##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1901499 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.549303470577687 31.0 31.0 34.0 30.0 34.0 2 31.80247793977278 31.0 31.0 34.0 30.0 34.0 3 31.8708429507457 31.0 31.0 34.0 30.0 34.0 4 35.630640352690165 37.0 35.0 37.0 33.0 37.0 5 35.47100524375769 37.0 35.0 37.0 33.0 37.0 6 35.569642161263296 37.0 35.0 37.0 33.0 37.0 7 35.510813310972026 37.0 35.0 37.0 33.0 37.0 8 35.480155919093306 37.0 35.0 37.0 33.0 37.0 9 37.0631412375184 39.0 37.0 39.0 33.0 39.0 10-11 36.96091005043915 39.0 37.0 39.0 33.0 39.0 12-13 36.85934465387571 39.0 37.0 39.0 33.0 39.0 14-15 37.87547166735297 40.0 37.0 41.0 33.0 41.0 16-17 37.830484265308584 39.0 37.0 41.0 33.0 41.0 18-19 37.81961126458652 39.5 37.0 41.0 33.0 41.0 20-21 37.688261471607404 39.0 37.0 41.0 32.0 41.0 22-23 37.52293190793158 39.0 36.0 40.0 32.0 41.0 24-25 37.35502516698668 39.0 36.0 40.0 32.0 41.0 26-27 37.264853675968276 39.0 36.0 40.0 31.5 41.0 28-29 37.14816074055258 39.0 36.0 40.0 31.0 41.0 30-31 37.23317209212311 39.0 36.0 40.0 31.5 41.0 32-33 37.14154885172172 39.0 36.0 40.0 31.0 41.0 34-35 36.99810465322359 39.0 36.0 40.0 31.0 41.0 36-37 36.786305172918844 39.0 35.0 40.0 30.5 41.0 38-39 36.62533427574771 39.0 35.0 40.0 30.0 41.0 40-41 36.42155425798278 38.5 35.0 40.0 30.0 41.0 42-43 36.18225331698834 38.0 35.0 40.0 30.0 41.0 44-45 35.852738287004094 38.0 35.0 40.0 29.5 41.0 46-47 35.48825400381489 38.0 34.5 40.0 27.5 41.0 48-49 35.16472688126578 38.0 34.0 40.0 27.0 41.0 50-51 34.908015202742675 38.0 34.0 40.0 26.0 41.0 52-53 34.62135557262981 38.0 33.5 40.0 25.5 41.0 54-55 34.33934911351518 38.0 33.0 40.0 24.5 41.0 56-57 34.043500417302354 37.5 33.0 40.0 24.0 41.0 58-59 33.74742242830525 37.0 33.0 40.0 23.0 41.0 60-61 33.35402674416342 36.5 32.0 39.0 22.5 40.0 62-63 32.913686780797676 36.0 31.5 39.0 22.0 40.0 64-65 32.49699579121525 36.0 31.0 39.0 20.5 40.0 66-67 31.9889095392635 35.0 30.5 38.0 18.5 40.0 68-69 31.529424943163264 35.0 30.0 38.0 18.0 40.0 70-71 31.077349238679588 34.0 29.5 37.5 16.0 39.5 72-73 30.552511728904406 34.0 29.0 37.0 13.0 39.0 74-75 30.037656869659145 34.0 29.0 36.0 9.0 39.0 76-77 28.918651022167246 32.5 27.0 35.0 8.0 37.0 78-79 29.58587751032212 34.0 29.0 35.0 7.5 37.5 80-81 29.420565038424947 34.0 29.0 35.0 7.0 37.0 82-83 29.00483539565364 33.5 29.0 35.0 4.0 36.5 84-85 28.51223192859949 33.0 27.5 35.0 2.0 36.0 86-87 27.99219300141625 33.0 26.5 35.0 2.0 36.0 88-89 27.427028886157707 32.0 26.0 34.5 2.0 35.0 90-91 26.774427701513385 32.0 25.0 34.0 2.0 35.0 92-93 26.166261985938462 32.0 24.5 34.0 2.0 35.0 94-95 25.474624230672745 31.0 22.0 34.0 2.0 35.0 96-97 24.667224647501786 31.0 19.0 34.0 2.0 35.0 98-99 23.768696696658793 31.0 7.5 34.0 2.0 35.0 100-101 21.553007390485085 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16205342224203179 1101 2 0.192354979551574 1101 3 0.18137986999131783 1101 4 0.17403213457005506 1101 5 0.04363211520383459 1101 6 0.18055607350696334 1101 7 0.09191902681664033 1101 8 0.1861765056621536 1101 9 0.25100372000459004 1101 10-11 0.19919202837346717 1101 12-13 0.18648360475705772 1101 14-15 0.1774290093165618 1101 16-17 0.10288734327820492 1101 18-19 0.11491444348401103 1101 20-21 0.19814137431350076 1101 22-23 0.16566995835178489 1101 24-25 0.229049691024251 1101 26-27 0.08344318620002866 1101 28-29 0.06893528370878244 1101 30-31 0.2472902486346058 1101 32-33 0.23319154502426898 1101 34-35 0.07324446179453759 1101 36-37 -0.030980888849015287 1101 38-39 -0.23587056677266816 1101 40-41 -0.11938575348889202 1101 42-43 0.24345559046684428 1101 44-45 0.06325863214326688 1101 46-47 -0.08295486671431007 1101 48-49 0.15052887708886686 1101 50-51 -0.03229302754510144 1101 52-53 -0.0011518991367154285 1101 54-55 -0.08713806647713085 1101 56-57 0.04466632079357424 1101 58-59 -0.1505837781225452 1101 60-61 -0.07822787979710455 1101 62-63 -0.23044962428741655 1101 64-65 -0.17258413388547922 1101 66-67 -0.03628259517054744 1101 68-69 -0.10271183450372945 1101 70-71 -0.3981323851293226 1101 72-73 -0.40560261927534924 1101 74-75 -0.1074537481929454 1101 76-77 -0.1392466641127541 1101 78-79 -0.11063695205802304 1101 80-81 -0.13792681967926868 1101 82-83 -0.1218032624703298 1101 84-85 -0.06999650457099804 1101 86-87 -0.1389022578138679 1101 88-89 -0.09238811495834653 1101 90-91 0.08356454540843217 1101 92-93 0.09017180827411764 1101 94-95 0.11355379057937753 1101 96-97 0.3480092515161921 1101 98-99 0.43111894988280497 1101 100-101 0.8762280553307313 1102 1 0.1714905874851027 1102 2 0.20735504404292726 1102 3 0.1565140848174238 1102 4 0.11157850073198716 1102 5 0.008546739054899888 1102 6 0.15050143892953116 1102 7 0.08692136306789422 1102 8 0.033588925365215516 1102 9 0.13009699655224694 1102 10-11 0.020070417238017058 1102 12-13 0.17740374241073198 1102 14-15 0.14154664487218582 1102 16-17 0.1718148687283616 1102 18-19 0.15656223082731202 1102 20-21 0.10669669312337504 1102 22-23 0.20368521825401587 1102 24-25 0.22185859316090983 1102 26-27 0.10925793042108722 1102 28-29 -0.004379005952259263 1102 30-31 0.16288864725191132 1102 32-33 0.12904487587780267 1102 34-35 0.19417583758647794 1102 36-37 0.042205458290048625 1102 38-39 0.044779468614365214 1102 40-41 0.2157528176240575 1102 42-43 0.15619504581865584 1102 44-45 0.10834692912636257 1102 46-47 0.07929029362575335 1102 48-49 0.14137224922934166 1102 50-51 0.08302208760525076 1102 52-53 0.3178890062719475 1102 54-55 0.19445791142604918 1102 56-57 0.11133748105125818 1102 58-59 0.08583538321526163 1102 60-61 0.09823658359531606 1102 62-63 -0.10998757911268342 1102 64-65 0.066065388067166 1102 66-67 0.015439677604575763 1102 68-69 0.18739398540396124 1102 70-71 0.1429614006919806 1102 72-73 -0.06473003879325745 1102 74-75 -0.04451882163195009 1102 76-77 0.0352666238103474 1102 78-79 0.17348563060844668 1102 80-81 0.24845439577885386 1102 82-83 0.26177705193957834 1102 84-85 0.16625362701216773 1102 86-87 0.1998895403839498 1102 88-89 0.2697231322087106 1102 90-91 0.29621250625427464 1102 92-93 0.4273098193524838 1102 94-95 0.3978839937937124 1102 96-97 0.27028416941454125 1102 98-99 0.36563682293303756 1102 100-101 0.7152865564768085 1103 1 0.1100254921762982 1103 2 0.14675496500466423 1103 3 0.13307503950827027 1103 4 0.15303912617830662 1103 5 0.02567577411517874 1103 6 -0.026628281292154554 1103 7 0.010533615548240505 1103 8 -0.04940614351185246 1103 9 0.050547310727296235 1103 10-11 -0.002337215460009645 1103 12-13 0.05492726730513198 1103 14-15 0.04329286842975222 1103 16-17 -0.002882324597678121 1103 18-19 0.05400835287495198 1103 20-21 0.14102429896960444 1103 22-23 0.09142162442748258 1103 24-25 0.055405053833261775 1103 26-27 0.18809910633749638 1103 28-29 0.007699614687325607 1103 30-31 -0.001539179762779952 1103 32-33 0.06601573653433235 1103 34-35 -0.03271158835593724 1103 36-37 -0.15376589462231038 1103 38-39 0.06674378557165994 1103 40-41 -0.008633769199555275 1103 42-43 0.058002954294146036 1103 44-45 -0.22643170052636918 1103 46-47 -0.1320438665233823 1103 48-49 -0.010341755428932231 1103 50-51 -0.1808851594855625 1103 52-53 0.09758524730519724 1103 54-55 -0.0506344161120964 1103 56-57 -0.15563780052764997 1103 58-59 -0.09033684433826039 1103 60-61 -0.37928298530765403 1103 62-63 -0.2711968808312548 1103 64-65 -0.24576645211730153 1103 66-67 -0.10266196037979469 1103 68-69 -0.04558566734155889 1103 70-71 -0.17448274743828307 1103 72-73 -0.0034260379808550567 1103 74-75 -0.05462801107377757 1103 76-77 -0.09037586794222818 1103 78-79 -0.13759192028211942 1103 80-81 -0.11677925037688297 1103 82-83 -0.11689595355761995 1103 84-85 -0.04101860678121838 1103 86-87 0.018456659896081362 1103 88-89 -0.09582709522000954 1103 90-91 0.015178161315475336 1103 92-93 0.14832535090110355 1103 94-95 0.20186716737160637 1103 96-97 0.21082735150801213 1103 98-99 0.41227388355799377 1103 100-101 0.62983296127587 1104 1 0.11044995214326292 1104 2 0.1391306238193799 1104 3 0.043434714804782004 1104 4 0.06228815918971975 1104 5 -2.0453991808722094E-4 1104 6 -0.0778142945812732 1104 7 -0.03818742617382043 1104 8 0.021079376615183776 1104 9 0.09152363575961431 1104 10-11 0.0138316894081143 1104 12-13 0.08820543202747899 1104 14-15 0.12675985911763377 1104 16-17 0.08173431507188411 1104 18-19 -0.06969257732990286 1104 20-21 -0.1516081329595167 1104 22-23 0.03732709568289749 1104 24-25 0.0461812283752252 1104 26-27 -0.015339480764460234 1104 28-29 -0.027077371865004807 1104 30-31 0.10386026978142837 1104 32-33 0.11971264863001352 1104 34-35 0.17984764520022622 1104 36-37 7.588750557374624E-5 1104 38-39 -0.10856279448056938 1104 40-41 0.1658710764387905 1104 42-43 -0.005480863798759117 1104 44-45 0.09248998660972774 1104 46-47 0.07177723786104906 1104 48-49 0.028701221562144497 1104 50-51 -0.18232379356332729 1104 52-53 -0.08590372134935365 1104 54-55 0.031311103696175735 1104 56-57 0.0025416940294533674 1104 58-59 0.20146868756628322 1104 60-61 0.09200661802359633 1104 62-63 0.063874836964942 1104 64-65 -0.11258291009114885 1104 66-67 0.11198308287569247 1104 68-69 0.22510843375541256 1104 70-71 -0.08179738384134794 1104 72-73 -0.008991203071452958 1104 74-75 0.14413354728149486 1104 76-77 0.18981258892769048 1104 78-79 0.10806386617257147 1104 80-81 0.15681099308362434 1104 82-83 0.19696178356744198 1104 84-85 0.13196015428221486 1104 86-87 0.11612984753171673 1104 88-89 0.21436149586649478 1104 90-91 0.2518961714203094 1104 92-93 0.1685528396332039 1104 94-95 0.14442057928316032 1104 96-97 0.43961924281270726 1104 98-99 0.6354945280828375 1104 100-101 0.8428432884036461 1105 1 0.05865625769187588 1105 2 0.10460967730516302 1105 3 0.10473537627365204 1105 4 0.10027102009940592 1105 5 -0.012120981170099299 1105 6 0.04160424927797379 1105 7 0.10858546951412507 1105 8 0.019740527252785967 1105 9 0.10613686968319058 1105 10-11 0.0035173212131738296 1105 12-13 -0.020160217053060592 1105 14-15 0.03916365062992355 1105 16-17 0.153581199803547 1105 18-19 0.12215021584173513 1105 20-21 0.12801953507857888 1105 22-23 0.11996501535193715 1105 24-25 0.18941901620510748 1105 26-27 0.14102835048142737 1105 28-29 0.17031448593055387 1105 30-31 0.1676790591342865 1105 32-33 0.11192186373241242 1105 34-35 0.14499164538207054 1105 36-37 0.15516124199378112 1105 38-39 0.1679391490104365 1105 40-41 0.1314165041204589 1105 42-43 0.13986021319076514 1105 44-45 0.3071229964067399 1105 46-47 0.31983556532224355 1105 48-49 0.2069832209711393 1105 50-51 0.3302122917229653 1105 52-53 0.3078677877543683 1105 54-55 0.20030958072661775 1105 56-57 0.13925276169930356 1105 58-59 0.3455227416203357 1105 60-61 0.2943540369311535 1105 62-63 0.254419241756608 1105 64-65 0.33817221004159137 1105 66-67 0.3843089074386903 1105 68-69 0.4111950839042997 1105 70-71 0.4213406153858017 1105 72-73 0.549525379112076 1105 74-75 0.5523634929546724 1105 76-77 0.36399461788684206 1105 78-79 0.6163522302304898 1105 80-81 0.22379483142153944 1105 82-83 0.486625593719058 1105 84-85 0.3783429879148379 1105 86-87 0.5341199823258869 1105 88-89 0.5240209871491999 1105 90-91 0.6089102418406007 1105 92-93 0.5974024293406579 1105 94-95 0.5310955048394987 1105 96-97 0.6238044540963159 1105 98-99 0.6564164929408811 1105 100-101 0.8281427678662858 1106 1 -0.09587983118046495 1106 2 0.019776312940983587 1106 3 0.04115317556614784 1106 4 0.1408109567964857 1106 5 0.04923568735841144 1106 6 -0.10968849571054307 1106 7 -0.05511340716929425 1106 8 0.008463918303483808 1106 9 0.011276818792609333 1106 10-11 -0.2659135730957729 1106 12-13 -0.23770940094184567 1106 14-15 -0.10344356093838059 1106 16-17 -0.04584845327160991 1106 18-19 -0.00735018755924699 1106 20-21 0.03348965237517376 1106 22-23 0.10449365682156042 1106 24-25 0.09914656538899891 1106 26-27 0.1226152678773147 1106 28-29 0.1591675859119306 1106 30-31 0.2104811444070478 1106 32-33 0.1261706069832087 1106 34-35 0.004686922487515233 1106 36-37 0.14361342346216333 1106 38-39 0.27145494371036705 1106 40-41 0.13565283503339032 1106 42-43 -0.22520464127457274 1106 44-45 0.012146890157126222 1106 46-47 0.12531064755398091 1106 48-49 0.035664410073110275 1106 50-51 0.005083513717629273 1106 52-53 0.18406775051735025 1106 54-55 0.0804808709891347 1106 56-57 0.2369359988370192 1106 58-59 0.13720213093331068 1106 60-61 0.4092538073029033 1106 62-63 0.40267096396848956 1106 64-65 0.3097622305219545 1106 66-67 0.27917454388132157 1106 68-69 0.3033312472506644 1106 70-71 0.20083543323474373 1106 72-73 0.1301764062330335 1106 74-75 -0.02854074585870947 1106 76-77 0.08780210252657383 1106 78-79 0.15512651186643822 1106 80-81 0.1808003549118915 1106 82-83 0.11806418367747895 1106 84-85 0.0045659135028728315 1106 86-87 0.12159655403817027 1106 88-89 -0.0025879621464000024 1106 90-91 0.034614952322822745 1106 92-93 0.02025788766622938 1106 94-95 -0.7267826060026792 1106 96-97 -0.22045856334301916 1106 98-99 0.10467442132169325 1106 100-101 0.574572358879756 1107 1 0.057656104294114385 1107 2 0.0916147630309112 1107 3 -0.022604824559415704 1107 4 0.041184326765133505 1107 5 0.009401432602658133 1107 6 0.13297243930239944 1107 7 0.04653111950763389 1107 8 -0.017592947678480186 1107 9 -0.004516250781250619 1107 10-11 0.08144975539963184 1107 12-13 0.04500515753247214 1107 14-15 -0.05713792936700912 1107 16-17 -0.0911329493698787 1107 18-19 0.08407264377428447 1107 20-21 0.24865342182383188 1107 22-23 0.13621090016201975 1107 24-25 0.062218761328828975 1107 26-27 0.05004448084591928 1107 28-29 0.053033756229211804 1107 30-31 0.08702051074909178 1107 32-33 0.004132915451059205 1107 34-35 0.06581397537605227 1107 36-37 -0.11477669091239306 1107 38-39 -0.10616907900943318 1107 40-41 0.03593277018297414 1107 42-43 0.09122474526859747 1107 44-45 0.14109765037679267 1107 46-47 -0.08135055336042285 1107 48-49 -0.11946656486198748 1107 50-51 0.07282264961174434 1107 52-53 -0.08334971847003914 1107 54-55 -0.17085990019896258 1107 56-57 0.11143974176939508 1107 58-59 0.1546519143344156 1107 60-61 0.29423037473662106 1107 62-63 0.09091516491613305 1107 64-65 0.017836032949780645 1107 66-67 0.1517582566781961 1107 68-69 0.22788133828498403 1107 70-71 -0.0041753320813633366 1107 72-73 0.22383798014809742 1107 74-75 0.26348848852973816 1107 76-77 0.28215797470183546 1107 78-79 0.2323472507031923 1107 80-81 0.34624128970325074 1107 82-83 0.29129159959537176 1107 84-85 0.3362502884682499 1107 86-87 0.39832980686244923 1107 88-89 0.37528238873273523 1107 90-91 0.33658225382922424 1107 92-93 0.3859935299105892 1107 94-95 0.38955999325796853 1107 96-97 0.2062889078232928 1107 98-99 0.5153592283769477 1107 100-101 1.1536159999571005 1108 1 -0.03449671866547632 1108 2 -0.003253139254038473 1108 3 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-0.13764279270976232 2316 70-71 -0.2195489111565223 2316 72-73 -0.055610919309732054 2316 74-75 -0.266201786815774 2316 76-77 -0.0863784093728448 2316 78-79 -0.4389131930119774 2316 80-81 -0.46025107962580236 2316 82-83 -0.5025709629523902 2316 84-85 -0.5176111010378186 2316 86-87 -0.5857652400557498 2316 88-89 -0.5794940774132087 2316 90-91 -0.5510323693502137 2316 92-93 -0.6056391775459993 2316 94-95 -0.45844538468704243 2316 96-97 -0.386869864411576 2316 98-99 -0.6114170795124885 2316 100-101 -1.1114124688625502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 28.0 5 51.0 6 137.0 7 317.0 8 747.0 9 1369.0 10 2421.0 11 3652.0 12 5226.0 13 7340.0 14 9378.0 15 12829.0 16 16544.0 17 20603.0 18 18429.0 19 13666.0 20 12988.0 21 13753.0 22 14948.0 23 17071.0 24 20310.0 25 24338.0 26 29849.0 27 36857.0 28 45394.0 29 55558.0 30 68273.0 31 82804.0 32 103235.0 33 128650.0 34 165997.0 35 219767.0 36 288946.0 37 313574.0 38 140992.0 39 5446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.593009159482754 21.740512190193968 16.05271964237608 21.6137590079472 2 16.32091313221832 22.367616285888133 38.604280096913016 22.707190484980533 3 19.792542620322177 25.87842538965311 30.44077330569198 23.888258684332726 4 13.855226849974677 18.633614848075126 37.18702981174326 30.324128490206938 5 13.21320689515516 38.27055168289398 34.625311444920335 13.890929977030526 6 30.920868220283047 38.25718551521721 17.13621726858652 13.685728995913223 7 27.602118118389757 33.38723817367246 21.133747638047666 17.876896069890126 8 26.06149148645358 35.94737625420786 19.597801523955575 18.39333073538298 9 25.846135075537774 17.029564569847263 20.64602716067692 36.478273193938044 10-11 24.46448828003591 27.69217864432219 27.49275703011151 20.350576045530396 12-13 25.45730937685385 24.797680504541802 28.251706068217487 21.493304050386865 14-15 22.344108516491463 26.259808708813416 26.55533870909214 24.84074406560298 16-17 21.382840590502543 29.59778048792032 27.256601239338018 21.76277768223912 18-19 21.308478205878625 27.840535282953084 30.099700289087718 20.75128622208058 20-21 22.010582177534673 26.908034135174407 30.37109143891214 20.710292248378778 22-23 21.819858021381727 26.815720462470487 30.24422773061139 21.120193785536397 24-25 21.608474796436703 27.11074484997873 30.28866203838344 20.99211831520113 26-27 21.435686183559024 27.304006718973262 30.151865835104317 21.10844126236339 28-29 21.363222322497315 27.334419880553433 30.28153458754331 21.020823209405943 30-31 21.479133041879063 27.09909392537151 30.306405630505196 21.115367402244228 32-33 21.33143377934987 27.223495778856577 30.126705299345414 21.318365142448144 34-35 21.80066884074091 27.186577536985297 29.961703897819564 21.05104972445423 36-37 21.816235506829084 26.943216904137206 30.01121746579935 21.22933012323435 38-39 21.52699002208258 27.3359859773789 29.91371544239571 21.22330855814281 40-41 21.780421656808656 27.227361150334552 29.894414880049897 21.097802312806895 42-43 21.589183238574726 27.214580842079183 29.72392411351677 21.472311805829317 44-45 21.776503695242543 27.339325447975522 29.650607231452657 21.23356362532928 46-47 21.941321031459918 27.670511528010273 29.009271106637446 21.378896333892367 48-49 21.911502451486957 27.773272560227486 28.811322014894564 21.503902973390993 50-51 22.031749688009302 27.81942036256659 28.735618583023182 21.413211366400926 52-53 22.19761882598939 27.86617295091925 28.49273126096832 21.44347696212304 54-55 22.034563257724564 27.937905831136383 28.639089476250053 21.388441434889 56-57 22.0742161841789 28.12888673620128 28.414661275130833 21.382235804488985 58-59 22.072217760829744 27.78786630968515 28.551526979504064 21.58838894998104 60-61 21.919785390368336 27.94571545922454 28.67445631052133 21.460042839885794 62-63 21.87403201369025 27.877979425705718 28.90732522078634 21.34066333981769 64-65 21.8355360691749 27.774613607474947 28.782108220935164 21.60774210241499 66-67 21.7872583682663 27.92744040359737 28.526888523212474 21.758412704923852 68-69 21.915394118009 27.83966754649884 28.622076582738142 21.622861752754012 70-71 21.982867201087142 27.754024587969806 28.623812055646624 21.639296155296428 72-73 21.958418584079872 27.754321131465527 28.550877110868083 21.736383173586514 74-75 21.83503646333761 28.135986398099604 28.204327217631985 21.824649920930803 76-77 21.9109765506056 27.826178188892026 28.49646515722596 21.766380103276415 78-79 21.985917426199013 27.88968072031592 28.292126369774586 21.832275483710482 80-81 21.910687305120856 28.043454138024792 28.259941235835516 21.78591732101884 82-83 21.993992626334048 27.995476194670882 28.19032272738867 21.82020845160641 84-85 21.91586559763383 27.990272786389685 28.19611358478272 21.89774803119377 86-87 21.854521090991895 28.016449127767096 28.198831553421797 21.930198227819208 88-89 21.869535561154645 27.90545774675664 28.150685327733544 22.074321364355175 90-91 21.859000269026243 27.97041921020943 28.13775195569982 22.03282856506451 92-93 21.95575385759435 27.845624176791034 28.192854342401812 22.005767623212805 94-95 21.743371939717036 27.81305696190216 28.18137164416074 22.262199454220067 96-97 21.882709378232647 27.70298590743408 28.136959314730113 22.277345399603156 98-99 21.628295593341566 27.72094099518932 28.14258235942987 22.508181052039248 100-101 21.75446126139773 27.59176039418153 28.270311835965277 22.383466508455463 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 439.0 1 294.5 2 224.0 3 315.5 4 277.0 5 266.0 6 500.0 7 1113.0 8 1844.5 9 2174.0 10 2076.5 11 1907.5 12 1931.0 13 2112.5 14 2335.0 15 2499.0 16 2594.0 17 2740.0 18 2973.0 19 3201.0 20 3576.5 21 4199.0 22 4948.0 23 5980.0 24 7197.0 25 8924.0 26 11797.5 27 15083.5 28 17991.0 29 21588.0 30 25991.5 31 30333.0 32 35108.5 33 39778.5 34 44573.0 35 49104.5 36 54814.5 37 60272.0 38 62443.5 39 63655.0 40 64724.5 41 66645.5 42 67981.0 43 69731.0 44 72167.0 45 73019.0 46 71958.5 47 70803.5 48 70307.5 49 68903.0 50 66514.5 51 62366.5 52 58378.0 53 56553.0 54 54931.0 55 50438.5 56 44617.5 57 39236.0 58 34829.5 59 30643.5 60 25280.5 61 20439.0 62 16378.0 63 12190.5 64 8805.0 65 6557.5 66 4805.0 67 3554.0 68 2580.5 69 1877.5 70 1423.0 71 1034.0 72 748.0 73 613.5 74 500.0 75 351.5 76 258.0 77 151.0 78 87.5 79 65.0 80 36.5 81 22.5 82 14.0 83 6.5 84 6.0 85 6.0 86 2.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050223534169620915 2 0.0 3 0.0 4 0.0 5 0.014725224678004039 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002682094494922164 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.15146366103795E-4 24-25 1.0518017627145742E-4 26-27 9.466215864431166E-4 28-29 0.0033131755525509085 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013910078311900241 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.6295044067864356E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3147522033932178E-4 84-85 0.0015514076000039969 86-87 0.0 88-89 0.0 90-91 0.01038654240680642 92-93 0.001183276983053896 94-95 0.0 96-97 0.0 98-99 7.888513220359306E-5 100-101 0.012779391416982075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1901499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.3665535877058 #Duplication Level Percentage of deduplicated Percentage of total 1 78.31081014629902 44.92421286754318 2 12.541413569252596 14.389153471722196 3 3.8036021096349355 6.5459863274605015 4 1.7150335398216827 3.9354225386757333 5 0.9216591239924222 2.643620376305464 6 0.5666728558366836 1.9504841250632035 7 0.378381094050054 1.5194493515878018 8 0.2692438373258806 1.2356472817691737 9 0.1898350937142901 0.9801166568749178 >10 1.1703396602063592 12.474444414243568 >50 0.09001751965446574 3.5224963039062085 >100 0.041067080989842256 4.141463092825435 >500 0.0013745494441733076 0.5049756644853558 >1k 3.6654651844621525E-4 0.4547199144074877 >5k 1.8327325922310762E-4 0.7778076131298483 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8262 0.4344993081773906 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6534 0.34362363587885136 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4604 0.24212476577689496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.2590088135728706E-5 0.0 3 0.0 0.0 0.0 5.2590088135728706E-5 0.0 4 0.0 0.0 0.0 5.2590088135728706E-5 0.0 5 0.0 0.0 0.0 5.2590088135728706E-5 0.0 6 0.0 0.0 0.0 5.2590088135728706E-5 0.0 7 0.0 0.0 0.0 1.0518017627145741E-4 0.0 8 0.0 0.0 0.0 1.0518017627145741E-4 0.0 9 0.0 0.0 0.0 1.5777026440718612E-4 0.0 10-11 0.0 0.0 0.0 2.6295044067864355E-4 0.0 12-13 0.0 0.0 0.0 3.1554052881437223E-4 0.0 14-15 0.0 0.0 0.0 3.1554052881437223E-4 0.0 16-17 0.0 0.0 0.0 3.944256610179653E-4 0.0 18-19 0.0 0.0 0.0 5.259008813572871E-4 0.0 20-21 0.0 0.0 0.0 5.521959254251514E-4 0.0 22-23 0.0 0.0 0.0 7.099661898323376E-4 0.0 24-25 0.0 0.0 0.0 8.414414101716593E-4 0.0 26-27 0.0 0.0 0.0 9.466215864431166E-4 0.0 28-29 0.0 0.0 0.0 0.00163029273220759 0.0 30-31 0.0 0.0 0.0 0.0031028152000079938 0.0 32-33 0.0 0.0 0.0 0.005916384915269479 0.0 34-35 0.0 0.0 0.0 0.009992116745788454 0.0 36-37 0.0 0.0 0.0 0.015855911572922203 0.0 38-39 0.0 0.0 0.0 0.026137273803457168 0.0 40-41 0.0 0.0 0.0 0.03981069671874663 0.0 42-43 0.0 0.0 0.0 0.05521959254251514 0.0 44-45 0.0 0.0 0.0 0.07583490709172079 0.0 46-47 0.0 0.0 0.0 0.09944785666466298 0.0 48-49 0.0 0.0 0.0 0.1276887339935493 0.0 50-51 0.0 5.2590088135728706E-5 0.0 0.16032088368176897 0.0 52-53 0.0 5.2590088135728706E-5 0.0 0.19700247015643974 0.0 54-55 0.0 1.0518017627145741E-4 0.0 0.23910083570909058 0.0 56-57 0.0 1.0518017627145741E-4 0.0 0.2904813518176975 0.0 58-59 0.0 1.0518017627145741E-4 0.0 0.35282690130260386 0.0 60-61 0.0 1.5777026440718612E-4 0.0 0.4266370900010991 0.0 62-63 0.0 1.5777026440718612E-4 0.0 0.499158821540269 0.0 64-65 0.0 1.5777026440718612E-4 0.0 0.5826981765438741 0.0 66-67 0.0 1.8406530847505048E-4 0.0 0.6858010443339702 0.0 68-69 0.0 2.1036035254291482E-4 0.0 0.8056538551952959 0.0 70-71 0.0 2.1036035254291482E-4 0.0 0.9398637601176756 0.0 72-73 0.0 2.1036035254291482E-4 0.0 1.0872474821180553 0.0 74-75 0.0 2.1036035254291482E-4 0.0 1.2448599762608343 0.0 76-77 0.0 2.1036035254291482E-4 0.0 1.4222200484985792 0.0 78-79 0.0 2.1036035254291482E-4 0.0 1.6262432954211388 0.0 80-81 0.0 2.1036035254291482E-4 0.0 1.8604006628454708 0.0 82-83 0.0 2.1036035254291482E-4 0.0 2.109072894595264 0.0 84-85 0.0 2.1036035254291482E-4 0.0 2.3638718716128695 0.0 86-87 0.0 2.1036035254291482E-4 0.0 2.6375769853152695 0.0 88-89 0.0 2.1036035254291482E-4 0.0 2.9468592936414906 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4420 0.0 45.16403 1 GTATCAA 7790 0.0 40.513172 1 ATCAACG 10120 0.0 31.263016 3 TCAACGC 10275 0.0 30.791407 4 CAACGCA 10520 0.0 30.164621 5 TATCAAC 10540 0.0 30.062311 2 AACGCAG 10825 0.0 29.352425 6 ACGCAGA 12455 0.0 25.397976 7 CGCAGAG 12545 0.0 25.140043 8 GCAGAGT 13515 0.0 23.089682 9 GAGTACT 8290 0.0 21.370905 12-13 AGAGTAC 12235 0.0 21.234985 10-11 AGTACTT 8705 0.0 21.006834 12-13 GTACATG 7100 0.0 20.685488 1 TACATGG 7090 0.0 20.301752 2 GTACTTT 8670 0.0 20.077084 14-15 TGGTATC 2520 0.0 19.79365 2 ACATGGG 7070 0.0 19.620071 3 CAGAGTA 13310 0.0 18.877575 10-11 TAGCCCT 1115 0.0 18.746273 4 >>END_MODULE