FastQCFastQC Report
Thu 26 May 2016
SRR937146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837417
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75170.4091069147613199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.2876320399778602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36330.19772321688544298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24850.1352442042280005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.050.0237661
GTATCAA64500.046.0771941
ATCAACG78400.037.588213
TCAACGC80750.036.49734
CAACGCA81700.036.0748835
TATCAAC82600.035.6769452
AACGCAG83950.035.1953246
ACGCAGA99800.029.46457
CGCAGAG100250.029.3330428
GCAGAGT109500.026.0308289
GTACATG71650.023.0292071
TACATGG70650.022.6722052
ACATGGG69200.022.1856653
GAGTACT65700.022.1614512-13
TGGTATC16900.021.0935942
AGTACTT69100.021.07101812-13
AGAGTAC102150.020.9278710-11
GTACTTT68950.020.83984814-15
ACCGCGC1604.986301E-520.7839898
CAGAGTA108550.020.32856810-11
Produced by FastQC (version 0.11.5)