FastQCFastQC Report
Thu 26 May 2016
SRR937141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612233
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99031.6175214338331974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65071.0628306543423827No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55060.8993308103287474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.8962274166861308No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.7439977916904185No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42190.6891167251683591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.6299889094511404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38240.6245988047034381No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30780.5027497701038657No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27540.44982874167187986No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27510.44933873214936143No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26570.43398510044378535No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25430.41536473858808654No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25400.4148747290655681No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23230.37943070693673814No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21010.3431700022703774No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18090.29547574207858773No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA16410.268035208817558No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16390.2677085358025457No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC15700.25643831678462287No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA14420.2355312438238383No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT14080.22997780256862993No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT13560.2214843041783112No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG11800.19273707885723246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.1907770407671589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.18930701219960375No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC11080.18097685031679117No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG11080.18097685031679117No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10980.17934348524172986No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT10630.17362670747901535No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10560.17248335192647243No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA10380.1695432947913621No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC9740.15908975831096986No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG9370.15304630753324305No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA8850.14455280914292434No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8790.14357279009788756No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT8730.1425927710528508No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC8690.14193942502282628No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT8540.13948937741023434No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA8410.13736600281265465No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT8280.13524262821507496No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8170.13344592663250757No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA7960.13001585997487886No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT7900.12903584092984208No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT7720.12609578379473174No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT7690.12560577427221337No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA7460.12184903459957239No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA7450.12168569809206625No Hit
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC7180.11727561238940078No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.11645892985187012No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA7000.11433555525429044No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6930.11319219970174754No Hit
AACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCA6890.11253885367172303No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC6800.11106882510416786No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6730.10992546955162494No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT6650.10861877749157592No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT6580.10747542193903302No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT6550.10698541241651463No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG6520.10649540289399623No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC6430.10502537432644109No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG6390.10437202829641656No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT6380.10420869178891043No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA6380.10420869178891043No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA6280.10257532671384914No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT6140.10028861560876332No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC250.00501745957.0077446
GAGCATA250.00501745957.0077446
ATGTATT704.298039E-640.726471
ATGGGCG801.0757834E-535.629845
AAAGACA1750.035.278984
ATACTCA1553.6379788E-1133.7142566
GGACAAT2550.031.6709676
GACAATG2650.030.4758387
GCAGAGA3600.030.34390694-95
CGCAGAA1452.3930625E-829.4867639
GTGCTGG650.005833428429.2395171
GGCGAGC650.00583808929.234748
GCGAGCT650.00583808929.234749
CCTTTAG2650.028.6878281
AAGACAT2500.028.5038725
CATATAG3200.028.1977423
GTTGTTC856.3986436E-427.9449738
GCCATAC700.00838300227.1465458
TACCTAG1056.89427E-527.1465459
TAAGAGT700.00839637627.1376744
Produced by FastQC (version 0.11.5)