FastQCFastQC Report
Thu 26 May 2016
SRR937147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879718
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.41804142961869817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62770.3339330686837068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.23205608500849595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.049.157741
GTATCAA75950.040.4773251
ATCAACG97600.031.1947693
TCAACGC98650.030.9108894
CAACGCA100500.030.3891435
TATCAAC101650.030.045342
AACGCAG104050.029.4436176
ACGCAGA119300.025.6427827
CGCAGAG119650.025.448678
GCAGAGT128700.023.06869
GAGTACT80550.022.26235812-13
GGACCGA3500.021.7132846
GTACTTT82700.021.50898214-15
TAAGGTG8000.020.780295
AGAGTAC117750.020.39294610-11
TACATGG71000.020.203292
AGTACTT86200.019.83878112-13
GTACATG73600.019.6905271
TGGTATC22250.019.6395442
CAGAGTA127800.019.58842510-11
Produced by FastQC (version 0.11.5)