##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1366774 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.908382805057748 33.0 31.0 34.0 30.0 34.0 2 32.01373453109292 33.0 31.0 34.0 30.0 34.0 3 32.05243222361561 34.0 31.0 34.0 30.0 34.0 4 35.663938588237706 37.0 35.0 37.0 35.0 37.0 5 35.67992660088647 37.0 35.0 37.0 35.0 37.0 6 35.70653085294277 37.0 35.0 37.0 35.0 37.0 7 35.58744093756539 37.0 35.0 37.0 33.0 37.0 8 35.60234976667686 37.0 35.0 37.0 33.0 37.0 9 37.25993031766773 39.0 37.0 39.0 34.0 39.0 10-11 37.15564131304809 39.0 37.0 39.0 34.0 39.0 12-13 37.01901155567782 39.0 37.0 39.0 33.0 39.0 14-15 38.229572336026294 40.0 38.0 41.0 33.5 41.0 16-17 38.168349705218276 40.0 38.0 41.0 33.0 41.0 18-19 38.15167211258043 40.0 38.0 41.0 33.5 41.0 20-21 38.04014160351309 40.0 38.0 41.0 33.0 41.0 22-23 37.89321533772225 40.0 38.0 41.0 33.0 41.0 24-25 37.8257564893684 40.0 38.0 41.0 33.0 41.0 26-27 37.83309493742199 40.0 38.0 41.0 33.0 41.0 28-29 37.74088254532205 40.0 38.0 41.0 33.0 41.0 30-31 37.515605725599116 40.0 37.0 41.0 32.5 41.0 32-33 37.265204049828284 39.5 36.5 41.0 32.0 41.0 34-35 37.318363899225474 40.0 37.0 41.0 32.0 41.0 36-37 37.20837680552893 40.0 37.0 41.0 32.0 41.0 38-39 37.0852694007934 40.0 36.5 41.0 31.0 41.0 40-41 36.868988947697275 39.5 36.0 41.0 31.0 41.0 42-43 36.58720278553733 39.0 36.0 41.0 30.0 41.0 44-45 36.323658483406916 39.0 36.0 41.0 30.0 41.0 46-47 35.95199572131165 39.0 35.0 41.0 28.5 41.0 48-49 35.59250651534197 39.0 35.0 41.0 27.5 41.0 50-51 34.59502887821981 38.0 34.0 39.5 25.5 40.5 52-53 34.694002812462045 38.0 34.5 39.5 26.0 40.5 54-55 35.0558259814717 39.0 35.0 40.0 26.0 41.0 56-57 34.83664965824635 38.5 34.5 40.0 25.5 41.0 58-59 34.57280281890056 38.0 34.0 40.0 25.0 41.0 60-61 34.26485505284707 38.0 34.0 40.0 23.5 41.0 62-63 33.94409939024301 37.5 34.0 40.0 23.0 41.0 64-65 33.509764964800326 37.0 33.0 40.0 22.0 41.0 66-67 33.149799820599455 36.0 33.0 39.0 20.5 41.0 68-69 32.68196863563398 36.0 32.5 39.0 19.0 40.5 70-71 32.21009142696598 35.0 32.0 38.5 17.5 40.0 72-73 31.759442307213924 35.0 31.5 38.0 14.0 40.0 74-75 31.283568827033584 35.0 31.0 37.0 9.0 39.0 76-77 30.81689072224084 35.0 31.0 36.5 7.0 39.0 78-79 30.329336452112784 34.5 30.0 36.0 4.0 38.5 80-81 29.855445011391787 34.0 30.0 35.5 2.0 37.5 82-83 29.394069538928896 34.0 29.0 35.0 2.0 37.0 84-85 28.891160864927194 34.0 29.0 35.0 2.0 36.5 86-87 28.451044210674187 34.0 29.0 35.0 2.0 36.0 88-89 28.01715682329339 34.0 27.5 35.0 2.0 36.0 90-91 27.537604241813206 33.0 26.5 35.0 2.0 35.0 92-93 27.045755918681508 33.0 25.5 35.0 2.0 35.0 94-95 26.51017761531899 33.0 25.0 35.0 2.0 35.0 96-97 25.81231717899228 32.5 23.5 35.0 2.0 35.0 98-99 24.922170014940292 32.0 17.5 34.0 2.0 35.0 100-101 22.431564764913585 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10254290225028839 1101 2 0.023338294484879896 1101 3 -0.02259707226962604 1101 4 -0.0244706260951304 1101 5 -0.04968963499620571 1101 6 -0.11955304295626235 1101 7 -0.016588052467497505 1101 8 -0.1350865844175928 1101 9 -0.053677296633757976 1101 10-11 -0.08457313674943379 1101 12-13 -0.055584547839863774 1101 14-15 -0.12473333601716519 1101 16-17 -0.17132822676168047 1101 18-19 0.0871344600161379 1101 20-21 0.05664126473620712 1101 22-23 0.0700019213644012 1101 24-25 0.02654123232878902 1101 26-27 -0.09609415793073595 1101 28-29 -0.1378758782675078 1101 30-31 -0.07944926364842786 1101 32-33 -0.23762021410748702 1101 34-35 -0.22519721800678383 1101 36-37 -0.11825613798468737 1101 38-39 -0.07887627724743851 1101 40-41 -0.13754402895373374 1101 42-43 -0.12700031630012631 1101 44-45 -0.059620694330227764 1101 46-47 -0.2167253554465205 1101 48-49 -0.3353059298049885 1101 50-51 -0.23035780051177568 1101 52-53 -0.21465910451061632 1101 54-55 -0.2819173314795478 1101 56-57 -0.15777229119934333 1101 58-59 -0.19791587634706076 1101 60-61 -0.16015199611608466 1101 62-63 -0.20188017983223716 1101 64-65 -0.23708485133340673 1101 66-67 -0.30588999135588324 1101 68-69 -0.09235099005176295 1101 70-71 -0.2871157327125431 1101 72-73 -0.2690097336534194 1101 74-75 -0.3343759387440315 1101 76-77 -0.23205647218411585 1101 78-79 -0.28806143984184374 1101 80-81 -0.15771879846435155 1101 82-83 -0.2993360644941738 1101 84-85 -0.28543050389673397 1101 86-87 -0.20387774759172927 1101 88-89 -0.37120882855160886 1101 90-91 -0.2990041651129296 1101 92-93 -0.35893015913569926 1101 94-95 -0.4419384258981722 1101 96-97 -0.2824085602680384 1101 98-99 0.11659732881505036 1101 100-101 0.34762479534499846 1102 1 -0.07057737722117352 1102 2 -0.07428506596538043 1102 3 -0.11472076426878175 1102 4 -0.19508002914538025 1102 5 -0.1616884705317787 1102 6 -0.2811467419098648 1102 7 -0.1695444982632992 1102 8 -0.13913228964636204 1102 9 -0.256084665105881 1102 10-11 -0.17429956847461625 1102 12-13 -0.08579593047964096 1102 14-15 -0.037463600771090455 1102 16-17 -0.05068647747733479 1102 18-19 -0.20224303670880062 1102 20-21 -0.1929936271465067 1102 22-23 -0.231912167037855 1102 24-25 -0.19118773857577054 1102 26-27 -0.4057972195017925 1102 28-29 -0.40292051607055157 1102 30-31 -0.35599217901450686 1102 32-33 -0.40871359768529913 1102 34-35 -0.283114767456091 1102 36-37 -0.34675155290600657 1102 38-39 -0.141026735755311 1102 40-41 -0.15800180903168837 1102 42-43 -0.16446008567147885 1102 44-45 -0.05526567823797279 1102 46-47 -0.11019384212796268 1102 48-49 -0.24319275033191445 1102 50-51 -0.24903653771479384 1102 52-53 -0.23626558022668576 1102 54-55 -0.1067992677251226 1102 56-57 -0.12170543153347779 1102 58-59 -0.25053066504529653 1102 60-61 0.03943437641549252 1102 62-63 -0.059155252206942066 1102 64-65 -0.11328206632265392 1102 66-67 -0.3367426380864842 1102 68-69 -0.17690808924149337 1102 70-71 -0.24899286554235545 1102 72-73 -0.21253927385170002 1102 74-75 -0.241110424681505 1102 76-77 -0.1549960170726017 1102 78-79 -0.22558792332100452 1102 80-81 -0.21240212883261123 1102 82-83 -0.22444887198556174 1102 84-85 -0.1538254028956203 1102 86-87 -0.23646091784613432 1102 88-89 -0.37421217638683757 1102 90-91 -0.549681818717108 1102 92-93 -0.4090672263026498 1102 94-95 -0.5610789055928223 1102 96-97 -0.5409419011488268 1102 98-99 -0.36736662130705966 1102 100-101 -0.21376673964553916 1103 1 -0.08545987250016296 1103 2 -0.12782133697657372 1103 3 -0.16687950514089422 1103 4 -0.01821298575515584 1103 5 -0.10389180072974114 1103 6 -0.11919701799030946 1103 7 -0.1589058872100253 1103 8 -0.23719111021920725 1103 9 -0.25331991149601407 1103 10-11 -0.12487273158826184 1103 12-13 -0.0025959103749571 1103 14-15 -0.2153461104105716 1103 16-17 -0.28830342572931045 1103 18-19 -0.19857608522572434 1103 20-21 -0.18094427731775653 1103 22-23 -0.23744705944187672 1103 24-25 -0.3621666214731434 1103 26-27 -0.486760234056149 1103 28-29 -0.4262281321571777 1103 30-31 -0.421570978621034 1103 32-33 -0.19446493859619096 1103 34-35 -0.1109217050094955 1103 36-37 -0.13356682672467457 1103 38-39 -0.2984703169746581 1103 40-41 -0.2362246623177242 1103 42-43 -0.2311597216330199 1103 44-45 -0.25852856139383107 1103 46-47 -0.02238554032786766 1103 48-49 -0.12374684989320173 1103 50-51 -0.13366488819694666 1103 52-53 -0.06439132068952347 1103 54-55 0.053505282396585585 1103 56-57 0.02618142215465724 1103 58-59 0.06576005592317813 1103 60-61 0.12541692123816972 1103 62-63 0.21801641310744202 1103 64-65 0.005633213958233796 1103 66-67 0.019306000897941544 1103 68-69 0.17616936406896144 1103 70-71 0.13023988930252273 1103 72-73 0.005315663644260837 1103 74-75 0.048861644162691675 1103 76-77 0.2081759641725931 1103 78-79 0.03151585277021596 1103 80-81 -0.1747214558999879 1103 82-83 -0.1360338394231455 1103 84-85 -0.1647639687133413 1103 86-87 -0.0117867214951346 1103 88-89 -0.03175466549081207 1103 90-91 -0.1638653170764215 1103 92-93 -0.008234856318953376 1103 94-95 -0.5250640496644507 1103 96-97 -0.3213026725944239 1103 98-99 -0.09739458836409298 1103 100-101 0.1860677556908925 1104 1 0.03168358401680038 1104 2 -0.05183076524435748 1104 3 -0.050617524373453904 1104 4 -0.0474012324812918 1104 5 0.01981720540338472 1104 6 0.08736358396223665 1104 7 0.014962928457833868 1104 8 0.009890658827814036 1104 9 -0.023093644943607217 1104 10-11 0.002275197616604885 1104 12-13 -0.004695006139947111 1104 14-15 0.04854564262581107 1104 16-17 -0.08459713091584575 1104 18-19 -0.12165040507040459 1104 20-21 0.07958256969454425 1104 22-23 -0.19097860277647527 1104 24-25 -0.16498995623799573 1104 26-27 -0.10829632528314903 1104 28-29 -0.1055697564553526 1104 30-31 -0.18876905734429528 1104 32-33 -0.18589172603862636 1104 34-35 -0.038755875157669095 1104 36-37 -0.028228990357767714 1104 38-39 -0.047400632101251006 1104 40-41 -0.0026256724327282654 1104 42-43 -0.05770967621347012 1104 44-45 0.14004720415815086 1104 46-47 0.11948574521367306 1104 48-49 0.27180282563902125 1104 50-51 0.15276743073340526 1104 52-53 0.18453582793235057 1104 54-55 0.16748065094805042 1104 56-57 0.30111220434068997 1104 58-59 0.3710101207375729 1104 60-61 0.36004423352876813 1104 62-63 0.46200054891883013 1104 64-65 0.5254545439297971 1104 66-67 0.5823701087649198 1104 68-69 0.528753726681245 1104 70-71 0.6265606087323903 1104 72-73 0.6131873964545953 1104 74-75 0.6476468937921105 1104 76-77 0.49100964944973313 1104 78-79 0.4042676839005175 1104 80-81 0.44217956509987744 1104 82-83 0.42880608386827745 1104 84-85 0.5014904916054093 1104 86-87 0.4704866903543241 1104 88-89 0.1826036522243939 1104 90-91 0.5154803643059154 1104 92-93 0.5557010298768681 1104 94-95 0.4706569139820935 1104 96-97 0.4899975931940652 1104 98-99 0.564730650855445 1104 100-101 0.9154243219331804 1105 1 0.0531243161955004 1105 2 -0.08369750727705139 1105 3 -0.08788602487156894 1105 4 -0.08492178044195242 1105 5 5.004864430731004E-4 1105 6 0.0019944088930472503 1105 7 0.018556353561720584 1105 8 -0.07275563644680005 1105 9 -0.025062638818425853 1105 10-11 -0.156342986581123 1105 12-13 -0.06494484226397645 1105 14-15 -0.0636604947411783 1105 16-17 -0.21156147082565724 1105 18-19 -0.15092559373933057 1105 20-21 -0.07683115820476161 1105 22-23 0.07419981049786628 1105 24-25 -0.02306015721030974 1105 26-27 -0.08399258665433962 1105 28-29 -0.07761392455769567 1105 30-31 -0.07026556940906659 1105 32-33 -0.011981007703354862 1105 34-35 0.16681748769026683 1105 36-37 0.19841154641081715 1105 38-39 0.2695411544120674 1105 40-41 0.2919894926109663 1105 42-43 0.35133029824331174 1105 44-45 0.4576943625317398 1105 46-47 0.4834715023883902 1105 48-49 0.42552120265192883 1105 50-51 0.14786367472979123 1105 52-53 0.3306284717342294 1105 54-55 0.46180303928392163 1105 56-57 0.533899585269701 1105 58-59 0.6712173773126366 1105 60-61 0.7441092829849012 1105 62-63 0.7620526733269273 1105 64-65 0.5904684606954618 1105 66-67 0.45878968091605543 1105 68-69 0.4158022779724533 1105 70-71 0.39845810642233204 1105 72-73 0.4796427449040017 1105 74-75 0.628710164023321 1105 76-77 0.46583818609646244 1105 78-79 0.540036987137082 1105 80-81 0.4377250718517942 1105 82-83 0.46331018556277215 1105 84-85 0.5876038497824538 1105 86-87 0.4869912862152823 1105 88-89 0.5116862513594889 1105 90-91 0.6775702371652308 1105 92-93 0.828882683439101 1105 94-95 0.8327281709813761 1105 96-97 0.7204851820565921 1105 98-99 0.7735036851616535 1105 100-101 0.8525000072506828 1106 1 -0.18342742016259095 1106 2 -0.043064778844520646 1106 3 -0.1043007831348497 1106 4 -0.04744391893377298 1106 5 -0.006130724885757388 1106 6 -0.18134478866203807 1106 7 -0.3593636280777801 1106 8 -0.3081184967694739 1106 9 -0.4197475444803942 1106 10-11 -0.7224116713838455 1106 12-13 -0.32558924851363 1106 14-15 -0.3690282405090315 1106 16-17 -0.2349023390511107 1106 18-19 -0.45723667762469944 1106 20-21 -0.2836533997212527 1106 22-23 -0.2484403887000397 1106 24-25 -0.39085781709051304 1106 26-27 -0.5438247770972566 1106 28-29 -0.27770792086027996 1106 30-31 -0.3312913610157153 1106 32-33 -0.26588704346414005 1106 34-35 0.004226666083262387 1106 36-37 -0.2698760924003807 1106 38-39 -0.28565636017044227 1106 40-41 -0.21733335431258638 1106 42-43 -0.1678722857268582 1106 44-45 -0.08123056952345564 1106 46-47 0.08323330529485418 1106 48-49 0.059811036960240926 1106 50-51 -0.017670648686674895 1106 52-53 0.12271691938613571 1106 54-55 0.01474852395973869 1106 56-57 -6.887758954761125E-4 1106 58-59 0.1362113485551717 1106 60-61 -0.052642697561068985 1106 62-63 -0.06316440834944359 1106 64-65 -0.014885447196590462 1106 66-67 0.06957115807554004 1106 68-69 -0.06473736543224362 1106 70-71 0.22061904260525722 1106 72-73 0.03140610372771846 1106 74-75 0.16980232680736762 1106 76-77 0.26472617777348617 1106 78-79 0.3083972747961461 1106 80-81 -0.06479359029562559 1106 82-83 0.0688417076531671 1106 84-85 -0.12201085819196322 1106 86-87 0.1064408425172978 1106 88-89 0.44846276882179126 1106 90-91 0.5494990092309493 1106 92-93 0.6323211424397499 1106 94-95 0.7911149784231171 1106 96-97 0.941257943041883 1106 98-99 0.8721926308083319 1106 100-101 0.9333984228982821 1107 1 0.02968778719107945 1107 2 -0.08247782088949407 1107 3 0.017694295419005357 1107 4 0.0210851982047231 1107 5 -0.07729602720164763 1107 6 -0.07876645280843064 1107 7 -0.0011274369453175836 1107 8 -0.009962656646294477 1107 9 0.15235674594022441 1107 10-11 -0.03760860366823238 1107 12-13 0.01783847279725137 1107 14-15 0.03180802250972903 1107 16-17 -0.19395161093654423 1107 18-19 -0.0028259857085046747 1107 20-21 -0.0025253591756850824 1107 22-23 0.05225380318531592 1107 24-25 0.017320737399508346 1107 26-27 -0.05151692936858865 1107 28-29 -0.13379230856134683 1107 30-31 -0.09511192602496266 1107 32-33 0.069607663960781 1107 34-35 0.02551287773948019 1107 36-37 -0.10208115123866435 1107 38-39 0.1333763863750832 1107 40-41 0.1398755859601124 1107 42-43 0.12999802970082897 1107 44-45 0.19579059439644197 1107 46-47 0.3608021236586083 1107 48-49 0.2392811201399141 1107 50-51 0.427384722899518 1107 52-53 0.384787648691713 1107 54-55 0.3686087965746765 1107 56-57 0.38701919986736044 1107 58-59 0.4016615843124356 1107 60-61 0.5449530462600052 1107 62-63 0.7410374304933995 1107 64-65 0.7066609800759522 1107 66-67 0.5975259930667249 1107 68-69 0.5197928321274574 1107 70-71 0.6642031910398103 1107 72-73 0.646919718523538 1107 74-75 0.5798540721767615 1107 76-77 0.41912698000749415 1107 78-79 0.5327420567658727 1107 80-81 0.6591306143293458 1107 82-83 0.8176049656760398 1107 84-85 0.8612314475289544 1107 86-87 1.0885702931401333 1107 88-89 1.006037201972859 1107 90-91 1.0692815638315665 1107 92-93 1.0575094185937068 1107 94-95 1.1948261614205187 1107 96-97 1.286914033065635 1107 98-99 1.4062482299635626 1107 100-101 1.8952534933277079 1108 1 -0.0898888701755709 1108 2 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-0.7745528339897092 2316 74-75 -0.6546464914391059 2316 76-77 -0.6769028613316088 2316 78-79 -0.24857195605790494 2316 80-81 0.05875850664841309 2316 82-83 -0.12433653948134804 2316 84-85 0.14916946190418656 2316 86-87 0.22276238312474206 2316 88-89 -0.00550480256030994 2316 90-91 0.058109242825057805 2316 92-93 0.20334350947524982 2316 94-95 0.34851148195431847 2316 96-97 0.43447533386682125 2316 98-99 -0.24337360196981805 2316 100-101 0.15017452650225493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5692.0 3 1227.0 4 950.0 5 1007.0 6 1096.0 7 1275.0 8 1560.0 9 1978.0 10 2478.0 11 3244.0 12 4334.0 13 5914.0 14 6784.0 15 8890.0 16 11674.0 17 15761.0 18 13493.0 19 8713.0 20 8468.0 21 8713.0 22 9340.0 23 10379.0 24 11496.0 25 13020.0 26 14889.0 27 17746.0 28 20970.0 29 25720.0 30 31100.0 31 40125.0 32 51307.0 33 68112.0 34 94273.0 35 134444.0 36 198347.0 37 272984.0 38 213201.0 39 26065.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.673183753081815 19.063816489722623 14.858824987878116 22.404174769317443 2 15.26107981337718 19.892622942404184 40.634758133947784 24.211539110270856 3 18.77805243077681 25.03840538636002 30.11847557564083 26.065066607222338 4 12.480603555745894 16.394578675497854 37.342958467409574 33.78185930134668 5 13.266947872331064 37.58847565214585 34.25639386001707 14.88818261550601 6 31.506864386826187 36.24727782704401 17.5534954204414 14.692362365688409 7 28.170879836636974 33.183847950333885 20.764898660939572 17.88037355208957 8 26.778447048676686 34.19894433985304 19.786257056252737 19.23635155521754 9 26.622132786156705 16.37034451300449 20.430901594742178 36.57662110609663 10-11 24.69278278835512 26.87584940837538 27.17831763417627 21.253050169093232 12-13 25.803084866843186 24.246642658589 28.035616056563228 21.91465641800459 14-15 22.81936261676486 25.233786386123004 26.569734782984927 25.37711621412721 16-17 21.65508976150756 28.753167131001963 27.20133253289434 22.390410574596135 18-19 21.705711176234185 26.719381154790504 30.27038542885891 21.3045222401164 20-21 22.479976651037138 25.842769961383276 30.374514724366374 21.302738663213212 22-23 22.186392846313733 25.731337855045382 30.4382545785726 21.644014720068284 24-25 21.78150158768684 26.06611127263155 30.469985367837033 21.68240177184458 26-27 21.434003275031515 26.365763674136566 30.248758663323684 21.95147438750824 28-29 21.464747495453697 26.506514075662068 30.384669121920865 21.64406930696337 30-31 21.644469185730536 26.237070416585073 30.270902413962297 21.84755798372209 32-33 21.361730465847824 26.36055332309965 30.164484613167424 22.113231597885104 34-35 21.9073741561265 26.30002728560992 30.064047834901526 21.728550723362055 36-37 21.916629909792583 26.119489722924666 29.909499259546063 22.05438110773669 38-39 21.588644213212486 26.39325809739607 30.100607900506702 21.917489788884733 40-41 21.80345106734949 26.378073139397777 29.996326311528783 21.822149481723947 42-43 21.539673763192322 26.41720296376255 29.789934823036624 22.25318845000851 44-45 21.75222076878591 26.540566092052025 29.606607719983817 22.100605419178248 46-47 22.046539499823783 26.815204424006666 29.097070918258638 22.04118515791091 48-49 21.85189233040836 26.99900023398779 28.806867651664795 22.34223978393905 50-51 22.13521103412055 27.16547403228273 28.654619659597657 22.044695273999054 52-53 22.208221911647648 27.17284208750133 28.402313902858634 22.216622097992392 54-55 21.970950582836757 27.14401187678423 28.50215501433253 22.38288252604648 56-57 21.97407193262325 27.456114074990403 28.3386538948747 22.231160097511648 58-59 22.056253672706372 26.992297718422243 28.56611311367418 22.385335495197207 60-61 21.906839524945216 26.968354852639614 28.77994294591739 22.34486267649778 62-63 21.76862718070466 27.14268850805121 29.035357586440004 22.053326724804126 64-65 21.748304324572604 27.04561525442555 28.785004521678946 22.421075899322904 66-67 21.628396402684384 27.24431737992605 28.59417219620935 22.533114021180218 68-69 21.80116643491694 26.96672298802075 28.696285906696207 22.535824670366107 70-71 21.748561482333066 26.976495413166905 28.734427056449718 22.540516048050304 72-73 21.7526787586573 27.00153532793989 28.55537665392359 22.690409259479217 74-75 21.763422316856424 27.326872202767465 28.220593497222563 22.68911198315355 76-77 21.79038532755959 27.097595048564692 28.422353325095607 22.68966629878011 78-79 21.779417884372485 27.132227177983953 28.284762590904005 22.803592346739553 80-81 21.724695192592527 27.229889456904615 28.289749613583105 22.75566573691975 82-83 21.805097561199148 27.136439039143625 28.312159177351504 22.746304222305724 84-85 21.767961553365456 27.040039534719174 28.243128257975776 22.948870653939597 86-87 21.8261102931256 27.021287828571094 28.294949933420543 22.857651944882768 88-89 21.684345829427272 26.89691556566783 28.26372205005208 23.15501655485282 90-91 21.62894374725699 27.02528097406052 28.285498170959972 23.06027710772252 92-93 21.714497094026118 26.97329612924206 28.279068812537645 23.03313796419418 94-95 21.677767415124 26.647991893667438 28.372200617066706 23.302040074141857 96-97 21.710973528810033 26.841173593486587 28.098516619014763 23.34933625868862 98-99 21.60662016651615 27.015113465573865 27.95831632935541 23.41995003855457 100-101 21.87110741928176 26.495685765040804 28.240221763920548 23.39298505175689 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1976.0 1 1170.0 2 386.0 3 426.5 4 380.0 5 353.5 6 576.5 7 1122.5 8 1922.5 9 2412.0 10 2195.0 11 1967.0 12 2095.5 13 2205.5 14 2320.0 15 2367.0 16 2386.5 17 2525.0 18 2650.0 19 2809.0 20 3113.5 21 3483.0 22 3965.0 23 4708.5 24 5540.5 25 6725.0 26 8680.5 27 11083.5 28 12781.0 29 14864.0 30 17734.0 31 19870.5 32 22305.0 33 24163.5 34 26094.0 35 28855.0 36 32511.5 37 36189.0 38 37552.5 39 37936.5 40 38389.0 41 40213.5 42 42042.5 43 44056.0 44 46873.0 45 48656.0 46 49684.0 47 50647.0 48 52243.5 49 52920.0 50 51397.0 51 48969.0 52 47194.0 53 46685.5 54 45729.0 55 42393.0 56 37916.0 57 33607.5 58 30624.5 59 27500.0 60 22899.0 61 18658.0 62 14978.0 63 11224.0 64 7887.5 65 5681.5 66 4130.0 67 3103.5 68 2399.5 69 1785.0 70 1328.5 71 921.0 72 647.0 73 501.5 74 389.5 75 313.5 76 248.5 77 165.0 78 97.0 79 63.0 80 46.0 81 37.5 82 32.0 83 23.0 84 15.0 85 11.0 86 6.5 87 4.0 88 4.0 89 5.0 90 4.5 91 3.0 92 2.5 93 2.0 94 0.5 95 0.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2587845539935644 2 0.2317866743148465 3 0.221909401261657 4 0.22842108497820418 5 0.22842108497820418 6 0.25117539549332957 7 0.250955900536592 8 0.23903000788718545 9 0.2317866743148465 10-11 0.24327357705077796 12-13 0.2446637117767824 14-15 0.22944539477631268 16-17 0.245505109110943 18-19 0.23427428382453863 20-21 0.22798209506472905 22-23 0.22370194340834695 24-25 0.21931204427359605 26-27 0.2284942499637833 28-29 0.24170052986082555 30-31 0.24371256696425309 32-33 0.24166394736803595 34-35 0.25007792070964185 36-37 0.22560423303340568 38-39 0.22417751581461165 40-41 0.22110458642028602 42-43 0.2269577852666205 44-45 0.24426130435609691 46-47 0.24835854354853104 48-49 0.25278502517607154 50-51 0.25428490738044474 52-53 0.2713689315131836 54-55 0.2518338803635422 56-57 0.22238497366792168 58-59 0.20687399672513523 60-61 0.2052277845496037 62-63 0.21071515846804229 64-65 0.20409372727312633 66-67 0.20577652194144752 68-69 0.21031275104735678 70-71 0.20998350861225046 72-73 0.2118857982373092 74-75 0.2140807478046846 76-77 0.22893323987725842 78-79 0.22253130363908005 80-81 0.21671468728553514 82-83 0.21119073087430695 84-85 0.21287352554262814 86-87 0.2187633068817522 88-89 0.2176292496052749 90-91 0.22538473807666815 92-93 0.21550746502347864 94-95 0.21232478815078423 96-97 0.2242506808001908 98-99 0.22845766747099377 100-101 0.20493512460728697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1366774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.34101590644954 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32273376116864 41.47454889339379 2 12.89286202605231 14.012224408747356 3 4.399420951841319 7.172070117695293 4 2.01843292162423 4.387347820003348 5 1.0983424259028047 2.9842521618356344 6 0.6720581310970981 2.1912192955203693 7 0.45811517401253077 1.7426110770600585 8 0.3402035996479021 1.4789607375918432 9 0.24557834787570396 1.2010479217373904 >10 1.415174154956649 14.142945183667043 >50 0.09397836456772578 3.458012103808518 >100 0.04122040403597584 3.86103101244796 >500 0.0012084024967367601 0.3923167892862593 >1k 4.028008322455867E-4 0.5289746554445564 >5k 2.685338881637245E-4 0.9724378217606963 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7684 0.5621997491904295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5644 0.4129431786088995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3761 0.275173510763301 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2007 0.14684212605741695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1482 0.10843050862834676 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 3.658249278959067E-5 0.0 0.0 7.316498557918134E-5 0.0 16-17 1.0974747836877202E-4 0.0 0.0 7.316498557918134E-5 0.0 18-19 1.8291246394795335E-4 0.0 0.0 7.316498557918134E-5 0.0 20-21 2.1949495673754402E-4 0.0 0.0 7.316498557918134E-5 0.0 22-23 2.1949495673754402E-4 0.0 0.0 1.463299711583627E-4 0.0 24-25 2.1949495673754402E-4 0.0 0.0 2.926599423167254E-4 0.0 26-27 2.926599423167254E-4 0.0 0.0 6.950673630022227E-4 0.0 28-29 2.926599423167254E-4 0.0 0.0 0.001682794668321171 0.0 30-31 2.926599423167254E-4 0.0 0.0 0.0038777442356966113 0.0 32-33 2.926599423167254E-4 0.0 0.0 0.007426246036286907 0.0 34-35 2.926599423167254E-4 0.0 0.0 0.011194242793614745 0.0 36-37 2.926599423167254E-4 0.0 0.0 0.018766818801060015 0.0 38-39 2.926599423167254E-4 0.0 0.0 0.030253721536991483 0.0 40-41 2.926599423167254E-4 0.0 0.0 0.049276617787578636 0.0 42-43 2.926599423167254E-4 0.0 0.0 0.07177485085317689 0.0 44-45 3.658249278959067E-4 0.0 0.0 0.09555347116641083 0.0 46-47 4.7557240626467875E-4 0.0 0.0 0.13908663758602374 0.0 48-49 5.121548990542694E-4 0.0 0.0 0.1928994844795116 0.0 50-51 5.121548990542694E-4 0.0 0.0 0.23643265089912452 0.0 52-53 5.121548990542694E-4 0.0 0.0 0.29079423518445624 0.0 54-55 5.121548990542694E-4 0.0 0.0 0.3624593385592644 0.0 56-57 5.853198846334507E-4 0.0 0.0 0.4675242578509688 7.316498557918134E-5 58-59 5.853198846334507E-4 0.0 0.0 0.591465743422102 7.316498557918134E-5 60-61 5.853198846334507E-4 0.0 0.0 0.7315766908062342 7.316498557918134E-5 62-63 5.853198846334507E-4 0.0 0.0 0.8894301471933179 7.316498557918134E-5 64-65 5.853198846334507E-4 0.0 0.0 1.068538031891154 7.316498557918134E-5 66-67 5.853198846334507E-4 0.0 0.0 1.267912617594423 7.316498557918134E-5 68-69 5.853198846334507E-4 0.0 0.0 1.5122105044433096 7.316498557918134E-5 70-71 5.853198846334507E-4 0.0 0.0 1.7685074489271817 7.316498557918134E-5 72-73 5.853198846334507E-4 0.0 0.0 2.0468636365631774 7.316498557918134E-5 74-75 5.853198846334507E-4 0.0 0.0 2.3367433094278938 7.316498557918134E-5 76-77 5.853198846334507E-4 0.0 0.0 2.691154499573448 7.316498557918134E-5 78-79 5.853198846334507E-4 0.0 0.0 3.095317879912846 7.316498557918134E-5 80-81 6.584848702126321E-4 0.0 0.0 3.565329747273507 7.316498557918134E-5 82-83 6.950673630022227E-4 0.0 0.0 4.048584477024 7.316498557918134E-5 84-85 7.316498557918134E-4 0.0 0.0 4.590115117788311 7.316498557918134E-5 86-87 7.316498557918134E-4 3.658249278959067E-5 0.0 5.135011347889263 7.316498557918134E-5 88-89 7.316498557918134E-4 7.316498557918134E-5 0.0 5.817933323285342 7.316498557918134E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3465 0.0 56.920113 1 GTATCAA 6375 0.0 42.64192 1 ATCAACG 8145 0.0 33.368004 3 TCAACGC 8180 0.0 33.16836 4 TATCAAC 8325 0.0 32.703606 2 CAACGCA 8340 0.0 32.53323 5 AACGCAG 8535 0.0 31.837437 6 TATGCCG 70 0.008376708 27.15308 5 ACGCAGA 9825 0.0 27.07703 7 CGCAGAG 9890 0.0 26.61087 8 TAAACCG 90 8.928754E-4 26.398827 5 GTACATG 6820 0.0 25.922653 1 TACATGG 6965 0.0 25.17274 2 ACATGGG 6940 0.0 24.236454 3 TGGTATC 1360 0.0 24.106571 2 GCAGAGT 10690 0.0 24.041698 9 GAGTACT 6150 0.0 23.828777 12-13 GTACTTT 6565 0.0 22.1749 14-15 CAGAGTA 10400 0.0 21.261803 10-11 AGTACTT 6395 0.0 21.058828 12-13 >>END_MODULE