FastQCFastQC Report
Thu 26 May 2016
SRR937148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366774
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.6071230503360467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67050.4905712283084109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46460.3399245230008765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16850.12328300070092056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.054.0916561
GTATCAA73450.042.242391
ATCAACG93300.033.040543
TATCAAC93950.032.8119472
TCAACGC93950.032.8119474
CAACGCA96250.032.0772175
AACGCAG98650.031.2956856
ACGCAGA115100.026.581177
CGCAGAG115650.026.331528
GCAGAGT123200.024.2551219
GAGTACT72400.023.29449712-13
GTACTTT73350.022.79348414-15
GTACATG68750.022.4614471
TGGTATC18050.022.367932
TACATGG67850.021.9118252
AGTACTT72850.021.7159212-13
TAAGGTG6150.021.625545
GTGTAAG5150.021.2201141
CAGAGTA119450.020.84052510-11
AGAGTAC111600.020.75266810-11
Produced by FastQC (version 0.11.5)