FastQCFastQC Report
Thu 26 May 2016
SRR937145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901499
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76680.4032607958247677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.27830674641427633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.19647656927508245No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26720.1405207154986671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.048.410651
GTATCAA68400.043.040581
ATCAACG83450.034.6322143
TCAACGC84700.034.1790354
CAACGCA85950.033.6257635
TATCAAC86950.033.3483772
AACGCAG88550.032.616946
ACGCAGA103650.027.6818967
CGCAGAG104750.027.2090858
GCAGAGT114400.024.2489059
GTACATG77050.023.9498441
TACATGG76000.023.7675512
ACATGGG77200.022.8433423
GAGTACT65750.021.744412-13
CATGGGG35600.021.0975974
AGAGTAC104900.020.71671510-11
GTACTTT69650.020.3563514-15
CAGAGTA111450.019.96801210-11
AGTACTT70600.019.74604212-13
TGGTATC19500.019.745352
Produced by FastQC (version 0.11.5)