FastQCFastQC Report
Thu 26 May 2016
SRR937146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837417
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80000.4353938164281706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.34260050930191677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.24610635473602346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.051.1096841
GTATCAA76050.040.4987681
ATCAACG97600.031.4430073
TATCAAC99000.031.0463432
TCAACGC99000.030.9983584
CAACGCA99900.030.81425
AACGCAG104300.029.6460726
ACGCAGA121050.025.3132637
CGCAGAG121900.025.1367558
TGGTATC20350.023.8109742
GGACCGA3650.023.423416
GCAGAGT130500.023.0797949
GAGTACT82350.021.95049312-13
GTACTTT84350.021.22886314-15
AGTACTT86450.020.38741312-13
AGAGTAC121200.020.14715410-11
CAGAGTA130100.019.64527910-11
GTACATG72500.019.077481
GTGGTAT26750.018.8342381
ACTTTTT98450.018.5985516-17
Produced by FastQC (version 0.11.5)