FastQCFastQC Report
Thu 26 May 2016
SRR937145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901499
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82620.4344993081773906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65340.34362363587885136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.24212476577689496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.045.164031
GTATCAA77900.040.5131721
ATCAACG101200.031.2630163
TCAACGC102750.030.7914074
CAACGCA105200.030.1646215
TATCAAC105400.030.0623112
AACGCAG108250.029.3524256
ACGCAGA124550.025.3979767
CGCAGAG125450.025.1400438
GCAGAGT135150.023.0896829
GAGTACT82900.021.37090512-13
AGAGTAC122350.021.23498510-11
AGTACTT87050.021.00683412-13
GTACATG71000.020.6854881
TACATGG70900.020.3017522
GTACTTT86700.020.07708414-15
TGGTATC25200.019.793652
ACATGGG70700.019.6200713
CAGAGTA133100.018.87757510-11
TAGCCCT11150.018.7462734
Produced by FastQC (version 0.11.5)