FastQCFastQC Report
Thu 26 May 2016
SRR937144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974223
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84860.42983999274651347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67590.34236253959152535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.24328558627875368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.049.2522241
GTATCAA77450.042.4082451
ATCAACG100850.032.262673
TCAACGC102750.031.6660844
TATCAAC103350.031.5741652
CAACGCA104600.031.1060245
AACGCAG109000.029.8496116
ACGCAGA124150.026.0560177
CGCAGAG124200.025.8542968
GCAGAGT135150.023.4080819
CCGTGCG1050.00219636422.6197789
GAGTACT81650.022.10726512-13
GTACATG78600.021.2869681
AGAGTAC123800.021.00734110-11
GTACTTT85650.020.82366614-15
AGTACTT86400.020.7544312-13
TACATGG80100.020.3991262
ACATGGG75250.020.1358413
TGGTATC22500.020.0551872
CAGAGTA133900.019.15670410-11
Produced by FastQC (version 0.11.5)