##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1837417 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.629470610101027 31.0 31.0 34.0 30.0 34.0 2 31.889066553754535 31.0 31.0 34.0 30.0 34.0 3 31.955856509436888 31.0 31.0 34.0 30.0 34.0 4 35.70922768212115 37.0 35.0 37.0 33.0 37.0 5 35.51196108450069 37.0 35.0 37.0 33.0 37.0 6 35.62934489013654 37.0 35.0 37.0 33.0 37.0 7 35.5799227937915 37.0 35.0 37.0 33.0 37.0 8 35.54816843427486 37.0 35.0 37.0 33.0 37.0 9 37.15198019828923 39.0 37.0 39.0 33.0 39.0 10-11 37.04327705686842 39.0 37.0 39.0 33.0 39.0 12-13 36.94142837472387 39.0 37.0 39.0 33.0 39.0 14-15 37.98027285041991 40.0 37.0 41.0 33.0 41.0 16-17 37.935869756293755 40.0 37.0 41.0 33.0 41.0 18-19 37.934047633172 40.0 37.0 41.0 33.0 41.0 20-21 37.82248749195202 39.5 37.0 41.0 32.5 41.0 22-23 37.65932964591053 39.0 37.0 41.0 32.0 41.0 24-25 37.49005152341575 39.0 36.0 40.5 32.0 41.0 26-27 37.20623244478526 39.0 36.0 40.0 31.5 41.0 28-29 37.18732955012389 39.0 36.0 40.0 31.0 41.0 30-31 37.32576138133042 39.0 36.0 40.0 32.0 41.0 32-33 37.27438137341714 39.0 36.0 40.0 32.0 41.0 34-35 37.13489480069032 39.0 36.0 40.0 31.0 41.0 36-37 36.93367509933782 39.0 36.0 40.0 31.0 41.0 38-39 36.76730812874813 39.0 35.0 40.0 30.5 41.0 40-41 36.570574344310515 39.0 35.0 40.0 30.0 41.0 42-43 36.33556019129027 38.5 35.0 40.0 30.0 41.0 44-45 36.016225494811465 38.0 35.0 40.0 29.5 41.0 46-47 35.68076435561443 38.0 35.0 40.0 28.5 41.0 48-49 35.348117221077196 38.0 34.5 40.0 27.0 41.0 50-51 35.099870905733425 38.0 34.0 40.0 27.0 41.0 52-53 34.822213193847666 38.0 34.0 40.0 26.0 41.0 54-55 34.582442363382945 38.0 34.0 40.0 26.0 41.0 56-57 34.29690293493529 38.0 33.0 40.0 25.0 41.0 58-59 34.001274343276464 37.0 33.0 40.0 24.0 41.0 60-61 33.614704500938004 37.0 32.5 39.5 23.0 40.5 62-63 33.18073360592615 36.0 32.0 39.0 22.5 40.0 64-65 32.780197690562346 36.0 31.0 39.0 22.0 40.0 66-67 32.33781553125937 35.0 31.0 38.5 20.5 40.0 68-69 31.83741360834258 35.0 30.5 38.0 19.0 40.0 70-71 31.43117430610471 35.0 30.0 37.5 18.0 39.5 72-73 30.945562711131984 34.0 29.5 37.0 17.5 39.0 74-75 30.437371864960433 34.0 29.0 36.0 13.0 39.0 76-77 29.30214997466552 32.5 28.5 35.0 13.0 37.5 78-79 29.96436111127741 34.0 29.0 36.0 12.0 38.0 80-81 29.758067167115577 34.0 29.0 35.0 8.0 37.0 82-83 29.34363919567523 34.0 29.0 35.0 7.0 37.0 84-85 28.915116710033704 33.5 29.0 35.0 4.0 36.0 86-87 28.477018009520975 33.0 28.0 35.0 2.0 36.0 88-89 27.97540297058316 33.0 27.0 35.0 2.0 35.0 90-91 27.34110710851157 32.0 26.0 34.0 2.0 35.0 92-93 26.72402807854722 32.0 25.0 34.0 2.0 35.0 94-95 26.104766092835757 32.0 24.5 34.0 2.0 35.0 96-97 25.426046999674 31.5 22.5 34.0 2.0 35.0 98-99 24.436019695039285 31.0 18.0 34.0 2.0 35.0 100-101 22.559872364302713 30.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05405081146300006 1101 2 0.06502134007937599 1101 3 0.07274412983334955 1101 4 0.07810799456735396 1101 5 -0.08737041816367963 1101 6 0.06625636885078023 1101 7 -0.006648587918029136 1101 8 -0.03442469210455812 1101 9 -0.015905064913916078 1101 10-11 0.08338102872428976 1101 12-13 0.11837821207663524 1101 14-15 0.19677141111963437 1101 16-17 0.08216063358290882 1101 18-19 0.1452300758402103 1101 20-21 0.017201508465653603 1101 22-23 -0.11858491905837809 1101 24-25 -0.09104377053318302 1101 26-27 -0.059230396547100383 1101 28-29 0.021055997075741573 1101 30-31 0.19237151201407698 1101 32-33 0.21017943292061858 1101 34-35 0.19418516676026343 1101 36-37 0.3126652776085095 1101 38-39 0.18609671156946916 1101 40-41 0.14373012783674 1101 42-43 0.15229237300042797 1101 44-45 0.0919173863349414 1101 46-47 0.11798767090737527 1101 48-49 0.1873674388747233 1101 50-51 0.3105459597583362 1101 52-53 0.4291718653233332 1101 54-55 0.25933599760702464 1101 56-57 0.06232406406487456 1101 58-59 -0.04502229826740489 1101 60-61 -0.07886262958583501 1101 62-63 -0.006284840141482562 1101 64-65 0.08154544458521684 1101 66-67 0.08670804792732412 1101 68-69 -0.18681663267044613 1101 70-71 -0.27882770754643005 1101 72-73 -0.21198295033137882 1101 74-75 -0.407864622580842 1101 76-77 -0.16096963166685185 1101 78-79 -0.10337032469237073 1101 80-81 -0.20331536839831443 1101 82-83 -0.17761599142923146 1101 84-85 -0.18545141113545682 1101 86-87 -0.12490418427565331 1101 88-89 -0.03760026459760368 1101 90-91 0.2638721324555391 1101 92-93 0.14493065558927043 1101 94-95 -0.23387265871947704 1101 96-97 0.1893579743421725 1101 98-99 -0.12574513849509472 1101 100-101 0.10042753061715715 1102 1 0.17136205574986363 1102 2 0.18275679446319515 1102 3 0.06955460539629499 1102 4 0.1377329409658543 1102 5 -0.05414230064827308 1102 6 0.09228985802066347 1102 7 0.017568411862335154 1102 8 0.1495881513372339 1102 9 0.2177874246011129 1102 10-11 0.25517189398320284 1102 12-13 0.09729959306039859 1102 14-15 0.14098297230901835 1102 16-17 0.1349042981473474 1102 18-19 0.1508071417203709 1102 20-21 0.08391168386020809 1102 22-23 0.056748665286846744 1102 24-25 0.1587893065588446 1102 26-27 0.11402397262863673 1102 28-29 0.16490746078883234 1102 30-31 0.1729278171190316 1102 32-33 0.24941928524194878 1102 34-35 0.31818514584260527 1102 36-37 0.2737182724941434 1102 38-39 0.13816818720562907 1102 40-41 0.26270108366910705 1102 42-43 0.4855095087455652 1102 44-45 0.3936695539272179 1102 46-47 0.4782087705486333 1102 48-49 0.6036548684083627 1102 50-51 0.420939107133691 1102 52-53 0.3887699325317655 1102 54-55 0.4962339089789083 1102 56-57 0.4944651955009789 1102 58-59 0.43256432698229474 1102 60-61 0.42127615907261173 1102 62-63 0.33579071443791264 1102 64-65 0.3505547843193497 1102 66-67 0.2735900618793963 1102 68-69 0.2250164862568944 1102 70-71 0.13818797023797558 1102 72-73 0.3375939900428335 1102 74-75 0.1654666189867271 1102 76-77 0.12447858144224355 1102 78-79 0.2452284014222812 1102 80-81 0.22264480438154166 1102 82-83 0.1651574807973475 1102 84-85 0.17605309139035086 1102 86-87 0.3010778474564333 1102 88-89 0.5417783763821795 1102 90-91 0.29937033876639774 1102 92-93 0.4018014785945141 1102 94-95 0.3745250487052445 1102 96-97 0.7412948448514136 1102 98-99 0.5477381744435235 1102 100-101 0.6516321785206856 1103 1 0.1274376120812235 1103 2 0.050252443785336 1103 3 0.06587263598063586 1103 4 0.029429678883140298 1103 5 -0.1927190868065125 1103 6 -0.007843333551981857 1103 7 0.056293835160808214 1103 8 -0.043074357721174295 1103 9 -0.06814642393711523 1103 10-11 0.012203700215380309 1103 12-13 -0.003245868217248926 1103 14-15 0.059603873547310116 1103 16-17 -0.06616292709875182 1103 18-19 -0.04311396951415958 1103 20-21 -0.04528742114320039 1103 22-23 0.07648228214155495 1103 24-25 -0.02863408712857307 1103 26-27 -0.02355128484157376 1103 28-29 -0.20733145886776327 1103 30-31 -0.1377521581257497 1103 32-33 0.0844099491368766 1103 34-35 0.22489804342050945 1103 36-37 0.058713749905003 1103 38-39 -0.02073242691437116 1103 40-41 -0.07620132890739484 1103 42-43 -0.06303442913363 1103 44-45 -0.10026784920739118 1103 46-47 -0.1526455491851877 1103 48-49 -0.23242629487562283 1103 50-51 -0.38263262747760507 1103 52-53 -0.2169666147491256 1103 54-55 -0.33375662267657447 1103 56-57 -0.27342346946244334 1103 58-59 -0.3825886211639684 1103 60-61 -0.5302212675477165 1103 62-63 -0.5634917014181227 1103 64-65 -0.5380471273341172 1103 66-67 -0.49122049188651573 1103 68-69 -0.2756854407363498 1103 70-71 -0.32522264382913235 1103 72-73 -0.4624832377951549 1103 74-75 -0.33473618343166933 1103 76-77 -0.44055212455772264 1103 78-79 -0.17456911353400173 1103 80-81 -0.2969830209142792 1103 82-83 -0.2920879185922338 1103 84-85 -0.25259346430097196 1103 86-87 -0.2624464925364798 1103 88-89 -0.3742773969276634 1103 90-91 -0.20307968794597286 1103 92-93 -0.23159046326244948 1103 94-95 -0.18792085213632248 1103 96-97 -0.002981461542102437 1103 98-99 0.026251803041098754 1103 100-101 0.16719062571218402 1104 1 0.016754656821390768 1104 2 0.12844795994546132 1104 3 0.05526103295706619 1104 4 0.07040746124375374 1104 5 -0.09765639422389683 1104 6 -0.06435757206789106 1104 7 -0.004814698104119941 1104 8 0.05047939312989058 1104 9 -0.06344943967767591 1104 10-11 0.06941363894750907 1104 12-13 0.03231302686907611 1104 14-15 0.09579609133733413 1104 16-17 -0.003526887366398057 1104 18-19 0.04999030256042403 1104 20-21 -0.1335843813851767 1104 22-23 0.004256018111121307 1104 24-25 -0.08294103159158084 1104 26-27 -0.25843020139085837 1104 28-29 -0.13436663660230153 1104 30-31 -0.12528461193615215 1104 32-33 -0.07247829985208654 1104 34-35 0.040804500065910077 1104 36-37 0.1256710236427736 1104 38-39 -0.15660963826614704 1104 40-41 -0.12010427713597238 1104 42-43 0.13813379134838755 1104 44-45 0.12811956267111668 1104 46-47 -0.1246217090939794 1104 48-49 -0.2381676781330242 1104 50-51 -0.02649479693779 1104 52-53 -0.05765540251855583 1104 54-55 0.0901904438511707 1104 56-57 0.023111480933643236 1104 58-59 0.026625152724342627 1104 60-61 -0.18352408257696595 1104 62-63 -0.005805444605663013 1104 64-65 0.09753686727510313 1104 66-67 -0.11036567809293985 1104 68-69 -0.11042651146969007 1104 70-71 -0.01763212801165892 1104 72-73 0.027449477236999087 1104 74-75 0.08279162176887667 1104 76-77 -0.013112553833824592 1104 78-79 -0.10638985583912941 1104 80-81 0.013583581189543281 1104 82-83 0.06083163542128034 1104 84-85 -0.07422210296267906 1104 86-87 -0.026084518801656742 1104 88-89 0.13224975203598532 1104 90-91 0.20579185829708635 1104 92-93 0.17331735274595417 1104 94-95 -0.13081604285132542 1104 96-97 -0.06895745638385975 1104 98-99 0.15167102129810317 1104 100-101 0.5054475391667772 1105 1 0.05678695565946157 1105 2 0.014838674871306523 1105 3 -0.02689573684831359 1105 4 0.1388152088206951 1105 5 -0.06115702362387054 1105 6 0.05554182754151782 1105 7 0.07816378833140192 1105 8 0.14360274516391058 1105 9 0.0057003782366535916 1105 10-11 0.12003015305590736 1105 12-13 -0.08655485611950553 1105 14-15 0.024027334959150437 1105 16-17 0.14711306735824792 1105 18-19 0.04740535946543645 1105 20-21 0.05260631126560611 1105 22-23 -0.006017058592789226 1105 24-25 -0.11416723483338131 1105 26-27 0.057706008015436794 1105 28-29 -0.041935171265926385 1105 30-31 0.04320032131636253 1105 32-33 0.08988131546279732 1105 34-35 -0.02036870925231682 1105 36-37 0.1228549120395428 1105 38-39 -0.07750370999183787 1105 40-41 0.06327159897325174 1105 42-43 -0.1586742701515007 1105 44-45 0.14701386491778834 1105 46-47 0.15587472689630744 1105 48-49 0.015436154731489182 1105 50-51 0.05804261998400051 1105 52-53 0.20762405883940005 1105 54-55 0.28258512943610015 1105 56-57 0.2245071688963023 1105 58-59 0.11965072760221318 1105 60-61 0.19982194236074236 1105 62-63 0.2050587629979077 1105 64-65 0.34345127146903565 1105 66-67 0.15692339521273624 1105 68-69 0.12038488351894117 1105 70-71 0.05516846875276116 1105 72-73 0.20881477010224359 1105 74-75 0.09063354395120982 1105 76-77 0.1466796017186205 1105 78-79 0.08439506808894492 1105 80-81 0.2214979620723554 1105 82-83 0.1481453407999318 1105 84-85 0.0564347264155316 1105 86-87 0.27228635836951653 1105 88-89 0.327949111239473 1105 90-91 0.211324361754901 1105 92-93 0.3642155945702328 1105 94-95 0.5590749647584587 1105 96-97 0.31767964402486015 1105 98-99 0.41468444849750696 1105 100-101 0.38455158960166 1106 1 -0.0920748114244887 1106 2 0.07032189528747423 1106 3 -0.013360314315065125 1106 4 0.057118012697976894 1106 5 -0.1276849834011955 1106 6 0.02670473494944048 1106 7 -0.01666107389106486 1106 8 0.05755312666594392 1106 9 -0.06595462496963478 1106 10-11 -0.05653570135064001 1106 12-13 -0.2782949421781922 1106 14-15 -0.040874362051098956 1106 16-17 0.020995396518756593 1106 18-19 0.028600701516161564 1106 20-21 -0.11036742968475721 1106 22-23 -0.11025284636427557 1106 24-25 -0.08671082178889122 1106 26-27 0.034082156935987484 1106 28-29 -0.04552122065208408 1106 30-31 0.046572037192618154 1106 32-33 -0.028855150741051716 1106 34-35 0.14472118303493176 1106 36-37 -0.01853294680935136 1106 38-39 0.17475242610354513 1106 40-41 0.1652840105679374 1106 42-43 -0.10971538501102884 1106 44-45 0.13010719118798875 1106 46-47 0.09150160304057664 1106 48-49 -0.31463268756551344 1106 50-51 0.10559898563116832 1106 52-53 0.06780646885417951 1106 54-55 -0.09510803791364708 1106 56-57 -0.04760205871310319 1106 58-59 0.008649435792655424 1106 60-61 0.16553787436880896 1106 62-63 0.3001802070365258 1106 64-65 0.19767323501955758 1106 66-67 0.14621206353366034 1106 68-69 0.33046413464114366 1106 70-71 0.17426680427008634 1106 72-73 0.14358640326550542 1106 74-75 0.19784618602702508 1106 76-77 0.21259871492389237 1106 78-79 0.23078671407736806 1106 80-81 0.29728443245831926 1106 82-83 -3.7116926967684094E-4 1106 84-85 -0.08023338075819098 1106 86-87 0.31866034652948017 1106 88-89 0.30027185916932453 1106 90-91 0.298917413864082 1106 92-93 0.14529767094498425 1106 94-95 -0.06820219809318928 1106 96-97 0.17975980591926444 1106 98-99 0.19354918953151312 1106 100-101 0.24799965235200716 1107 1 -0.11220698462141243 1107 2 -0.01576209370487547 1107 3 0.004408972713360271 1107 4 -0.04147227552864763 1107 5 -0.1648490890775065 1107 6 -0.18704477278260612 1107 7 -0.06604587361432124 1107 8 -0.16562518266147208 1107 9 -0.03452656312282443 1107 10-11 -0.20573700033953912 1107 12-13 -0.0943307278138974 1107 14-15 -0.09209752072955979 1107 16-17 -0.21308079758264853 1107 18-19 -0.17235702149469034 1107 20-21 -0.12314688526333128 1107 22-23 -0.194125130810761 1107 24-25 -0.24199350030111333 1107 26-27 -0.12210856891535826 1107 28-29 -0.25966262753892266 1107 30-31 -0.2602544774066473 1107 32-33 -0.2690706593660579 1107 34-35 -0.1995941844633009 1107 36-37 -0.11409948288287097 1107 38-39 -0.16807530465191434 1107 40-41 -0.19734815365145408 1107 42-43 -0.2587185798150742 1107 44-45 -0.3032068703787232 1107 46-47 -0.28525880102014156 1107 48-49 -0.28854134896775463 1107 50-51 -0.18963993040976135 1107 52-53 -0.30083034692329846 1107 54-55 -0.31299941936416076 1107 56-57 -0.10694605256529144 1107 58-59 -0.11504550052073625 1107 60-61 -0.17004044022542075 1107 62-63 -0.07866908894548175 1107 64-65 -0.14174123843628195 1107 66-67 -0.10465402881042252 1107 68-69 -0.3445877854133492 1107 70-71 -0.3767924845418733 1107 72-73 -0.24608117671531815 1107 74-75 -0.29882434696300564 1107 76-77 -0.22978959561249823 1107 78-79 -0.35087386582325664 1107 80-81 -0.34475243766313923 1107 82-83 -0.31858921314945476 1107 84-85 -0.3832316367231634 1107 86-87 -0.2548594082283131 1107 88-89 -0.21777249941002097 1107 90-91 0.10640395654506207 1107 92-93 0.057664878298421485 1107 94-95 -0.03668675494430218 1107 96-97 -0.03677225763207659 1107 98-99 0.19839705629058813 1107 100-101 0.26390125382384255 1108 1 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-0.563817662559579 2316 54-55 -0.37847373719800714 2316 56-57 -0.25850998357615396 2316 58-59 -0.2064950322269894 2316 60-61 -0.26469252325289006 2316 62-63 -0.28750374944124957 2316 64-65 -0.4942572541058823 2316 66-67 -0.3466997931930642 2316 68-69 -0.2631449885612547 2316 70-71 -0.2711717943241929 2316 72-73 -0.296774936604951 2316 74-75 -0.020388231365874532 2316 76-77 -0.03930508228144802 2316 78-79 -0.0759310188675748 2316 80-81 -0.24318839013231397 2316 82-83 -0.3650107684113806 2316 84-85 -0.3100116540147262 2316 86-87 -0.2682935779419786 2316 88-89 -0.32183648285486655 2316 90-91 -0.4271619506444573 2316 92-93 -0.2882240641896523 2316 94-95 -0.2920938640605897 2316 96-97 -0.5662663745388947 2316 98-99 -0.6983015834553044 2316 100-101 -0.7441665887623437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 26.0 5 54.0 6 116.0 7 279.0 8 662.0 9 1222.0 10 1912.0 11 3277.0 12 4798.0 13 6582.0 14 8787.0 15 11618.0 16 15064.0 17 19304.0 18 17067.0 19 12396.0 20 11412.0 21 12262.0 22 13239.0 23 14841.0 24 17837.0 25 21481.0 26 26622.0 27 32822.0 28 40381.0 29 49922.0 30 61610.0 31 75717.0 32 95167.0 33 120352.0 34 157186.0 35 210404.0 36 284097.0 37 325271.0 38 157197.0 39 6418.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.64806133682659 21.662044190652907 16.048267578748153 21.641626893772344 2 16.235073475427733 22.303157094987146 38.71625221710695 22.74551721247817 3 19.755559026611813 25.866202391727082 30.48654714743578 23.89169143422533 4 13.76845865690804 18.61428298529947 37.28141189506791 30.335846462724575 5 13.257057634056265 38.24172175517569 34.60754029920612 13.893680311561917 6 30.938975746931696 38.2449928350505 17.113752621206835 13.702278796810957 7 27.64538479833375 33.42142801552397 21.136247242732598 17.79693994340969 8 26.089287298419467 35.93386803322273 19.575033865475284 18.401810802882526 9 25.854174637548255 17.047572761109752 20.610563633622633 36.48768896771936 10-11 24.44719951976062 27.69929199523026 27.484207449914745 20.369301035094374 12-13 25.479563592980327 24.82031587830437 28.19707928089885 21.503041247816455 14-15 22.403733066582056 26.265023127575287 26.580438735463968 24.75080507037869 16-17 21.344338274871735 29.6531489585652 27.26183005817406 21.740682708389006 18-19 21.382979476079736 27.860578192103368 30.048894725584884 20.70754760623201 20-21 22.023198871023833 26.911555732857593 30.39533214289407 20.6699132532245 22-23 21.824194292780202 26.804759136863552 30.275085556049007 21.095961014307235 24-25 21.655451592678382 27.135018460788242 30.228071283024782 20.981458663508594 26-27 21.45391412251651 27.297735640597914 30.154649134503657 21.093701102381917 28-29 21.336491106042274 27.36970377784345 30.281064629843403 21.01274048627087 30-31 21.457097777891466 27.180568836708847 30.206395018175147 21.15593836722454 32-33 21.37612202347099 27.238563701108674 30.081195504341146 21.30411877107918 34-35 21.7684390641863 27.264197512050885 29.94929839007694 21.018065033685875 36-37 21.844729017175503 26.960480044366715 29.92198000069119 21.272810937766597 38-39 21.524535181947442 27.355180178647963 29.923492955446857 21.196791683957738 40-41 21.799058623888488 27.199440083399708 29.89088211627576 21.110619176436042 42-43 21.578186786121538 27.269864769613033 29.711114800229694 21.440833644035738 44-45 21.785963665297533 27.358841242896958 29.62520211797322 21.22999297383229 46-47 21.959141555781837 27.698421207597406 28.979349815529083 21.363087421091674 48-49 21.925731046825803 27.811603955880443 28.830806733258356 21.4318582640354 50-51 22.014545418922324 27.90308351343217 28.66915893343754 21.413212134207967 52-53 22.202100343199735 27.89540081875941 28.479999608144922 21.42249922989593 54-55 22.01500802485228 27.935982958686022 28.664478449910934 21.38453056655076 56-57 22.07465697770294 28.13933908307151 28.492089710718904 21.293914228506647 58-59 22.028233112026285 27.803323905243065 28.611877434463707 21.556565548266942 60-61 21.893614786409398 28.004992878589892 28.717025041131112 21.384367293869598 62-63 21.858728856868094 27.94107162391553 28.87333142122882 21.326868097987557 64-65 21.767377791758758 27.822045839349478 28.799804290479514 21.61077207841225 66-67 21.73281840757977 27.947874652297223 28.56716793193924 21.75213900818377 68-69 21.85750431175939 27.896443757731642 28.59563180268823 21.65042012782074 70-71 21.934160835564274 27.78724154614875 28.606788769234203 21.671808849052773 72-73 21.967849432110402 27.753335252694406 28.547167028497068 21.73164828669812 74-75 21.875328246119416 28.228159421622856 28.164700772878447 21.73181155937928 76-77 21.90686708569693 27.84800619565401 28.557562055864295 21.687564662784766 78-79 21.982163003825477 27.86593897846814 28.318802971780492 21.833095045925884 80-81 21.884444304150882 28.08412570472571 28.217709970028576 21.81372002109483 82-83 21.97821664977244 28.010993720789422 28.2063499622431 21.804439667195037 84-85 21.91610056307397 28.037121737398323 28.201673656562974 21.84510404296473 86-87 21.81252268809965 28.08412570472571 28.207668700137205 21.895682907037433 88-89 21.874348610032452 27.93611901925366 28.20930142694881 21.98023094376508 90-91 21.85136804750475 28.037027360950095 28.123676719516244 21.987927872028912 92-93 21.899912347993432 27.887298187445765 28.242886132124585 21.96990333243622 94-95 21.69828623551431 27.822698930074118 28.23425493505285 22.24475989935872 96-97 21.804250205587515 27.718095565677253 28.141951445970076 22.335702782765154 98-99 21.616858696302906 27.777009780177313 28.09672960013933 22.509401923380448 100-101 21.63540279260248 27.770299655292725 28.208001149568556 22.386296402536235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 357.0 1 261.0 2 197.5 3 261.5 4 255.0 5 266.0 6 481.0 7 1000.0 8 1716.5 9 2158.0 10 2086.5 11 1907.5 12 1963.5 13 2103.5 14 2310.5 15 2456.5 16 2545.5 17 2640.5 18 2853.5 19 3213.5 20 3518.0 21 3986.5 22 4688.5 23 5709.5 24 6995.5 25 8758.5 26 11503.0 27 14546.0 28 17181.0 29 20595.0 30 24776.0 31 28974.0 32 33928.5 33 38712.0 34 43126.5 35 47400.5 36 53120.0 37 58469.5 38 60933.5 39 62055.5 40 62605.5 41 64544.0 42 65863.0 43 67366.5 44 69707.0 45 70629.0 46 69813.5 47 68654.5 48 68093.0 49 66743.5 50 64232.5 51 60403.5 52 56701.5 53 54537.0 54 52861.5 55 48455.0 56 42733.0 57 37912.5 58 33922.5 59 29464.5 60 24057.5 61 19718.5 62 15754.5 63 11517.0 64 8242.0 65 6098.5 66 4489.0 67 3331.0 68 2441.5 69 1778.5 70 1298.0 71 924.5 72 708.0 73 590.0 74 449.0 75 337.0 76 247.5 77 151.5 78 83.0 79 49.0 80 30.5 81 18.5 82 10.5 83 5.0 84 4.0 85 3.5 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040219503792552264 2 0.0 3 0.0 4 0.0 5 0.012082178405881735 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005197513683611287 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013606056763380333 24-25 4.89818043481692E-4 26-27 0.0017415752657126825 28-29 0.002503514444461981 30-31 9.79636086963384E-4 32-33 0.0 34-35 0.0 36-37 1.9048479468732464E-4 38-39 2.44909021740846E-4 40-41 2.7212113526760664E-5 42-43 0.009905209323740881 44-45 0.0 46-47 0.0 48-49 2.7212113526760664E-5 50-51 0.0 52-53 1.6327268116056397E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.44909021740846E-4 84-85 1.6327268116056397E-4 86-87 0.0 88-89 0.0 90-91 0.006911876835797209 92-93 0.00204090851450705 94-95 0.0 96-97 0.0 98-99 0.0014694541304450758 100-101 0.011211390773025394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1837417.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.767650553826236 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67504428424613 45.44872465519135 2 12.361096716351252 14.28143031144456 3 3.7965320369912923 6.579502080879572 4 1.6428817217580352 3.7962166881514623 5 0.8652750985167835 2.499245476202256 6 0.5378545157670885 1.8642355029378357 7 0.3682066767087962 1.4889304242189587 8 0.2525428448198408 1.1671045447537383 9 0.20797178546957565 1.0812637285255584 >10 1.1623382800348077 12.505573798186528 >50 0.08866086404450763 3.511839856272239 >100 0.03961488422512177 4.0316546806424896 >500 0.0014144936192444408 0.5025831092575862 >1k 3.7719829846518413E-4 0.4629800396249973 >5k 1.8859914923259206E-4 0.7787151037109468 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8000 0.4353938164281706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6295 0.34260050930191677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4522 0.24610635473602346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.442422705352133E-5 0.0 5 0.0 0.0 0.0 5.442422705352133E-5 0.0 6 0.0 0.0 0.0 5.442422705352133E-5 0.0 7 0.0 0.0 0.0 5.442422705352133E-5 0.0 8 0.0 0.0 0.0 5.442422705352133E-5 0.0 9 0.0 0.0 0.0 1.63272681160564E-4 0.0 10-11 0.0 0.0 0.0 1.63272681160564E-4 0.0 12-13 0.0 0.0 0.0 2.1769690821408532E-4 0.0 14-15 0.0 0.0 0.0 2.1769690821408532E-4 0.0 16-17 0.0 0.0 0.0 3.26545362321128E-4 0.0 18-19 0.0 0.0 0.0 4.3539381642817063E-4 0.0 20-21 0.0 0.0 0.0 5.170301570084526E-4 0.0 22-23 0.0 0.0 0.0 7.34727065222538E-4 0.0 24-25 0.0 0.0 0.0 8.707876328563413E-4 0.0 26-27 0.0 0.0 0.0 0.0011701208816507086 0.0 28-29 0.0 0.0 0.0 0.0016599389251324005 0.0 30-31 0.0 0.0 0.0 0.0031293930555774765 0.0 32-33 0.0 0.0 0.0 0.005551271159459175 0.0 34-35 0.0 0.0 0.0 0.009197694372045104 0.0 36-37 0.0 0.0 0.0 0.014667329190923999 0.0 38-39 0.0 0.0 0.0 0.024572538514664878 0.0 40-41 0.0 0.0 0.0 0.037389443985769155 0.0 42-43 0.0 0.0 0.0 0.05205677317669315 0.0 44-45 0.0 0.0 0.0 0.06977185908261435 0.0 46-47 0.0 0.0 0.0 0.09162318624460317 0.0 48-49 0.0 0.0 0.0 0.1158147551698934 0.0 50-51 0.0 0.0 0.0 0.14841486717495267 0.0 52-53 0.0 0.0 0.0 0.18392667532737533 0.0 54-55 0.0 0.0 0.0 0.2258877543856403 0.0 56-57 0.0 0.0 0.0 0.275495437344925 0.0 58-59 0.0 0.0 0.0 0.33792002577531394 0.0 60-61 0.0 0.0 0.0 0.4112838838434607 0.0 62-63 0.0 0.0 0.0 0.4879676197618722 0.0 64-65 0.0 0.0 0.0 0.5710734144725993 0.0 66-67 0.0 0.0 0.0 0.6772278693404926 0.0 68-69 0.0 0.0 0.0 0.7969339567447128 0.0 70-71 0.0 0.0 0.0 0.9311713127722232 0.0 72-73 0.0 0.0 0.0 1.0784976954061054 0.0 74-75 0.0 0.0 0.0 1.2357564994772554 0.0 76-77 0.0 0.0 0.0 1.42044511398338 0.0 78-79 0.0 0.0 0.0 1.6317471755186763 0.0 80-81 0.0 0.0 0.0 1.8686558358826548 0.0 82-83 0.0 0.0 0.0 2.121646855340949 0.0 84-85 0.0 0.0 0.0 2.379726540028747 0.0 86-87 0.0 0.0 0.0 2.660011309354382 0.0 88-89 0.0 0.0 0.0 2.9698484339700784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4315 0.0 51.109684 1 GTATCAA 7605 0.0 40.498768 1 ATCAACG 9760 0.0 31.443007 3 TATCAAC 9900 0.0 31.046343 2 TCAACGC 9900 0.0 30.998358 4 CAACGCA 9990 0.0 30.8142 5 AACGCAG 10430 0.0 29.646072 6 ACGCAGA 12105 0.0 25.313263 7 CGCAGAG 12190 0.0 25.136755 8 TGGTATC 2035 0.0 23.810974 2 GGACCGA 365 0.0 23.42341 6 GCAGAGT 13050 0.0 23.079794 9 GAGTACT 8235 0.0 21.950493 12-13 GTACTTT 8435 0.0 21.228863 14-15 AGTACTT 8645 0.0 20.387413 12-13 AGAGTAC 12120 0.0 20.147154 10-11 CAGAGTA 13010 0.0 19.645279 10-11 GTACATG 7250 0.0 19.07748 1 GTGGTAT 2675 0.0 18.834238 1 ACTTTTT 9845 0.0 18.59855 16-17 >>END_MODULE