54-55 0.2479077548411226 1105 56-57 0.3839554534312697 1105 58-59 0.3808261676598548 1105 60-61 0.5107551710301124 1105 62-63 0.42117171293074307 1105 64-65 0.17879195770041179 1105 66-67 0.32074677807037233 1105 68-69 0.41487481462398534 1105 70-71 0.4127905080396914 1105 72-73 0.4615993545817858 1105 74-75 0.3591488397866094 1105 76-77 0.43910003461467184 1105 78-79 0.6190935143754324 1105 80-81 0.4772666603016589 1105 82-83 0.6834609424611564 1105 84-85 0.5008456980608749 1105 86-87 0.15627605447271975 1105 88-89 0.4169023042275448 1105 90-91 0.5525952598695802 1105 92-93 0.45511472233540573 1105 94-95 0.4725157294341571 1105 96-97 0.32095645069439627 1105 98-99 0.5050523377256901 1105 100-101 0.7579389841949684 1106 1 0.04155946800039345 1106 2 0.10123735874097761 1106 3 0.008861723261173182 1106 4 0.0014590371280220893 1106 5 0.06101324229408789 1106 6 0.08147477073136855 1106 7 0.07078075263019912 1106 8 0.22441210775280496 1106 9 0.12441751301872017 1106 10-11 0.016742416243722857 1106 12-13 -0.045612701717651305 1106 14-15 -0.08579431606079169 1106 16-17 -0.3508138798123497 1106 18-19 0.02480515188005228 1106 20-21 -0.01147366335816713 1106 22-23 0.04758176493723454 1106 24-25 -0.10262725195907763 1106 26-27 0.052906539773942995 1106 28-29 0.0962942750912319 1106 30-31 0.23678389601272443 1106 32-33 0.1956430572126422 1106 34-35 0.17198754592524068 1106 36-37 -0.09981621074283709 1106 38-39 -0.09730215100348971 1106 40-41 0.16265595332762217 1106 42-43 -0.055513891838081975 1106 44-45 0.019483572106025804 1106 46-47 0.058182020079307506 1106 48-49 0.3421357233654376 1106 50-51 0.03734817290691694 1106 52-53 0.28518982914138746 1106 54-55 0.19414238877519807 1106 56-57 0.20659524383631833 1106 58-59 -0.13779442196193514 1106 60-61 0.14146645217019937 1106 62-63 0.4570575960942449 1106 64-65 0.27529221153513106 1106 66-67 0.20013902523286475 1106 68-69 0.13521694756086333 1106 70-71 0.018995407049736457 1106 72-73 0.07485723252354859 1106 74-75 0.16507633371270813 1106 76-77 0.01261464824197489 1106 78-79 0.1499820265413696 1106 80-81 0.1490039776313168 1106 82-83 -0.23751260433508392 1106 84-85 -0.01914831869221345 1106 86-87 0.02268873532995741 1106 88-89 0.26208352408467306 1106 90-91 0.12409052766520645 1106 92-93 0.29705501678367696 1106 94-95 0.36016893681712503 1106 96-97 0.0795822983210428 1106 98-99 0.1585203545513032 1106 100-101 0.047330959391690186 1107 1 0.20481872140121382 1107 2 0.06407546377103301 1107 3 0.24382494692457257 1107 4 0.2429273224807602 1107 5 0.2912311322023413 1107 6 0.26343854801703515 1107 7 0.13318189151029713 1107 8 0.3185575522323987 1107 9 0.18801487144959594 1107 10-11 0.305886476667645 1107 12-13 0.3854118949289713 1107 14-15 0.46561346628656963 1107 16-17 0.48265456371817805 1107 18-19 0.4279855141071849 1107 20-21 0.4677605584071003 1107 22-23 0.3149071761994904 1107 24-25 0.30728060947717495 1107 26-27 0.3827616093721673 1107 28-29 0.30757037442688073 1107 30-31 0.22502436580076335 1107 32-33 0.33160675540941753 1107 34-35 0.29709135010114096 1107 36-37 0.2810660258911426 1107 38-39 0.3191849179620263 1107 40-41 0.3008786281140843 1107 42-43 0.3318239759252819 1107 44-45 0.38882871604781855 1107 46-47 0.28228040666584064 1107 48-49 0.5381377796767381 1107 50-51 0.45509572115114594 1107 52-53 0.3619703179107674 1107 54-55 0.31975038466257644 1107 56-57 0.6096075421596296 1107 58-59 0.6242073179937719 1107 60-61 0.43315618643150344 1107 62-63 0.47976599533499353 1107 64-65 0.6136938249485979 1107 66-67 0.7194515450050858 1107 68-69 0.5647487412970165 1107 70-71 0.6605991321367313 1107 72-73 0.6188800892144712 1107 74-75 0.7078139958449476 1107 76-77 0.539059997814892 1107 78-79 0.8014866927862272 1107 80-81 0.8142849409340691 1107 82-83 0.8423474083191387 1107 84-85 0.9682370024087028 1107 86-87 0.8452923417836118 1107 88-89 1.0861898195607864 1107 90-91 1.057774488437115 1107 92-93 1.0606138193777959 1107 94-95 0.8963521067000713 1107 96-97 1.016444644097696 1107 98-99 0.9956713747980608 1107 100-101 1.222928080555878 1108 1 -0.013856474302997412 1108 2 -0.048124914698270516 1108 3 -0.14403128326240022 1108 4 -0.020652689811406333 1108 5 0.0844417325775737 1108 6 0.09737705484242554 1108 7 0.00789884822894038 1108 8 0.05591158276872932 1108 9 0.05190946613161174 1108 10-11 -0.07527072557082448 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11 2108.0 12 2751.0 13 3849.0 14 4553.0 15 5833.0 16 7747.0 17 10811.0 18 8993.0 19 5616.0 20 5149.0 21 5532.0 22 5797.0 23 6273.0 24 6886.0 25 7630.0 26 8787.0 27 10331.0 28 12120.0 29 15007.0 30 18392.0 31 23365.0 32 30020.0 33 40410.0 34 56749.0 35 83100.0 36 127281.0 37 188692.0 38 166744.0 39 25807.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58497052447966 19.22104595262914 15.044508975304232 22.149474547586966 2 15.149784033865082 19.975292899581547 40.89168765186414 23.983235414689233 3 18.669687007928395 25.243017762921628 30.30050553882704 25.786789690322937 4 12.345852265695443 16.542427644490022 37.59679814713318 33.514921942681354 5 13.103457419481007 37.5514447622741 34.52038842245638 14.824709395788519 6 31.142948942310984 36.40980853504633 17.73985012713909 14.707392395503593 7 27.778544588445353 33.38714503874112 20.98965688964662 17.844653483166912 8 26.65993098688751 34.21747412008281 19.98288474810214 19.139710144927534 9 26.53190793977484 16.410759792771746 20.613514002923807 36.44381826452961 10-11 24.46877410428012 26.97222358723037 27.314328370899894 21.24467393758962 12-13 25.682617505617856 24.340240394436808 28.221887267487123 21.755254832458217 14-15 22.72128056105144 25.360869930045222 26.80164464386659 25.116204865036746 16-17 21.370683006090292 28.89579145371438 27.475275266764314 22.258250273431006 18-19 21.395595071629632 26.72645601282052 30.794310259452125 21.083638656097726 20-21 22.182671340638716 25.829694443677713 30.905738496784345 21.081895718899226 22-23 21.825426618145848 25.780421936828716 30.9096660366026 21.484485408422838 24-25 21.48608144136983 26.152427950828265 30.958042845866384 21.403447761935514 26-27 21.131060494809983 26.41183816376848 30.776178367438423 21.68092297398311 28-29 21.21893508110997 26.479986397021477 30.849722419740218 21.451356102128337 30-31 21.361998857133393 26.28140467691934 30.767294139081898 21.58930232686537 32-33 21.003111776605802 26.40714582284296 30.67054479279852 21.919197607752718 34-35 21.54293654346927 26.32634235181175 30.57194071641781 21.55878038830117 36-37 21.59808244239074 26.222114513563838 30.33959693530838 21.840206108737043 38-39 21.279629407806524 26.462654015512367 30.592789636931595 21.664926939749513 40-41 21.566534206733543 26.499530529907812 30.408634937737943 21.5253003256207 42-43 21.201912753659826 26.559080253186494 30.237482398438093 22.001524594715587 44-45 21.496006611573897 26.664749148296124 30.03788846999148 21.801355770138496 46-47 21.702466856855178 26.939679918036408 29.49690207646991 21.860951148638502 48-49 21.52492461390505 27.0828479657624 29.25515998635266 22.13706743397989 50-51 21.755103325325845 27.269449724798907 29.096118033083762 21.87932891679149 52-53 21.813267514798127 27.317011220072445 28.81405159466384 22.055669670465587 54-55 21.6301007942621 27.2767328413712 28.932121924891575 22.16104443947513 56-57 21.6704217920172 27.483819475798484 28.79206025568706 22.053698476497257 58-59 21.698447993915824 27.135077677570614 28.951334859896694 22.215139468616872 60-61 21.590624334208727 27.073156730991073 29.208656362839548 22.127562571960652 62-63 21.41657453806452 27.298969686700197 29.431293772584848 21.853162002650432 64-65 21.28464338029537 27.18170368105406 29.24775004994983 22.285902888700743 66-67 21.313503326897028 27.332719510619906 28.933626555118742 22.420150607364327 68-69 21.45645638186955 26.970974968829015 29.12051732521756 22.45205132408387 70-71 21.33902160479687 27.080650511872673 29.162584362258038 22.41774352107242 72-73 21.390953826246424 26.960433064563322 29.034282805822038 22.61433030336822 74-75 21.37219477972497 27.410556134212307 28.677510822905095 22.539738263157634 76-77 21.40625750418578 27.08462485833477 28.81375487237295 22.695362765106495 78-79 21.362348905595425 27.06050621214187 28.813993451954783 22.76315143030792 80-81 21.35832302092912 27.07378677002253 28.847667483195654 22.72022272585269 82-83 21.42614248617711 26.989248617710963 28.80355817979473 22.781050716317193 84-85 21.31086014158155 26.923841136361375 28.860128335185987 22.905170386871088 86-87 21.44039104560322 26.85706369172925 28.743242750963425 22.959302511704102 88-89 21.20580169661428 26.698159866814454 28.779515215212122 23.316523221359144 90-91 21.12489705619352 26.838416310273182 28.90783521042487 23.12885142310843 92-93 21.155241034020214 26.79024271764271 28.903382149185514 23.151134099151566 94-95 21.013909949375712 26.596050632017004 28.90535946225976 23.48467995634752 96-97 21.17134968830759 26.87437697395075 28.502717304310483 23.451556033431178 98-99 21.15160842310724 27.134812109596663 28.248420722592556 23.465158744703544 100-101 21.351226794934416 26.83108561210044 28.303957038030504 23.51373055493464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1343.0 1 787.5 2 247.0 3 276.0 4 257.0 5 252.0 6 430.5 7 906.0 8 1519.0 9 1825.5 10 1692.5 11 1514.5 12 1555.0 13 1652.5 14 1711.0 15 1745.5 16 1780.5 17 1829.0 18 1936.5 19 2083.5 20 2249.5 21 2561.5 22 2965.0 23 3444.5 24 4081.0 25 4900.5 26 6127.5 27 7817.0 28 9027.5 29 10620.0 30 12554.5 31 13831.0 32 15309.5 33 16603.5 34 18188.0 35 19791.0 36 22086.5 37 24534.0 38 25323.0 39 25660.0 40 26203.0 41 27206.5 42 28316.5 43 29658.0 44 31072.0 45 32114.0 46 32791.5 47 33532.0 48 34651.5 49 34928.5 50 33695.5 51 31941.5 52 30661.5 53 30247.0 54 29594.5 55 27277.0 56 24184.5 57 21333.5 58 19360.0 59 17131.0 60 14176.5 61 11554.0 62 9136.0 63 6734.0 64 4712.5 65 3400.5 66 2525.0 67 1918.5 68 1430.5 69 1042.5 70 745.5 71 525.0 72 392.0 73 317.0 74 238.5 75 176.0 76 124.0 77 86.0 78 62.0 79 48.0 80 33.5 81 21.0 82 14.0 83 12.5 84 10.0 85 7.0 86 5.0 87 4.0 88 4.5 89 4.5 90 3.5 91 1.5 92 2.0 93 2.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33832018684175735 2 0.29932137663596703 3 0.3024060282906623 4 0.3022958621601375 5 0.28896576036663285 6 0.2224254175296347 7 0.2305777111884722 8 0.23079804344952187 9 0.22606089983695415 10-11 0.23680209756312517 12-13 0.23410302736526684 14-15 0.21945093200546423 16-17 0.23118362490635877 18-19 0.21862468602652801 20-21 0.21978143039703874 22-23 0.21945093200546423 24-25 0.2109681399550522 26-27 0.22462874014013132 28-29 0.22429824174855684 30-31 0.23162428942845814 32-33 0.23443352575684132 34-35 0.22093817476754948 36-37 0.21862468602652801 38-39 0.21223505045608776 40-41 0.2105825584982153 42-43 0.20992156171506632 44-45 0.22424315868329442 46-47 0.215044286784471 48-49 0.22573040144537962 50-51 0.23239545234213194 52-53 0.24236548715462916 54-55 0.23823425725994798 56-57 0.20347684307936367 58-59 0.19427797118054024 60-61 0.20303617855726436 62-63 0.20165910192570394 64-65 0.19951086238046972 66-67 0.21162913673820122 68-69 0.2015489357951791 70-71 0.2070572423214207 72-73 0.21912043361388975 74-75 0.20782840523509452 76-77 0.21763319085180452 78-79 0.21520953598025824 80-81 0.2041378398625127 82-83 0.21768827391706694 84-85 0.19951086238046972 86-87 0.20281584629621469 88-89 0.21107830608557707 90-91 0.20777332216983213 92-93 0.21317146256554886 94-95 0.21256554884766227 96-97 0.20479883664566165 98-99 0.21217996739082537 100-101 0.1985744502710087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 907720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.237975631909755 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4127030903309 47.042477705291056 2 11.562321427804248 13.69857029977152 3 3.7367880612688893 6.640792803451734 4 1.6852747912288881 3.993290680635547 5 0.9070760682547535 2.6866675013781802 6 0.5519887893179557 1.9619219070422658 7 0.3812674962742697 1.5809860257474102 8 0.27062990453566815 1.282525415211998 9 0.2111112534222785 1.1255222957265771 >10 1.1832826875598799 12.662111399280132 >50 0.06838514578904636 2.7454771234958866 >100 0.0280564581012425 2.8259635262603444 >500 1.8580435198916072E-4 0.07770711614858299 >1k 7.432174079566429E-4 1.0558702056846412 >5k 1.8580435198916072E-4 0.6201159948741028 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5634 0.6206759793769003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4287 0.47228220155995243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2815 0.31011765742740055 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1421 0.15654607147578548 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1070 0.11787775966156964 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1016613052483145E-4 0.0 12-13 0.0 0.0 0.0 1.1016613052483145E-4 0.0 14-15 0.0 0.0 0.0 1.1016613052483145E-4 0.0 16-17 0.0 0.0 0.0 2.203322610496629E-4 0.0 18-19 0.0 0.0 0.0 3.304983915744943E-4 0.0 20-21 0.0 0.0 0.0 3.304983915744943E-4 0.0 22-23 0.0 0.0 0.0 4.406645220993258E-4 0.0 24-25 0.0 0.0 0.0 6.609967831489887E-4 0.0 26-27 0.0 0.0 0.0 7.160798484114044E-4 0.0 28-29 5.5083065262415725E-5 0.0 0.0 0.002093156479971797 0.0 30-31 2.203322610496629E-4 0.0 0.0 0.005563389591503988 0.0 32-33 2.203322610496629E-4 0.0 0.0 0.009584453355660336 0.0 34-35 2.203322610496629E-4 0.0 0.0 0.015037676816639492 0.0 36-37 2.203322610496629E-4 0.0 0.0 0.024787379368087073 0.0 38-39 2.203322610496629E-4 0.0 0.0 0.036960736791080945 0.0 40-41 2.203322610496629E-4 0.0 0.0 0.05712113867712511 0.0 42-43 2.203322610496629E-4 0.0 0.0 0.0851584188956947 0.0 44-45 2.203322610496629E-4 0.0 0.0 0.11479310800687437 0.0 46-47 2.203322610496629E-4 0.0 0.0 0.16750760146300622 0.0 48-49 2.203322610496629E-4 0.0 0.0 0.2329462829947561 0.0 50-51 2.203322610496629E-4 0.0 0.0 0.28119904816463226 0.0 52-53 2.203322610496629E-4 0.0 0.0 0.351650288635262 0.0 54-55 2.203322610496629E-4 0.0 0.0 0.4440796721455955 0.0 56-57 3.304983915744943E-4 0.0 0.0 0.5892786321773233 0.0 58-59 3.304983915744943E-4 0.0 0.0 0.754197329572996 0.0 60-61 3.304983915744943E-4 0.0 0.0 0.9412043361388974 0.0 62-63 3.304983915744943E-4 0.0 0.0 1.1658330762790288 0.0 64-65 3.304983915744943E-4 0.0 0.0 1.4158000264398714 0.0 66-67 3.304983915744943E-4 0.0 0.0 1.6926475124487728 0.0 68-69 3.304983915744943E-4 0.0 0.0 2.0375225840567577 0.0 70-71 3.304983915744943E-4 0.0 0.0 2.379698585466884 0.0 72-73 3.8558145683691006E-4 1.1016613052483145E-4 0.0 2.7463865509187855 0.0 74-75 4.406645220993258E-4 1.1016613052483145E-4 0.0 3.144306614374477 0.0 76-77 4.406645220993258E-4 1.1016613052483145E-4 0.0 3.626118186224827 0.0 78-79 4.406645220993258E-4 1.1016613052483145E-4 0.0 4.171660864583792 0.0 80-81 4.406645220993258E-4 1.1016613052483145E-4 0.0 4.830289075926498 0.0 82-83 4.406645220993258E-4 1.1016613052483145E-4 0.0 5.515522407790948 0.0 84-85 4.406645220993258E-4 1.1016613052483145E-4 0.0 6.291202132816287 0.0 86-87 4.406645220993258E-4 1.1016613052483145E-4 0.0 7.076025646675186 0.0 88-89 4.406645220993258E-4 1.1016613052483145E-4 0.0 8.094070858855154 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2195 0.0 56.760227 1 GTATCAA 4480 0.0 45.32389 1 ATCAACG 5665 0.0 35.831196 3 TCAACGC 5720 0.0 35.403553 4 TATCAAC 5775 0.0 34.90175 2 CAACGCA 5810 0.0 34.84936 5 AACGCAG 5995 0.0 33.755283 6 ACGCAGA 6785 0.0 29.545 7 CGCAGAG 6760 0.0 29.512093 8 GTACATG 4385 0.0 27.436472 1 TACATGG 4460 0.0 26.539808 2 GCAGAGT 7275 0.0 26.18236 9 ACATGGG 4450 0.0 25.5312 3 TGGTATC 1005 0.0 25.069355 2 GAGTACT 4370 0.0 23.748896 12-13 CATGGGG 1580 0.0 22.565111 4 AGTACTT 4555 0.0 22.471512 12-13 GTGGTAT 1190 0.0 22.377851 1 GTACTTT 4615 0.0 22.23143 14-15 CAGAGTA 7040 0.0 21.860384 10-11 >>END_MODULE