FastQCFastQC Report
Thu 26 May 2016
SRR937144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974223
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79410.40223419542777084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.28345328769850214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38900.19703954416497022No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28190.1427903534707072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.047.377781
GTATCAA67450.042.6889151
ATCAACG84050.033.3456733
TCAACGC85650.032.7277374
CAACGCA87150.032.164445
TATCAAC88800.031.8294562
AACGCAG90250.031.1083156
ACGCAGA103350.026.8894317
CGCAGAG103550.026.74718
GTACATG76100.025.2868561
TACATGG74000.025.1639922
ACATGGG74400.024.2625163
GCAGAGT114550.023.7224339
CATGGGG34100.022.4317344
GAGTACT66300.021.45895212-13
AGAGTAC104650.021.13110510-11
AGTACTT72550.020.788912-13
TGGTATC19700.020.7375472
GTACTTT72200.019.3739514-15
TCGTATA1250.006030104619.001382
Produced by FastQC (version 0.11.5)