##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937141_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 612233 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98185984747637 31.0 31.0 34.0 30.0 34.0 2 32.1046153996926 33.0 31.0 34.0 30.0 34.0 3 32.17798615886436 33.0 31.0 34.0 30.0 34.0 4 35.88631452404558 37.0 35.0 37.0 35.0 37.0 5 35.79036902617141 37.0 35.0 37.0 35.0 37.0 6 35.86043875452646 37.0 35.0 37.0 35.0 37.0 7 35.807821531998435 37.0 35.0 37.0 35.0 37.0 8 35.79005051998177 37.0 35.0 37.0 33.0 37.0 9 37.39398725648569 39.0 37.0 39.0 34.0 39.0 10-11 37.322001590897585 39.0 37.0 39.0 34.0 39.0 12-13 37.27643723876368 39.0 37.0 39.0 34.0 39.0 14-15 38.325131935063936 40.0 38.0 41.0 33.0 41.0 16-17 38.25605855940467 40.0 38.0 41.0 33.0 41.0 18-19 38.471907754074024 40.0 38.0 41.0 34.0 41.0 20-21 38.539221178864906 40.0 38.0 41.0 34.0 41.0 22-23 38.43323783592194 40.0 38.0 41.0 34.0 41.0 24-25 38.29974944179749 40.0 37.5 41.0 34.0 41.0 26-27 38.068032922106454 40.0 37.0 41.0 33.0 41.0 28-29 37.99186339187858 40.0 37.0 41.0 33.0 41.0 30-31 37.888464032484364 40.0 37.0 41.0 33.0 41.0 32-33 37.603618230314275 40.0 36.0 41.0 33.0 41.0 34-35 37.37852663936769 39.5 36.0 41.0 33.0 41.0 36-37 37.128006820932555 39.0 35.0 41.0 32.5 41.0 38-39 36.94102817064745 39.0 35.0 41.0 31.0 41.0 40-41 36.81547221401003 39.0 35.0 41.0 31.0 41.0 42-43 36.46514480598073 39.0 35.0 41.0 30.5 41.0 44-45 36.07858446049135 39.0 35.0 41.0 30.0 41.0 46-47 35.5667368469194 39.0 35.0 40.5 28.0 41.0 48-49 35.05180135667303 39.0 35.0 40.0 24.5 41.0 50-51 34.77387693900852 39.0 35.0 40.0 24.0 41.0 52-53 34.63990996891707 38.5 35.0 40.0 23.5 41.0 54-55 34.398777099568306 38.0 34.0 40.0 22.5 41.0 56-57 34.126647044507564 38.0 34.0 40.0 21.5 41.0 58-59 33.832709932329685 38.0 33.5 40.0 20.0 41.0 60-61 33.46940543877902 38.0 33.0 40.0 19.0 41.0 62-63 33.186346538001054 37.0 33.0 40.0 18.0 41.0 64-65 32.83973421883499 37.0 32.5 39.0 14.5 40.5 66-67 32.402350085669994 36.0 32.0 39.0 12.5 40.0 68-69 32.049201856156074 36.0 31.5 39.0 8.0 40.0 70-71 31.56995130938711 35.5 31.0 38.0 7.5 40.0 72-73 31.03575436149309 35.0 30.5 37.5 4.0 39.5 74-75 30.49369276076265 35.0 30.0 37.0 2.0 39.0 76-77 29.04379133434493 33.0 28.5 35.5 2.0 37.5 78-79 29.769350884385517 34.0 29.0 36.0 2.0 38.0 80-81 29.45825527209412 34.0 29.0 36.0 2.0 37.0 82-83 29.082549454211062 34.0 29.0 35.0 2.0 37.0 84-85 28.693089885713448 34.0 29.0 35.0 2.0 36.5 86-87 28.40792231062357 34.0 29.0 35.0 2.0 36.0 88-89 27.964119052713592 34.0 28.5 35.0 2.0 36.0 90-91 27.453868053502504 33.0 26.5 35.0 2.0 35.0 92-93 26.985307881149822 33.0 26.0 35.0 2.0 35.0 94-95 26.373790697332552 32.5 25.0 34.5 2.0 35.0 96-97 25.684538402862962 32.0 23.5 34.0 2.0 35.0 98-99 24.962888312129532 32.0 19.0 34.0 2.0 35.0 100-101 21.549406026790454 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.257072044369405 1101 2 0.16493696757954268 1101 3 0.11703141111955517 1101 4 0.24653334875835498 1101 5 0.2983231311591794 1101 6 0.2851585367913785 1101 7 0.31518816256296844 1101 8 0.2948058776172786 1101 9 0.3486295559630932 1101 10-11 0.36602063258831663 1101 12-13 0.3710607203143397 1101 14-15 0.6159941549161303 1101 16-17 0.5199543393199662 1101 18-19 0.4825479450113832 1101 20-21 0.5801615076281976 1101 22-23 0.6046810247644601 1101 24-25 0.5875450357220942 1101 26-27 0.26119161956924586 1101 28-29 0.34570085375240467 1101 30-31 0.5595768143447017 1101 32-33 0.7601007821099515 1101 34-35 0.503108520459314 1101 36-37 0.5509458434106804 1101 38-39 0.5893814937400492 1101 40-41 0.5714876907297395 1101 42-43 0.6873747179078791 1101 44-45 0.7135060368224657 1101 46-47 0.9796961992330537 1101 48-49 1.218504164396201 1101 50-51 1.1240058686368926 1101 52-53 1.0707627369088684 1101 54-55 1.0907824371004509 1101 56-57 1.2563882522482714 1101 58-59 0.9907484907250748 1101 60-61 1.081479446802355 1101 62-63 1.1918390179515228 1101 64-65 1.287533744829041 1101 66-67 0.8927246948927134 1101 68-69 0.8707880013199158 1101 70-71 0.7404704976248659 1101 72-73 0.8365303569971587 1101 74-75 0.9559544767052977 1101 76-77 0.7270017581037536 1101 78-79 0.828303893043099 1101 80-81 1.2266112521694765 1101 82-83 0.8277345450963693 1101 84-85 0.7254086296916711 1101 86-87 0.7801076057166689 1101 88-89 0.7298792880151908 1101 90-91 0.8443744699877875 1101 92-93 1.3263963270062789 1101 94-95 1.3600896672747993 1101 96-97 1.2133913111823738 1101 98-99 1.3516870278191604 1101 100-101 1.4656356491680498 1102 1 0.23358719588455656 1102 2 0.3063871840297594 1102 3 0.2900833591715042 1102 4 0.17352469074969434 1102 5 0.22938373721978422 1102 6 0.23158710821994788 1102 7 0.15525309762790585 1102 8 0.15701366982506926 1102 9 0.1610537983873357 1102 10-11 0.31290374947143107 1102 12-13 0.31359318784679857 1102 14-15 0.5083209947429737 1102 16-17 0.6521513090169435 1102 18-19 0.5208704558338937 1102 20-21 0.511774061740752 1102 22-23 0.3963044013878303 1102 24-25 0.4505212261982834 1102 26-27 0.39176521264284503 1102 28-29 0.5544995550510947 1102 30-31 0.5227369875048709 1102 32-33 0.49909428860346594 1102 34-35 0.42179899664979104 1102 36-37 0.6760432460080779 1102 38-39 0.7751607145192736 1102 40-41 0.6860006777427259 1102 42-43 0.7663357568689193 1102 44-45 0.9866986775151076 1102 46-47 0.9041226061594614 1102 48-49 0.7356037314957646 1102 50-51 0.9012461283771529 1102 52-53 0.8387713949175222 1102 54-55 0.9561071124251228 1102 56-57 0.91441855527858 1102 58-59 0.8449584474350331 1102 60-61 0.8651915680144739 1102 62-63 1.163981875094386 1102 64-65 1.0177393725346633 1102 66-67 1.1154086775766956 1102 68-69 0.7879416809735957 1102 70-71 0.7884033980577634 1102 72-73 1.0858052487720506 1102 74-75 1.137004260255079 1102 76-77 0.78917708277908 1102 78-79 0.8894727242119309 1102 80-81 1.26968484524307 1102 82-83 1.0296609520227733 1102 84-85 1.008590447873491 1102 86-87 1.2070015451106109 1102 88-89 1.1780286386645393 1102 90-91 1.3234329115462309 1102 92-93 0.8297296603396127 1102 94-95 0.900392697577832 1102 96-97 1.3156856834767439 1102 98-99 1.2025744737066049 1102 100-101 1.1482222292546318 1103 1 0.34857178756405816 1103 2 0.25763085561241184 1103 3 0.20104527860799948 1103 4 0.1725066410028333 1103 5 0.08647156464659389 1103 6 0.2603126199963057 1103 7 0.3049339252748311 1103 8 0.18125425974362486 1103 9 0.5576254470776334 1103 10-11 0.5450190917562594 1103 12-13 0.518441305830514 1103 14-15 0.515213466677821 1103 16-17 0.6109340516979884 1103 18-19 0.5085713142974697 1103 20-21 0.5378168748598426 1103 22-23 0.4858790216827984 1103 24-25 0.2353275215977959 1103 26-27 0.2627067710844031 1103 28-29 0.3773726565258926 1103 30-31 0.43014949025636184 1103 32-33 0.5032903044520154 1103 34-35 0.6192731326832543 1103 36-37 0.34143505758633097 1103 38-39 0.3087150838787238 1103 40-41 0.3515300636110936 1103 42-43 0.646962545334695 1103 44-45 0.6273683891593933 1103 46-47 0.5186394452525747 1103 48-49 0.7736980524290686 1103 50-51 0.7848995512254859 1103 52-53 0.5245929885780996 1103 54-55 0.5871191089032237 1103 56-57 0.5462559974973757 1103 58-59 0.36324207059153935 1103 60-61 0.697433478646218 1103 62-63 0.7791040410640093 1103 64-65 0.908973138768431 1103 66-67 0.7389779049594836 1103 68-69 0.779866070143747 1103 70-71 0.8957388592067836 1103 72-73 0.9509962018867313 1103 74-75 1.2658838552363711 1103 76-77 1.0754416656538304 1103 78-79 1.1032769284822344 1103 80-81 1.1710578366584343 1103 82-83 1.2726446632268278 1103 84-85 1.168259168982889 1103 86-87 1.1418050890243236 1103 88-89 1.3593515530383051 1103 90-91 1.1117473513437197 1103 92-93 1.3139361318342218 1103 94-95 0.9768564880246693 1103 96-97 1.2299218710180178 1103 98-99 1.245676498800158 1103 100-101 1.4317078114690247 1104 1 0.23205916441061802 1104 2 0.19374943537964384 1104 3 0.16351518237149065 1104 4 0.09847049455948564 1104 5 0.07837207500255516 1104 6 0.0736052137620149 1104 7 0.1738203109400942 1104 8 0.18261886061573307 1104 9 0.16946675328406258 1104 10-11 0.3312601998216991 1104 12-13 0.17005608352916823 1104 14-15 0.09040940478733717 1104 16-17 0.12436572520353195 1104 18-19 0.2201844725744948 1104 20-21 0.18174059057513148 1104 22-23 -0.04223937709025449 1104 24-25 0.0885483845113697 1104 26-27 0.24591598845127294 1104 28-29 0.22462666518980257 1104 30-31 0.2795742383529429 1104 32-33 0.16734962291366884 1104 34-35 0.3463684380275822 1104 36-37 0.2508415157445256 1104 38-39 0.2451658832300012 1104 40-41 0.29713297666481964 1104 42-43 0.24002927741329216 1104 44-45 0.25233653656486865 1104 46-47 0.48959702355041657 1104 48-49 0.33810102168625633 1104 50-51 0.3825220973849568 1104 52-53 0.18749251537357026 1104 54-55 0.009047506652230197 1104 56-57 0.07625816466455859 1104 58-59 0.14826265867974087 1104 60-61 0.29066929739483527 1104 62-63 0.2426968232065505 1104 64-65 0.3184855737831853 1104 66-67 0.2664830668659235 1104 68-69 0.12551494619369663 1104 70-71 0.14447487681085036 1104 72-73 0.4470893472083901 1104 74-75 0.5726713752112182 1104 76-77 0.805305467531884 1104 78-79 0.7573958559488183 1104 80-81 0.8707444309433043 1104 82-83 0.6470132674044571 1104 84-85 0.577386991360914 1104 86-87 0.2848345505904497 1104 88-89 0.5881984533938613 1104 90-91 0.5268706060001556 1104 92-93 0.42748854751117094 1104 94-95 0.5257439691810326 1104 96-97 0.23402758114631084 1104 98-99 0.19277023235290613 1104 100-101 0.43527887430973067 1105 1 0.17537470285610723 1105 2 0.11958931931778238 1105 3 0.11503754608888528 1105 4 0.08792394180538565 1105 5 0.12076689394036322 1105 6 0.1417536288159198 1105 7 0.23859307053842826 1105 8 0.02201446657433337 1105 9 0.2373923371491884 1105 10-11 0.15307584731223756 1105 12-13 0.032410413565870044 1105 14-15 -0.011592757149308852 1105 16-17 0.041861292285204854 1105 18-19 -0.11699704476366435 1105 20-21 -0.009235220388163157 1105 22-23 -6.410611251155274E-4 1105 24-25 0.10564830770572797 1105 26-27 0.3072550142522772 1105 28-29 0.3175413445499444 1105 30-31 0.31839072027516835 1105 32-33 0.19160384959461396 1105 34-35 0.26053694581718645 1105 36-37 0.034948910895337804 1105 38-39 0.2894275060099929 1105 40-41 -0.13460126632547542 1105 42-43 -7.69453871257042E-4 1105 44-45 -0.009949995897372332 1105 46-47 0.15542728307763554 1105 48-49 0.010656516134439187 1105 50-51 -0.06056468351034283 1105 52-53 0.010645149996797443 1105 54-55 0.24895728372622017 1105 56-57 0.18027884529530525 1105 58-59 0.06705728418111079 1105 60-61 -0.04947658591748194 1105 62-63 0.21788196273680427 1105 64-65 -0.105876275620858 1105 66-67 -0.3928376772954252 1105 68-69 -0.09812815409930664 1105 70-71 -0.12097633264098206 1105 72-73 -0.22378661641797137 1105 74-75 -0.08263959282435707 1105 76-77 -0.18273750569992941 1105 78-79 0.009607574024695253 1105 80-81 0.29943845053348284 1105 82-83 0.18894108906364693 1105 84-85 0.30927366036651804 1105 86-87 0.053522738641007095 1105 88-89 -0.28765138683143476 1105 90-91 -0.2519445484171179 1105 92-93 0.02604867874451955 1105 94-95 -0.295298880782461 1105 96-97 -0.11131216530024446 1105 98-99 -0.2235021337350318 1105 100-101 0.22815609906579937 1106 1 0.12474272710032963 1106 2 0.23049921657552375 1106 3 0.09052538702317037 1106 4 0.18042893108766833 1106 5 0.08918658497444198 1106 6 0.16919468136968874 1106 7 0.14338093364730753 1106 8 0.1373962390630652 1106 9 0.34684240736871885 1106 10-11 0.09436400608228723 1106 12-13 0.12844023838662366 1106 14-15 0.3828515846350129 1106 16-17 0.22060694976173778 1106 18-19 0.012557985172023223 1106 20-21 0.3339165277085172 1106 22-23 0.14543403681265232 1106 24-25 0.11446270640482226 1106 26-27 0.13202495290258298 1106 28-29 0.3230803718246875 1106 30-31 0.20600251715594453 1106 32-33 0.033263432712359986 1106 34-35 -0.026028025725416626 1106 36-37 0.06937957835043562 1106 38-39 0.15829715639065256 1106 40-41 0.22383708832009575 1106 42-43 0.22285664561872665 1106 44-45 -0.17030922422171102 1106 46-47 -0.038697375064131734 1106 48-49 -0.016987803475281282 1106 50-51 -0.44433750485708146 1106 52-53 -0.2697894719264795 1106 54-55 -0.3555127436224481 1106 56-57 -0.24378243048264636 1106 58-59 -0.2573338385921886 1106 60-61 -0.5163117178562757 1106 62-63 -0.11032965674727535 1106 64-65 -0.3785405523597163 1106 66-67 -0.38484558623178344 1106 68-69 -0.28152123562787423 1106 70-71 -0.36855560679280686 1106 72-73 -0.3077266711152937 1106 74-75 -0.1221981337364717 1106 76-77 -0.2267633021372113 1106 78-79 -0.2564551431014799 1106 80-81 -0.3070734867863507 1106 82-83 -0.19855059546588194 1106 84-85 -0.18910944259748774 1106 86-87 -0.27205102882148324 1106 88-89 -0.0724701095751712 1106 90-91 -0.08574601193992137 1106 92-93 -0.21243901435918033 1106 94-95 -0.28252077449227286 1106 96-97 -0.5621307771710384 1106 98-99 -0.8575197994900776 1106 100-101 -0.5751876440046395 1107 1 0.14189791501616966 1107 2 0.18147925613675397 1107 3 0.24203141111955517 1107 4 0.05305076169367595 1107 5 0.15441765852236244 1107 6 0.11239734276152546 1107 7 0.2052627894286445 1107 8 0.24726856418444498 1107 9 0.24338079974418747 1107 10-11 0.20870719975249585 1107 12-13 0.2561353471800061 1107 14-15 0.1878349509360362 1107 16-17 0.17623543384733154 1107 18-19 0.1681325221258092 1107 20-21 0.1434202140958547 1107 22-23 0.20755415909281538 1107 24-25 0.34972165263751265 1107 26-27 0.3161667439473561 1107 28-29 0.26159637614046005 1107 30-31 0.20915392877255812 1107 32-33 0.29950376718458216 1107 34-35 0.27110603289713 1107 36-37 0.3648637538584367 1107 38-39 0.3982247773221417 1107 40-41 0.6519727653566036 1107 42-43 0.7809194940272874 1107 44-45 0.7247995691607727 1107 46-47 0.5459524181385333 1107 48-49 0.5624718260877373 1107 50-51 0.45848348057719335 1107 52-53 0.595999055316824 1107 54-55 0.8783570639661207 1107 56-57 0.7315996950343404 1107 58-59 0.765051973312147 1107 60-61 0.9744520836182744 1107 62-63 1.1971375254142131 1107 64-65 1.074237724928544 1107 66-67 0.9903117595693232 1107 68-69 0.9512606381358424 1107 70-71 0.8191894030975 1107 72-73 0.7924756306290028 1107 74-75 1.0843002478495727 1107 76-77 0.8145017581037557 1107 78-79 0.9308785199087701 1107 80-81 1.2813998093834051 1107 82-83 1.1405206147481124 1107 84-85 1.2154086296916695 1107 86-87 1.1837145708908032 1107 88-89 1.1423046611495202 1107 90-91 1.1440013356594285 1107 92-93 1.3655505558620007 1107 94-95 1.2162339458817648 1107 96-97 1.2069485251127219 1107 98-99 1.0421969780679134 1107 100-101 1.292886892949145 1108 1 0.11710154289447772 1108 2 0.02061543365623919 1108 3 -0.006051184750653249 1108 4 0.1541734667524537 1108 5 0.004591567737350033 1108 6 -0.013882761143726441 1108 7 0.13169258734173184 1108 8 0.14246074487391525 1108 9 0.116638405520618 1108 10-11 0.1702094231487905 1108 12-13 -0.00976523838773602 1108 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64.0 7 134.0 8 289.0 9 516.0 10 896.0 11 1481.0 12 2361.0 13 3194.0 14 3968.0 15 5528.0 16 7447.0 17 10588.0 18 8758.0 19 4322.0 20 3441.0 21 3665.0 22 3789.0 23 4270.0 24 4952.0 25 6002.0 26 7064.0 27 8741.0 28 10565.0 29 13204.0 30 15908.0 31 19692.0 32 24321.0 33 31125.0 34 41416.0 35 57013.0 36 82139.0 37 129142.0 38 91492.0 39 4724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.502316044017086 24.78485707516727 17.06422018348624 18.648606697329402 2 13.961705074845309 25.699449196009123 39.21864892942783 21.12019679971774 3 18.196370003528077 29.38232565432712 34.793509649936624 17.62779469220818 4 10.366528918870358 21.247407020237493 43.54915636280483 24.83690769808732 5 9.024505376221144 41.96425217196721 37.36632948566967 11.644912966141975 6 22.569021924659403 44.10298040125246 18.851809686834915 14.476187987253219 7 21.482017467206113 38.24442655002262 24.907184029609642 15.366371953161623 8 23.00758044731336 34.15905382427932 22.276812912730936 20.556552815676383 9 23.47161946513827 19.380856634647266 26.494814882569216 30.65270901764524 10-11 21.198325304530925 29.1722410894995 30.413724269852704 19.215709336116866 12-13 23.865913794258066 29.172226913609688 29.784983821518928 17.17687547061331 14-15 20.91189138775597 29.769140180290837 31.10417112439218 18.21479730756101 16-17 16.047076848193417 32.8006657596046 31.963811163396944 19.18844622880505 18-19 15.887768035646618 32.16027584314443 35.267022958617 16.684933162591957 20-21 17.569449265183284 29.474664238375443 34.81614269205483 18.13974380438644 22-23 17.40282331995312 29.345621894000956 35.12742507830078 18.124129707745144 24-25 18.601686124673908 30.343955058455695 34.613336426140975 16.44102239072942 26-27 17.471314685394642 28.99501554630119 37.34305667968249 16.190613088621674 28-29 15.961664890380698 31.03069503824119 36.26685940390293 16.740780667475182 30-31 17.18372020469799 29.3051996099435 35.63430733120011 17.8767728541584 32-33 17.48828821765294 30.07020535638447 35.905923515690844 16.53558291027174 34-35 18.51296810201345 29.192480640540445 35.437243663768534 16.85730759367757 36-37 18.409149566632145 28.434681189580875 36.00473388632792 17.151435357459054 38-39 16.703953370791563 30.92811629857788 35.2741205769515 17.09380975367905 40-41 16.575469556226693 30.586775820079808 35.75291517688701 17.084839446806495 42-43 16.62463473873509 30.62592183041424 33.99677900407198 18.752664426778693 44-45 17.829078145085287 29.161446704114287 34.24848056213892 18.760994588661507 46-47 19.25384372891931 30.861717227647734 33.39956650349296 16.48487253993999 48-49 18.101751503143493 30.767358632641695 33.30779772531635 17.823092138898463 50-51 16.094044660351177 31.069742902016255 34.48464836160167 18.351564076030897 52-53 17.594036910783966 32.70560391223603 31.914891879398855 17.78546729758115 54-55 17.546243853277392 30.726385192166493 32.33022958381553 19.397141370740577 56-57 18.725932580599057 31.057271780631723 31.711538068889478 18.505257569879742 58-59 19.641802746505615 29.969251877350516 32.17004977684132 18.218895599302552 60-61 18.548639349390054 31.637117369335314 32.96024095696765 16.854002324306975 62-63 17.520736484124626 31.231807594175816 33.355520854091594 17.89193506760796 64-65 17.60473545202562 32.26475867847699 32.317761375162725 17.812744494334673 66-67 18.312638226002985 31.183296255279945 30.634067380786707 19.86999813793036 68-69 17.556714518818815 30.492639240289236 32.48052620489258 19.470120035999365 70-71 19.211335057646338 31.056908045986397 32.152584550275265 17.579172346091998 72-73 19.128991740072816 29.039679337768465 33.87452162820364 17.95680729395508 74-75 17.389621271640046 32.98703271466909 31.498465453511983 18.124880560178884 76-77 17.74928826116854 31.019971154772776 31.974019695116073 19.256720888942606 78-79 17.89877389548098 31.250352199809385 32.04115817181144 18.809715732898198 80-81 18.865149296455613 30.803312199914572 31.35515495617191 18.976383547457907 82-83 17.893777366494792 30.996697847761222 32.691307163865325 18.418217621878657 84-85 17.231043402056045 31.237621376866336 31.712797158419427 19.81853806265819 86-87 17.072624931804892 31.63787499142457 31.667684829161047 19.621815247609494 88-89 17.731172389574223 30.8517597481349 31.142417300617005 20.274650561673873 90-91 17.832268918543683 29.332767097332702 32.45103964196462 20.383924342158995 92-93 18.860793194747753 30.85834968059546 32.179007012036266 18.10185011262052 94-95 18.17747491559586 29.6362659314346 32.50020825404707 19.68605089892247 96-97 17.279448696264343 29.872766637565 32.826272844060526 20.021511822110135 98-99 17.642355635301048 30.817811410991535 30.87669494328479 20.663138010422628 100-101 16.604176688066524 30.076449839643992 32.17271571292885 21.146657759360636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4203.0 1 2650.0 2 1037.5 3 915.0 4 733.0 5 695.5 6 1048.5 7 1822.5 8 2641.0 9 2922.5 10 2369.5 11 1707.0 12 1577.0 13 1476.5 14 1335.5 15 1299.0 16 1311.0 17 1364.5 18 1454.0 19 1611.0 20 1868.0 21 2252.0 22 2624.0 23 2901.0 24 3286.0 25 4396.5 26 7162.5 27 9948.5 28 10226.0 29 10629.0 30 13359.5 31 16039.5 32 19384.5 33 24483.0 34 30528.0 35 33743.0 36 36407.0 37 40595.0 38 37960.5 39 31582.5 40 27085.0 41 23240.0 42 19750.0 43 18069.0 44 17391.0 45 16144.5 46 14543.5 47 18685.0 48 31635.5 49 29065.5 50 12528.5 51 4202.0 52 1754.0 53 1052.0 54 727.5 55 586.5 56 471.5 57 431.5 58 444.5 59 458.5 60 471.0 61 447.5 62 353.5 63 264.5 64 206.0 65 172.0 66 138.0 67 113.5 68 89.5 69 65.0 70 49.0 71 33.5 72 25.0 73 23.5 74 18.5 75 15.5 76 10.5 77 8.5 78 6.0 79 2.0 80 1.0 81 0.5 82 1.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03234062848621358 2 0.00604345077772678 3 1.633365075061292E-4 4 4.900095225183876E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01012686346538001 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.633365075061292E-4 20-21 8.983507912837107E-4 22-23 0.0017150333288143567 24-25 0.009800190450367752 26-27 0.0048184269714308114 28-29 8.16682537530646E-5 30-31 0.002286711105085809 32-33 0.004736758717677747 34-35 0.0 36-37 0.00833016188281259 38-39 0.013801934884267918 40-41 9.800190450367752E-4 42-43 0.0 44-45 0.0 46-47 3.266730150122584E-4 48-49 0.002613384120098067 50-51 0.005635109508961458 52-53 0.0 54-55 0.0026950523738511318 56-57 0.0033483984038756485 58-59 8.16682537530646E-5 60-61 0.001225023806295969 62-63 0.0044100857026654885 64-65 0.0 66-67 0.0017967015825674213 68-69 0.0 70-71 0.0013883603138020982 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0015516968213082274 80-81 0.0018783698363204857 82-83 0.009228512674096299 84-85 0.006288455538985974 86-87 0.0031033936426164547 88-89 8.16682537530646E-5 90-91 4.900095225183876E-4 92-93 0.0 94-95 0.0 96-97 0.0018783698363204857 98-99 0.001225023806295969 100-101 2.450047612591938E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 612233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.531995171772838 #Duplication Level Percentage of deduplicated Percentage of total 1 70.57901821394019 10.96232970127386 2 9.946157405459974 3.08967337598594 3 4.1191688049467885 1.9193672997045244 4 2.3629748033483366 1.4680685294650893 5 1.5879358936608758 1.2331906316712755 6 1.168342236991545 1.0888011590358573 7 0.9780002523871619 1.063320663864901 8 0.7161485719093088 0.889857292893392 9 0.6677743658772557 0.9334681403975285 >10 5.835401505910066 19.439004431319447 >50 1.0968325411180753 12.07857139357075 >100 0.8454969923863206 25.396376869590497 >500 0.06309679047659109 6.481192617843207 >1k 0.029445168889075845 9.48086757819327 >5k 0.0042064526984394056 4.4759103151904585 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9903 1.6175214338331974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6507 1.0628306543423827 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5506 0.8993308103287474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5487 0.8962274166861308 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.7439977916904185 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4219 0.6891167251683591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.6299889094511404 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3824 0.6245988047034381 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3078 0.5027497701038657 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2754 0.44982874167187986 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2751 0.44933873214936143 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2657 0.43398510044378535 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2543 0.41536473858808654 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2540 0.4148747290655681 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2323 0.37943070693673814 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2101 0.3431700022703774 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1809 0.29547574207858773 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1641 0.268035208817558 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1639 0.2677085358025457 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1570 0.25643831678462287 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1442 0.2355312438238383 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1408 0.22997780256862993 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1356 0.2214843041783112 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1180 0.19273707885723246 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1168 0.1907770407671589 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1159 0.18930701219960375 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1108 0.18097685031679117 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1108 0.18097685031679117 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1098 0.17934348524172986 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1063 0.17362670747901535 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1056 0.17248335192647243 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1038 0.1695432947913621 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 974 0.15908975831096986 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 937 0.15304630753324305 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 885 0.14455280914292434 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 879 0.14357279009788756 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 873 0.1425927710528508 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 869 0.14193942502282628 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 854 0.13948937741023434 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 841 0.13736600281265465 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 828 0.13524262821507496 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 817 0.13344592663250757 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 796 0.13001585997487886 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 790 0.12903584092984208 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 772 0.12609578379473174 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 769 0.12560577427221337 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 746 0.12184903459957239 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 745 0.12168569809206625 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 718 0.11727561238940078 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 713 0.11645892985187012 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 700 0.11433555525429044 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 693 0.11319219970174754 No Hit AACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCA 689 0.11253885367172303 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 680 0.11106882510416786 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 673 0.10992546955162494 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 665 0.10861877749157592 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 658 0.10747542193903302 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 655 0.10698541241651463 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 652 0.10649540289399623 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 643 0.10502537432644109 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 639 0.10437202829641656 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 638 0.10420869178891043 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 638 0.10420869178891043 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 628 0.10257532671384914 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 614 0.10028861560876332 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.16682537530646E-5 0.0 14-15 0.0 0.0 0.0 1.633365075061292E-4 0.0 16-17 0.0 0.0 0.0 2.450047612591938E-4 0.0 18-19 0.0 0.0 0.0 6.533460300245168E-4 0.0 20-21 0.0 0.0 0.0 0.0011433555525429044 0.0 22-23 0.0 0.0 0.0 0.001878369836320486 0.0 24-25 0.0 0.0 0.0 0.0025317158663450026 0.0 26-27 0.0 0.0 0.0 0.003756739672640972 0.0 28-29 0.0 0.0 0.0 0.008411830136565653 0.0 30-31 0.0 0.0 0.0 0.01633365075061292 0.0 32-33 0.0 0.0 0.0 0.025807168185968413 0.0 34-35 0.0 0.0 0.0 0.037975737995175035 0.0 36-37 0.0 0.0 0.0 0.049654298281863275 0.0 38-39 0.0 0.0 0.0 0.0721947363177091 0.0 40-41 0.0 0.0 0.0 0.09677688069738155 0.0 42-43 0.0 0.0 0.0 0.12225737586833771 0.0 44-45 0.0 0.0 0.0 0.14275610756035692 0.0 46-47 0.0 0.0 0.0 0.1739533804940276 0.0 48-49 0.0 0.0 0.0 0.1951871264698244 0.0 50-51 0.0 0.0 0.0 0.21462417086305377 0.0 52-53 0.0 1.633365075061292E-4 0.0 0.2594600421734863 0.0 54-55 0.0 1.633365075061292E-4 0.0 0.33573819117884857 0.0 56-57 0.0 1.633365075061292E-4 0.0 0.46191564322733336 0.0 58-59 0.0 1.633365075061292E-4 0.0 0.6147986142530704 0.0 60-61 0.0 1.633365075061292E-4 0.0 0.80026721852628 0.0 62-63 0.0 1.633365075061292E-4 0.0 0.914031096004299 0.0 64-65 0.0 1.633365075061292E-4 0.0 1.0432302734416472 0.0 66-67 0.0 1.633365075061292E-4 0.0 1.2686346538001056 0.0 68-69 0.0 1.633365075061292E-4 0.0 1.601677792605103 0.0 70-71 0.0 1.633365075061292E-4 0.0 1.9229607028696591 0.0 72-73 0.0 1.633365075061292E-4 0.0 2.241466892506611 0.0 74-75 0.0 3.266730150122584E-4 0.0 2.5818601741493845 0.0 76-77 0.0 3.266730150122584E-4 0.0 2.8587155543722735 0.0 78-79 0.0 3.266730150122584E-4 0.0 3.1331208869825704 0.0 80-81 0.0 3.266730150122584E-4 0.0 3.399277725963808 0.0 82-83 0.0 3.266730150122584E-4 0.0 3.713128825136835 0.0 84-85 0.0 3.266730150122584E-4 0.0 4.239235715814077 0.0 86-87 0.0 3.266730150122584E-4 0.0 4.900013556930123 0.0 88-89 0.0 3.266730150122584E-4 0.0 5.651933169234589 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGC 25 0.005017459 57.007744 6 GAGCATA 25 0.005017459 57.007744 6 ATGTATT 70 4.298039E-6 40.72647 1 ATGGGCG 80 1.0757834E-5 35.62984 5 AAAGACA 175 0.0 35.27898 4 ATACTCA 155 3.6379788E-11 33.714256 6 GGACAAT 255 0.0 31.670967 6 GACAATG 265 0.0 30.475838 7 GCAGAGA 360 0.0 30.343906 94-95 CGCAGAA 145 2.3930625E-8 29.486763 9 GTGCTGG 65 0.0058334284 29.239517 1 GGCGAGC 65 0.005838089 29.23474 8 GCGAGCT 65 0.005838089 29.23474 9 CCTTTAG 265 0.0 28.687828 1 AAGACAT 250 0.0 28.503872 5 CATATAG 320 0.0 28.197742 3 GTTGTTC 85 6.3986436E-4 27.944973 8 GCCATAC 70 0.008383002 27.146545 8 TACCTAG 105 6.89427E-5 27.146545 9 TAAGAGT 70 0.008396376 27.137674 4 >>END_MODULE