##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937147_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1879718 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968982049435073 33.0 31.0 34.0 31.0 34.0 2 32.109786680768075 33.0 31.0 34.0 31.0 34.0 3 32.15634260032622 34.0 31.0 34.0 30.0 34.0 4 35.77846570602612 37.0 35.0 37.0 35.0 37.0 5 35.78856136931178 37.0 35.0 37.0 35.0 37.0 6 35.830313376793754 37.0 35.0 37.0 35.0 37.0 7 35.78087085403236 37.0 35.0 37.0 35.0 37.0 8 35.76415451679454 37.0 35.0 37.0 35.0 37.0 9 37.41199264996133 39.0 37.0 39.0 35.0 39.0 10-11 37.308351837882064 39.0 37.0 39.0 35.0 39.0 12-13 37.178662171666176 39.0 37.0 39.0 34.0 39.0 14-15 38.33748998519991 40.0 38.0 41.0 34.0 41.0 16-17 38.249363734347384 40.0 38.0 41.0 34.0 41.0 18-19 38.16400491988692 40.0 38.0 41.0 33.5 41.0 20-21 38.021528495231735 40.0 38.0 41.0 33.5 41.0 22-23 38.107577572806136 40.0 38.0 41.0 34.0 41.0 24-25 38.08177902217247 40.0 38.0 41.0 34.0 41.0 26-27 37.99554933239986 40.0 38.0 41.0 33.5 41.0 28-29 37.860836306297 40.0 38.0 41.0 33.0 41.0 30-31 37.6161762030262 40.0 37.0 41.0 33.0 41.0 32-33 37.37500704892968 39.5 37.0 41.0 32.5 41.0 34-35 37.37403589261794 40.0 37.0 41.0 33.0 41.0 36-37 37.234429313333166 40.0 37.0 41.0 32.0 41.0 38-39 37.0832630213681 39.5 36.5 41.0 31.0 41.0 40-41 36.85711127945788 39.0 36.0 41.0 31.0 41.0 42-43 36.59205263768288 39.0 36.0 41.0 30.0 41.0 44-45 36.348081999533974 39.0 36.0 40.5 30.0 41.0 46-47 35.97368860648246 39.0 35.0 40.0 29.5 41.0 48-49 35.632394061236845 39.0 35.0 40.0 28.0 41.0 50-51 34.68785477396078 38.0 34.0 39.5 26.5 40.5 52-53 34.8076269951131 38.0 34.5 39.5 27.0 40.5 54-55 35.106003400510076 38.0 35.0 40.0 27.0 41.0 56-57 34.824575814031675 38.0 34.0 40.0 26.0 41.0 58-59 34.539273710205464 38.0 34.0 40.0 25.5 41.0 60-61 34.19240332858439 38.0 34.0 40.0 24.5 41.0 62-63 33.792843926588986 37.0 33.0 40.0 23.0 41.0 64-65 33.34564493184616 37.0 33.0 39.5 22.0 41.0 66-67 32.89630146649657 36.0 32.0 39.0 20.5 40.5 68-69 32.36373993333042 35.5 31.5 39.0 18.5 40.0 70-71 31.86835312530922 35.0 31.0 38.0 16.0 40.0 72-73 31.341671463485483 35.0 31.0 37.5 10.5 39.5 74-75 30.75254639259719 34.5 30.0 37.0 7.5 39.0 76-77 30.185147718966356 34.0 29.5 36.5 4.5 39.0 78-79 29.588190356213005 34.0 29.0 36.0 2.0 38.5 80-81 28.96157349134285 34.0 28.5 35.5 2.0 37.0 82-83 28.351086439561676 33.5 27.5 35.0 2.0 37.0 84-85 27.696416962544383 33.0 26.0 35.0 2.0 36.0 86-87 27.043819870852968 33.0 25.5 35.0 2.0 36.0 88-89 26.400713298484135 32.0 24.5 35.0 2.0 35.5 90-91 25.757418931988735 32.0 22.0 34.0 2.0 35.0 92-93 25.00733328084319 31.5 19.0 34.0 2.0 35.0 94-95 24.156261205138215 31.0 12.0 34.0 2.0 35.0 96-97 23.20485306838579 31.0 2.0 34.0 2.0 35.0 98-99 21.926182810400284 30.0 2.0 34.0 2.0 35.0 100-101 18.773133523219972 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19479661725307906 1101 2 0.20984176570024005 1101 3 0.12085443029099707 1101 4 0.06752665229385002 1101 5 0.053690786126558976 1101 6 0.1805586675858848 1101 7 0.16948825011385793 1101 8 0.13482563241798573 1101 9 0.2565548258791992 1101 10-11 0.2779727477112104 1101 12-13 0.3813278149102999 1101 14-15 0.44371411161974095 1101 16-17 0.35660750559785015 1101 18-19 0.4837416007880151 1101 20-21 0.5051417044605913 1101 22-23 0.5211829463054372 1101 24-25 0.4788635023154626 1101 26-27 0.4465398038855568 1101 28-29 0.5043267027924969 1101 30-31 0.5604000357133287 1101 32-33 0.43129336482277836 1101 34-35 0.495795907281007 1101 36-37 0.39265793198414656 1101 38-39 0.4931457176593952 1101 40-41 0.5081188914734582 1101 42-43 0.6988683220295329 1101 44-45 0.5642494162036087 1101 46-47 0.8150067848473626 1101 48-49 0.6046388342840103 1101 50-51 0.6800352038231736 1101 52-53 0.5944426811527705 1101 54-55 0.7121097375846119 1101 56-57 0.7334116367186212 1101 58-59 0.7209231545447778 1101 60-61 0.842201444351744 1101 62-63 0.8520681880313674 1101 64-65 0.8171052472481577 1101 66-67 0.8532215466052264 1101 68-69 0.8621048216227081 1101 70-71 0.9095301569088541 1101 72-73 0.823511509539042 1101 74-75 0.7799848742867006 1101 76-77 0.9333206572951518 1101 78-79 1.0912621026001261 1101 80-81 1.4242023957025935 1101 82-83 1.4034059973751631 1101 84-85 1.480007611081053 1101 86-87 1.552881324215786 1101 88-89 1.6016320049389847 1101 90-91 1.7537013753628088 1101 92-93 1.7650973148794797 1101 94-95 1.7324091412834974 1101 96-97 1.8306382973043824 1101 98-99 2.2066180268718156 1101 100-101 2.417231710081783 1102 1 0.19224828232703928 1102 2 0.19193946284463692 1102 3 0.21724365720092464 1102 4 0.1604996270686243 1102 5 0.14399834256878563 1102 6 0.061852968394823904 1102 7 0.0795661105791865 1102 8 0.017913892681484356 1102 9 0.26909983371982804 1102 10-11 0.18244456300609357 1102 12-13 0.25581919480074333 1102 14-15 0.43102910369161407 1102 16-17 0.32539363192251614 1102 18-19 0.4622196081701091 1102 20-21 0.37209380880565845 1102 22-23 0.3936215124128779 1102 24-25 0.4213850288914216 1102 26-27 0.41668093105709403 1102 28-29 0.5345839092032563 1102 30-31 0.5179098008236025 1102 32-33 0.4768374557878303 1102 34-35 0.5979883669846302 1102 36-37 0.7016511459382357 1102 38-39 0.6315249708130963 1102 40-41 0.6961328631488541 1102 42-43 0.746813039494981 1102 44-45 0.719578471617595 1102 46-47 0.6605741883269971 1102 48-49 0.6846860718135375 1102 50-51 0.6156393302424092 1102 52-53 0.5707026663152632 1102 54-55 0.7594559130093046 1102 56-57 0.8247144021161787 1102 58-59 0.7467008789891878 1102 60-61 0.7188575130951236 1102 62-63 0.8254116088710077 1102 64-65 0.7987900274678097 1102 66-67 0.9759879775009139 1102 68-69 1.0339396165194543 1102 70-71 1.0653805668153673 1102 72-73 1.2961954507998392 1102 74-75 1.311146143762496 1102 76-77 1.384634585199688 1102 78-79 1.4801218248041188 1102 80-81 1.671727446239089 1102 82-83 1.6423094800231723 1102 84-85 1.7719419602066893 1102 86-87 1.6968353725204004 1102 88-89 1.8911176025341483 1102 90-91 2.0073435130558153 1102 92-93 1.9816682835696646 1102 94-95 1.9693339768615168 1102 96-97 1.8996710487831834 1102 98-99 1.98922955767069 1102 100-101 1.8938217996172533 1103 1 0.2333699680143475 1103 2 0.19380202527763402 1103 3 0.15548495363380255 1103 4 0.07369506840670681 1103 5 0.11530229565986616 1103 6 0.07799193318850683 1103 7 0.1039615799033129 1103 8 0.14246566572789732 1103 9 0.13608538675163118 1103 10-11 0.18600533195168367 1103 12-13 0.17095730655821484 1103 14-15 0.11631846222535813 1103 16-17 0.18536621718939728 1103 18-19 0.17324526156251352 1103 20-21 0.12783472591583944 1103 22-23 0.09690122031473436 1103 24-25 0.10107024383439978 1103 26-27 0.14003824641698515 1103 28-29 0.03922893042980036 1103 30-31 0.24839786545811648 1103 32-33 0.255430780246499 1103 34-35 0.3287106491555889 1103 36-37 0.36065680503963193 1103 38-39 0.274444035458167 1103 40-41 0.465538298361885 1103 42-43 0.2743734987724906 1103 44-45 0.4422988560365724 1103 46-47 0.5129138486732714 1103 48-49 0.47536535234859656 1103 50-51 0.39975876027926205 1103 52-53 0.3340263114811748 1103 54-55 0.5232524436918027 1103 56-57 0.4942780661271371 1103 58-59 0.6884474690695797 1103 60-61 0.3983747496635246 1103 62-63 0.5188229236376358 1103 64-65 0.4323194839196063 1103 66-67 0.6653915124779886 1103 68-69 0.6244292460541772 1103 70-71 0.6788380047383988 1103 72-73 0.7887146689541744 1103 74-75 0.6091548910578162 1103 76-77 0.781648730779704 1103 78-79 0.8016132864726551 1103 80-81 0.6960324577077763 1103 82-83 0.9006336209478185 1103 84-85 1.091329707493525 1103 86-87 1.3644415657992361 1103 88-89 1.2217632128752385 1103 90-91 1.083973943450978 1103 92-93 1.3256249639857884 1103 94-95 1.3977300638658292 1103 96-97 1.6723570819868065 1103 98-99 1.8126946358670537 1103 100-101 1.9298468992916682 1104 1 0.08529237611888973 1104 2 0.1577913552911454 1104 3 0.11773599672352475 1104 4 0.14285139858630913 1104 5 0.09072344867934845 1104 6 0.11234597212980901 1104 7 0.08653253875495182 1104 8 0.13383793124459942 1104 9 0.1346164359719566 1104 10-11 0.16524314914041582 1104 12-13 0.1780816750878671 1104 14-15 0.3559640372355801 1104 16-17 0.19539535465749225 1104 18-19 0.18973290875952387 1104 20-21 0.1357393142280543 1104 22-23 0.1853180774227141 1104 24-25 0.21587402710943593 1104 26-27 0.302529917838001 1104 28-29 0.27730914942146256 1104 30-31 0.17494637193576068 1104 32-33 0.2426928907003969 1104 34-35 0.3739515329287073 1104 36-37 0.3201400986102527 1104 38-39 0.47418265114306735 1104 40-41 0.35125565914983525 1104 42-43 0.27677903392951464 1104 44-45 0.15049932934140742 1104 46-47 0.4410380784734329 1104 48-49 0.31610154635954046 1104 50-51 0.2624940526554056 1104 52-53 0.20172593926290716 1104 54-55 0.3965028542691087 1104 56-57 0.47918669170265815 1104 58-59 0.4722149003017577 1104 60-61 0.5596108016317203 1104 62-63 0.4705381139182876 1104 64-65 0.630873341208634 1104 66-67 0.708726415513766 1104 68-69 0.580023304327824 1104 70-71 0.5366425218475577 1104 72-73 0.8416436033299952 1104 74-75 0.7137548958887656 1104 76-77 0.8622785669399669 1104 78-79 0.9893318148114112 1104 80-81 0.9819475240510371 1104 82-83 1.22209656937914 1104 84-85 0.9970258249275012 1104 86-87 1.3682757581937395 1104 88-89 1.4937922909183783 1104 90-91 1.2690466716914521 1104 92-93 1.4457376619238076 1104 94-95 1.4964487078481206 1104 96-97 1.4901851681461586 1104 98-99 1.6604645646575733 1104 100-101 1.489326473458398 1105 1 0.09771503671526105 1105 2 0.09170296796649069 1105 3 0.14543124567897792 1105 4 0.19748358624106288 1105 5 0.22175132834056654 1105 6 0.11964575239567665 1105 7 0.16989391491442518 1105 8 0.20665132164096178 1105 9 0.18567467441942398 1105 10-11 0.22875613899510938 1105 12-13 0.2050605956013456 1105 14-15 0.23411907497634843 1105 16-17 0.2321107590599567 1105 18-19 0.3515304909147474 1105 20-21 0.4092333595143458 1105 22-23 0.40058279693914045 1105 24-25 0.3470837913320963 1105 26-27 0.3581632948309519 1105 28-29 0.21919071278905733 1105 30-31 0.33042338315588893 1105 32-33 0.29149015568411585 1105 34-35 0.38837942960245897 1105 36-37 0.39422206513513913 1105 38-39 0.3780991367860551 1105 40-41 0.33430346852338744 1105 42-43 0.46324895461905413 1105 44-45 0.4023838402279978 1105 46-47 0.4879281382129008 1105 48-49 0.5844310592012576 1105 50-51 0.44875197617333384 1105 52-53 0.3125804582095597 1105 54-55 0.5166878863676061 1105 56-57 0.6409991103628911 1105 58-59 0.6741612739192391 1105 60-61 0.6282585410209336 1105 62-63 0.5421571107111802 1105 64-65 0.6141383130555482 1105 66-67 0.7821131544038096 1105 68-69 0.5490675410709684 1105 70-71 0.6883352351893457 1105 72-73 0.8673251539052238 1105 74-75 0.8578532968652048 1105 76-77 0.7351035627802496 1105 78-79 0.8595130553586365 1105 80-81 0.9228690006943125 1105 82-83 1.0786094324091842 1105 84-85 1.1815841443713033 1105 86-87 1.088805678588745 1105 88-89 1.2424591485180443 1105 90-91 1.3442972053110793 1105 92-93 1.3651220282918466 1105 94-95 1.4823184204749111 1105 96-97 1.738258345745276 1105 98-99 2.128594982592027 1105 100-101 2.129068327063198 1106 1 0.0327702315369649 1106 2 -0.5249145539452584 1106 3 -0.04753175546230892 1106 4 0.06307549355832265 1106 5 0.04074994020732703 1106 6 -0.014684682483405709 1106 7 0.06107869102650909 1106 8 0.05891636402687084 1106 9 0.1589453668727998 1106 10-11 -0.23053707329682993 1106 12-13 -0.17547558530805674 1106 14-15 -0.1843590678187823 1106 16-17 -0.07289673630484117 1106 18-19 -0.037705559686706636 1106 20-21 -0.012361149339334077 1106 22-23 -0.13667076087455143 1106 24-25 -0.06697375032692321 1106 26-27 -0.028271990101536915 1106 28-29 0.09746441679356366 1106 30-31 0.16931972709120657 1106 32-33 0.25974741287519976 1106 34-35 0.23978064448483138 1106 36-37 0.14782610371073446 1106 38-39 0.13510128208287853 1106 40-41 0.22180527707193676 1106 42-43 0.1667310057280531 1106 44-45 0.22615771364669968 1106 46-47 0.2804639289842825 1106 48-49 0.3141308239628202 1106 50-51 0.42502312579080126 1106 52-53 0.470215514606231 1106 54-55 0.5386408923024177 1106 56-57 0.3202174891915277 1106 58-59 0.24973884227775045 1106 60-61 0.19620576792085132 1106 62-63 0.4901639399754103 1106 64-65 0.5749854903561697 1106 66-67 0.592350186359532 1106 68-69 0.6680874857212942 1106 70-71 0.7231508926666699 1106 72-73 0.7122840068255023 1106 74-75 0.578676266194531 1106 76-77 0.7644077118795316 1106 78-79 0.7064920512339903 1106 80-81 0.8817392553055932 1106 82-83 0.9219754914189444 1106 84-85 0.8474506968316895 1106 86-87 0.7039461999222247 1106 88-89 0.8844098709436317 1106 90-91 1.0435675917557248 1106 92-93 1.1557368284824783 1106 94-95 1.1347005967146764 1106 96-97 0.8291015661959058 1106 98-99 0.860618934477472 1106 100-101 0.7503803325436174 1107 1 0.11697246220462532 1107 2 0.131365871185551 1107 3 0.014114342806806235 1107 4 0.08026856847159536 1107 5 0.047513825207715854 1107 6 0.09468061477587497 1107 7 0.0782950161889957 1107 8 0.039987754885835614 1107 9 0.1320598443186043 1107 10-11 0.10068379728937771 1107 12-13 0.09477271766593987 1107 14-15 0.19185515222739014 1107 16-17 0.21069091277527008 1107 18-19 0.27960366959658955 1107 20-21 0.21320622939850153 1107 22-23 0.2602395716330719 1107 24-25 0.3440039517153153 1107 26-27 0.3172838700753431 1107 28-29 0.1911517803799896 1107 30-31 0.16965491818403677 1107 32-33 0.1821870033995623 1107 34-35 0.17441450634154165 1107 36-37 0.1795624516972083 1107 38-39 0.23586132304206586 1107 40-41 0.19957077312084692 1107 42-43 0.28637799913177275 1107 44-45 0.27677883436683715 1107 46-47 0.20208370603376835 1107 48-49 0.3358359003641098 1107 50-51 0.1917090546430984 1107 52-53 0.30482971062713915 1107 54-55 0.33543129227452084 1107 56-57 0.22678660491673241 1107 58-59 0.4422213585747272 1107 60-61 0.49545681926806395 1107 62-63 0.5069772608181964 1107 64-65 0.39014455596740305 1107 66-67 0.5926875571632237 1107 68-69 0.5480604960569977 1107 70-71 0.35867126715067954 1107 72-73 0.5723831400755834 1107 74-75 0.657517134707593 1107 76-77 0.6598322081483516 1107 78-79 0.688546733882621 1107 80-81 0.797407210746492 1107 82-83 0.8857586266569228 1107 84-85 0.685503750025358 1107 86-87 0.6362458336339216 1107 88-89 0.767696768362562 1107 90-91 0.6286358942065924 1107 92-93 0.7228305085803619 1107 94-95 0.8134073403525832 1107 96-97 0.8366842959107892 1107 98-99 1.1222212932152544 1107 100-101 1.1588382888273365 1108 1 0.046540870599933726 1108 2 0.0675021729120715 1108 3 0.08544151331096117 1108 4 0.05521808888602919 1108 5 0.053252234023197786 1108 6 0.1583016522405316 1108 7 0.10524995823130467 1108 8 0.04809682761468537 1108 9 0.09206802113807555 1108 10-11 0.10449661705046509 1108 12-13 0.0072630454610376205 1108 14-15 0.09094260123438858 1108 16-17 0.14980075361527412 1108 18-19 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2316 26-27 -0.2648271558296642 2316 28-29 -0.0784545674275563 2316 30-31 -0.06681390556219213 2316 32-33 0.065187962321275 2316 34-35 0.020041173686088598 2316 36-37 -0.06494104857888772 2316 38-39 -0.02587275765705499 2316 40-41 -0.03714484233318416 2316 42-43 -0.2155366667214338 2316 44-45 -0.03532476307246668 2316 46-47 0.12435208548431831 2316 48-49 0.050162670944565946 2316 50-51 -0.12638109529610375 2316 52-53 0.049862100714797464 2316 54-55 0.02244538800004392 2316 56-57 0.136847091834305 2316 58-59 0.11958445096308878 2316 60-61 -0.12276876600606812 2316 62-63 0.15795270592592914 2316 64-65 0.3277186111329442 2316 66-67 0.2365710065133797 2316 68-69 0.3478802922119897 2316 70-71 0.4329230893137712 2316 72-73 0.43253556484824784 2316 74-75 0.6627382445429681 2316 76-77 0.7888008235868504 2316 78-79 0.7959879805090928 2316 80-81 0.8322457969654167 2316 82-83 0.9598727538757572 2316 84-85 1.1331630771984287 2316 86-87 1.2313065394511433 2316 88-89 1.3435850419786561 2316 90-91 1.3410737726028898 2316 92-93 1.628695183401394 2316 94-95 2.110398741126783 2316 96-97 2.3090771657135107 2316 98-99 2.344529427667659 2316 100-101 2.970750172536544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7097.0 3 1154.0 4 1078.0 5 1187.0 6 1335.0 7 1545.0 8 1963.0 9 2521.0 10 3264.0 11 4221.0 12 5736.0 13 7718.0 14 8762.0 15 11482.0 16 14269.0 17 18452.0 18 16522.0 19 12456.0 20 12760.0 21 13549.0 22 15288.0 23 17640.0 24 20097.0 25 22656.0 26 27051.0 27 31666.0 28 37700.0 29 45281.0 30 55353.0 31 68984.0 32 87713.0 33 114582.0 34 151955.0 35 205746.0 36 276095.0 37 329129.0 38 207083.0 39 18626.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73034188626981 19.591744621914824 14.822878776076816 22.855034715738547 2 14.809617902503675 20.32345890365298 40.96445685303552 23.90246634080782 3 18.851476319792134 25.121222270338993 29.64889806663012 26.378403343238755 4 12.216788911708912 16.84896315262613 38.28460864431466 32.6496392913503 5 13.200410661191183 37.75005666591112 34.6769243076759 14.372608365221797 6 30.964591498398132 37.10990961403225 17.897093392435472 14.02840549513415 7 27.8907360912875 32.87525012409567 21.314758347999994 17.919255436616837 8 25.89502980411393 35.072777487497206 20.135531408950833 18.89666129943804 9 25.85374983403567 17.057187175236468 20.83792123612898 36.251141754598876 10-11 24.163991021992278 27.214012645787307 27.887789834843268 20.734206497377148 12-13 25.212372444558675 24.44734640423897 28.37712312456108 21.96315802664127 14-15 22.35341163109677 25.81074974707183 26.633716024649 25.202122597182402 16-17 21.440991960870925 29.23012616770236 27.237977893927855 22.09090397749886 18-19 21.421766865226388 27.70422562241383 29.81213236509258 21.0618751472672 20-21 22.05719671787676 26.824789402778748 29.981575242158147 21.136438637186345 22-23 21.718167533196233 26.727796873563097 29.901654114094022 21.652381479146648 24-25 21.46990168437119 26.96286727749968 29.98612017788137 21.581110860247758 26-27 21.293302786651687 27.177110779700055 29.834778703152093 21.694807730496173 28-29 21.301957179729015 27.224439874664576 29.90981053067087 21.563792414935538 30-31 21.44608026106709 27.001247747123248 29.873438983032774 21.679233008776887 32-33 21.138468002247887 27.130402049971796 29.83413292612573 21.896997021654588 34-35 21.660626240464502 27.172747303135203 29.62377852985046 21.54284792654984 36-37 21.66991610114778 26.891696002209297 29.613117372558996 21.825270524083933 38-39 21.35776716832916 27.32822075424224 29.591192688720486 21.722819388708118 40-41 21.62743844306388 27.167116257139984 29.5951713423454 21.610273957450733 42-43 21.382532431217776 27.0868175156565 29.52068491405254 22.009965139073188 44-45 21.541341518935248 27.278584124012294 29.49660787263556 21.6834664844169 46-47 21.650197146408697 27.58194351624102 28.89872318010446 21.86913615724582 48-49 21.626508770241454 27.618296357571314 28.794761927738975 21.96043294444825 50-51 21.75549846374688 27.73936700612475 28.6646382366523 21.84049629347607 52-53 21.85905288290013 27.686421782232074 28.543796393128417 21.91072894173938 54-55 21.681068656624106 27.705698218816764 28.757815703225347 21.855417421333783 56-57 21.720527378847382 27.988735121292553 28.462103107863403 21.82863439199667 58-59 21.78188188393611 27.595950345977467 28.63248352999089 21.989684240095542 60-61 21.665012013448667 27.78514116968213 28.724762096403428 21.825084720465775 62-63 21.584535309802348 27.714055581794284 28.963883667797123 21.737525440606245 64-65 21.50222369340189 27.604127938261623 28.859348027539845 22.03430034079664 66-67 21.46676606193512 27.74668492170757 28.632180559660746 22.15436845669656 68-69 21.636662685563092 27.615674335954854 28.718904680333196 22.028758298148855 70-71 21.609400724596544 27.582831118577587 28.735239737081216 22.072528419744657 72-73 21.61177853124451 27.590093721938793 28.650585858736626 22.14754188808007 74-75 21.568237046703594 28.011627587747316 28.378148216560355 22.041987148988728 76-77 21.642325765386907 27.623768604875572 28.552106446704673 22.181799183032844 78-79 21.644183174420135 27.69975841141712 28.398454024996937 22.257604389165806 80-81 21.595810523588593 27.864618734083823 28.300636034039094 22.23893470828849 82-83 21.744777472359793 27.776034943632123 28.320589913672407 22.15859767033568 84-85 21.719211132117408 27.765053777482372 28.311836376166816 22.203898714233404 86-87 21.595709241121995 27.758518222399893 28.419055233526553 22.226717302951563 88-89 21.755745740169672 27.623823599180152 28.281968384103028 22.338462276547144 90-91 21.733733835115512 27.667112655785242 28.325381933709316 22.27377157538993 92-93 21.794797642411016 27.473673296463907 28.417620453547755 22.313908607577325 94-95 21.651678864800612 27.410059862364538 28.424974813030058 22.513286459804792 96-97 21.80191956260755 27.217082017481196 28.413770014990586 22.567228404920662 98-99 21.613281387441504 27.347001116324893 28.210820141614395 22.828897354619208 100-101 22.2071638436871 26.493418022720295 28.745271935659943 22.55414619793266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2766.0 1 1554.5 2 377.5 3 403.0 4 335.5 5 313.0 6 532.5 7 1079.5 8 1847.5 9 2213.0 10 2023.5 11 1831.5 12 1824.5 13 2028.5 14 2169.0 15 2178.5 16 2281.5 17 2436.0 18 2616.5 19 2833.0 20 3209.0 21 3758.5 22 4481.0 23 5485.5 24 6720.5 25 8550.0 26 11283.0 27 14463.5 28 17038.5 29 20073.0 30 24484.5 31 28988.0 32 33970.0 33 38673.5 34 43097.5 35 47787.0 36 53749.5 37 59213.0 38 61041.0 39 62077.5 40 63423.0 41 65273.5 42 66591.5 43 68113.5 44 70178.5 45 71629.0 46 71639.0 47 70520.5 48 70045.0 49 68892.5 50 66319.5 51 62217.0 52 58569.5 53 56668.5 54 54762.0 55 50383.5 56 44651.0 57 39484.5 58 35571.0 59 31423.5 60 25844.0 61 21052.0 62 17039.0 63 12861.0 64 9307.5 65 6897.0 66 5111.0 67 3809.0 68 2823.5 69 2088.0 70 1573.0 71 1161.0 72 872.5 73 682.0 74 541.0 75 397.5 76 287.5 77 195.0 78 131.0 79 96.5 80 60.0 81 37.0 82 27.5 83 18.0 84 9.5 85 7.5 86 7.0 87 4.0 88 3.0 89 3.0 90 3.5 91 2.5 92 3.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2847235595977694 2 0.24860111995522732 3 0.24737753216173916 4 0.247856327385278 5 0.24966510934086922 6 0.21785182671017672 7 0.22072459805140984 8 0.2201394038893068 9 0.22955570995223754 10-11 0.23774842822168007 12-13 0.23179008766208548 14-15 0.2226663786802063 16-17 0.21702723493630427 18-19 0.20620114293739808 20-21 0.211787087212018 22-23 0.20963250870609315 24-25 0.21138809119240226 26-27 0.2075045299348094 28-29 0.21870301821869026 30-31 0.2307792977457257 32-33 0.22237378159915477 34-35 0.22133639194815394 36-37 0.21317027341335246 38-39 0.20332837159616496 40-41 0.19957780901177732 42-43 0.2116806882734538 44-45 0.20769072807729672 46-47 0.21713363387486848 48-49 0.22037880150107622 50-51 0.23280087757844525 52-53 0.24338757196558208 54-55 0.22168218849848753 56-57 0.2021047838026768 58-59 0.19452385942997832 60-61 0.19659863873198002 62-63 0.20109399388631696 64-65 0.19537505093849186 66-67 0.19718383289408303 68-69 0.2006949978667013 70-71 0.20096099521311178 72-73 0.20197178512947156 74-75 0.20399336496219111 76-77 0.21279787712837778 78-79 0.21325007261727558 80-81 0.20614794346811596 82-83 0.2012269925595222 84-85 0.18944331011353832 86-87 0.19114569313056531 88-89 0.2001896029085214 90-91 0.19885961617646902 92-93 0.20314217345367763 94-95 0.19939161086928997 96-97 0.20609474399883387 98-99 0.2085419195858102 100-101 0.1960932437738001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1879718.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.34892227128127 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60165099355896 45.07719973223991 2 12.22805457404145 14.025315025913768 3 3.8144490571800027 6.562636274639343 4 1.725963482447505 3.959285823918076 5 0.8804001591407635 2.5245000147093655 6 0.5767674575084442 1.984619525355377 7 0.38114424394991747 1.5300748134301059 8 0.2770179390808739 1.2709344204879656 9 0.20819035234387778 1.0745543100779738 >10 1.1730270178198814 12.579559316272338 >50 0.08878163666171125 3.4766085959669124 >100 0.042697205479444965 4.270050517178809 >500 0.0012991165510038703 0.4919322306180631 >1k 3.7117615742967707E-4 0.4811293051728589 >5k 1.8558807871483853E-4 0.691600094019077 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7661 0.40756113417012546 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5335 0.28381916861997386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3567 0.18976250692923086 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2962 0.15757682801356374 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.319946928209444E-5 0.0 3 0.0 0.0 0.0 5.319946928209444E-5 0.0 4 0.0 0.0 0.0 5.319946928209444E-5 0.0 5 0.0 0.0 0.0 5.319946928209444E-5 0.0 6 0.0 0.0 0.0 5.319946928209444E-5 0.0 7 0.0 0.0 0.0 5.319946928209444E-5 0.0 8 0.0 0.0 0.0 5.319946928209444E-5 0.0 9 0.0 0.0 0.0 5.319946928209444E-5 0.0 10-11 0.0 0.0 0.0 5.319946928209444E-5 0.0 12-13 0.0 0.0 0.0 5.319946928209444E-5 0.0 14-15 0.0 0.0 0.0 5.319946928209444E-5 0.0 16-17 0.0 0.0 0.0 2.39397611769425E-4 0.0 18-19 0.0 0.0 0.0 3.723962849746611E-4 0.0 20-21 0.0 0.0 0.0 3.723962849746611E-4 0.0 22-23 0.0 0.0 0.0 4.2559575425675554E-4 0.0 24-25 0.0 0.0 0.0 4.2559575425675554E-4 0.0 26-27 0.0 0.0 0.0 5.053949581798972E-4 0.0 28-29 0.0 0.0 0.0 8.777912431545583E-4 0.0 30-31 0.0 0.0 0.0 0.0021279787712837777 0.0 32-33 0.0 0.0 0.0 0.00497415037787583 0.0 34-35 0.0 0.0 0.0 0.00832571694264778 0.0 36-37 0.0 0.0 0.0 0.01462985405257597 0.0 38-39 0.0 0.0 0.0 0.024684553746891824 0.0 40-41 0.0 0.0 0.0 0.03880901284128789 0.0 42-43 0.0 0.0 0.0 0.05402406105596691 0.0 44-45 0.0 0.0 0.0 0.07442605752565012 0.0 46-47 0.0 2.659973464104722E-5 0.0 0.09871161525292624 0.0 48-49 0.0 5.319946928209444E-5 0.0 0.1253379496286145 0.0 50-51 0.0 5.319946928209444E-5 0.0 0.1591196126227445 0.0 52-53 0.0 5.319946928209444E-5 0.0 0.19784882626010925 0.0 54-55 0.0 5.319946928209444E-5 0.0 0.24373336851591568 0.0 56-57 0.0 5.319946928209444E-5 0.0 0.2958954481470093 0.0 58-59 0.0 5.319946928209444E-5 0.0 0.3614371943025496 0.0 60-61 0.0 5.319946928209444E-5 0.0 0.43990641149363896 0.0 62-63 0.0 5.319946928209444E-5 0.0 0.5195460170089343 0.0 64-65 0.0 5.319946928209444E-5 0.0 0.6016327981112061 0.0 66-67 0.0 5.319946928209444E-5 0.0 0.7100001170388324 0.0 68-69 0.0 5.319946928209444E-5 0.0 0.8338484815275483 5.319946928209444E-5 70-71 0.0 5.319946928209444E-5 0.0 0.9759708637146636 5.319946928209444E-5 72-73 0.0 5.319946928209444E-5 0.0 1.1353830734184596 5.319946928209444E-5 74-75 0.0 1.0639893856418889E-4 0.0 1.2974818563210013 5.319946928209444E-5 76-77 0.0 1.0639893856418889E-4 0.0 1.4789186463075845 5.319946928209444E-5 78-79 0.0 1.0639893856418889E-4 0.0 1.6948286923889646 7.979920392314166E-5 80-81 0.0 1.0639893856418889E-4 0.0 1.939360052944112 1.0639893856418889E-4 82-83 0.0 1.0639893856418889E-4 0.0 2.196313489576628 1.0639893856418889E-4 84-85 0.0 1.0639893856418889E-4 0.0 2.459438064645867 1.0639893856418889E-4 86-87 0.0 1.0639893856418889E-4 0.0 2.7485505804594093 1.0639893856418889E-4 88-89 0.0 1.0639893856418889E-4 0.0 3.0703807698814396 1.0639893856418889E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3720 0.0 48.420486 1 GTATCAA 6985 0.0 45.04271 1 ATCAACG 8675 0.0 36.08998 3 TCAACGC 8790 0.0 35.619713 4 CAACGCA 8940 0.0 35.023 5 TATCAAC 9045 0.0 34.719635 2 AACGCAG 9235 0.0 33.999874 6 ACGCAGA 10665 0.0 29.308996 7 CGCAGAG 10730 0.0 29.087177 8 GCAGAGT 11565 0.0 26.247692 9 GTACATG 7460 0.0 23.380842 1 GAGTACT 6540 0.0 23.060415 12-13 TACATGG 7530 0.0 22.903082 2 ACATGGG 7435 0.0 22.492222 3 AGAGTAC 10640 0.0 21.630419 10-11 AGTACTT 7015 0.0 21.498947 12-13 GTACTTT 7055 0.0 21.476904 14-15 CAGAGTA 11435 0.0 20.542006 10-11 CATGGGG 3390 0.0 20.041498 4 CCTACAC 950 0.0 20.00355 3 >>END_MODULE