##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937141_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 612233 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.835794542273938 33.0 31.0 34.0 30.0 34.0 2 31.951284886636298 33.0 31.0 34.0 30.0 34.0 3 31.923016237282212 33.0 31.0 34.0 30.0 34.0 4 35.52500763598172 37.0 35.0 37.0 33.0 37.0 5 35.541313519526064 37.0 35.0 37.0 33.0 37.0 6 35.67103537378743 37.0 35.0 37.0 33.0 37.0 7 35.64277489125872 37.0 35.0 37.0 33.0 37.0 8 35.61728622926239 37.0 35.0 37.0 33.0 37.0 9 37.197517938431936 39.0 37.0 39.0 34.0 39.0 10-11 37.09694838403026 39.0 37.0 39.0 33.0 39.0 12-13 37.05111697670658 39.0 37.0 39.0 33.0 39.0 14-15 38.152021370948646 40.0 38.0 41.0 33.0 41.0 16-17 38.090139701714875 40.0 38.0 41.0 33.0 41.0 18-19 38.138336221667245 40.0 38.0 41.0 33.5 41.0 20-21 38.05998369901655 40.0 38.0 41.0 33.0 41.0 22-23 37.94522346884274 40.0 37.5 41.0 33.0 41.0 24-25 38.00573066136585 40.0 38.0 41.0 33.0 41.0 26-27 37.93149503538685 40.0 37.0 41.0 33.0 41.0 28-29 37.86514856272041 40.0 37.0 41.0 33.0 41.0 30-31 37.66186892898619 40.0 37.0 41.0 33.0 41.0 32-33 37.37448732753707 40.0 36.0 41.0 33.0 41.0 34-35 37.15069181177754 39.0 35.5 41.0 32.0 41.0 36-37 36.9003198128817 39.0 35.0 41.0 31.0 41.0 38-39 36.69868824450822 39.0 35.0 41.0 31.0 41.0 40-41 36.61421468623874 39.0 35.0 41.0 31.0 41.0 42-43 36.3288282402288 39.0 35.0 41.0 30.0 41.0 44-45 35.957116816636805 39.0 35.0 41.0 30.0 41.0 46-47 35.452406191760325 39.0 35.0 40.5 27.5 41.0 48-49 35.017825729746676 39.0 35.0 40.0 24.5 41.0 50-51 34.25536029583508 38.0 34.0 40.0 23.5 40.5 52-53 34.494796915553394 38.5 34.5 40.0 24.0 40.5 54-55 34.9516613772861 39.0 35.0 40.5 24.0 41.0 56-57 34.86271893217125 39.0 35.0 41.0 23.0 41.0 58-59 34.673904379541774 39.0 35.0 41.0 22.5 41.0 60-61 34.386900085425 39.0 35.0 40.0 22.0 41.0 62-63 34.11896451187701 38.0 34.0 40.0 21.0 41.0 64-65 33.83519264724377 38.0 34.0 40.0 19.5 41.0 66-67 33.4093939725562 37.0 34.0 40.0 17.0 41.0 68-69 32.97354111914908 37.0 33.0 39.0 13.5 41.0 70-71 32.526970940801945 36.0 33.0 39.0 10.0 40.5 72-73 32.100956661924464 36.0 32.0 38.5 7.5 40.0 74-75 31.639065519173258 35.0 32.0 37.5 4.0 40.0 76-77 31.216961516285465 35.0 31.5 37.0 2.0 39.0 78-79 30.749185359168813 35.0 31.0 36.5 2.0 39.0 80-81 30.232407433117785 35.0 31.0 36.0 2.0 38.5 82-83 29.76764401788208 34.5 30.0 36.0 2.0 37.0 84-85 29.35376890824245 34.0 30.0 35.0 2.0 37.0 86-87 29.111847123562434 34.0 30.0 35.0 2.0 36.0 88-89 28.68246239585256 34.0 29.0 35.0 2.0 36.0 90-91 28.278933020598366 34.0 29.0 35.0 2.0 36.0 92-93 27.945341071128148 34.0 29.0 35.0 2.0 35.0 94-95 27.529256835224498 34.0 27.0 35.0 2.0 35.0 96-97 27.016118046560706 34.0 26.0 35.0 2.0 35.0 98-99 26.51066505725761 34.0 25.0 35.0 2.0 35.0 100-101 24.09964261972158 31.5 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5040855741259129 1101 2 0.535248125565861 1101 3 0.41508499435858326 1101 4 0.4046729953794639 1101 5 0.47489033544403725 1101 6 0.3196837318616872 1101 7 0.32614010504200763 1101 8 0.28082374950930244 1101 9 0.6302328290879728 1101 10-11 0.5739733351335161 1101 12-13 0.5312301364819234 1101 14-15 0.4649058910167554 1101 16-17 0.5677384936279424 1101 18-19 0.5583034261914932 1101 20-21 0.5742939440997858 1101 22-23 0.7001703437156479 1101 24-25 0.5327139571071697 1101 26-27 0.7264334136342185 1101 28-29 0.7255441290644384 1101 30-31 0.7619537026713914 1101 32-33 0.7757546409106268 1101 34-35 0.8205935439706948 1101 36-37 0.5987425197405543 1101 38-39 0.8277439510242033 1101 40-41 0.7417307271841196 1101 42-43 0.6861167432666164 1101 44-45 0.7718444297850269 1101 46-47 0.8079376457292184 1101 48-49 0.7951753985282011 1101 50-51 0.8668402982740062 1101 52-53 0.6698538435794532 1101 54-55 0.597464298044855 1101 56-57 0.433513277742243 1101 58-59 0.5627287232020066 1101 60-61 0.785328407494184 1101 62-63 0.7284808332333341 1101 64-65 0.7603026027548054 1101 66-67 0.9198213109459203 1101 68-69 1.0285375024563805 1101 70-71 1.0263388372981908 1101 72-73 0.9597216477757442 1101 74-75 0.7418194981751896 1101 76-77 0.9645328274758107 1101 78-79 0.9830083941700565 1101 80-81 0.6350634921760978 1101 82-83 0.7662685397193165 1101 84-85 0.8227310017909701 1101 86-87 0.9444953583508031 1101 88-89 0.7600843340733547 1101 90-91 0.9175628064650674 1101 92-93 0.9636482694372361 1101 94-95 1.0352164228862435 1101 96-97 1.1421711315410334 1101 98-99 0.7697762510029982 1101 100-101 0.9864970483459032 1102 1 0.09672626676660911 1102 2 0.2976507229684593 1102 3 0.13566940994299515 1102 4 0.15573360144006898 1102 5 0.31311544366914745 1102 6 0.14342832060627586 1102 7 0.2108154297173357 1102 8 0.3018410655266237 1102 9 0.4520726559278003 1102 10-11 0.4873499585101442 1102 12-13 0.4138816516334458 1102 14-15 0.4396245057353738 1102 16-17 0.556244987134427 1102 18-19 0.49072766861573314 1102 20-21 0.4923675371733722 1102 22-23 0.6005707766160882 1102 24-25 0.4448784592716706 1102 26-27 0.5517256214264279 1102 28-29 0.2792995403198475 1102 30-31 0.387960196177886 1102 32-33 0.6058195759755591 1102 34-35 0.4992082625854124 1102 36-37 0.5262858097838432 1102 38-39 0.6982526090328633 1102 40-41 0.8434082163616097 1102 42-43 1.0078375224873994 1102 44-45 0.7033271137677062 1102 46-47 0.769809940101517 1102 48-49 0.6735195543723478 1102 50-51 0.5639723329060402 1102 52-53 0.8275161812417906 1102 54-55 0.9743041248846822 1102 56-57 0.8882968275257923 1102 58-59 0.788507943981223 1102 60-61 0.957817584983367 1102 62-63 0.9998011795536783 1102 64-65 1.0269151568673607 1102 66-67 0.6978299689545793 1102 68-69 0.9169574158762899 1102 70-71 0.860678664138014 1102 72-73 0.769689180243283 1102 74-75 0.6821441734998643 1102 76-77 0.9774440828870716 1102 78-79 0.8564824201440828 1102 80-81 1.007422799535405 1102 82-83 1.0988226522734301 1102 84-85 1.0828825169424832 1102 86-87 0.8450040163594643 1102 88-89 0.9957769747659952 1102 90-91 0.9781039320061886 1102 92-93 0.9143084425974095 1102 94-95 0.9376947778645928 1102 96-97 0.9497577116276155 1102 98-99 0.8532935670203159 1102 100-101 0.9574169617658193 1103 1 0.27741633940583554 1103 2 0.40652701616678755 1103 3 0.4402313734032681 1103 4 0.34313729943698945 1103 5 0.4965570021107055 1103 6 0.4665352983742679 1103 7 0.4046300896336916 1103 8 0.4333070571621036 1103 9 0.5854922024829392 1103 10-11 0.61445484514892 1103 12-13 0.5644979844531619 1103 14-15 0.8149546840316546 1103 16-17 0.806767769436874 1103 18-19 0.632195311142695 1103 20-21 0.6182204977721 1103 22-23 0.7646143601511923 1103 24-25 0.492919401380405 1103 26-27 0.8381552934493186 1103 28-29 0.8215623622436823 1103 30-31 0.8097079399595302 1103 32-33 0.7940212048551558 1103 34-35 0.8185044325171802 1103 36-37 1.098209648656841 1103 38-39 1.0684925385948247 1103 40-41 0.9483498848626013 1103 42-43 0.9150574212327243 1103 44-45 1.0210136645051051 1103 46-47 0.7768950160425234 1103 48-49 0.9106838731044746 1103 50-51 0.9720727071081114 1103 52-53 1.030108080867592 1103 54-55 0.9025041028727898 1103 56-57 0.748246200187026 1103 58-59 1.0305561500124796 1103 60-61 1.0183227577766658 1103 62-63 0.9128542281999543 1103 64-65 0.9539595108184926 1103 66-67 0.9853464778693919 1103 68-69 0.9590138763649563 1103 70-71 1.10397879620934 1103 72-73 0.9656499990854499 1103 74-75 0.8857678803015432 1103 76-77 0.9772652363099219 1103 78-79 0.9645607824597313 1103 80-81 0.9221680119501094 1103 82-83 0.9290009485534227 1103 84-85 0.9223470778053517 1103 86-87 0.6843310029322147 1103 88-89 0.7927448374118242 1103 90-91 0.8239932122175055 1103 92-93 0.9806192504336408 1103 94-95 1.1304501157470526 1103 96-97 0.9108241875245966 1103 98-99 1.2106840578853806 1103 100-101 1.6094503097737416 1104 1 0.29312472920061694 1104 2 0.2746298875442186 1104 3 0.41502317055642024 1104 4 0.24216397940831058 1104 5 0.4362787950009661 1104 6 0.23506755463345286 1104 7 0.2794940463093951 1104 8 0.3298474486334655 1104 9 0.3266109846778775 1104 10-11 0.43111012029580564 1104 12-13 0.45424275884153076 1104 14-15 0.578319080094559 1104 16-17 0.356594753287915 1104 18-19 0.5201027564336371 1104 20-21 0.5544974474485755 1104 22-23 0.555743501881544 1104 24-25 0.6025542456182507 1104 26-27 0.3551389777764129 1104 28-29 0.4564006463235799 1104 30-31 0.4987813688228613 1104 32-33 0.37863717568651367 1104 34-35 0.41180812408403966 1104 36-37 0.449663436793621 1104 38-39 0.37744127199277955 1104 40-41 0.317780443825022 1104 42-43 0.2301623383207172 1104 44-45 0.14827281721418473 1104 46-47 0.15991085541494243 1104 48-49 0.2826483506147497 1104 50-51 0.44381737194737525 1104 52-53 0.3791505978298346 1104 54-55 0.38313534389751425 1104 56-57 0.43987855337336157 1104 58-59 0.4805958020273522 1104 60-61 0.7270697779217983 1104 62-63 0.7016518790859863 1104 64-65 0.7065416547898451 1104 66-67 0.7451600538619374 1104 68-69 0.8820472912250565 1104 70-71 0.6435438862420355 1104 72-73 0.5583190202641575 1104 74-75 0.5246904409881736 1104 76-77 0.5239712612728233 1104 78-79 0.22695352554563542 1104 80-81 0.3698535488479173 1104 82-83 0.3474225840263756 1104 84-85 0.32098319138396647 1104 86-87 0.5660203454708466 1104 88-89 0.5961327627183834 1104 90-91 0.2745746560271449 1104 92-93 0.3116016439864353 1104 94-95 0.5317439859980375 1104 96-97 0.3840322855853415 1104 98-99 0.30537259309470244 1104 100-101 0.5324372853371457 1105 1 0.13747003220362686 1105 2 0.3574567145229146 1105 3 0.23465554650582376 1105 4 0.299417371657583 1105 5 0.35391896530089184 1105 6 0.2466571469946075 1105 7 0.04564930749599938 1105 8 0.3304045266054203 1105 9 0.25687904585689125 1105 10-11 0.2600622921887279 1105 12-13 0.32408800969255935 1105 14-15 0.19786090124171096 1105 16-17 0.37025383105125087 1105 18-19 0.4271122196475261 1105 20-21 0.39597083571532465 1105 22-23 0.4018216729590094 1105 24-25 0.1985933027104423 1105 26-27 0.1782892418550759 1105 28-29 0.17913819859408164 1105 30-31 -0.18990212554946595 1105 32-33 0.21369942618670024 1105 34-35 -0.04392077095773317 1105 36-37 0.1352711495974077 1105 38-39 0.4456631739280894 1105 40-41 0.2757542445665351 1105 42-43 0.15499711136477856 1105 44-45 -0.01695413872213436 1105 46-47 -0.1126247264589253 1105 48-49 -0.16522337447793944 1105 50-51 -0.11815458925155298 1105 52-53 -0.18729339568434966 1105 54-55 -0.2519477673948174 1105 56-57 -0.1880715893334255 1105 58-59 -0.15773139949738635 1105 60-61 -0.15934439414180446 1105 62-63 -0.2838913549057267 1105 64-65 -0.1167935117646195 1105 66-67 -0.18321038639559362 1105 68-69 0.036385150718139414 1105 70-71 -0.0013094244604943128 1105 72-73 -0.038851971856153966 1105 74-75 -0.18834921348125278 1105 76-77 -0.23489457538716962 1105 78-79 -0.132681789879026 1105 80-81 0.08362688685912545 1105 82-83 0.013620277960626481 1105 84-85 -0.2749325973910359 1105 86-87 -0.28526946782506357 1105 88-89 -0.1630649501802246 1105 90-91 -0.21747809333043477 1105 92-93 -0.35138751788382905 1105 94-95 -0.14133776934279751 1105 96-97 -0.25098326518698144 1105 98-99 -0.4164558553364692 1105 100-101 -0.16395284940460542 1106 1 0.025571517901017415 1106 2 0.23488667978272915 1106 3 0.3631572835152106 1106 4 0.26101837690556806 1106 5 0.3534847129540779 1106 6 0.29665160334762675 1106 7 0.28071841829502375 1106 8 0.2910446330434411 1106 9 0.2040480901321473 1106 10-11 0.34514803392869453 1106 12-13 0.3495333493333277 1106 14-15 0.49383159382800557 1106 16-17 0.38803969844721564 1106 18-19 0.08841386795856465 1106 20-21 0.438952578637938 1106 22-23 0.2929213477317134 1106 24-25 0.41836656754893653 1106 26-27 0.17902377507999745 1106 28-29 0.4325521611929446 1106 30-31 0.3891826183340399 1106 32-33 0.09620644813954016 1106 34-35 0.2198505720831463 1106 36-37 0.36074051170842125 1106 38-39 0.05354716387560643 1106 40-41 0.2357568316017904 1106 42-43 0.38976132157987564 1106 44-45 0.32481631733522676 1106 46-47 0.10771676219507498 1106 48-49 0.2533681696125356 1106 50-51 0.2926435111254122 1106 52-53 0.5222333616517432 1106 54-55 0.4981169084866153 1106 56-57 0.5375594624811981 1106 58-59 0.3975480003104366 1106 60-61 0.22510752396004818 1106 62-63 0.3467539256028047 1106 64-65 0.5154732854857258 1106 66-67 0.23836548765275012 1106 68-69 0.1732162173158187 1106 70-71 0.2632565079809197 1106 72-73 0.25679996102875435 1106 74-75 0.3690182933559143 1106 76-77 0.13142037767661563 1106 78-79 0.25219514115800834 1106 80-81 0.21788477731665878 1106 82-83 0.06131874052253039 1106 84-85 0.12546192548575164 1106 86-87 -0.10542432036405458 1106 88-89 -0.004604420946723309 1106 90-91 -0.20190506502087757 1106 92-93 -0.07335976269127542 1106 94-95 -0.2890205249049238 1106 96-97 -0.33314011343888694 1106 98-99 -0.5279847931737081 1106 100-101 -0.41132423679465546 1107 1 0.11664776318064085 1107 2 0.13793469273003822 1107 3 0.20702529286604587 1107 4 0.07367797050383729 1107 5 0.2905868528569755 1107 6 0.16575338360297565 1107 7 0.10106547817633782 1107 8 -0.04527077785387945 1107 9 0.14849153555563532 1107 10-11 0.190652439611128 1107 12-13 0.3111679474271938 1107 14-15 0.27179643828044675 1107 16-17 0.083260881687643 1107 18-19 0.014286013256167962 1107 20-21 0.4199407102689392 1107 22-23 0.2462773088897805 1107 24-25 0.15365923073900944 1107 26-27 -0.008305392335927309 1107 28-29 0.04167596986044941 1107 30-31 0.32654325491019875 1107 32-33 0.07075464091062145 1107 34-35 0.0765512554134844 1107 36-37 -0.03888185836890301 1107 38-39 0.09596534405903157 1107 40-41 0.20771331424879946 1107 42-43 0.30622868356513067 1107 44-45 0.3414837332676157 1107 46-47 0.17873366562971427 1107 48-49 0.2980858462893963 1107 50-51 0.3746139301148119 1107 52-53 0.7275404107436287 1107 54-55 0.8391931537662458 1107 56-57 0.921597854856671 1107 58-59 0.7815346933512544 1107 60-61 0.890900546797667 1107 62-63 0.953978345671139 1107 64-65 1.14512847340157 1107 66-67 1.1908785248762683 1107 68-69 0.9681519303170774 1107 70-71 0.632284110930037 1107 72-73 0.745430602999626 1107 74-75 0.6390956175781781 1107 76-77 0.7648810861822817 1107 78-79 0.8596128717819944 1107 80-81 0.4541057807333111 1107 82-83 0.43378097753026523 1107 84-85 0.17028075303475276 1107 86-87 0.4451421245199576 1107 88-89 0.5101838365609161 1107 90-91 0.49149315472377353 1107 92-93 0.6943821002830077 1107 94-95 0.9432263731349977 1107 96-97 0.9183029723370559 1107 98-99 0.6975996340875739 1107 100-101 0.8380890881469014 1108 1 -0.4827138359035814 1108 2 -0.1253418449356154 1108 3 -0.28102120033168276 1108 4 -0.09818836155269395 1108 5 0.005406559632831431 1108 6 -0.054829542474600146 1108 7 -0.2053053226866055 1108 8 -0.18305530653789504 1108 9 -0.4289707107350367 1108 10-11 -0.2151195852204708 1108 12-13 -0.04522266587795798 1108 14-15 -0.13563983169710525 1108 16-17 -0.061524043245221094 1108 18-19 0.07555268872836507 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-1.6824948903937056 2316 60-61 -1.5126799407467217 2316 62-63 -1.3342949568680424 2316 64-65 -1.4146824896721455 2316 66-67 -1.463719237652768 2316 68-69 -1.5166249900898343 2316 70-71 -1.3272598508353788 2316 72-73 -1.7444420373763094 2316 74-75 -1.9787574249017332 2316 76-77 -2.0963556638777945 2316 78-79 -2.1265577954542714 2316 80-81 -2.61832499917751 2316 82-83 -1.938895443584201 2316 84-85 -2.0994946988649623 2316 86-87 -2.413369877188849 2316 88-89 -2.0755209133923564 2316 90-91 -1.7043095847096517 2316 92-93 -2.415530323287868 2316 94-95 -1.9908897190338521 2316 96-97 -2.183176214910965 2316 98-99 -2.412112538502072 2316 100-101 -2.229966875327321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1107.0 3 329.0 4 293.0 5 323.0 6 359.0 7 455.0 8 609.0 9 772.0 10 1024.0 11 1490.0 12 2195.0 13 3185.0 14 3722.0 15 4836.0 16 6721.0 17 9805.0 18 8267.0 19 3575.0 20 3396.0 21 3252.0 22 3589.0 23 4057.0 24 4689.0 25 5374.0 26 6269.0 27 7353.0 28 8913.0 29 10709.0 30 13021.0 31 16104.0 32 20503.0 33 26371.0 34 36190.0 35 53005.0 36 74118.0 37 117360.0 38 127264.0 39 21629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.30645419524325 24.288267564997764 16.19691618008881 19.208362059670183 2 13.793604769877895 25.089557724506882 39.595213786907344 21.521623718707886 3 17.75404458848436 29.74193695930582 34.44385274822927 18.060165703980555 4 10.067846506468534 20.813504607779233 43.96136686287099 25.157282022881244 5 9.198682120374183 41.64247939178809 37.32390683323201 11.834931654605715 6 22.4005830132044 43.89412950801725 19.163454483956517 14.541832994821835 7 21.31086215740278 38.36843841989539 25.00547240763066 15.315227015071173 8 22.546566901264605 34.13882075726058 22.4730337647822 20.84157857669261 9 23.57650257154247 19.574110742969918 26.28887538311735 30.560511302370262 10-11 21.011243113960283 29.16176557078163 30.388509870807113 19.43848144445098 12-13 23.56422831177807 29.498808476361106 29.516291236643866 17.420671975216962 14-15 20.93859631210308 30.13424271507627 30.60782912335872 18.319331849461925 16-17 16.155868044551465 33.31280446168071 31.28444848124467 19.24687901252316 18-19 15.978057737294005 32.593293822449326 34.43327800938329 16.995370430873376 20-21 17.70819720334553 30.25009801359122 33.74436421850497 18.297340564558283 22-23 17.577529668319215 30.012904595832957 34.00336499587543 18.406200739972395 24-25 18.533754421383694 31.2454520285484 33.451019113118 16.769774436949906 26-27 17.46761445397422 29.888947508546583 36.09524478425698 16.54819325322222 28-29 16.136124380226374 31.5701313009468 35.17823264368417 17.11551167514266 30-31 17.18022386901719 29.985234379721447 34.54444631374003 18.290095437521337 32-33 17.351702216973656 30.731129263300943 34.867966502839614 17.049202016885783 34-35 18.374050402379485 30.201573583913316 34.23810869391228 17.186267319794915 36-37 18.37462208015576 29.303304460883357 34.77499579408494 17.547077664875953 38-39 16.742495702204636 31.768894986790148 34.00995536826287 17.47865394274234 40-41 16.526142824708895 31.465798152010755 34.81615659397648 17.191902429303877 42-43 16.56420023095782 31.43549922986837 32.91083623391748 19.08946430525633 44-45 17.634299359884224 30.15110260309392 33.198308487128266 19.016289549893585 46-47 19.076234048148336 31.609207605601135 32.55296594597155 16.76159240027898 48-49 17.892140649181478 31.384645539072164 32.62829072288714 18.094923088859215 50-51 15.891103461511763 31.73859603990314 33.91628180470654 18.454018693878552 52-53 17.43998588767965 33.38282445678183 31.183246193021912 17.993943462516597 54-55 17.425119135608377 31.30700031442817 31.705548227972674 19.56233232199078 56-57 18.525130649322435 31.773082196982998 30.920298809026992 18.781488344667572 58-59 19.300797417946967 30.647832663101532 31.6098956921137 18.4414742268378 60-61 18.472752516487333 31.972313534924556 32.33387100067379 17.22106294791433 62-63 17.338495952853663 31.947260917821406 32.58787873343226 18.126364395892665 64-65 17.5490363420608 32.753916251309775 31.842485885488088 17.854561521141346 66-67 18.043766621580122 31.682773672070518 30.19940995432537 20.074049752023996 68-69 17.370145384240278 31.00631966889738 31.965256115261877 19.658278831600466 70-71 19.005564668439867 31.675641586992466 31.50090838392618 17.81788536064149 72-73 18.930347710099184 29.810572366975503 33.131825002225824 18.127254920699485 74-75 17.296983806081396 33.4110968163532 31.012354755049117 18.279564622516286 76-77 17.62611174150984 31.488865633575223 31.61957465650077 19.265447968414165 78-79 17.759436967623678 31.65782844372127 31.688214940354246 18.894519648300808 80-81 18.73934776889377 31.200844498260512 30.913816403332895 19.145991329512828 82-83 17.868276837912113 31.509282285393102 32.20418740380311 18.418253472891674 84-85 17.469887391177206 31.598839441350457 31.255033743438776 19.67623942403356 86-87 17.416934444413588 32.25160380764437 31.220135361855274 19.111326386086773 88-89 18.01611513376868 31.391594483445846 30.56492814349248 20.02736223929299 90-91 17.937097382896933 29.753026112215707 32.03880481433256 20.271071690554805 92-93 19.201667216741445 31.272077877517408 31.849247922514618 17.67700698322653 94-95 18.540017805656973 29.85494106980961 32.27389674352503 19.33114438100839 96-97 17.47016737635461 30.03446199990688 32.76299635612748 19.73237426761103 98-99 17.96724257648164 31.11252705959237 30.734795572438017 20.18543479148797 100-101 17.35381488174798 30.07468978656793 31.959908365713613 20.611586965970478 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3453.0 1 2177.5 2 878.5 3 786.0 4 639.0 5 702.5 6 1080.5 7 1871.5 8 2730.0 9 2845.5 10 2263.0 11 1728.0 12 1691.0 13 1623.5 14 1447.5 15 1373.5 16 1320.5 17 1349.0 18 1428.0 19 1620.5 20 1871.5 21 2149.5 22 2437.5 23 2811.5 24 3158.0 25 4197.0 26 7008.0 27 9771.5 28 10018.0 29 10464.0 30 13346.0 31 16036.0 32 19485.0 33 24723.0 34 30737.0 35 33905.0 36 36529.0 37 41096.0 38 38302.5 39 31555.0 40 26924.0 41 23164.5 42 20070.5 43 18287.0 44 17602.0 45 16273.0 46 14669.5 47 18486.5 48 30671.0 49 28483.0 50 12679.5 51 4397.0 52 1932.5 53 1239.5 54 851.5 55 664.5 56 551.0 57 507.0 58 505.0 59 523.0 60 531.0 61 492.5 62 384.0 63 308.5 64 241.0 65 180.5 66 160.5 67 133.0 68 102.0 69 79.0 70 54.5 71 44.5 72 41.5 73 30.0 74 19.0 75 18.0 76 17.5 77 10.0 78 7.5 79 6.5 80 3.5 81 2.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02205042851332744 2 0.009473517435355494 3 0.009310180927849365 4 0.018947034870710987 5 0.05569774905959005 6 0.03920076180147101 7 0.011596892032935173 8 0.04312083798161811 9 0.024337139618413252 10-11 0.020417063438266148 12-13 0.032912306262485036 14-15 0.01559863646683534 16-17 0.014210276153033241 18-19 0.013230257107996465 20-21 0.011923565047947431 22-23 0.007840152360294202 24-25 0.0015516968213082274 26-27 0.0 28-29 0.0022050428513327443 30-31 0.0 32-33 3.266730150122584E-4 34-35 0.0 36-37 0.0 38-39 8.16682537530646E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 8.16682537530646E-5 50-51 8.16682537530646E-5 52-53 3.266730150122584E-4 54-55 0.0017150333288143567 56-57 4.08341268765323E-4 58-59 0.0011433555525429044 60-61 0.0033483984038756485 62-63 0.0018783698363204857 64-65 0.0013883603138020982 66-67 0.012576911077971948 68-69 0.015761972974341468 70-71 0.025643831678462285 72-73 0.016251982496859857 74-75 0.019682049154488568 76-77 0.030462258649893097 78-79 0.019437044393229377 80-81 0.04442753004166714 82-83 0.03381065705376874 84-85 0.01862036185569873 86-87 0.015680304720588404 88-89 0.012331906316712755 90-91 0.023520457080882604 92-93 0.0318506189636952 94-95 0.011106882510416785 96-97 0.01788534757192115 98-99 0.025807168185968413 100-101 0.038384079263940364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 612233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.454544621767553 #Duplication Level Percentage of deduplicated Percentage of total 1 72.04830071384532 11.855219790184949 2 9.474857512572772 3.1180893145103643 3 3.959593110622913 1.9545990456836435 4 2.2475813579567823 1.4793171098221114 5 1.4787190902503387 1.216582462679186 6 1.1357840503374057 1.1213285602181238 7 0.8858315680119173 1.020316854425569 8 0.7338604635557797 0.9660271794983679 9 0.5788899296903254 0.8572833161263206 >10 5.502953666689875 19.18597100234452 >50 1.0687967786503378 12.404697102766027 >100 0.8018475364617127 25.87083960097311 >500 0.052989924479389994 5.957715987368624 >1k 0.026994867187613767 9.564689064715976 >5k 0.002999429687512641 3.4273236086831202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8650 1.4128607899280177 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6324 1.032940073468761 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5859 0.9569885974784109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4877 0.7965921471073922 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4395 0.7178639504894379 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4368 0.7134538647867723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3398 0.555017452505827 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3015 0.4924595701309795 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2861 0.4673057479750356 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2686 0.43872185916146306 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2654 0.4334950909212669 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2479 0.40491120210769427 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2363 0.3859641672369833 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2317 0.3784506878917014 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2280 0.3724072371139746 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2274 0.3714272180689378 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1745 0.28502220559819547 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1652 0.26983191040012544 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1586 0.2590517009047209 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1405 0.2294877930461115 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1401 0.22883444701608702 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1371 0.22393435179090312 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1316 0.21495084387806604 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1166 0.19045036775214666 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1163 0.18996035822962828 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1138 0.18587694554197504 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1106 0.1806501773017789 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1048 0.17117665986642341 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1046 0.17084998685141115 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.16807326622380694 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 985 0.16088645989353725 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 977 0.15957976783348823 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 937 0.15304630753324305 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 913 0.14912623135309597 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 892 0.14569616469546726 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 887 0.14487948215793658 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 855 0.13965271391774048 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 848 0.13850935836519757 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 824 0.13458928218505045 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 823 0.13442594567754435 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 789 0.12887250442233594 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 785 0.12821915839231143 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 783 0.12789248537729916 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 775 0.12658579331725012 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 768 0.12544243776470723 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 766 0.12511576474969496 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 758 0.12380907268964593 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 742 0.12119568856954786 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 740 0.1208690155545356 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 727 0.11874564095695594 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 723 0.11809229492693141 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 711 0.11613225683685785 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 691 0.11286552668673527 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 679 0.11090548859666173 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 660 0.10780209495404529 No Hit ACCTAAAAACAATTTTATGAATCAACTCGTCTATGTGGCAAAATAGTGAG 644 0.1051887108339472 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 643 0.10502537432644109 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 636 0.10388201877389816 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 634 0.10355534575888592 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 632 0.10322867274387365 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 630 0.10290199972886141 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 624 0.10192198068382462 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 616 0.10061528862377558 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.16682537530646E-5 0.0 14-15 0.0 0.0 0.0 1.633365075061292E-4 0.0 16-17 0.0 0.0 0.0 2.450047612591938E-4 0.0 18-19 0.0 0.0 0.0 4.900095225183876E-4 0.0 20-21 0.0 0.0 0.0 8.166825375306461E-4 0.0 22-23 0.0 0.0 0.0 0.0015516968213082274 0.0 24-25 0.0 0.0 0.0 0.0021233745975796797 0.0 26-27 0.0 0.0 0.0 0.003266730150122584 0.0 28-29 0.0 0.0 0.0 0.007921820614047266 0.0 30-31 0.0 0.0 0.0 0.015680304720588404 0.0 32-33 0.0 0.0 0.0 0.024663812633425507 0.0 34-35 0.0 0.0 0.0 0.03585236339759536 0.0 36-37 0.0 0.0 0.0 0.0472859189230244 0.0 38-39 0.0 0.0 0.0 0.06811132363005588 0.0 40-41 0.0 0.0 0.0 0.0920401219797038 0.0 42-43 0.0 0.0 0.0 0.11686727112063544 0.0 44-45 0.0 0.0 0.0 0.13663098852887706 0.0 46-47 0.0 0.0 0.0 0.1667665741637579 0.0 48-49 0.0 0.0 0.0 0.1877553153782955 0.0 50-51 0.0 0.0 0.0 0.20768236929404327 0.0 52-53 0.0 0.0 0.0 0.2523549040969696 0.0 54-55 0.0 0.0 0.0 0.32904139437109725 0.0 56-57 0.0 0.0 0.0 0.4542388273745453 0.0 58-59 0.0 0.0 0.0 0.6061417793552455 0.0 60-61 0.0 0.0 0.0 0.7895686772846285 0.0 62-63 0.0 0.0 0.0 0.9015358531800801 0.0 64-65 0.0 0.0 0.0 1.0297550115723917 0.0 66-67 0.0 0.0 0.0 1.2533626903482824 0.0 68-69 0.0 0.0 0.0 1.5842824545557002 0.0 70-71 0.0 0.0 0.0 1.9054836965665032 0.0 72-73 0.0 0.0 0.0 2.225133241755998 0.0 74-75 0.0 0.0 0.0 2.563566485308698 0.0 76-77 0.0 0.0 0.0 2.841973562352895 0.0 78-79 0.0 0.0 0.0 3.116460563216945 0.0 80-81 0.0 0.0 0.0 3.3809023688693687 0.0 82-83 0.0 0.0 0.0 3.6935284442360996 0.0 84-85 0.0 0.0 0.0 4.216286936509466 0.0 86-87 0.0 0.0 0.0 4.874369725251661 0.0 88-89 0.0 0.0 0.0 5.6266976788248915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAGG 20 0.0020700775 71.28065 1 GCGTCAG 30 1.2979761E-4 63.36058 1 CGTCAGA 35 2.7821193E-4 54.304634 2 TATGCTC 40 5.379118E-4 47.512665 6 GGATTGT 45 9.6089236E-4 42.233482 8 GACATAT 295 0.0 40.271553 1 CATATAG 295 0.0 40.26826 3 GTAAGCG 30 8.284034E-5 39.589035 94-95 ATGTATT 50 0.001611816 38.016346 1 ATTGCCG 50 0.001613111 38.010136 8 AAAGACA 105 4.6371497E-8 36.203087 4 CTTTAGG 310 0.0 35.25421 2 CCTTTAG 300 0.0 34.848316 1 GCTTTGC 150 2.5465852E-11 34.848316 1 ATATTGC 55 0.0025753172 34.554665 6 ACATATA 350 0.0 33.94039 2 TAGGCAT 315 0.0 33.18616 5 CTTTGCG 160 5.2750693E-11 32.66763 2 GGCATTC 325 0.0 32.16242 7 TTAGGCA 345 0.0 31.6777 4 >>END_MODULE