##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1901499 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.951562425223468 33.0 31.0 34.0 31.0 34.0 2 32.0740668283286 33.0 31.0 34.0 30.0 34.0 3 32.13461747810543 34.0 31.0 34.0 30.0 34.0 4 35.75763121621416 37.0 35.0 37.0 35.0 37.0 5 35.74968590569861 37.0 35.0 37.0 35.0 37.0 6 35.82361494799629 37.0 35.0 37.0 35.0 37.0 7 35.778758758221805 37.0 35.0 37.0 35.0 37.0 8 35.76681975641323 37.0 35.0 37.0 35.0 37.0 9 37.435557946651564 39.0 37.0 39.0 35.0 39.0 10-11 37.35796653061611 39.0 37.0 39.0 35.0 39.0 12-13 37.26186945141701 39.0 37.0 39.0 34.0 39.0 14-15 38.49087036069964 40.0 38.0 41.0 34.0 41.0 16-17 38.43152376099067 40.0 38.0 41.0 34.0 41.0 18-19 38.38643722663015 40.0 38.0 41.0 34.0 41.0 20-21 38.310760615703714 40.0 38.0 41.0 34.0 41.0 22-23 38.18225883894759 40.0 38.0 41.0 34.0 41.0 24-25 38.04631556472026 40.0 38.0 41.0 33.5 41.0 26-27 37.88674566749707 40.0 38.0 41.0 33.0 41.0 28-29 37.71488888503228 40.0 37.0 41.0 33.0 41.0 30-31 37.72455073602458 40.0 37.0 41.0 33.0 41.0 32-33 37.58772578896965 40.0 37.0 41.0 33.0 41.0 34-35 37.401478254787406 40.0 37.0 41.0 32.5 41.0 36-37 37.427597647960894 40.0 37.0 41.0 33.0 41.0 38-39 37.41459369686758 40.0 37.0 41.0 33.0 41.0 40-41 37.269863407764085 40.0 37.0 41.0 32.5 41.0 42-43 37.05522485155133 40.0 37.0 41.0 31.5 41.0 44-45 36.85454554538288 40.0 36.5 41.0 31.0 41.0 46-47 36.571432590813885 39.5 36.0 41.0 30.5 41.0 48-49 36.29214267270191 39.0 36.0 41.0 30.0 41.0 50-51 35.398994161974315 38.5 35.0 40.0 29.0 40.5 52-53 35.54758824485314 38.5 35.0 40.0 29.0 40.5 54-55 35.911088304542886 39.0 35.5 40.0 29.0 41.0 56-57 35.714655385040956 39.0 35.0 40.5 28.5 41.0 58-59 35.49282040116771 39.0 35.0 40.0 28.0 41.0 60-61 35.22644766050364 38.0 35.0 40.0 27.5 41.0 62-63 34.926338378300485 38.0 34.5 40.0 27.0 41.0 64-65 34.583894863999404 37.5 34.0 40.0 26.0 41.0 66-67 34.20808188697444 37.0 34.0 40.0 26.0 41.0 68-69 33.72261200242546 36.5 33.5 39.0 25.5 41.0 70-71 33.301449014698406 36.0 33.0 39.0 24.5 40.0 72-73 32.89240593868311 35.5 33.0 38.5 23.5 40.0 74-75 32.431448031263756 35.0 32.0 37.5 22.0 39.5 76-77 31.990936361260246 35.0 32.0 37.0 21.0 39.0 78-79 31.523997909018096 35.0 31.5 36.5 19.5 39.0 80-81 31.079505958194034 35.0 31.0 36.0 18.0 38.5 82-83 30.63241263866034 34.0 31.0 35.5 15.5 37.0 84-85 30.172518102823087 34.0 30.0 35.0 9.5 37.0 86-87 29.751141862288648 34.0 30.0 35.0 7.0 36.0 88-89 29.373384892655743 34.0 29.5 35.0 2.0 36.0 90-91 28.949838522134378 34.0 29.0 35.0 2.0 35.5 92-93 28.518614787596523 34.0 29.0 35.0 2.0 35.0 94-95 27.99995687612773 34.0 28.0 35.0 2.0 35.0 96-97 27.40185821817419 33.0 27.0 35.0 2.0 35.0 98-99 26.706892825081685 33.0 25.5 35.0 2.0 35.0 100-101 24.82770487915061 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -2.2800165579894838E-4 1101 2 -0.004235321317246132 1101 3 0.06675959116740415 1101 4 -0.03000079632030861 1101 5 0.017702691060769382 1101 6 -0.03966694141069382 1101 7 -0.06293887148851951 1101 8 -0.03968597802327878 1101 9 0.013866750487579793 1101 10-11 -0.03410059119707398 1101 12-13 0.04811000700173196 1101 14-15 0.023757986593572866 1101 16-17 0.10927218544079409 1101 18-19 0.2817081867387614 1101 20-21 0.25744716816245017 1101 22-23 0.20288628485036497 1101 24-25 0.19443891593047624 1101 26-27 0.26718361212661534 1101 28-29 0.21112207906576685 1101 30-31 0.16134917911956137 1101 32-33 0.007169236282429381 1101 34-35 0.03224607363112142 1101 36-37 -0.10886272629880267 1101 38-39 -0.001706015273043704 1101 40-41 0.1182425036905741 1101 42-43 0.1296277387383995 1101 44-45 0.13410880268481407 1101 46-47 0.21514392637758561 1101 48-49 0.1821893913815984 1101 50-51 0.009051328327508656 1101 52-53 0.10420823440919236 1101 54-55 0.11383971948142602 1101 56-57 0.17218613370948077 1101 58-59 0.17768546454359324 1101 60-61 0.15671945735525128 1101 62-63 0.10400528402600884 1101 64-65 0.15931035750659817 1101 66-67 0.18280841790677727 1101 68-69 0.09501826848475758 1101 70-71 0.08494186791646996 1101 72-73 0.12851367469534836 1101 74-75 0.04624912555929228 1101 76-77 0.08410241561620069 1101 78-79 0.24064355287080375 1101 80-81 0.26373842450047746 1101 82-83 0.38787113820928454 1101 84-85 0.27792427810582154 1101 86-87 0.5216059338599521 1101 88-89 0.49160214132338 1101 90-91 0.48457635792910736 1101 92-93 0.4802296415111549 1101 94-95 0.38982631126987144 1101 96-97 0.37916778417629615 1101 98-99 0.6325387489962289 1101 100-101 0.9884206300762237 1102 1 0.09227028100189116 1102 2 -0.045684329669079204 1102 3 0.007823024445691829 1102 4 -0.09374259291624298 1102 5 -0.09630283355974001 1102 6 0.005917210858804367 1102 7 -0.08254257836824763 1102 8 -0.06348224692808913 1102 9 0.0328563332618117 1102 10-11 -0.0314068290087306 1102 12-13 0.07537695085638774 1102 14-15 0.14934615813058372 1102 16-17 0.19749770795596788 1102 18-19 0.27941801559535406 1102 20-21 0.17079637885071008 1102 22-23 0.19259211274665944 1102 24-25 0.34874591890082485 1102 26-27 0.3023951624862491 1102 28-29 0.03851513472013579 1102 30-31 0.18796517638678978 1102 32-33 0.04744242165180168 1102 34-35 0.03926762164826414 1102 36-37 0.12491093734674763 1102 38-39 0.2247923996959429 1102 40-41 0.07804317132169558 1102 42-43 -0.10138631912824536 1102 44-45 -0.08382932139904398 1102 46-47 -0.01196257963487568 1102 48-49 -0.13831942042556022 1102 50-51 -0.10093472073668153 1102 52-53 -0.13132209046495547 1102 54-55 0.06404022517684638 1102 56-57 -0.041180281607339 1102 58-59 -0.02185296460516639 1102 60-61 0.002407669542620283 1102 62-63 -0.01748716653305138 1102 64-65 -0.11078129926792712 1102 66-67 -0.04072537119318298 1102 68-69 -0.10554453298630762 1102 70-71 -0.2290226318823514 1102 72-73 -0.21991770837787072 1102 74-75 -0.3551296579507266 1102 76-77 -0.17195488266213133 1102 78-79 -0.09898080788466501 1102 80-81 -0.18520335561896317 1102 82-83 -0.1904229240510844 1102 84-85 -0.13838643790213823 1102 86-87 -0.16934942439367262 1102 88-89 -0.17199920974965366 1102 90-91 -0.03257168467512983 1102 92-93 -0.21315641662343054 1102 94-95 -0.18976652361603996 1102 96-97 0.018289153989265117 1102 98-99 0.3530746263677891 1102 100-101 0.45717196931197535 1103 1 -0.007765119003899201 1103 2 -0.12675666436063793 1103 3 0.008390117856436063 1103 4 -0.06842282034089209 1103 5 -0.08616224088697066 1103 6 -0.12336622042950296 1103 7 -0.02547148838657165 1103 8 -0.03524462479705193 1103 9 0.07176799515750076 1103 10-11 0.08044495426656084 1103 12-13 0.09620799861907159 1103 14-15 0.009699762589356453 1103 16-17 0.03279408308510057 1103 18-19 0.12209904400629767 1103 20-21 0.13981495039522684 1103 22-23 0.029166640158628354 1103 24-25 0.11420589262814929 1103 26-27 -0.04255872574353248 1103 28-29 -0.06436001459814378 1103 30-31 -0.08294570491430164 1103 32-33 -0.038155750761724505 1103 34-35 -0.04053165868756281 1103 36-37 0.01467917334570501 1103 38-39 0.0471829645340307 1103 40-41 0.14895921172501403 1103 42-43 -0.177005197282476 1103 44-45 -0.19710159108183944 1103 46-47 -0.1157300701129671 1103 48-49 -0.07490222687113146 1103 50-51 -0.12957844374060556 1103 52-53 -0.11970279305719345 1103 54-55 0.0024081217761988682 1103 56-57 0.09746957114413846 1103 58-59 0.11022871886903118 1103 60-61 0.0026813262694176387 1103 62-63 0.06982686618422207 1103 64-65 -0.1302867840257349 1103 66-67 0.20432647880377175 1103 68-69 0.06785646139496038 1103 70-71 -0.04289364290733033 1103 72-73 0.18748548096689177 1103 74-75 0.06482371029049006 1103 76-77 0.08873924293529001 1103 78-79 0.12658441968476097 1103 80-81 0.21294016285613182 1103 82-83 0.29463454182237214 1103 84-85 0.14532238155253197 1103 86-87 0.37241146895891575 1103 88-89 0.32823580468971514 1103 90-91 0.3247217361032888 1103 92-93 0.226104228969902 1103 94-95 0.4790966019353 1103 96-97 0.494477042374843 1103 98-99 0.5466778901830729 1103 100-101 0.6440043702684264 1104 1 0.10025407061310787 1104 2 0.03799713627571322 1104 3 -0.03035233980032359 1104 4 -0.030085948443073107 1104 5 0.033940216702688986 1104 6 0.03708635791313952 1104 7 0.044405862815466435 1104 8 0.07244867016152767 1104 9 0.014491484654634235 1104 10-11 0.04136066373126113 1104 12-13 0.12950923181788454 1104 14-15 0.037415488495589955 1104 16-17 0.07540260768406881 1104 18-19 0.11394203072809006 1104 20-21 0.05887605501454374 1104 22-23 0.0883832095322461 1104 24-25 0.07822787730564329 1104 26-27 0.14704380860123933 1104 28-29 0.010832733008392381 1104 30-31 -0.21429320592721268 1104 32-33 -0.10986893326357716 1104 34-35 0.058921366764096206 1104 36-37 -0.16387335105864764 1104 38-39 -0.058897005065915664 1104 40-41 -0.09134489790309885 1104 42-43 -0.2066974721949677 1104 44-45 0.008191491814521612 1104 46-47 -0.03302958198400319 1104 48-49 0.025452146870065917 1104 50-51 -0.033680060798864986 1104 52-53 -0.08747004974558337 1104 54-55 0.014746610983927155 1104 56-57 0.009056489388619582 1104 58-59 -0.05820324548304967 1104 60-61 0.01627557945055713 1104 62-63 0.20912921673613738 1104 64-65 0.13049898700244 1104 66-67 0.09958783625359757 1104 68-69 0.14044435858169635 1104 70-71 0.21981876533256184 1104 72-73 0.1645730312876239 1104 74-75 0.2100980699724957 1104 76-77 0.06370826826280052 1104 78-79 0.11330368807012903 1104 80-81 0.15130895313683723 1104 82-83 0.06851901321452658 1104 84-85 0.09664118538072586 1104 86-87 0.14232019328894552 1104 88-89 0.15304414759296847 1104 90-91 0.25084661792977414 1104 92-93 0.4082705350692919 1104 94-95 0.37331065055358437 1104 96-97 0.35937462253360053 1104 98-99 0.48807389371632226 1104 100-101 0.6109887667509462 1105 1 0.17369302681068532 1105 2 0.19211756452566675 1105 3 0.15768212022740613 1105 4 0.19452069352269064 1105 5 0.20959679843159762 1105 6 0.00367467878236738 1105 7 0.12849878668971826 1105 8 0.04400369027835893 1105 9 0.206726612970499 1105 10-11 0.2560259513906544 1105 12-13 0.17968607813434545 1105 14-15 0.23923896041157633 1105 16-17 0.1923275730194618 1105 18-19 0.28068151200920255 1105 20-21 0.18840598022991628 1105 22-23 0.2412304806890191 1105 24-25 0.3766069376106955 1105 26-27 0.25021772263301045 1105 28-29 0.2853739100138242 1105 30-31 0.24378126046516257 1105 32-33 0.12709331072978358 1105 34-35 0.2790659352181635 1105 36-37 0.3471318088531561 1105 38-39 0.39540254258939456 1105 40-41 0.4584384767246803 1105 42-43 0.3249859774447472 1105 44-45 0.4588043165821034 1105 46-47 0.2879139772185937 1105 48-49 0.38246621729035724 1105 50-51 0.2725604774685877 1105 52-53 0.16963926309963995 1105 54-55 0.38134447882654143 1105 56-57 0.44667156451420453 1105 58-59 0.4197007392417973 1105 60-61 0.48278275104603807 1105 62-63 0.6037047173839696 1105 64-65 0.4969606968199116 1105 66-67 0.6272642847794927 1105 68-69 0.6191341501394447 1105 70-71 0.573616642267524 1105 72-73 0.49365197566513785 1105 74-75 0.43794636401927534 1105 76-77 0.65260809323496 1105 78-79 0.5984067726517672 1105 80-81 0.4371069209953582 1105 82-83 0.5955205816458644 1105 84-85 0.6451759522754585 1105 86-87 0.5936601791957941 1105 88-89 0.6354158885517585 1105 90-91 0.7553880222440945 1105 92-93 0.6762311197077544 1105 94-95 0.5677431739095731 1105 96-97 0.6038485161075826 1105 98-99 0.9795698135217457 1105 100-101 1.2157986228644155 1106 1 0.06402381561475678 1106 2 0.20637809949742802 1106 3 0.030759759400744713 1106 4 0.04067473063557969 1106 5 0.06030988724187125 1106 6 0.09493515169243949 1106 7 0.05657549493771796 1106 8 -0.003195465682665599 1106 9 0.13284837800494387 1106 10-11 0.05843580678693172 1106 12-13 -0.0014459270788407252 1106 14-15 -0.015565855575516707 1106 16-17 -0.04563742418776684 1106 18-19 -5.926144725236782E-4 1106 20-21 0.06589248181249019 1106 22-23 0.15940462578925008 1106 24-25 0.1343032077031623 1106 26-27 0.2925388298135516 1106 28-29 0.27612113275322514 1106 30-31 0.276626168303558 1106 32-33 0.3105023593240972 1106 34-35 0.34072608540746074 1106 36-37 0.11423290734505542 1106 38-39 0.14265010666808564 1106 40-41 0.3258207146692058 1106 42-43 0.31983796031571643 1106 44-45 0.29224253417038426 1106 46-47 0.23798721001198686 1106 48-49 0.17471036797613948 1106 50-51 0.3959242210296807 1106 52-53 0.37130810192542896 1106 54-55 0.5691313589854232 1106 56-57 0.47890950903105534 1106 58-59 0.38201501963651197 1106 60-61 0.396126014249667 1106 62-63 0.6433491039041783 1106 64-65 0.6191446476670563 1106 66-67 0.5530062322753082 1106 68-69 0.44300928202053313 1106 70-71 0.4433938408028695 1106 72-73 0.6338915337859135 1106 74-75 0.5302985020444808 1106 76-77 0.46741787415719216 1106 78-79 0.4709823327585134 1106 80-81 0.6223852676719552 1106 82-83 0.5566494837745815 1106 84-85 0.7181176763491877 1106 86-87 0.5890955419463673 1106 88-89 0.6905496314922424 1106 90-91 0.7364784461254352 1106 92-93 0.8425012121796733 1106 94-95 0.7878499200152014 1106 96-97 0.8028809743710958 1106 98-99 0.9415861978077267 1106 100-101 1.2839144299766865 1107 1 0.0016078792538394282 1107 2 -0.04165330641961873 1107 3 0.06502633300190297 1107 4 0.02522290127046034 1107 5 0.09593920583245108 1107 6 0.11307701750822474 1107 7 0.04957563896767425 1107 8 0.054912867004382804 1107 9 0.13881860442742067 1107 10-11 0.18033796243530276 1107 12-13 0.18519400171866351 1107 14-15 0.2135680812064109 1107 16-17 0.25131172585083306 1107 18-19 0.1784273909193672 1107 20-21 0.20095934495078893 1107 22-23 0.17076969741645343 1107 24-25 0.1691204189138702 1107 26-27 0.19146968435437373 1107 28-29 0.07552211585002055 1107 30-31 0.09951031282100331 1107 32-33 0.09623711523024525 1107 34-35 0.1863071354966408 1107 36-37 0.1542093455211173 1107 38-39 0.19715953693727784 1107 40-41 0.16357576772023208 1107 42-43 0.15489559076439008 1107 44-45 0.17199192204306257 1107 46-47 0.11922564606823016 1107 48-49 0.15452623121414888 1107 50-51 0.215723539287886 1107 52-53 0.18944775189570606 1107 54-55 0.24989781994775484 1107 56-57 0.19148554023015407 1107 58-59 0.13673532453685056 1107 60-61 0.1678606889081422 1107 62-63 0.19452220944858567 1107 64-65 0.0912914695906295 1107 66-67 0.16228286950816084 1107 68-69 0.10864410329413232 1107 70-71 0.24094976320395745 1107 72-73 0.4111100567771544 1107 74-75 0.3158397407759779 1107 76-77 0.29130173430779394 1107 78-79 0.3846079361147794 1107 80-81 0.41721606940179257 1107 82-83 0.2731579021409871 1107 84-85 0.4234216718879509 1107 86-87 0.5151860149185943 1107 88-89 0.6187407260091327 1107 90-91 0.6319392865956353 1107 92-93 0.5218798152368009 1107 94-95 0.4636783692557209 1107 96-97 0.6004524076907671 1107 98-99 0.5268013379414924 1107 100-101 0.6869793136263311 1108 1 -0.1097360919632635 1108 2 -0.19600410634621568 1108 3 -0.22024050382642102 1108 4 -0.15779123994147426 1108 5 -0.05314687038440269 1108 6 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-0.7287214563852693 2316 88-89 -0.6284578560680387 2316 90-91 -0.599934789807552 2316 92-93 -0.8267244039651267 2316 94-95 -0.934308476018689 2316 96-97 -0.9848315412812276 2316 98-99 -1.1850930272243083 2316 100-101 -1.368634662644908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6702.0 3 1119.0 4 890.0 5 1055.0 6 1134.0 7 1415.0 8 1597.0 9 2052.0 10 2685.0 11 3471.0 12 4829.0 13 6215.0 14 7391.0 15 9620.0 16 12041.0 17 16096.0 18 13566.0 19 9194.0 20 8726.0 21 9077.0 22 9902.0 23 11009.0 24 12348.0 25 14113.0 26 16416.0 27 19736.0 28 23777.0 29 29900.0 30 37861.0 31 48632.0 32 64649.0 33 89032.0 34 127092.0 35 187390.0 36 284589.0 37 410547.0 38 347622.0 39 47999.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73662843418441 19.578800886013113 14.78475040394412 22.899820275858357 2 14.892486046599926 20.31928290281346 40.88309307751045 23.905137973076158 3 18.82600423145154 25.067658927286956 29.638248862507353 26.468087978754145 4 12.193479422204854 16.867827593826316 38.24588937177356 32.69280361219527 5 13.274638659707112 37.6979785191626 34.680231439176026 14.347151381954262 6 31.03380860462898 37.06984364370093 17.902271911671942 13.994075839998146 7 27.942277764280494 32.84688651020474 21.24394137341693 17.966894352097835 8 25.86661284398255 35.12762774236149 20.13971413198538 18.866045281670583 9 25.872796632811745 17.031924945470795 20.807206128560864 36.288072293156596 10-11 24.170672937565417 27.217195571722353 27.87729480005186 20.734836690660373 12-13 25.234602359146358 24.42536519758457 28.36641078153706 21.973621661732015 14-15 22.325991305493346 25.81388486365433 26.631958898695824 25.2281649321565 16-17 21.46626326526418 29.23148554621769 27.221241708304106 22.081009480214025 18-19 21.44071532019394 27.72216161045053 29.774191364170598 21.06293170518493 20-21 22.04512668908905 26.8619337261861 29.953745604778526 21.139193979946327 22-23 21.72550654179408 26.69596848008743 29.899363499692527 21.679161478425964 24-25 21.481747863382967 27.00275573540725 29.939589853835376 21.57590654737441 26-27 21.273179985771876 27.235554501620424 29.76217953784945 21.729085974758252 28-29 21.352625131842192 27.313091050224003 29.84198435046691 21.492299467466893 30-31 21.442237788182606 27.06819677193848 29.84242824765951 21.64713719221941 32-33 21.184732817990753 27.12017551441153 29.81041450154624 21.884677166051475 34-35 21.697042640537347 27.159553762615012 29.603525761195392 21.53987783565225 36-37 21.64775240741507 26.95187751896278 29.605678993935125 21.794691079687027 38-39 21.334413836055138 27.35098915527941 29.58795646936954 21.72664053929591 40-41 21.722456197108663 27.155447903104363 29.600574747467828 21.521521152319146 42-43 21.392578705924723 27.115208966103914 29.453525956404004 22.038686371567366 44-45 21.551140366917846 27.35102031997411 29.435162793246803 21.662676519861236 46-47 21.721180281567683 27.554956020692735 28.830085877221524 21.893777820518057 48-49 21.60003477935529 27.65014076417094 28.762474129561006 21.987350326912765 50-51 21.73762273967174 27.773184043104866 28.66508881103255 21.824104406190845 52-53 21.8963464541532 27.70274009350607 28.501193991621243 21.899719460719496 54-55 21.672014674543615 27.748769325755735 28.71507358221136 21.86414241748929 56-57 21.7396435674864 27.991464587257468 28.519474703302205 21.749417141953924 58-59 21.784282039834217 27.61338448591263 28.63548782623927 21.966845648013887 60-61 21.679984573812046 27.805730133382788 28.74863479817141 21.76565049463376 62-63 21.525669073958703 27.745307361097755 28.99229605082051 21.73672751412303 64-65 21.476640363944327 27.659136738594142 28.83089206664059 22.033330830820944 66-67 21.423238438476414 27.80327100095369 28.67110317247048 22.102387388099416 68-69 21.63121669650393 27.69596970402629 28.685341035175654 21.987472564294123 70-71 21.650373156936663 27.61032036438452 28.707925995590085 22.031380483088732 72-73 21.632660911481757 27.633845461534527 28.65501929372784 22.078474333255873 74-75 21.5283059478174 28.02040010400797 28.444730486848446 22.006563461326188 76-77 21.626695929505715 27.72860385431638 28.5497900795287 22.094910136649204 78-79 21.628704469206333 27.80108535685098 28.407231707721902 22.162978466220785 80-81 21.556156822724212 27.973499871050407 28.32265163563139 22.14769167059399 82-83 21.725604425772463 27.881202006752055 28.307407604517458 22.085785962958028 84-85 21.650392652090122 27.88746537359637 28.30314026841324 22.159001705900266 86-87 21.523039852183306 27.87761393215532 28.424471418075566 22.174874797585808 88-89 21.665218561945075 27.762248779297778 28.31304669465926 22.25948596409789 90-91 21.63350511688781 27.784729792600473 28.392733982426126 22.189031108085594 92-93 21.687977949448342 27.67602950963236 28.43121137346462 22.204781167454676 94-95 21.496714309161867 27.529592030563 28.46338528772419 22.510308372550945 96-97 21.654434747834728 27.489717311350564 28.392760376011967 22.463087564802738 98-99 21.474629270596964 27.52762143829862 28.300760888723087 22.69698840238133 100-101 21.63581759928604 27.28611358648824 28.54186955173737 22.536199262488346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2504.0 1 1440.0 2 414.0 3 443.0 4 380.5 5 352.5 6 552.5 7 1157.5 8 2000.0 9 2422.0 10 2195.0 11 1893.0 12 1905.5 13 2044.5 14 2198.0 15 2333.0 16 2407.5 17 2448.0 18 2595.5 19 2869.0 20 3209.0 21 3731.0 22 4459.5 23 5551.5 24 6740.5 25 8480.0 26 11459.0 27 14629.0 28 17226.5 29 20676.0 30 25021.5 31 29388.0 32 34481.0 33 38967.5 34 43485.5 35 48463.0 36 54540.5 37 59942.5 38 61631.0 39 62959.5 40 64263.5 41 66069.0 42 67497.0 43 68885.5 44 71242.5 45 73013.0 46 72566.0 47 70959.5 48 70461.5 49 69583.5 50 67359.5 51 62979.0 52 58992.0 53 57371.5 54 55600.5 55 51236.5 56 45333.5 57 39988.0 58 36123.5 59 31611.0 60 25909.0 61 21213.5 62 17025.0 63 12584.0 64 9053.0 65 6918.0 66 5109.5 67 3677.0 68 2762.5 69 2026.5 70 1482.0 71 1085.0 72 818.0 73 670.0 74 519.5 75 370.0 76 263.0 77 174.5 78 120.0 79 71.5 80 43.0 81 30.0 82 16.0 83 12.5 84 10.0 85 9.0 86 6.0 87 6.5 88 6.5 89 2.5 90 5.0 91 6.5 92 3.5 93 2.5 94 2.0 95 1.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3058639525973982 2 0.2727847871600248 3 0.27467803033291105 4 0.2764135032413901 5 0.2638970622650867 6 0.2022614789700126 7 0.2102551723666434 8 0.20562724461069926 9 0.2046806230242561 10-11 0.21335798756665136 12-13 0.21209582545139385 14-15 0.1971602404208469 16-17 0.2086774697225715 18-19 0.1956088328208429 20-21 0.20202482357340185 22-23 0.19868535297678305 24-25 0.19137533072591675 26-27 0.20241924923441978 28-29 0.20354993612933794 30-31 0.2094400260005396 32-33 0.20988704174969325 34-35 0.2003419407530585 36-37 0.1981857471394936 38-39 0.19021834878693072 40-41 0.1904812992276094 42-43 0.18908766189201257 44-45 0.2055220644344278 46-47 0.1962399138784717 48-49 0.20097302181068724 50-51 0.20967668139715034 52-53 0.21467273977004456 54-55 0.2136998231395336 56-57 0.18532747059030796 58-59 0.17125962201400055 60-61 0.180278822129278 62-63 0.18125173875978898 64-65 0.17914813523435985 66-67 0.1897976280818449 68-69 0.18012105186487082 70-71 0.1842756688275934 72-73 0.19626620892253954 74-75 0.18761513942421215 76-77 0.1975546660818649 78-79 0.1940574252208389 80-81 0.18251390087504646 82-83 0.19489886663101058 84-85 0.17780708798689876 86-87 0.1826190810513179 88-89 0.19016575869879498 90-91 0.18764143446828002 92-93 0.1934263441632102 94-95 0.1918749365632062 96-97 0.18551153589878303 98-99 0.1920852969157491 100-101 0.1766764010919806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1901499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.15208599443727 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41110236304345 44.81358065171283 2 12.37154719240359 14.141194580489778 3 3.881250279071067 6.654646491462099 4 1.6861659816160164 3.8547161272885315 5 0.9099035667698451 2.600144344733769 6 0.5705693501309516 1.9565537138684597 7 0.3758912012670195 1.5038076381555519 8 0.2616939155656677 1.1965082533304034 9 0.20487654026972993 1.0538209482964556 >10 1.1928389265958272 12.771180767070945 >50 0.08947362022915703 3.521136148868515 >100 0.04294053837776891 4.32438728803107 >500 0.001194990556842389 0.4623652947649214 >1k 3.676894021053502E-4 0.4650971428876091 >5k 1.838447010526751E-4 0.6808606090391296 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7668 0.4032607958247677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5292 0.27830674641427633 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3736 0.19647656927508245 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2672 0.1405207154986671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.2590088135728706E-5 0.0 3 0.0 0.0 0.0 5.2590088135728706E-5 0.0 4 0.0 0.0 0.0 5.2590088135728706E-5 0.0 5 0.0 0.0 0.0 5.2590088135728706E-5 0.0 6 0.0 0.0 0.0 5.2590088135728706E-5 0.0 7 0.0 0.0 0.0 5.2590088135728706E-5 0.0 8 0.0 0.0 0.0 5.2590088135728706E-5 0.0 9 0.0 0.0 0.0 5.2590088135728706E-5 0.0 10-11 0.0 0.0 0.0 5.2590088135728706E-5 0.0 12-13 0.0 0.0 0.0 5.2590088135728706E-5 0.0 14-15 0.0 0.0 0.0 5.2590088135728706E-5 0.0 16-17 0.0 0.0 0.0 1.0518017627145741E-4 0.0 18-19 0.0 0.0 0.0 1.5777026440718612E-4 0.0 20-21 0.0 0.0 0.0 1.8406530847505048E-4 0.0 22-23 0.0 0.0 0.0 2.366553966107792E-4 0.0 24-25 0.0 0.0 0.0 3.944256610179653E-4 0.0 26-27 0.0 0.0 0.0 5.259008813572871E-4 0.0 28-29 0.0 0.0 0.0 0.0011569819389860316 0.0 30-31 0.0 2.6295044067864353E-5 0.0 0.0026557994508542996 0.0 32-33 0.0 5.2590088135728706E-5 0.0 0.005443074122047921 0.0 34-35 0.0 5.2590088135728706E-5 0.0 0.009492510908499032 0.0 36-37 0.0 5.2590088135728706E-5 0.0 0.015382600779700646 0.0 38-39 0.0 5.2590088135728706E-5 0.0 0.02597950353904998 0.0 40-41 0.0 5.2590088135728706E-5 0.0 0.03970551654247517 0.0 42-43 0.0 5.2590088135728706E-5 0.0 0.05537736280692233 0.0 44-45 0.0 5.2590088135728706E-5 0.0 0.07599267735612798 0.0 46-47 0.0 5.2590088135728706E-5 0.0 0.10010523276635959 0.0 48-49 0.0 5.2590088135728706E-5 0.0 0.12853017540372097 0.0 50-51 0.0 5.2590088135728706E-5 0.0 0.16150416066482287 0.0 52-53 0.0 7.888513220359306E-5 0.0 0.198501287668308 0.0 54-55 0.0 1.0518017627145741E-4 0.0 0.24088889870570535 0.0 56-57 0.0 1.0518017627145741E-4 0.0 0.29368934719397694 5.2590088135728706E-5 58-59 0.0 1.5777026440718612E-4 0.0 0.3572181736619372 5.2590088135728706E-5 60-61 0.0 1.5777026440718612E-4 0.0 0.4325271798723007 5.2590088135728706E-5 62-63 0.0 1.5777026440718612E-4 0.0 0.5067317942318139 5.2590088135728706E-5 64-65 0.0 1.5777026440718612E-4 0.0 0.592585113113391 5.2590088135728706E-5 66-67 2.6295044067864353E-5 1.5777026440718612E-4 0.0 0.6981334200017986 5.2590088135728706E-5 68-69 5.2590088135728706E-5 1.5777026440718612E-4 0.0 0.8197217037716034 5.2590088135728706E-5 70-71 5.2590088135728706E-5 1.5777026440718612E-4 0.0 0.9580336355685699 5.2590088135728706E-5 72-73 5.2590088135728706E-5 1.5777026440718612E-4 0.0 1.1086779430333646 5.2590088135728706E-5 74-75 5.2590088135728706E-5 1.5777026440718612E-4 0.0 1.2686832861863193 5.2590088135728706E-5 76-77 5.2590088135728706E-5 1.5777026440718612E-4 0.0 1.448488797522376 5.2590088135728706E-5 78-79 5.2590088135728706E-5 1.5777026440718612E-4 0.0 1.654852303366975 5.2590088135728706E-5 80-81 5.2590088135728706E-5 1.5777026440718612E-4 0.0 1.8921124859913152 5.2590088135728706E-5 82-83 5.2590088135728706E-5 1.5777026440718612E-4 0.0 2.146858872920785 5.2590088135728706E-5 84-85 5.2590088135728706E-5 1.5777026440718612E-4 0.0 2.4069168587519636 5.2590088135728706E-5 86-87 5.2590088135728706E-5 1.5777026440718612E-4 0.0 2.688536780718791 7.888513220359306E-5 88-89 5.2590088135728706E-5 1.5777026440718612E-4 0.0 3.0068119941162212 1.0518017627145741E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3635 0.0 48.41065 1 GTATCAA 6840 0.0 43.04058 1 ATCAACG 8345 0.0 34.632214 3 TCAACGC 8470 0.0 34.179035 4 CAACGCA 8595 0.0 33.625763 5 TATCAAC 8695 0.0 33.348377 2 AACGCAG 8855 0.0 32.61694 6 ACGCAGA 10365 0.0 27.681896 7 CGCAGAG 10475 0.0 27.209085 8 GCAGAGT 11440 0.0 24.248905 9 GTACATG 7705 0.0 23.949844 1 TACATGG 7600 0.0 23.767551 2 ACATGGG 7720 0.0 22.843342 3 GAGTACT 6575 0.0 21.7444 12-13 CATGGGG 3560 0.0 21.097597 4 AGAGTAC 10490 0.0 20.716715 10-11 GTACTTT 6965 0.0 20.35635 14-15 CAGAGTA 11145 0.0 19.968012 10-11 AGTACTT 7060 0.0 19.746042 12-13 TGGTATC 1950 0.0 19.74535 2 >>END_MODULE