FastQCFastQC Report
Thu 26 May 2016
SRR937149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907720
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59470.6551579782311726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48440.5336447362622836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34160.3763275018728242No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.1398008196360111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.054.658671
GTATCAA49400.042.999871
TCAACGC63600.033.014764
ATCAACG63750.032.9370773
TATCAAC64750.032.64852
CAACGCA64900.032.3534475
AACGCAG66400.031.545816
TGGTATC12050.029.1734432
ATACCGT1056.9066904E-527.1414556
ACGCAGA76750.026.9838077
CGCAGAG76900.026.68418
GTGGTAT13950.026.570941
GCAGAGT81600.024.5650359
GGTAAGG4000.023.75273
TAAGGTG4600.023.7526995
AGTACTT53000.022.80904212-13
ATCACGC1252.255342E-422.800088
GAGTACT51500.022.45881712-13
GTAAGGT4400.021.5933634
AGAGTAC75700.021.5852810-11
Produced by FastQC (version 0.11.5)