FastQCFastQC Report
Thu 26 May 2016
SRR937148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366774
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76840.5621997491904295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56440.4129431786088995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.275173510763301No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20070.14684212605741695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.10843050862834676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.056.9201131
GTATCAA63750.042.641921
ATCAACG81450.033.3680043
TCAACGC81800.033.168364
TATCAAC83250.032.7036062
CAACGCA83400.032.533235
AACGCAG85350.031.8374376
TATGCCG700.00837670827.153085
ACGCAGA98250.027.077037
CGCAGAG98900.026.610878
TAAACCG908.928754E-426.3988275
GTACATG68200.025.9226531
TACATGG69650.025.172742
ACATGGG69400.024.2364543
TGGTATC13600.024.1065712
GCAGAGT106900.024.0416989
GAGTACT61500.023.82877712-13
GTACTTT65650.022.174914-15
CAGAGTA104000.021.26180310-11
AGTACTT63950.021.05882812-13
Produced by FastQC (version 0.11.5)