##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937144_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1974223 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613910383983978 31.0 31.0 34.0 30.0 34.0 2 31.861303915515116 31.0 31.0 34.0 30.0 34.0 3 31.89412847484808 31.0 31.0 34.0 30.0 34.0 4 35.68167324562626 37.0 35.0 37.0 33.0 37.0 5 35.56849707454528 37.0 35.0 37.0 33.0 37.0 6 35.62647380767016 37.0 35.0 37.0 33.0 37.0 7 35.552123037772326 37.0 35.0 37.0 33.0 37.0 8 35.51878131295198 37.0 35.0 37.0 33.0 37.0 9 37.12258088371982 39.0 37.0 39.0 33.0 39.0 10-11 37.00416366337541 39.0 37.0 39.0 33.0 39.0 12-13 36.89003623197582 39.0 37.0 39.0 33.0 39.0 14-15 37.8931004754782 40.0 37.0 41.0 33.0 41.0 16-17 37.8305477648675 39.0 37.0 41.0 33.0 41.0 18-19 37.8054057216434 39.0 37.0 41.0 33.0 41.0 20-21 37.6844128044299 39.0 37.0 41.0 32.0 41.0 22-23 37.49337663475707 39.0 36.0 40.0 32.0 41.0 24-25 37.421150498196 39.0 36.0 40.0 32.0 41.0 26-27 37.33514425675317 39.0 36.0 40.0 32.0 41.0 28-29 37.17750856919406 39.0 36.0 40.0 31.0 41.0 30-31 37.239557790583945 39.0 36.0 40.0 31.5 41.0 32-33 37.164088605998415 39.0 36.0 40.0 31.0 41.0 34-35 36.99934252614826 39.0 36.0 40.0 31.0 41.0 36-37 36.77425473211487 39.0 35.0 40.0 30.5 41.0 38-39 36.619882100451676 39.0 35.0 40.0 30.0 41.0 40-41 36.397767121546046 38.0 35.0 40.0 30.0 41.0 42-43 36.1510163745433 38.0 35.0 40.0 30.0 41.0 44-45 35.81716731088636 38.0 35.0 40.0 29.0 41.0 46-47 35.451712141941414 38.0 34.5 40.0 27.5 41.0 48-49 35.09490163978436 38.0 34.0 40.0 26.5 41.0 50-51 34.801611317465145 38.0 34.0 40.0 26.0 41.0 52-53 34.49554432300708 38.0 33.0 40.0 24.5 41.0 54-55 34.21207178722971 38.0 33.0 40.0 24.0 41.0 56-57 33.872416135360595 37.0 33.0 40.0 23.5 41.0 58-59 33.53771078545838 37.0 32.0 39.5 23.0 40.5 60-61 33.13948576224672 36.5 32.0 39.0 22.0 40.0 62-63 32.69987787600489 36.0 31.0 39.0 20.5 40.0 64-65 32.21858498254757 35.5 31.0 39.0 19.5 40.0 66-67 31.710214094355095 35.0 30.0 38.0 18.0 40.0 68-69 31.181118597037923 34.5 29.5 38.0 15.0 40.0 70-71 30.71784418477548 34.0 29.0 37.0 12.5 39.0 72-73 30.14271234809847 34.0 28.5 36.5 8.0 39.0 74-75 29.578961697842644 34.0 28.0 36.0 7.5 39.0 76-77 28.54931509763588 31.5 27.0 34.5 7.0 37.0 78-79 29.155320093018872 33.0 28.0 35.0 7.0 37.0 80-81 28.922848128099005 33.0 28.0 35.0 2.0 37.0 82-83 28.477271564559828 33.0 27.0 35.0 2.0 36.5 84-85 27.979211821562203 33.0 26.5 35.0 2.0 36.0 86-87 27.397133454528692 32.0 26.0 35.0 2.0 35.5 88-89 26.70694318726912 32.0 25.0 34.0 2.0 35.0 90-91 25.95210115574583 31.0 24.0 34.0 2.0 35.0 92-93 25.315382558100076 31.0 21.5 34.0 2.0 35.0 94-95 24.561847116561808 31.0 19.0 34.0 2.0 35.0 96-97 23.740756490021646 31.0 8.5 34.0 2.0 35.0 98-99 22.541887618571963 30.0 2.0 34.0 2.0 35.0 100-101 19.84483389161204 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1537399024622026 1101 2 -0.0659869624273739 1101 3 -0.06381034721761836 1101 4 -0.032904614063205884 1101 5 -0.04318119340464932 1101 6 -0.18779785182655928 1101 7 -0.13671440339641094 1101 8 -0.1272565014297129 1101 9 -0.1759674939104343 1101 10-11 -0.16384126631397322 1101 12-13 -0.18527637059662538 1101 14-15 -0.3301607097994719 1101 16-17 -0.17003561655971566 1101 18-19 -0.10785491426628369 1101 20-21 -0.21642029569431287 1101 22-23 -0.1711244501843865 1101 24-25 -0.1218431744104791 1101 26-27 -0.21277552564344404 1101 28-29 -0.41508069313091056 1101 30-31 -0.3400789198455598 1101 32-33 -0.23942246169688275 1101 34-35 -0.23704270510732073 1101 36-37 -0.2850599810407033 1101 38-39 -0.3743146201986036 1101 40-41 -0.29189772100217937 1101 42-43 -0.253334292449118 1101 44-45 -0.22921718853267237 1101 46-47 -0.0866525400691387 1101 48-49 -0.13634192161855907 1101 50-51 -0.040772975121925015 1101 52-53 -0.16459183192380777 1101 54-55 -0.19080043167007688 1101 56-57 -0.1192467709661642 1101 58-59 -0.16509557460444313 1101 60-61 -0.12913538003325442 1101 62-63 -0.2390886655948563 1101 64-65 -0.22817853921465314 1101 66-67 -0.05757898603675571 1101 68-69 -0.006047345338426879 1101 70-71 -0.19163941426716136 1101 72-73 -0.06401290895392364 1101 74-75 -0.015921440087939942 1101 76-77 0.11861686152008843 1101 78-79 -0.07326134984205979 1101 80-81 -0.1199751388234489 1101 82-83 0.034983912537388306 1101 84-85 -0.1612628235056981 1101 86-87 -0.07101923749462813 1101 88-89 -0.09949043572720129 1101 90-91 0.08142750111328212 1101 92-93 0.2939979335746621 1101 94-95 0.36075965639647123 1101 96-97 0.5588503507618476 1101 98-99 0.540795234390238 1101 100-101 1.104610900798594 1102 1 -0.09469911719832425 1102 2 -0.09899724416564126 1102 3 -0.04084883384514626 1102 4 -0.01873383159497166 1102 5 -0.18732743637672655 1102 6 -0.1354541746296576 1102 7 -0.15136665699191099 1102 8 -0.10548779394672181 1102 9 -0.10895603179133673 1102 10-11 0.020748551348255262 1102 12-13 -0.04606908951067368 1102 14-15 -0.10837491785712672 1102 16-17 -0.1247602461375692 1102 18-19 -0.11811801494035024 1102 20-21 -0.03302639018691167 1102 22-23 -0.03259122867660835 1102 24-25 0.024583067319092322 1102 26-27 -0.2204821699450008 1102 28-29 -0.1407058290300185 1102 30-31 -0.0017709025747265628 1102 32-33 -0.01632893204757835 1102 34-35 -0.08967681811325434 1102 36-37 -0.05842530890700459 1102 38-39 0.0800077480180903 1102 40-41 0.12324066311858672 1102 42-43 0.005866465479620331 1102 44-45 0.1817898626896408 1102 46-47 0.27031636621483557 1102 48-49 0.32722003284359147 1102 50-51 0.2871445845960281 1102 52-53 0.22357950967219153 1102 54-55 0.30022090837279336 1102 56-57 0.2665855528309393 1102 58-59 0.2801627113583436 1102 60-61 0.37883529682196126 1102 62-63 0.4779290090549111 1102 64-65 0.2696082467326022 1102 66-67 0.4243114870474578 1102 68-69 0.45164998016711877 1102 70-71 0.5407511675363388 1102 72-73 0.465114541112861 1102 74-75 0.3877299748159224 1102 76-77 0.5581891287831588 1102 78-79 0.6589366435105326 1102 80-81 0.4385189437410837 1102 82-83 0.6198595066106343 1102 84-85 0.4645455820483022 1102 86-87 0.5781653678543606 1102 88-89 0.729470829144045 1102 90-91 0.8978239226956113 1102 92-93 0.9048465652647799 1102 94-95 1.0806180106970942 1102 96-97 1.1693635057955483 1102 98-99 1.291149348638676 1102 100-101 1.4676758528067957 1103 1 -0.12702563972292324 1103 2 0.007285542392288846 1103 3 5.20081085962687E-4 1103 4 -0.07680211761051936 1103 5 -0.08986422758302837 1103 6 -0.11925441786560498 1103 7 -0.07888517517344695 1103 8 -0.12110402706502299 1103 9 -0.10834096632689949 1103 10-11 -0.13601873574194911 1103 12-13 -0.04495956560005254 1103 14-15 -0.022046678729708447 1103 16-17 -0.196529711837357 1103 18-19 -0.0786796210659304 1103 20-21 -0.2742200053949162 1103 22-23 -0.13090495452361495 1103 24-25 -0.057803859810213964 1103 26-27 -0.17266962539976305 1103 28-29 -0.1806381758727511 1103 30-31 -0.17258400415656894 1103 32-33 -0.027092982057531856 1103 34-35 -0.052396713620872504 1103 36-37 -0.19241141635340142 1103 38-39 -0.02061024921795962 1103 40-41 -0.06952746508677166 1103 42-43 -0.034460861765730044 1103 44-45 -0.127498090863007 1103 46-47 0.09010986839799529 1103 48-49 0.03677942800899814 1103 50-51 0.11931279145374418 1103 52-53 0.31737112945578616 1103 54-55 0.024512658064203663 1103 56-57 -0.0928150717375118 1103 58-59 -0.011800594992578795 1103 60-61 0.0561073945449877 1103 62-63 0.31367610028254944 1103 64-65 0.04664246657301163 1103 66-67 0.4374761525272568 1103 68-69 0.4293902514874155 1103 70-71 0.4313900207890349 1103 72-73 0.5906900023866832 1103 74-75 0.8455950587303 1103 76-77 0.6854057755845453 1103 78-79 0.6586547890537879 1103 80-81 0.37479112537343084 1103 82-83 0.5615104781896356 1103 84-85 0.5292141958157011 1103 86-87 0.49202011171356475 1103 88-89 0.7185823304840646 1103 90-91 0.6561092609057972 1103 92-93 0.9489934854387023 1103 94-95 1.014474816905981 1103 96-97 0.9355666492612862 1103 98-99 1.240992397762028 1103 100-101 1.8294016755478388 1104 1 0.019231796912851706 1104 2 0.11121470601927186 1104 3 0.11919322714328473 1104 4 -0.035543920509610416 1104 5 0.028850307065553693 1104 6 -0.08447453987354692 1104 7 0.018672757301672505 1104 8 0.05453709929133055 1104 9 0.04821901488348601 1104 10-11 0.0621042313699931 1104 12-13 0.12189231166388481 1104 14-15 0.21879805438118893 1104 16-17 -0.03738927251153257 1104 18-19 0.1215044376158616 1104 20-21 -0.02996623868151005 1104 22-23 -0.08898514270909885 1104 24-25 0.08209932303272893 1104 26-27 -0.08652650566035902 1104 28-29 0.15150788084931577 1104 30-31 0.014062770537002223 1104 32-33 0.03898269838798285 1104 34-35 0.07240033661825152 1104 36-37 0.14709126154872365 1104 38-39 -0.04745135339324236 1104 40-41 0.01628987018311534 1104 42-43 -0.029597099546812444 1104 44-45 0.08561970167045274 1104 46-47 0.17634510789026336 1104 48-49 0.1941063036505426 1104 50-51 0.013251010341932101 1104 52-53 0.036703539515464456 1104 54-55 0.047110326600652286 1104 56-57 0.0370262632896754 1104 58-59 -0.0767812860092647 1104 60-61 0.0856816526092814 1104 62-63 0.14735253030358564 1104 64-65 0.2410561318803488 1104 66-67 0.4344440363868536 1104 68-69 0.5073056809332286 1104 70-71 0.42750481747159697 1104 72-73 0.3582660393567423 1104 74-75 0.3419872192489173 1104 76-77 0.4810308206649587 1104 78-79 0.1408411192861223 1104 80-81 0.11472229574434678 1104 82-83 0.23343275925553897 1104 84-85 0.18492370175044215 1104 86-87 0.2770086906724458 1104 88-89 0.5836379428875453 1104 90-91 0.6379234956478221 1104 92-93 0.8798388755432534 1104 94-95 0.8821759840441601 1104 96-97 0.9823222960414064 1104 98-99 1.0814409857982739 1104 100-101 1.4965047976339356 1105 1 0.038174483186374175 1105 2 0.007400464216921421 1105 3 -0.022380130093079487 1105 4 0.017506929182225406 1105 5 -0.09846196940992513 1105 6 -0.11929802295509262 1105 7 -0.05086381112474214 1105 8 -0.04188172994890493 1105 9 0.05067721821799154 1105 10-11 0.021105683841710743 1105 12-13 -0.07357326607466064 1105 14-15 -0.10318250573079268 1105 16-17 -0.10634470807590901 1105 18-19 -0.040251005857612654 1105 20-21 0.04406821365096647 1105 22-23 -0.007654965323006024 1105 24-25 -0.17252080165079775 1105 26-27 0.029576281223405942 1105 28-29 -0.09925172148815165 1105 30-31 -0.2508174437993844 1105 32-33 -0.10076744846572439 1105 34-35 -0.11408059546818805 1105 36-37 -0.08649078250655862 1105 38-39 -0.21778084944405407 1105 40-41 -0.185080544706409 1105 42-43 -0.18830002500444465 1105 44-45 -0.2099466551959992 1105 46-47 -0.38819833155945815 1105 48-49 -0.27692108734611054 1105 50-51 -0.24985635228085812 1105 52-53 -0.20473002864648748 1105 54-55 -0.10257386584402184 1105 56-57 0.01649300514763752 1105 58-59 0.029950463144004402 1105 60-61 -0.02239103770941142 1105 62-63 0.006080501217901713 1105 64-65 -0.03280193123255515 1105 66-67 0.2035381076164633 1105 68-69 0.33987144791326784 1105 70-71 0.27914539786710435 1105 72-73 0.3633956207598352 1105 74-75 0.3911595245761035 1105 76-77 0.31142570677815584 1105 78-79 0.3395359338055677 1105 80-81 0.21008400486607925 1105 82-83 0.22607771601505178 1105 84-85 0.33290795441955723 1105 86-87 0.2672987360096677 1105 88-89 0.562888626488455 1105 90-91 0.8097697247824449 1105 92-93 0.9214098023816852 1105 94-95 1.2352546185395 1105 96-97 1.24538712670034 1105 98-99 1.373182143475077 1105 100-101 1.6624227095019428 1106 1 -0.14063368150023692 1106 2 -0.1278278240166273 1106 3 -0.03909956270812032 1106 4 0.028237337825181896 1106 5 -0.05483634470196108 1106 6 -0.03283026792411903 1106 7 -0.04560054102171307 1106 8 -0.18371908646372503 1106 9 -0.2477315419954209 1106 10-11 -0.28006129640037614 1106 12-13 -0.32117436971799407 1106 14-15 -0.2052974964123564 1106 16-17 -0.06824907023864313 1106 18-19 -0.03931982899930375 1106 20-21 -0.05576691496825248 1106 22-23 -0.028606777753388712 1106 24-25 -0.26312344385680575 1106 26-27 -0.2888009260188582 1106 28-29 -0.14407479566428805 1106 30-31 -0.10948065314162392 1106 32-33 -0.08374065864298075 1106 34-35 -0.014879280050415389 1106 36-37 -0.192260747844486 1106 38-39 -0.3598691551241231 1106 40-41 -0.115146120166159 1106 42-43 -0.38624561744878605 1106 44-45 -0.11754606215649233 1106 46-47 -0.19677142128112024 1106 48-49 -0.29195716184381126 1106 50-51 0.02564474656557536 1106 52-53 0.05320374031682462 1106 54-55 0.09302946036502391 1106 56-57 -0.001700809332973563 1106 58-59 -0.1547903174722336 1106 60-61 0.08380776199850715 1106 62-63 0.3748029967452311 1106 64-65 0.40226130795709025 1106 66-67 0.3697277487902504 1106 68-69 0.38921071874826296 1106 70-71 0.2632163440831441 1106 72-73 0.3240876541676059 1106 74-75 0.4076684263870227 1106 76-77 0.5149432590066745 1106 78-79 0.4866172395584769 1106 80-81 0.2960505278032777 1106 82-83 0.12340663974960364 1106 84-85 0.042922647160061445 1106 86-87 0.2748611890399779 1106 88-89 0.5452304663322529 1106 90-91 0.49150711025635374 1106 92-93 0.6724202614099077 1106 94-95 0.7286257852354829 1106 96-97 0.7917962884324119 1106 98-99 1.0353670357135059 1106 100-101 1.4036816837105839 1107 1 -0.05383251898671304 1107 2 -0.14758642826695478 1107 3 -0.12007165504909878 1107 4 -0.09492537739568263 1107 5 -0.11176696061409075 1107 6 -0.07991252400714188 1107 7 -0.10726234860188555 1107 8 -0.04423763350518328 1107 9 0.05254762627566123 1107 10-11 -0.04203115172794725 1107 12-13 -0.16394441574179552 1107 14-15 -0.0796933194315983 1107 16-17 -0.06534232802692941 1107 18-19 0.02309192815638994 1107 20-21 0.036744820184033244 1107 22-23 0.11118363976132883 1107 24-25 0.07042442272918237 1107 26-27 -0.12324657759484836 1107 28-29 -0.06994736841306093 1107 30-31 -0.04071862002648885 1107 32-33 -0.0011201441298922532 1107 34-35 -0.07952461257695376 1107 36-37 -0.04641412078395035 1107 38-39 -0.07155227850824986 1107 40-41 0.016620845380167282 1107 42-43 -0.01591721654664724 1107 44-45 0.19212122794014874 1107 46-47 0.1700093880776663 1107 48-49 0.34052010129864385 1107 50-51 0.33535673108984554 1107 52-53 0.16216142042391368 1107 54-55 0.35561013953695664 1107 56-57 0.2703532548803125 1107 58-59 0.17788818519567684 1107 60-61 0.25175223089119214 1107 62-63 0.2585786903110616 1107 64-65 0.4071708189312915 1107 66-67 0.10178282149318107 1107 68-69 0.2491230259892241 1107 70-71 0.3773232806583131 1107 72-73 0.5060084143854375 1107 74-75 0.3009466136727177 1107 76-77 0.18951373409698036 1107 78-79 0.03547260380659978 1107 80-81 0.25994430633895504 1107 82-83 0.23307988910325506 1107 84-85 0.4630759375867477 1107 86-87 0.5975611450331577 1107 88-89 0.7780985192137813 1107 90-91 0.8200819768606742 1107 92-93 1.0733379869907012 1107 94-95 0.9418088077706415 1107 96-97 1.0930420023514067 1107 98-99 1.2753185823699056 1107 100-101 1.8292468963185406 1108 1 -0.05063099349494493 1108 2 -0.0972217822078072 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10-11 0.07982864637681786 2316 12-13 -0.08631176382829153 2316 14-15 -0.004599120404520818 2316 16-17 0.2161939596878284 2316 18-19 -0.10006275508180806 2316 20-21 0.01630398279686318 2316 22-23 0.05436194305408293 2316 24-25 0.004652412493818758 2316 26-27 0.07945953550021301 2316 28-29 0.18240584981936792 2316 30-31 0.21203916193791628 2316 32-33 0.17817786222502008 2316 34-35 -0.05751613466451744 2316 36-37 -0.13488985398981868 2316 38-39 0.043537491758605995 2316 40-41 -0.08543255281458784 2316 42-43 0.19828293529656804 2316 44-45 0.19776121894202703 2316 46-47 0.27053873037162646 2316 48-49 -0.23580466039187797 2316 50-51 -0.013692491416691155 2316 52-53 0.2328099877551253 2316 54-55 0.14589393168048304 2316 56-57 0.03531297425156765 2316 58-59 0.19522467255613662 2316 60-61 0.1398715543453406 2316 62-63 0.053666984324507894 2316 64-65 3.786472922300277E-4 2316 66-67 0.1361327779085606 2316 68-69 0.06512666741430095 2316 70-71 0.36217551472180887 2316 72-73 0.3681941833025739 2316 74-75 0.459038239801 2316 76-77 0.141030760219639 2316 78-79 -0.16827356496431278 2316 80-81 0.24241555454635844 2316 82-83 0.1721035935151427 2316 84-85 0.32358123803946626 2316 86-87 0.06209837473818425 2316 88-89 0.1702596136096446 2316 90-91 -0.04351958937389355 2316 92-93 0.4419261263490242 2316 94-95 0.48219804658626586 2316 96-97 0.5282285023654616 2316 98-99 0.6516925525074058 2316 100-101 -0.016694109783308875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 30.0 5 63.0 6 139.0 7 341.0 8 810.0 9 1446.0 10 2453.0 11 3782.0 12 5670.0 13 7641.0 14 10129.0 15 13384.0 16 17254.0 17 22230.0 18 20462.0 19 15548.0 20 14957.0 21 16014.0 22 17619.0 23 19674.0 24 23277.0 25 27769.0 26 33780.0 27 41319.0 28 50403.0 29 61378.0 30 74617.0 31 90345.0 32 111120.0 33 139300.0 34 177326.0 35 231344.0 36 295014.0 37 301225.0 38 122165.0 39 4184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.56936471556599 21.692584817278814 16.069185800752763 21.668864666402435 2 16.398603399919867 22.39979981997981 38.43628607305254 22.765310707047785 3 19.80794469520414 25.872710428355866 30.41495312333004 23.90439175310996 4 13.826097659686877 18.578448331318196 37.185971392289524 30.409482616705407 5 13.190922256255854 38.24204446276981 34.571629594097026 13.995403686877308 6 30.89428093989382 38.28751868456603 17.070969186358383 13.747231189181768 7 27.659084105493655 33.289299131860986 21.16923974647241 17.882377016172946 8 26.092695708640818 35.976584205533015 19.570636143941186 18.360083941884987 9 25.811825715737278 16.99686408273027 20.594330022494926 36.59698017903752 10-11 24.461445805712735 27.68522369076208 27.481421786112094 20.371908717413096 12-13 25.482271969857955 24.80138613695878 28.21432542457536 21.502016468607906 14-15 22.362569983228845 26.25769733206431 26.50787170446297 24.871860980243873 16-17 21.407004173287415 29.62395838767961 27.19897904137476 21.770058397658218 18-19 21.41407024434423 27.846296998869935 30.01750562119882 20.722127135587012 20-21 22.065440428968763 26.873762487824326 30.314052667809054 20.746744415397856 22-23 21.838046145535138 26.803929976058782 30.17970981311263 21.178314065293442 24-25 21.63934683647662 27.133552864517245 30.176749449272545 21.05035084973359 26-27 21.434282563663963 27.27893918261627 30.099581428249785 21.187196825469993 28-29 21.396623085460263 27.283479783139136 30.224438738527454 21.095458392873155 30-31 21.55818895105143 27.133392259708287 30.166344597726187 21.142074191514094 32-33 21.359491810195706 27.16288889350393 30.099006039338004 21.37861325696236 34-35 21.851026960986676 27.134624609276663 29.94937755258651 21.06497087715015 36-37 21.88492479569167 26.945399250945435 29.888507979345786 21.281167974017105 38-39 21.54328449611402 27.34342293112972 29.882892486443325 21.23040008631294 40-41 21.84175429194604 27.1901423396874 29.831006761654344 21.137096606712216 42-43 21.59798804559876 27.226885903299948 29.682710393763735 21.49241565733756 44-45 21.83755795965795 27.31119359665447 29.595971670808392 21.255276772879185 46-47 21.976291432122917 27.61058401203917 28.994065006840664 21.41905954899725 48-49 21.91761011800592 27.769557947607744 28.788541112123607 21.524290822262735 50-51 22.07402608519909 27.876790008018343 28.63460713404717 21.4145767727354 52-53 22.22246423023134 27.916096611173103 28.447267608573096 21.414171550022466 54-55 22.065719019583906 27.87686598727702 28.626350721271105 21.43106427186797 56-57 22.07384880026218 28.112984196820722 28.40102156645931 21.412145436457784 58-59 22.153019187801988 27.74656155864864 28.49976927631782 21.600649977231548 60-61 21.968668179835817 27.967078693744323 28.60809037276944 21.456162753650425 62-63 21.88440718196475 27.87329496211927 28.846994488464578 21.395303367451397 64-65 21.853179706649147 27.74174953893253 28.724363964962418 21.680706789455904 66-67 21.787964176286064 27.95109772300292 28.466566340276657 21.79437176043436 68-69 21.949648038747398 27.852881867955137 28.541329930813287 21.656140162484178 70-71 22.007822824473223 27.762517202970482 28.541963091302247 21.68769688125404 72-73 22.03666961635033 27.734886079232187 28.45002818830497 21.778416116112517 74-75 21.956942047580238 28.096142127814332 28.151910903682108 21.79500492092332 76-77 21.978292219268035 27.790857466456426 28.467402112122087 21.763448202153455 78-79 22.035428621791965 27.851539567718543 28.259370901868735 21.85366090862076 80-81 21.912555933425924 28.0838730396075 28.192067558764922 21.81150346820165 82-83 21.99206317246682 28.024563693560147 28.148790316351135 21.834582817621893 84-85 21.961440321140707 28.035077277648686 28.092999107238963 21.910483293971648 86-87 21.925309349551696 27.997217133018914 28.147580085937605 21.92989343149178 88-89 21.985079699709708 27.897329734280273 28.071398215905703 22.046192350104317 90-91 21.9463793213059 27.94606372974015 28.053278745617245 22.05427820333671 92-93 22.020398957637628 27.795510973095265 28.154649334821553 22.02944073444555 94-95 21.83132300657018 27.762517202970482 28.109970352893264 22.29618943756607 96-97 21.926955566822997 27.66032510005202 28.089303994533545 22.323415338591435 98-99 21.753122752276848 27.713957917557313 27.983558063437712 22.549361266728127 100-101 21.905094800084598 27.512021772801475 28.223817104414973 22.359066322698958 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 400.0 1 285.5 2 212.0 3 287.5 4 276.5 5 270.5 6 503.5 7 1124.0 8 1865.0 9 2273.5 10 2170.5 11 1938.0 12 2009.0 13 2213.0 14 2409.5 15 2536.0 16 2662.5 17 2804.0 18 3025.5 19 3336.0 20 3671.0 21 4239.0 22 4997.0 23 6128.5 24 7499.0 25 9242.0 26 12192.5 27 15562.5 28 18136.5 29 21891.5 30 26594.5 31 31201.0 32 36575.5 33 41298.5 34 45945.0 35 50665.0 36 56679.5 37 62369.5 38 64636.5 39 65650.5 40 66635.5 41 69111.5 42 70898.0 43 72247.0 44 74335.0 45 75513.0 46 74753.0 47 73817.5 48 73474.0 49 71786.0 50 69279.5 51 65260.0 52 61161.0 53 58958.0 54 56999.0 55 52261.5 56 46380.5 57 41141.0 58 36569.5 59 32111.5 60 26669.5 61 21590.5 62 17264.0 63 12815.5 64 9215.5 65 6877.0 66 5056.0 67 3742.5 68 2834.0 69 2099.0 70 1502.5 71 1097.0 72 813.5 73 664.0 74 537.5 75 404.5 76 279.5 77 171.0 78 113.0 79 73.0 80 42.5 81 35.0 82 24.5 83 9.5 84 6.5 85 5.0 86 2.0 87 2.0 88 3.5 89 2.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06164450520533901 2 0.0 3 0.0 4 0.0 5 0.0016208908517426855 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0130567823391785E-4 12-13 0.005242568848605248 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.331604889619866E-4 24-25 8.104454258713428E-4 26-27 4.8120197161110977E-4 28-29 0.002735253312315782 30-31 4.052227129356714E-4 32-33 0.0 34-35 0.0 36-37 5.065283911695892E-5 38-39 5.825076498450276E-4 40-41 1.0130567823391785E-4 42-43 0.011244930283964881 44-45 2.532641955847946E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.065283911695892E-5 82-83 4.3054913249415086E-4 84-85 5.318548107280688E-4 86-87 0.0 88-89 0.0 90-91 0.007724557965336236 92-93 0.0027099268927573026 94-95 0.0 96-97 0.0 98-99 1.5195851735087676E-4 100-101 0.010155894242950264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1974223.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.06171255755 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40322783974064 44.73822450575383 2 12.424535717341975 14.179305715279622 3 3.813597542218019 6.528312202926713 4 1.6819644899252935 3.8390309702449317 5 0.9208826319283525 2.6273570021167885 6 0.5525407385715028 1.8917352480422083 7 0.38669173229886294 1.5445704733773153 8 0.279975488844894 1.2780704694101512 9 0.2033438101674644 1.044283144151958 >10 1.1978047946344277 12.751186532659922 >50 0.09089457391670541 3.5301422083060467 >100 0.042589420611082715 4.288154940253178 >500 0.0014190689460485129 0.532761629220691 >1k 3.5476723651212746E-4 0.45532975948499577 >5k 1.7738361825606373E-4 0.7715351987716751 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8486 0.42983999274651347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6759 0.34236253959152535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4803 0.24328558627875368 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.065283911695892E-5 0.0 5 0.0 0.0 0.0 5.065283911695892E-5 0.0 6 0.0 0.0 0.0 5.065283911695892E-5 0.0 7 0.0 0.0 0.0 1.5195851735087676E-4 0.0 8 0.0 0.0 0.0 1.5195851735087676E-4 0.0 9 0.0 0.0 0.0 1.5195851735087676E-4 0.0 10-11 0.0 0.0 0.0 1.5195851735087676E-4 0.0 12-13 0.0 0.0 0.0 2.026113564678357E-4 0.0 14-15 0.0 0.0 0.0 2.026113564678357E-4 0.0 16-17 0.0 0.0 0.0 2.532641955847946E-4 0.0 18-19 0.0 0.0 0.0 3.798962933771919E-4 0.0 20-21 0.0 0.0 0.0 4.558755520526303E-4 0.0 22-23 0.0 0.0 0.0 6.58486908520466E-4 0.0 24-25 0.0 0.0 0.0 7.597925867543839E-4 0.0 26-27 0.0 0.0 0.0 9.370775236637401E-4 0.0 28-29 0.0 0.0 0.0 0.0012663209779239732 0.0 30-31 0.0 0.0 0.0 0.0022540513407046723 0.0 32-33 0.0 0.0 0.0 0.004837346135669577 0.0 34-35 0.0 0.0 0.0 0.008762941167233893 0.0 36-37 0.0 0.0 0.0 0.015322483832880075 0.0 38-39 0.0 0.0 0.0 0.025680989432298174 0.0 40-41 0.0 0.0 0.0 0.0379896293377192 0.0 42-43 0.0 0.0 0.0 0.05316015465324839 0.0 44-45 0.0 0.0 0.0 0.0706607105681577 0.0 46-47 0.0 0.0 0.0 0.09307459187741202 0.0 48-49 0.0 0.0 0.0 0.11969265883337393 0.0 50-51 0.0 0.0 0.0 0.15026164724045865 0.0 52-53 0.0 0.0 0.0 0.18703560843937084 0.0 54-55 0.0 0.0 0.0 0.22965997255629178 0.0 56-57 0.0 0.0 0.0 0.2813005420360314 0.0 58-59 0.0 5.065283911695892E-5 0.0 0.34291972082181194 0.0 60-61 0.0 5.065283911695892E-5 0.0 0.41203551979690234 0.0 62-63 0.0 5.065283911695892E-5 0.0 0.4853048515795835 0.0 64-65 0.0 5.065283911695892E-5 0.0 0.5649817675105598 0.0 66-67 0.0 5.065283911695892E-5 0.0 0.6611208561545479 0.0 68-69 0.0 5.065283911695892E-5 0.0 0.7789393599405943 0.0 70-71 0.0 5.065283911695892E-5 0.0 0.9110419643576232 0.0 72-73 0.0 5.065283911695892E-5 0.0 1.0550986388062544 0.0 74-75 0.0 5.065283911695892E-5 0.0 1.2050563690120113 0.0 76-77 0.0 5.065283911695892E-5 0.0 1.375072623508084 0.0 78-79 0.0 5.065283911695892E-5 0.0 1.5712257429885073 0.0 80-81 0.0 5.065283911695892E-5 0.0 1.7950099862072317 0.0 82-83 0.0 5.065283911695892E-5 0.0 2.0321412525332754 0.0 84-85 0.0 5.065283911695892E-5 0.0 2.274540414127482 0.0 86-87 0.0 5.065283911695892E-5 0.0 2.529526806242253 0.0 88-89 0.0 5.065283911695892E-5 0.0 2.8188051704392056 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4285 0.0 49.252224 1 GTATCAA 7745 0.0 42.408245 1 ATCAACG 10085 0.0 32.26267 3 TCAACGC 10275 0.0 31.666084 4 TATCAAC 10335 0.0 31.574165 2 CAACGCA 10460 0.0 31.106024 5 AACGCAG 10900 0.0 29.849611 6 ACGCAGA 12415 0.0 26.056017 7 CGCAGAG 12420 0.0 25.854296 8 GCAGAGT 13515 0.0 23.408081 9 CCGTGCG 105 0.002196364 22.619778 9 GAGTACT 8165 0.0 22.107265 12-13 GTACATG 7860 0.0 21.286968 1 AGAGTAC 12380 0.0 21.007341 10-11 GTACTTT 8565 0.0 20.823666 14-15 AGTACTT 8640 0.0 20.75443 12-13 TACATGG 8010 0.0 20.399126 2 ACATGGG 7525 0.0 20.135841 3 TGGTATC 2250 0.0 20.055187 2 CAGAGTA 13390 0.0 19.156704 10-11 >>END_MODULE