FastQCFastQC Report
Thu 26 May 2016
SRR937147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879718
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76610.40756113417012546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53350.28381916861997386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35670.18976250692923086No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29620.15757682801356374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.048.4204861
GTATCAA69850.045.042711
ATCAACG86750.036.089983
TCAACGC87900.035.6197134
CAACGCA89400.035.0235
TATCAAC90450.034.7196352
AACGCAG92350.033.9998746
ACGCAGA106650.029.3089967
CGCAGAG107300.029.0871778
GCAGAGT115650.026.2476929
GTACATG74600.023.3808421
GAGTACT65400.023.06041512-13
TACATGG75300.022.9030822
ACATGGG74350.022.4922223
AGAGTAC106400.021.63041910-11
AGTACTT70150.021.49894712-13
GTACTTT70550.021.47690414-15
CAGAGTA114350.020.54200610-11
CATGGGG33900.020.0414984
CCTACAC9500.020.003553
Produced by FastQC (version 0.11.5)