##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937148_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1366774 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07722783722839 31.0 31.0 34.0 31.0 34.0 2 32.30324033088133 34.0 31.0 34.0 31.0 34.0 3 32.34675301110498 34.0 31.0 34.0 30.0 34.0 4 36.035322591737916 37.0 35.0 37.0 35.0 37.0 5 35.943488828438355 37.0 35.0 37.0 35.0 37.0 6 35.99312688125469 37.0 35.0 37.0 35.0 37.0 7 35.923710869536585 37.0 35.0 37.0 35.0 37.0 8 35.895248226846576 37.0 35.0 37.0 35.0 37.0 9 37.574250753965174 39.0 37.0 39.0 35.0 39.0 10-11 37.48708052684643 39.0 37.0 39.0 35.0 39.0 12-13 37.379723714381456 39.0 37.0 39.0 34.0 39.0 14-15 38.5263800013755 40.0 38.0 41.0 34.0 41.0 16-17 38.48253259134283 40.0 38.0 41.0 34.0 41.0 18-19 38.49448811581139 40.0 38.0 41.0 34.0 41.0 20-21 38.3940578325312 40.0 38.0 41.0 34.0 41.0 22-23 38.229165172881544 40.0 38.0 41.0 34.0 41.0 24-25 38.15666635449606 40.0 37.5 41.0 33.5 41.0 26-27 38.093177804084654 40.0 37.5 41.0 33.0 41.0 28-29 37.94572657952229 40.0 37.0 41.0 33.0 41.0 30-31 38.01547219950043 40.0 37.0 41.0 33.0 41.0 32-33 37.942268802303815 40.0 37.0 41.0 33.0 41.0 34-35 37.7992349137458 40.0 37.0 41.0 33.0 41.0 36-37 37.602602917526966 40.0 37.0 41.0 33.0 41.0 38-39 37.453572426750874 40.0 36.0 41.0 33.0 41.0 40-41 37.254439651324944 40.0 36.0 41.0 32.0 41.0 42-43 37.010594655736796 39.0 36.0 41.0 31.5 41.0 44-45 36.67288630014911 39.0 36.0 41.0 31.0 41.0 46-47 36.261277650877176 39.0 35.0 41.0 30.0 41.0 48-49 35.88186086361023 39.0 35.0 40.0 29.0 41.0 50-51 35.605672554496934 39.0 35.0 40.0 28.0 41.0 52-53 35.339140194355465 38.5 35.0 40.0 27.0 41.0 54-55 35.08982831104484 38.0 34.5 40.0 27.0 41.0 56-57 34.7879411665718 38.0 34.0 40.0 26.0 41.0 58-59 34.48302682082041 38.0 34.0 40.0 26.0 41.0 60-61 34.12249647710594 37.5 33.5 40.0 25.0 41.0 62-63 33.72905359627853 37.0 33.0 40.0 23.5 41.0 64-65 33.30574330503799 36.0 32.5 39.0 22.5 40.5 66-67 32.861413445090406 36.0 32.0 39.0 22.0 40.0 68-69 32.39097246508933 35.0 31.5 38.5 20.5 40.0 70-71 31.961085007470146 35.0 31.0 38.0 19.5 40.0 72-73 31.475836897687547 35.0 31.0 37.0 18.0 39.0 74-75 30.98334691763232 34.5 30.0 36.5 16.0 39.0 76-77 29.788057498898866 33.5 29.0 35.0 13.5 37.5 78-79 30.400901685282278 34.0 30.0 36.0 13.0 38.0 80-81 30.192111131759894 34.0 30.0 35.0 9.0 37.0 82-83 29.83685671515554 34.0 30.0 35.0 7.0 37.0 84-85 29.45570811267993 34.0 29.5 35.0 3.5 36.0 86-87 29.016633693646497 34.0 29.0 35.0 2.0 36.0 88-89 28.520456929967942 33.5 29.0 35.0 2.0 35.5 90-91 27.968437356870997 33.0 27.0 35.0 2.0 35.0 92-93 27.529550971850504 33.0 26.5 35.0 2.0 35.0 94-95 27.02066069445278 33.0 25.5 35.0 2.0 35.0 96-97 26.46764022435311 32.5 25.0 34.0 2.0 35.0 98-99 25.594892059696775 32.0 23.5 34.0 2.0 35.0 100-101 23.062453997515316 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0015555933792654741 1101 2 -5.334052401053668E-4 1101 3 -0.023713064800730876 1101 4 -0.03608534739585423 1101 5 -0.009877621334773323 1101 6 -0.1377816622213146 1101 7 -0.06653967345695833 1101 8 -0.03680461125346568 1101 9 0.03272692016724221 1101 10-11 -0.06188841472955886 1101 12-13 -0.076705961907642 1101 14-15 -0.1593792242532004 1101 16-17 -0.1316008494141201 1101 18-19 -0.17879801670218143 1101 20-21 -0.07992719345672583 1101 22-23 -0.09442888549392592 1101 24-25 -0.13873465854882738 1101 26-27 -0.16377256453598932 1101 28-29 -0.1504023215871797 1101 30-31 -0.14484161839516219 1101 32-33 -0.05283363697846255 1101 34-35 -0.033472359991563394 1101 36-37 -0.03625320304545454 1101 38-39 -0.12616455977004648 1101 40-41 -0.059160962484504864 1101 42-43 -0.025538120726864122 1101 44-45 0.024593719474772513 1101 46-47 -0.16922188028446072 1101 48-49 -0.13861879077661854 1101 50-51 -0.01745769087315807 1101 52-53 0.10970689563557556 1101 54-55 -0.008605513234037687 1101 56-57 -0.10317512936613582 1101 58-59 0.028920570843347093 1101 60-61 0.06121458167651639 1101 62-63 -0.09159267740742649 1101 64-65 9.966842141508891E-4 1101 66-67 0.278340942971667 1101 68-69 0.1142858123269761 1101 70-71 0.08707211282008487 1101 72-73 0.042795124992220934 1101 74-75 0.12808333324129606 1101 76-77 0.15545386284264495 1101 78-79 0.1553236997189238 1101 80-81 0.24241586563829998 1101 82-83 0.16355498100813648 1101 84-85 0.1981016338946482 1101 86-87 0.15914624983406966 1101 88-89 0.22446267480657411 1101 90-91 0.19068178830343996 1101 92-93 0.18435756189140307 1101 94-95 0.2745605256644872 1101 96-97 0.2299224937968063 1101 98-99 0.5208904451323555 1101 100-101 0.874495953316714 1102 1 -0.08979744959270519 1102 2 -0.1292463485856743 1102 3 -0.06018667836027447 1102 4 -0.015923131031897242 1102 5 -0.04220768230742067 1102 6 -0.04883841368901898 1102 7 -0.111416838633005 1102 8 -0.16710494603698578 1102 9 -0.20300515597579505 1102 10-11 -0.15056965876571127 1102 12-13 -0.24309984200015577 1102 14-15 -0.185513768413891 1102 16-17 -0.16034654332170106 1102 18-19 -0.2509386096086601 1102 20-21 -0.054187677679855994 1102 22-23 -0.08997076578050667 1102 24-25 -0.17512659786729046 1102 26-27 -0.24330022865268575 1102 28-29 -0.322931877958581 1102 30-31 -0.14900821767384542 1102 32-33 -0.12331405942028084 1102 34-35 -0.16747552992250547 1102 36-37 -0.23865410227076467 1102 38-39 -0.01949961823586932 1102 40-41 -0.04402535089808168 1102 42-43 -0.03135235630448108 1102 44-45 -0.13130946824659873 1102 46-47 0.059269491357603954 1102 48-49 0.10151116021884121 1102 50-51 -0.10981751038116982 1102 52-53 -0.10810860144518841 1102 54-55 0.030101607789397633 1102 56-57 0.10348859918427422 1102 58-59 0.1590454273069284 1102 60-61 0.048963849842969864 1102 62-63 -0.012917336558984971 1102 64-65 0.11768329772517205 1102 66-67 0.021592709884707517 1102 68-69 0.1515915327584807 1102 70-71 0.23349178903428935 1102 72-73 0.20539721779356768 1102 74-75 0.11077445905197081 1102 76-77 0.13870943043818684 1102 78-79 -0.03298151934594884 1102 80-81 0.21205447733793648 1102 82-83 0.2311266683494395 1102 84-85 0.32122595664537457 1102 86-87 0.2872793869335801 1102 88-89 0.30010717352081073 1102 90-91 0.13213914788035197 1102 92-93 0.18452131470148103 1102 94-95 0.10337535964744049 1102 96-97 0.0679086981279653 1102 98-99 0.448301452555814 1102 100-101 1.0386793726371195 1103 1 -0.022834837132108987 1103 2 -0.01864579283466128 1103 3 -0.08867214913308175 1103 4 -0.01719511155906872 1103 5 -0.032712525383153945 1103 6 -0.024468300743755833 1103 7 -0.08467042337067454 1103 8 -0.125248421706722 1103 9 -0.09768567094301517 1103 10-11 -0.13020253280267013 1103 12-13 -0.19651584389574595 1103 14-15 -0.24126819683043976 1103 16-17 -0.3360071259268409 1103 18-19 -0.18881925238425623 1103 20-21 -0.26340926724265046 1103 22-23 -0.25615611664321136 1103 24-25 -0.10586092159585547 1103 26-27 -0.2474408968463564 1103 28-29 -0.30185660026258176 1103 30-31 -0.2640118795991597 1103 32-33 -0.18624047626225604 1103 34-35 -0.123777990592707 1103 36-37 -0.15816965109284098 1103 38-39 -0.08994105974200295 1103 40-41 -0.1749445659536164 1103 42-43 -0.179997443939385 1103 44-45 -0.17685843393220324 1103 46-47 0.08418458495210501 1103 48-49 0.0422592341179282 1103 50-51 0.02944766327307491 1103 52-53 0.1927821080348977 1103 54-55 0.03190779859723847 1103 56-57 0.12172732081332072 1103 58-59 0.014700229814273769 1103 60-61 -0.006054244376215934 1103 62-63 -0.054623973260092384 1103 64-65 -0.23274033677159878 1103 66-67 -0.08107654645724693 1103 68-69 -0.09196060659573391 1103 70-71 0.006438238761791837 1103 72-73 0.13312481363189832 1103 74-75 0.28496452733610766 1103 76-77 0.33262686479279324 1103 78-79 0.3627817766740442 1103 80-81 0.31849282415816305 1103 82-83 0.21654735894546917 1103 84-85 0.21386440973323673 1103 86-87 0.2410357324757264 1103 88-89 0.27752153114012756 1103 90-91 0.3867711243865841 1103 92-93 0.5651753991842519 1103 94-95 0.5572814133125981 1103 96-97 0.7345157089198793 1103 98-99 1.0289966439452662 1103 100-101 1.4659540744774162 1104 1 0.028496381239953905 1104 2 3.955874447498786E-4 1104 3 0.004057753952082521 1104 4 -0.06929104081935122 1104 5 -0.05284041259225347 1104 6 -0.0398406915933478 1104 7 -0.07942075436937301 1104 8 -0.08215229256082779 1104 9 -0.025401097164447606 1104 10-11 -0.032179817089478036 1104 12-13 0.026044908465777894 1104 14-15 -0.10083173373703858 1104 16-17 -0.11391165185160901 1104 18-19 -0.0735044523391295 1104 20-21 -0.11473098610056809 1104 22-23 -0.3012915737776467 1104 24-25 -0.1441329797645423 1104 26-27 -0.2394226142397713 1104 28-29 -0.22408071359178905 1104 30-31 -0.039096039357232826 1104 32-33 0.009656723162223102 1104 34-35 0.03223737882954225 1104 36-37 -0.11969760836085896 1104 38-39 -0.15493703234380973 1104 40-41 -0.09803333297605832 1104 42-43 -0.0723207480287229 1104 44-45 0.21050325413145998 1104 46-47 0.1115059792432973 1104 48-49 0.3658483088850417 1104 50-51 0.33932498100609365 1104 52-53 0.2009888966251836 1104 54-55 0.1513595380968198 1104 56-57 0.16804883186937047 1104 58-59 0.11801961733310407 1104 60-61 0.2346125819350604 1104 62-63 0.2845887347149514 1104 64-65 0.2800479883255207 1104 66-67 0.31263922807969635 1104 68-69 0.44636458948017577 1104 70-71 0.4440478047725307 1104 72-73 0.647802948323136 1104 74-75 0.635585261213155 1104 76-77 0.5177663592207296 1104 78-79 0.31552278231636066 1104 80-81 0.44555990853957894 1104 82-83 0.4139251961831718 1104 84-85 0.3478642593242647 1104 86-87 0.30184291410841624 1104 88-89 0.3259778600924683 1104 90-91 0.2973050960344956 1104 92-93 0.5003287470703164 1104 94-95 0.6390791567821772 1104 96-97 0.45397912490557957 1104 98-99 0.6456705671852312 1104 100-101 1.2585644420096607 1105 1 0.01516020475708757 1105 2 -0.03919256203722199 1105 3 -0.02878581302721983 1105 4 -0.0682068080015128 1105 5 -0.09444449048101689 1105 6 -0.1320726880822889 1105 7 -0.061997888903370324 1105 8 -0.09020901113753865 1105 9 -0.13186057495008185 1105 10-11 -0.22791565690733506 1105 12-13 -0.15396030397503324 1105 14-15 -0.1247164462799546 1105 16-17 -0.08567094088370908 1105 18-19 -0.1184766470136367 1105 20-21 -0.07137972512990132 1105 22-23 -0.09846015787789497 1105 24-25 -0.1731841011726658 1105 26-27 -0.20624655671788616 1105 28-29 -0.2247076731931088 1105 30-31 -0.1675759359207447 1105 32-33 -0.20626333469360958 1105 34-35 -0.09744679464319717 1105 36-37 -0.13158919410494718 1105 38-39 -0.0948941143246742 1105 40-41 -0.11748758365384049 1105 42-43 -0.04245064160241441 1105 44-45 0.053681669662275056 1105 46-47 -0.001672018840963574 1105 48-49 0.11090466981159608 1105 50-51 0.06220488840065741 1105 52-53 0.03764578150357778 1105 54-55 0.12278802893725071 1105 56-57 -0.0634794399808527 1105 58-59 0.06604402328972725 1105 60-61 0.06984676381314614 1105 62-63 0.054113330778989166 1105 64-65 0.26257480362572494 1105 66-67 0.37214510674265 1105 68-69 0.3542028819657972 1105 70-71 0.43124832917567346 1105 72-73 0.43046752098760876 1105 74-75 0.49742207119938087 1105 76-77 0.6410791699786671 1105 78-79 0.3699423567239499 1105 80-81 0.33563449608545426 1105 82-83 0.12458294528876479 1105 84-85 0.1824210495438301 1105 86-87 0.2914910292243782 1105 88-89 0.4746781910034876 1105 90-91 0.7245715769938563 1105 92-93 0.9416004472398889 1105 94-95 1.0550781910139229 1105 96-97 0.7898354149790379 1105 98-99 1.0569951025502924 1105 100-101 1.4029922638073664 1106 1 -0.17753896572975947 1106 2 -0.055819107301843474 1106 3 -0.12326222240221085 1106 4 -0.02832756878091658 1106 5 -0.1107149635760507 1106 6 -0.03177424560270481 1106 7 -0.019005971404112643 1106 8 -0.13278747990908357 1106 9 -0.1810677237317151 1106 10-11 -0.20314332793378043 1106 12-13 -0.16681054356434544 1106 14-15 -0.07568100528625621 1106 16-17 -0.11736136397676233 1106 18-19 -0.10585211667874006 1106 20-21 -0.030163949614006924 1106 22-23 -0.09695191379464063 1106 24-25 -0.047041738119119714 1106 26-27 -0.03058147381297971 1106 28-29 -0.08673097313834432 1106 30-31 -0.12000950356981832 1106 32-33 -0.1362930439378971 1106 34-35 -0.07792484262518684 1106 36-37 0.043342650137951466 1106 38-39 -0.18451292303545586 1106 40-41 -0.005400367910390003 1106 42-43 -0.10695834651478009 1106 44-45 0.1300857288850139 1106 46-47 0.20365801027763553 1106 48-49 0.1277109387068336 1106 50-51 0.2218737244074802 1106 52-53 0.25375543148125956 1106 54-55 0.3198566774577358 1106 56-57 0.3319867993992105 1106 58-59 0.31556876819607993 1106 60-61 0.43241345351481897 1106 62-63 0.5380373473405626 1106 64-65 0.6635116446918232 1106 66-67 0.5746894195866403 1106 68-69 0.6502152955529041 1106 70-71 0.5624900338332139 1106 72-73 0.4365325606913366 1106 74-75 0.534758178858052 1106 76-77 0.6773875622284464 1106 78-79 0.6963495800951129 1106 80-81 0.6683552502834331 1106 82-83 0.262093882695158 1106 84-85 0.33879157723097464 1106 86-87 0.5458552336773081 1106 88-89 0.4752540660170901 1106 90-91 0.5435641458858029 1106 92-93 0.8260558719825148 1106 94-95 0.7082014586426588 1106 96-97 0.649905802515498 1106 98-99 0.8290873978066848 1106 100-101 1.3267375523903588 1107 1 -0.052783283416125926 1107 2 -0.028107792442014556 1107 3 -0.036514821899857 1107 4 -0.09189376691060147 1107 5 -0.09982780591324314 1107 6 -0.03379471830825054 1107 7 -0.05554143495371733 1107 8 -0.008874015691652914 1107 9 0.014331128214422506 1107 10-11 -0.021445939447922058 1107 12-13 -0.016559154223514838 1107 14-15 -0.03619086625646872 1107 16-17 -0.018887777935454153 1107 18-19 -0.06130813736837837 1107 20-21 0.0700913743315823 1107 22-23 0.048896971861488225 1107 24-25 -0.04224311544346193 1107 26-27 -0.17772027178060057 1107 28-29 -0.21268564649172106 1107 30-31 -0.30585683665218966 1107 32-33 -0.23628570643175806 1107 34-35 -0.05624782901145409 1107 36-37 -0.15840420802700095 1107 38-39 -0.11763314966868421 1107 40-41 -0.1834146473353897 1107 42-43 -0.01509117451725217 1107 44-45 0.20328107456661826 1107 46-47 0.1504850174045842 1107 48-49 0.335252299909925 1107 50-51 0.3116066391839425 1107 52-53 0.39202913960165375 1107 54-55 0.5840919710749546 1107 56-57 0.6044416950045743 1107 58-59 0.3673274301752443 1107 60-61 0.525285544831668 1107 62-63 0.5816620115699038 1107 64-65 0.7890563747672985 1107 66-67 0.7001623697107462 1107 68-69 0.782458424350601 1107 70-71 0.7279901433640958 1107 72-73 0.9307694281504517 1107 74-75 1.029954770852303 1107 76-77 1.0185762807595822 1107 78-79 0.8943314468842836 1107 80-81 0.8432053631108829 1107 82-83 0.7801101187884569 1107 84-85 0.8271472740069363 1107 86-87 0.9474263107168674 1107 88-89 1.0284733170810298 1107 90-91 1.112688770769097 1107 92-93 1.296216148638397 1107 94-95 1.3568003139917018 1107 96-97 1.4231002974717626 1107 98-99 1.540072069862692 1107 100-101 2.0575733687783035 1108 1 -0.09835622240916209 1108 2 -0.005692629577410457 1108 3 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-0.6938205772988404 2316 94-95 -0.664589817276255 2316 96-97 -0.6413246325307824 2316 98-99 -0.8120090665808313 2316 100-101 -0.8534874411316373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 15.0 5 34.0 6 56.0 7 154.0 8 382.0 9 798.0 10 1304.0 11 2228.0 12 3217.0 13 4584.0 14 6039.0 15 8488.0 16 11796.0 17 16261.0 18 14389.0 19 8951.0 20 7929.0 21 7932.0 22 8315.0 23 9387.0 24 10768.0 25 12560.0 26 15145.0 27 18646.0 28 23181.0 29 28242.0 30 35661.0 31 45065.0 32 57131.0 33 74107.0 34 100557.0 35 141305.0 36 208488.0 37 284125.0 38 186616.0 39 12911.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.682543807951504 20.17014360442344 15.416445506825147 21.73086708079991 2 16.041203593278773 21.06119958383756 38.993718054338174 23.903878768545496 3 19.285046393917355 25.26818625464049 30.333325041301634 25.11344231014052 4 13.171160703964224 17.369952896382284 36.632610804712414 32.82627559494108 5 13.376018472992907 38.15322750579476 33.87125096579335 14.599503055418978 6 31.805111891212444 36.85693465049818 16.781852742296824 14.556100715992548 7 28.193688203024053 33.69262218918417 20.370229460027772 17.743460147764004 8 27.25037204395167 34.874383036259104 19.069795006343405 18.80544991344582 9 26.79982206275507 16.44090390949784 20.08539817116802 36.673875856579066 10-11 25.047392723433553 27.341961455137692 26.59432340896902 21.016322412459733 12-13 26.063230417565435 24.60707815413073 27.673588042297997 21.65610338600584 14-15 22.94914887172276 25.731503525820653 26.263559300952462 25.05578830150413 16-17 21.679443711981644 29.134553335079538 27.07225188655915 22.113751066379663 18-19 21.778838344890964 26.981527304440966 30.231333051404256 21.008301299263817 20-21 22.4868559103407 26.052588065034893 30.59902368643243 20.861532338191978 22-23 22.267881705639613 25.92880242324914 30.48556440047997 21.317751470631276 24-25 21.992035953444898 26.277512451825203 30.54799005683117 21.182461537898728 26-27 21.576963247358282 26.678873038490313 30.298348847375845 21.44581486677556 28-29 21.616605718449968 26.776424673711197 30.430025154858225 21.17694445298061 30-31 21.74915722989744 26.48209559381682 30.339248119200523 21.429499057085213 32-33 21.61955817128508 26.542061818559613 30.111342475054396 21.7270375351009 34-35 22.039488606016796 26.534160000117062 30.128755741622243 21.2975956522439 36-37 22.202032743537973 26.305858285955942 29.89376074382478 21.598348226681306 38-39 21.93338237838759 26.557604221045157 30.08815305861518 21.420860341952068 40-41 22.076993737440766 26.584763313014193 29.95821911970052 21.38002382984452 42-43 21.82559181431916 26.67674778269719 29.73280408083967 21.764856322143977 44-45 22.063307582869555 26.735046787264732 29.613106506830146 21.588539123035563 46-47 22.351427522033635 27.115455810543658 28.92270411933502 21.610412548087687 48-49 22.25693494315812 27.356827097969376 28.56968306391547 21.81655489495703 50-51 22.41709309659095 27.483219610557413 28.43564481033441 21.664042482517225 52-53 22.52351980778088 27.5014111701756 28.183199337710306 21.79186968433321 54-55 22.29816341253199 27.537032457450906 28.240733288751468 21.924070841265635 56-57 22.347293700348413 27.88222485941348 28.058589057152094 21.711892383086013 58-59 22.365036209351363 27.423516982324802 28.171811872335876 22.039634935987955 60-61 22.186294149581425 27.37658164407576 28.552855117232255 21.88426908911056 62-63 22.011137174104864 27.530959763647832 28.75691226201259 21.700990800234713 64-65 21.975944816041277 27.506339746000435 28.508517135971275 22.009198301987016 66-67 21.95626343492048 27.62274523805691 28.261621892134325 22.159369434888283 68-69 22.13460308726973 27.394763142992183 28.317300446160083 22.153333323578 70-71 22.057779852411592 27.418651510783786 28.273913609711627 22.24965502709299 72-73 22.178867903545136 27.28519857708736 28.262829114396382 22.27310440497112 74-75 22.08016833799882 27.67853353956104 27.788244435437022 22.45305368700312 76-77 22.080424415448345 27.426004591834495 28.12557160144984 22.367999391267322 78-79 22.064437866099297 27.525984544628447 27.94123242028309 22.468345168989167 80-81 21.954726970223316 27.672314515786812 27.973022606517244 22.39993590747263 82-83 22.021891767412136 27.559584845871964 27.85275790833787 22.56576547837803 84-85 21.93749567867881 27.508003361210502 27.88297514687005 22.67152581324064 86-87 22.025221433828857 27.528874561558826 27.755429939404756 22.69047406520756 88-89 21.810884608574643 27.409103480165705 27.748369518296368 23.031642392963285 90-91 21.678283301497498 27.559174958557314 27.765592638623083 22.996949101322105 92-93 21.739343573804412 27.46189090196044 27.801459537847162 22.997305986387985 94-95 21.535491602854606 27.37526467433533 27.816632449841745 23.27261127296832 96-97 21.738707350300782 27.410127789963813 27.541349191600073 23.309815668135332 98-99 21.44672943009901 27.56779200635073 27.528209705711816 23.457268857838447 100-101 21.42526812912932 27.34218303979441 27.65196667054491 23.580582160531364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 357.0 1 259.5 2 240.0 3 342.5 4 316.5 5 317.5 6 583.0 7 1214.5 8 1975.0 9 2475.0 10 2364.5 11 2153.5 12 2240.0 13 2318.0 14 2472.0 15 2581.5 16 2624.5 17 2736.0 18 2881.0 19 2995.0 20 3169.0 21 3517.0 22 4076.5 23 4847.5 24 5699.0 25 6845.5 26 8758.5 27 11114.0 28 12758.0 29 15085.5 30 18068.5 31 19853.0 32 22089.5 33 24188.0 34 26301.0 35 28928.5 36 32707.5 37 36416.0 38 37931.5 39 38168.5 40 38403.0 41 40500.0 42 42481.5 43 44324.0 44 46706.0 45 48529.0 46 49343.5 47 50206.0 48 51974.5 49 52524.5 50 51324.5 51 48750.5 52 46696.0 53 46477.0 54 45974.5 55 42787.5 56 37779.0 57 33488.5 58 30635.5 59 27273.0 60 22688.0 61 18753.5 62 15178.0 63 11279.5 64 7800.5 65 5456.5 66 3960.0 67 3071.0 68 2376.5 69 1722.0 70 1260.5 71 835.0 72 608.0 73 488.0 74 348.0 75 284.0 76 216.0 77 141.0 78 100.5 79 69.0 80 53.5 81 43.5 82 27.5 83 15.0 84 11.5 85 8.0 86 3.5 87 4.0 88 2.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06358037246830858 2 0.0 3 0.0 4 0.0 5 0.0016096296827419895 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1949495673754404E-4 12-13 0.006584848702126321 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010243097981085388 24-25 4.755724062646787E-4 26-27 1.0974747836877202E-4 28-29 0.0029265994231672536 30-31 1.0974747836877202E-4 32-33 0.0 34-35 0.0 36-37 3.658249278959067E-5 38-39 2.560774495271347E-4 40-41 3.658249278959067E-5 42-43 0.01408425972399241 44-45 1.8291246394795335E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.658249278959067E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.2924243510631604E-4 84-85 3.2924243510631604E-4 86-87 0.0 88-89 0.0 90-91 0.009328535661345621 92-93 0.0029265994231672536 94-95 0.0 96-97 0.0 98-99 1.0974747836877202E-4 100-101 0.012803872476356736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1366774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.714544635100246 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25665550244065 39.6712032557177 2 13.392772347900861 14.119877914423126 3 4.530680417577037 7.16498265299218 4 2.1613113198785348 4.557301681683377 5 1.189612745809077 3.1354947093718373 6 0.7141937627288654 2.2589039390088583 7 0.49593892005481394 1.8300236036259032 8 0.3475207259548271 1.4655517455974538 9 0.2596416440396133 1.2318201930471304 >10 1.4987378289526605 14.472971744713304 >50 0.10768575503432806 3.91057793008391 >100 0.04275063690898813 3.922461262238998 >500 0.001804394742012872 0.6052421782822263 >1k 4.1639878661835504E-4 0.5558540653300378 >5k 2.7759919107890336E-4 1.097733123883975 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8298 0.6071230503360467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6705 0.4905712283084109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4646 0.3399245230008765 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1685 0.12328300070092056 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.316498557918134E-5 0.0 16-17 0.0 0.0 0.0 7.316498557918134E-5 0.0 18-19 0.0 0.0 0.0 7.316498557918134E-5 0.0 20-21 0.0 0.0 0.0 7.316498557918134E-5 0.0 22-23 0.0 0.0 0.0 1.463299711583627E-4 0.0 24-25 0.0 0.0 0.0 2.926599423167254E-4 0.0 26-27 0.0 0.0 0.0 6.950673630022227E-4 0.0 28-29 0.0 0.0 0.0 0.001682794668321171 0.0 30-31 0.0 0.0 0.0 0.0038777442356966113 0.0 32-33 0.0 0.0 0.0 0.007279916065128544 0.0 34-35 0.0 0.0 0.0 0.011084495315245973 0.0 36-37 0.0 0.0 0.0 0.018693653815480833 0.0 38-39 1.463299711583627E-4 0.0 0.0 0.030326886522570665 0.0 40-41 1.463299711583627E-4 0.0 0.0 0.049459530251526586 0.0 42-43 1.463299711583627E-4 0.0 0.0 0.07192118082433525 0.0 44-45 1.463299711583627E-4 0.0 0.0 0.09610220855825469 0.0 46-47 2.1949495673754402E-4 0.0 0.0 0.14011094738413227 0.0 48-49 2.1949495673754402E-4 0.0 0.0 0.19374088181367222 0.0 50-51 2.1949495673754402E-4 0.0 0.0 0.23753012568281223 0.0 52-53 2.1949495673754402E-4 0.0 0.0 0.29185512747535436 0.0 54-55 2.1949495673754402E-4 0.0 0.0 0.36322757090784574 0.0 56-57 2.1949495673754402E-4 0.0 0.0 0.46876806260581483 0.0 58-59 2.1949495673754402E-4 0.0 0.0 0.5931851205832127 0.0 60-61 2.1949495673754402E-4 0.0 0.0 0.7335521454168721 0.0 62-63 2.1949495673754402E-4 0.0 0.0 0.890491039484216 0.0 64-65 2.1949495673754402E-4 0.0 0.0 1.068794109340681 0.0 66-67 2.1949495673754402E-4 0.0 0.0 1.2683516075078982 0.0 68-69 2.1949495673754402E-4 0.0 0.0 1.5123568344144678 0.0 70-71 2.1949495673754402E-4 0.0 0.0 1.7675928866074422 0.0 72-73 2.1949495673754402E-4 0.0 0.0 2.0445955220102228 0.0 74-75 2.926599423167254E-4 0.0 0.0 2.3338898749903056 0.0 76-77 2.926599423167254E-4 0.0 0.0 2.689288792441179 0.0 78-79 2.926599423167254E-4 0.0 0.0 3.0942569876219475 0.0 80-81 2.926599423167254E-4 0.0 0.0 3.566207727100457 0.0 82-83 2.926599423167254E-4 0.0 0.0 4.050011194242794 0.0 84-85 2.926599423167254E-4 0.0 0.0 4.595200084286064 0.0 86-87 2.926599423167254E-4 0.0 0.0 5.141705944069758 0.0 88-89 2.926599423167254E-4 0.0 0.0 5.827993508802479 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3865 0.0 54.091656 1 GTATCAA 7345 0.0 42.24239 1 ATCAACG 9330 0.0 33.04054 3 TATCAAC 9395 0.0 32.811947 2 TCAACGC 9395 0.0 32.811947 4 CAACGCA 9625 0.0 32.077217 5 AACGCAG 9865 0.0 31.295685 6 ACGCAGA 11510 0.0 26.58117 7 CGCAGAG 11565 0.0 26.33152 8 GCAGAGT 12320 0.0 24.255121 9 GAGTACT 7240 0.0 23.294497 12-13 GTACTTT 7335 0.0 22.793484 14-15 GTACATG 6875 0.0 22.461447 1 TGGTATC 1805 0.0 22.36793 2 TACATGG 6785 0.0 21.911825 2 AGTACTT 7285 0.0 21.71592 12-13 TAAGGTG 615 0.0 21.62554 5 GTGTAAG 515 0.0 21.220114 1 CAGAGTA 11945 0.0 20.840525 10-11 AGAGTAC 11160 0.0 20.752668 10-11 >>END_MODULE