##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 627337 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.779300439795517 33.0 31.0 34.0 30.0 34.0 2 31.8666793127139 33.0 31.0 34.0 30.0 34.0 3 31.857645890486292 33.0 31.0 34.0 30.0 34.0 4 35.47535056915183 37.0 35.0 37.0 33.0 37.0 5 35.508783955035334 37.0 35.0 37.0 33.0 37.0 6 35.61268185998913 37.0 35.0 37.0 33.0 37.0 7 35.57784731332601 37.0 35.0 37.0 33.0 37.0 8 35.55326562915945 37.0 35.0 37.0 33.0 37.0 9 37.13661875515074 39.0 37.0 39.0 33.0 39.0 10-11 37.03419374275708 39.0 37.0 39.0 33.0 39.0 12-13 36.9991288573765 39.0 37.0 39.0 33.0 39.0 14-15 38.0489896180203 40.0 38.0 41.0 33.0 41.0 16-17 37.997106021165656 40.0 38.0 41.0 33.0 41.0 18-19 38.07439940574205 40.0 38.0 41.0 33.0 41.0 20-21 38.04149922609379 40.0 38.0 41.0 33.0 41.0 22-23 37.926207923333074 40.0 37.5 41.0 33.0 41.0 24-25 37.859055818483526 40.0 37.0 41.0 33.0 41.0 26-27 37.83495633128605 40.0 37.0 41.0 33.0 41.0 28-29 37.784193344247186 40.0 37.0 41.0 33.0 41.0 30-31 37.59449944766529 40.0 37.0 41.0 33.0 41.0 32-33 37.32479193798548 39.5 36.0 41.0 32.5 41.0 34-35 37.122482812268366 39.0 35.0 41.0 32.0 41.0 36-37 36.88402007214623 39.0 35.0 41.0 31.0 41.0 38-39 36.68625635025512 39.0 35.0 41.0 31.0 41.0 40-41 36.599660150764265 39.0 35.0 41.0 31.0 41.0 42-43 36.314613198328814 39.0 35.0 41.0 30.0 41.0 44-45 35.96088466007903 39.0 35.0 41.0 30.0 41.0 46-47 35.46229937019497 39.0 35.0 40.5 27.5 41.0 48-49 35.040191316628864 39.0 35.0 40.5 24.5 41.0 50-51 34.27387672016795 38.0 34.0 40.0 23.5 40.5 52-53 34.513413842958414 38.5 34.5 40.0 24.0 40.5 54-55 34.95311610824804 39.0 35.0 40.5 24.0 41.0 56-57 34.85332046412056 39.0 35.0 41.0 23.0 41.0 58-59 34.68814767820167 39.0 35.0 41.0 23.0 41.0 60-61 34.38706309368011 39.0 34.5 40.5 22.0 41.0 62-63 34.14290405316441 38.0 34.0 40.0 22.0 41.0 64-65 33.861920307585876 38.0 34.0 40.0 20.0 41.0 66-67 33.45423034190554 37.0 34.0 40.0 18.0 41.0 68-69 33.01680436511795 37.0 33.0 39.0 14.5 41.0 70-71 32.55226696337056 36.0 33.0 39.0 10.0 40.5 72-73 32.138827296971165 36.0 32.0 38.5 7.5 40.0 74-75 31.730893443237047 35.0 32.0 37.5 4.5 40.0 76-77 31.297109846860618 35.0 32.0 37.0 2.0 39.0 78-79 30.797519515029403 35.0 31.0 36.5 2.0 39.0 80-81 30.28589976360393 35.0 31.0 36.0 2.0 38.5 82-83 29.844074237610727 35.0 30.5 36.0 2.0 37.0 84-85 29.43874345048993 34.0 30.0 35.0 2.0 37.0 86-87 29.182832353264672 34.0 30.0 35.0 2.0 36.0 88-89 28.759928873954507 34.0 29.0 35.0 2.0 36.0 90-91 28.36834588108146 34.0 29.0 35.0 2.0 36.0 92-93 28.015318720241275 34.0 29.0 35.0 2.0 35.5 94-95 27.616160851344652 34.0 27.5 35.0 2.0 35.0 96-97 27.17504626699844 34.0 26.5 35.0 2.0 35.0 98-99 26.748909278426108 34.0 25.5 35.0 2.0 35.0 100-101 24.67116079555327 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3513216240979169 1101 2 0.4279584738052442 1101 3 0.5884390592110584 1101 4 0.5124418821953682 1101 5 0.3118582053911183 1101 6 0.30338868117608087 1101 7 0.3672144294840223 1101 8 0.4325423747083974 1101 9 0.5564515853887926 1101 10-11 0.6521745417563949 1101 12-13 0.6264725514314264 1101 14-15 0.8324856588973759 1101 16-17 0.8689961653226419 1101 18-19 0.8109341998848407 1101 20-21 0.7176755930006848 1101 22-23 0.7331872321747568 1101 24-25 0.8176728925004753 1101 26-27 0.9008819742834291 1101 28-29 0.7434741716804112 1101 30-31 0.8046906699154235 1101 32-33 0.7908066916950673 1101 34-35 0.76214954475968 1101 36-37 0.8296337576846398 1101 38-39 0.7030417422339852 1101 40-41 0.6275519133958412 1101 42-43 0.8053048210475353 1101 44-45 0.7434612411786929 1101 46-47 0.7529696964697905 1101 48-49 0.8051472225168013 1101 50-51 0.8552462289770801 1101 52-53 0.8568550595256568 1101 54-55 0.8910772075845728 1101 56-57 1.071392559672944 1101 58-59 0.9566783512750021 1101 60-61 1.0694422627284226 1101 62-63 0.8459646488017327 1101 64-65 0.8802154827199118 1101 66-67 0.8849373695361038 1101 68-69 0.8309261539616628 1101 70-71 0.972842845253588 1101 72-73 0.7971175655094598 1101 74-75 0.9128682759038185 1101 76-77 0.7878997804293242 1101 78-79 0.9765585946577886 1101 80-81 1.1363378253187335 1101 82-83 1.1288456039189398 1101 84-85 1.0808907277163904 1101 86-87 1.070865703719047 1101 88-89 1.4059518180474946 1101 90-91 1.3799742772354655 1101 92-93 1.5349992562793204 1101 94-95 1.6244351996896675 1101 96-97 1.7547241385844998 1101 98-99 1.4125884894045946 1101 100-101 2.0371497035997486 1102 1 0.24272166384355032 1102 2 0.14310652626947373 1102 3 0.05137377701710122 1102 4 0.15029710874544833 1102 5 0.1585866433879346 1102 6 0.14058313666097888 1102 7 0.18056150818036798 1102 8 -0.057836699218476895 1102 9 0.14442853292456448 1102 10-11 0.19165933905369315 1102 12-13 0.10963406772713569 1102 14-15 0.0930058298035803 1102 16-17 0.35543421182502755 1102 18-19 0.2730441959102805 1102 20-21 0.08544064665728257 1102 22-23 0.19424396905869656 1102 24-25 0.09595811706327595 1102 26-27 0.07027535663637252 1102 28-29 -0.04022887879653325 1102 30-31 0.1056172398677333 1102 32-33 0.1370855072753514 1102 34-35 0.1344021282254957 1102 36-37 0.05981186778002723 1102 38-39 -0.05696918781371352 1102 40-41 -0.04950368080129408 1102 42-43 0.18331431031621293 1102 44-45 0.3944188624028584 1102 46-47 0.5011600283457867 1102 48-49 0.3278916581288911 1102 50-51 0.3747576061630937 1102 52-53 0.5165393301933818 1102 54-55 0.3143296420042887 1102 56-57 0.48025707676356433 1102 58-59 0.4703520893513158 1102 60-61 0.6371079523150698 1102 62-63 0.42625578552669907 1102 64-65 0.41592196125726844 1102 66-67 0.2768168409192526 1102 68-69 0.38642843933526905 1102 70-71 0.4001207665572437 1102 72-73 0.31519313188465503 1102 74-75 0.5712508772551743 1102 76-77 0.7980200109539659 1102 78-79 0.551518849029808 1102 80-81 0.6793425947940932 1102 82-83 0.8009093956518498 1102 84-85 0.504396540514481 1102 86-87 0.5275678102373327 1102 88-89 0.8059182826738862 1102 90-91 0.6893207100653491 1102 92-93 0.8864251306831363 1102 94-95 0.6962381607389538 1102 96-97 0.4883377613984905 1102 98-99 0.7119488332042749 1102 100-101 0.6393642803088113 1103 1 0.14568395327693295 1103 2 0.038660699316945824 1103 3 0.14540373490378045 1103 4 0.0614403897561715 1103 5 0.033388040784565476 1103 6 0.12077791078042566 1103 7 0.22660620573013546 1103 8 0.15894880996988547 1103 9 0.01619346401185595 1103 10-11 0.17305530000315628 1103 12-13 0.19724533304106018 1103 14-15 0.28836618585258833 1103 16-17 0.23558232629669362 1103 18-19 0.11220240800828662 1103 20-21 0.17429138364043695 1103 22-23 0.0897000372057093 1103 24-25 0.3067388885027853 1103 26-27 0.47339796383391786 1103 28-29 0.32976418207636016 1103 30-31 0.3994605591632876 1103 32-33 0.17210177833201357 1103 34-35 0.22495055854709278 1103 36-37 0.19292705728997817 1103 38-39 0.025989273135749613 1103 40-41 0.00941549033193212 1103 42-43 -0.18770287000538133 1103 44-45 -0.04169690137968729 1103 46-47 0.05419529825390157 1103 48-49 0.22152680361127608 1103 50-51 0.1748473362549774 1103 52-53 0.2268606835624496 1103 54-55 0.28083878250964744 1103 56-57 0.31713375172527947 1103 58-59 0.20504652847841953 1103 60-61 0.46862714259071936 1103 62-63 0.44665681921735967 1103 64-65 0.2701572215941823 1103 66-67 0.07152635470179547 1103 68-69 0.3444323635312898 1103 70-71 0.31712641357102456 1103 72-73 0.003457632267505062 1103 74-75 0.09500063797492686 1103 76-77 0.25615075367985085 1103 78-79 0.5353626468858543 1103 80-81 0.3212262088829121 1103 82-83 0.37288010774985736 1103 84-85 0.13157279434296854 1103 86-87 0.10621922677638551 1103 88-89 0.26380854875352 1103 90-91 -0.009756864590084291 1103 92-93 -0.04492536701977201 1103 94-95 0.2286247419028129 1103 96-97 0.1862715764827172 1103 98-99 0.2832100793757881 1103 100-101 0.7041434246426554 1104 1 0.16563523951120374 1104 2 0.11857944911813689 1104 3 -0.07671876749328277 1104 4 0.11322830310746923 1104 5 0.08603935110306793 1104 6 0.04269274348590102 1104 7 -0.21258111051399453 1104 8 0.013616379416539814 1104 9 0.10321933411150752 1104 10-11 -0.06313943691069568 1104 12-13 0.001513240575796715 1104 14-15 -0.19267202627950297 1104 16-17 0.052826181409599826 1104 18-19 -0.13028944652295849 1104 20-21 -0.014232480197314601 1104 22-23 -0.0826430858374394 1104 24-25 -0.03286467596343101 1104 26-27 -0.08515398312624001 1104 28-29 -0.03588216143748468 1104 30-31 0.13921794461550974 1104 32-33 0.08434997315072934 1104 34-35 0.1955019056735665 1104 36-37 0.1331311370678634 1104 38-39 0.32020996648895306 1104 40-41 0.1638749356675504 1104 42-43 -0.040301694876660576 1104 44-45 0.20449165542956393 1104 46-47 0.24499580356834372 1104 48-49 0.4703805305324451 1104 50-51 0.3097144867268895 1104 52-53 0.4538886439916041 1104 54-55 0.4803232436363274 1104 56-57 0.5858649217190504 1104 58-59 0.6552626049299519 1104 60-61 0.46002384623198367 1104 62-63 0.4328117944287726 1104 64-65 0.41526357846795037 1104 66-67 0.3794921005631693 1104 68-69 0.39579880283675806 1104 70-71 0.4146561894059104 1104 72-73 0.5186681875226427 1104 74-75 0.5260890634569506 1104 76-77 0.4218979783130159 1104 78-79 0.6644268608992441 1104 80-81 0.6158411111145661 1104 82-83 0.5035336538120845 1104 84-85 0.7636486733038019 1104 86-87 0.9794873206230896 1104 88-89 0.7461236795581563 1104 90-91 0.3661729912226228 1104 92-93 0.44025487845761546 1104 94-95 0.7084553343294608 1104 96-97 0.7750333289059057 1104 98-99 0.6793241113941839 1104 100-101 0.32427943238596413 1105 1 -0.0513917082494757 1105 2 0.28736524719970546 1105 3 0.013837439807801388 1105 4 0.014214259908236215 1105 5 0.10433518989956525 1105 6 0.19774156689353362 1105 7 0.19556877562222752 1105 8 0.263711265897804 1105 9 0.06332388176177517 1105 10-11 0.18348346696067352 1105 12-13 0.14268275958759347 1105 14-15 0.26578926003613645 1105 16-17 0.26809772926689135 1105 18-19 0.0016415796312045927 1105 20-21 0.10678875712239488 1105 22-23 0.22182391091916287 1105 24-25 -0.018340574797186093 1105 26-27 0.18375646128753687 1105 28-29 0.05470208050579828 1105 30-31 -0.05960805082994369 1105 32-33 -0.13130112063162613 1105 34-35 -0.05520542991403943 1105 36-37 0.07712654801258623 1105 38-39 0.17958604474442552 1105 40-41 0.07144472036149807 1105 42-43 0.27631939752551204 1105 44-45 0.3912756937982067 1105 46-47 0.41901976671788077 1105 48-49 0.49460677440796275 1105 50-51 0.1944705422096078 1105 52-53 0.1808743592631501 1105 54-55 -0.032018468460826455 1105 56-57 0.05919966397286203 1105 58-59 0.23882956028154467 1105 60-61 0.1982816441755304 1105 62-63 0.1866482273871597 1105 64-65 0.2793667286991308 1105 66-67 0.36965841068669647 1105 68-69 0.42345605561434496 1105 70-71 0.11178501074329006 1105 72-73 0.5333799051404711 1105 74-75 0.4764289295807558 1105 76-77 0.537918266767921 1105 78-79 0.39555954670422366 1105 80-81 0.1690228273522294 1105 82-83 0.048656488675103304 1105 84-85 0.1233827323749459 1105 86-87 0.23287304279547172 1105 88-89 0.4507765675576074 1105 90-91 0.36978946006535196 1105 92-93 0.7676024562645303 1105 94-95 0.678251242134305 1105 96-97 0.5237847090729062 1105 98-99 0.3219233971577644 1105 100-101 0.27481122798322843 1106 1 0.30479884113747957 1106 2 0.2332358030968429 1106 3 0.34581147141834734 1106 4 0.2718343365837086 1106 5 0.20521135178606897 1106 6 0.32053307834060263 1106 7 0.27030927256605963 1106 8 0.24156308694015394 1106 9 0.23795497149377098 1106 10-11 0.285397188975935 1106 12-13 0.47620069875356563 1106 14-15 0.45467719450031296 1106 16-17 0.5364618654020177 1106 18-19 0.5949021858014092 1106 20-21 0.3872182030336475 1106 22-23 0.346020310427285 1106 24-25 0.42666986861848244 1106 26-27 0.3778740152677855 1106 28-29 0.2837711698037779 1106 30-31 0.3923906652176541 1106 32-33 0.6644329022986852 1106 34-35 0.4652293055194292 1106 36-37 0.5010455866758292 1106 38-39 0.1826546457788183 1106 40-41 0.3956958234755348 1106 42-43 0.5362485540953799 1106 44-45 0.5995449802100126 1106 46-47 0.6730581134935392 1106 48-49 0.7911206627945191 1106 50-51 1.044320446365269 1106 52-53 1.0069711439569886 1106 54-55 1.1791928321286989 1106 56-57 1.1986972497806718 1106 58-59 1.3816771768318716 1106 60-61 1.348430609780074 1106 62-63 1.346517255200105 1106 64-65 0.9836270545698653 1106 66-67 1.0173762304993446 1106 68-69 1.015897237935583 1106 70-71 0.9764354049709283 1106 72-73 1.0991756843729945 1106 74-75 1.1817934997279593 1106 76-77 0.895517094935304 1106 78-79 0.9278018972413129 1106 80-81 0.8973052697552077 1106 82-83 1.0212097455740867 1106 84-85 0.7831545595658049 1106 86-87 0.9356403219014062 1106 88-89 0.9379325225650206 1106 90-91 0.8944298177636405 1106 92-93 1.0114980311496993 1106 94-95 1.3256729391215352 1106 96-97 1.4700045090656744 1106 98-99 1.6176314245728598 1106 100-101 2.150657777587192 1107 1 0.1670506515736072 1107 2 0.03085575939830676 1107 3 0.16532124634225553 1107 4 0.04231206273318122 1107 5 0.060302517621067864 1107 6 0.2124102072131251 1107 7 0.17723520449938235 1107 8 -0.06235165320620695 1107 9 -0.038776988547830626 1107 10-11 0.09977858751994972 1107 12-13 0.20213310913817395 1107 14-15 0.07493642796308819 1107 16-17 0.3333531151992659 1107 18-19 0.29500546891641477 1107 20-21 0.5036490438965444 1107 22-23 0.518429168445202 1107 24-25 0.4925695051000858 1107 26-27 0.420955954795879 1107 28-29 0.06380198776788149 1107 30-31 0.2644841877204911 1107 32-33 0.13376878948394477 1107 34-35 0.2837694595138416 1107 36-37 0.17385040305610744 1107 38-39 0.029399677216964903 1107 40-41 0.1952097011005378 1107 42-43 0.39841687933461856 1107 44-45 0.007278333261751868 1107 46-47 0.20686842558505703 1107 48-49 0.2779300016871744 1107 50-51 0.24303310462934746 1107 52-53 0.05394826424246446 1107 54-55 0.15010897482637375 1107 56-57 0.14947199240774012 1107 58-59 0.40352406021020926 1107 60-61 0.282847024400958 1107 62-63 0.46063078552669623 1107 64-65 0.643126715858493 1107 66-67 0.48880878877201184 1107 68-69 0.209271614181894 1107 70-71 0.37984661011552845 1107 72-73 0.3924985383263717 1107 74-75 0.5962748419790955 1107 76-77 0.40348396800917996 1107 78-79 0.42621210056354997 1107 80-81 0.3374714291499181 1107 82-83 0.39310648154141603 1107 84-85 0.3532701984899411 1107 86-87 0.4563446507281306 1107 88-89 0.43328120445302787 1107 90-91 0.40257320239664196 1107 92-93 0.6390133208671855 1107 94-95 0.7313704460150241 1107 96-97 0.4022133365518634 1107 98-99 0.3782304666398595 1107 100-101 0.728393229695314 1108 1 0.06438207156886833 1108 2 0.12175906561138916 1108 3 -0.021071233712511628 1108 4 -0.04915076321449163 1108 5 -0.051331543307341576 1108 6 0.05320160947928798 1108 7 -0.0927999903890182 1108 8 -0.07532373426854377 1108 9 -0.2446451437564079 1108 10-11 -0.22434790343557864 1108 12-13 0.11628083990166971 1108 14-15 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2316 52-53 -1.9406988734490085 2316 54-55 -1.8649491245520053 2316 56-57 -1.7680614248920676 2316 58-59 -1.895184955350679 2316 60-61 -2.161013943380297 2316 62-63 -1.9731440489103917 2316 64-65 -2.588900058610278 2316 66-67 -2.1613557584184946 2316 68-69 -2.065527272585257 2316 70-71 -1.9340576274824919 2316 72-73 -2.455848879704746 2316 74-75 -2.8640916310229727 2316 76-77 -2.9574228698407374 2316 78-79 -2.793514263552975 2316 80-81 -2.9933395244112084 2316 82-83 -3.3370665977256344 2316 84-85 -2.97059828564446 2316 86-87 -3.285656527511012 2316 88-89 -3.2174623050744593 2316 90-91 -3.4524373611267016 2316 92-93 -3.586360465343354 2316 94-95 -3.9498193723736286 2316 96-97 -4.290703008469059 2316 98-99 -4.7056784432857945 2316 100-101 -5.015669867042181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1109.0 3 318.0 4 310.0 5 307.0 6 354.0 7 450.0 8 631.0 9 764.0 10 1045.0 11 1547.0 12 2315.0 13 3293.0 14 3791.0 15 4994.0 16 6983.0 17 9861.0 18 8350.0 19 3699.0 20 3489.0 21 3489.0 22 3731.0 23 4179.0 24 4795.0 25 5559.0 26 6340.0 27 7620.0 28 9231.0 29 10898.0 30 13275.0 31 16626.0 32 20604.0 33 27253.0 34 36520.0 35 53623.0 36 74365.0 37 118535.0 38 133190.0 39 23894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.3462165256521 24.391426229665043 16.16702970075998 19.095327543922874 2 13.829368274533751 25.114040947915058 39.5983972882091 21.458193489342094 3 17.778886764806703 29.78767504412475 34.39466580782176 18.038772383246783 4 10.07211339121116 20.86025757972022 43.872594359782354 25.195034669286265 5 9.132652491412362 41.792642631383295 37.21857833697367 11.856126540230674 6 22.3901057251147 44.02553361260722 19.029563135846796 14.55479752643128 7 21.391501007987344 38.30750631587006 24.92663383264858 15.374358843494015 8 22.614118807047436 34.21354508193452 22.448670269200885 20.72366584181716 9 23.554269433672108 19.587693004691616 26.27990485959452 30.578132702041756 10-11 20.962897146020204 29.21377171671088 30.447103827054345 19.37622731021457 12-13 23.584164496536435 29.62516899099911 29.43613835128593 17.354528161178532 14-15 20.873509205957276 30.207311490419205 30.582871161260172 18.336308142363354 16-17 16.129870875545464 33.24452305611555 31.355742975986935 19.269863092352054 18-19 15.959980221392797 32.68837528312119 34.33614061951702 17.015503875968992 20-21 17.753936405047785 30.28551696418318 33.70825943269832 18.252287198070714 22-23 17.54710332115089 30.169246665773713 33.98056549498351 18.303084518091886 24-25 18.48933278599014 31.200395362462086 33.486961408319345 16.82331044322842 26-27 17.439130143690164 29.965551981279155 36.07509381147162 16.520224063559066 28-29 16.082997500382596 31.732387899811254 35.04071443146457 17.14390016834158 30-31 17.22952777055991 30.168451827750953 34.47045357810684 18.131566823582297 32-33 17.382938598658694 30.805086569562523 34.8165707262406 16.99540410553819 34-35 18.365129782917993 30.198749397215835 34.20466365639908 17.231457163467095 36-37 18.323404648760494 29.404820925001633 34.75953560218522 17.512238824052655 38-39 16.641360703625327 31.776159790219545 34.11506840580403 17.467411100351097 40-41 16.508886917472303 31.498082651241795 34.83038776595194 17.16264266533397 42-43 16.574527507842987 31.43340156094575 32.905794883566706 19.086276047644553 44-45 17.600286932610686 30.243892719085004 33.19188618339776 18.963934164906547 46-47 19.05175601917976 31.591288767598446 32.53122768312589 16.8257275300959 48-49 17.835512257795074 31.407197584067852 32.657644912491 18.09964524564607 50-51 15.872872912764516 31.72047981508787 33.92890447535169 18.47774279679592 52-53 17.412100594923068 33.426642096067646 31.205484387254746 17.955772921754544 54-55 17.27838167668766 31.344651754876118 31.794619990068018 19.582346578368206 56-57 18.506084098129897 31.787718937462838 31.016306626430158 18.68989033797711 58-59 19.38189261312733 30.603760230319686 31.546288709425447 18.46805844712754 60-61 18.46527598442895 32.112979382067785 32.35687482265773 17.064869810845536 62-63 17.29385217121682 32.12949744654157 32.5673356592243 18.009314723017315 64-65 17.426076227625998 32.84742917672602 31.82043889985709 17.906055695790894 66-67 18.05617668512119 31.794234057563763 30.168482225065468 19.98110703224958 68-69 17.43168937042908 31.028112052715677 31.976024699312028 19.564173877543208 70-71 18.964135419831624 31.722525393411473 31.49492769745934 17.818411489297564 72-73 18.885815851559013 29.902282726519157 33.13308997003124 18.078811451890584 74-75 17.237110608041323 33.53003539202245 30.949127953320794 18.28372604661544 76-77 17.608897718014656 31.59512576986912 31.548889778178847 19.247086733937376 78-79 17.81532957159917 31.821066242767376 31.546589327368128 18.817014858265317 80-81 18.73877337451225 31.39594613134075 30.833936872465532 19.031343621681465 82-83 17.771504332636805 31.663925467007143 32.24750999946585 18.317060200890207 84-85 17.490434588527883 31.665178713771002 31.228358256544336 19.61602844115678 86-87 17.41220985762554 32.27335130885962 31.11525331300855 19.19918552050629 88-89 18.000920184004908 31.412801402124398 30.592928617164382 19.993349796706315 90-91 17.900635569401132 29.796890486073845 32.0972514473935 20.205222497131526 92-93 19.17188468036892 31.355285536248083 31.7982950989077 17.67453468447529 94-95 18.46663759823549 29.87012469400428 32.33578661327003 19.327451094490204 96-97 17.310692327252987 30.21230327490536 32.76651103764989 19.710493360191762 98-99 17.93166353852557 31.293104960429147 30.650547039322092 20.124684461723195 100-101 17.23933299620491 30.274238549730963 32.0243019607155 20.462126493348627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3668.0 1 2263.5 2 859.0 3 786.0 4 661.0 5 758.0 6 1167.0 7 2018.0 8 2910.5 9 3060.5 10 2376.5 11 1775.5 12 1718.5 13 1628.0 14 1510.5 15 1427.5 16 1379.5 17 1434.5 18 1493.0 19 1628.0 20 1895.5 21 2257.5 22 2605.5 23 2928.5 24 3236.0 25 4335.5 26 7216.5 27 10081.5 28 10284.5 29 10706.0 30 13719.5 31 16490.0 32 19933.5 33 25301.5 34 31719.5 35 34954.0 36 37379.5 37 41933.5 38 39293.0 39 32131.0 40 27277.5 41 23907.5 42 20634.5 43 18617.5 44 17848.5 45 16549.0 46 15030.5 47 18946.5 48 31402.5 49 29149.0 50 12959.5 51 4421.0 52 1891.5 53 1196.5 54 837.0 55 704.0 56 572.5 57 502.0 58 484.5 59 499.5 60 523.5 61 488.5 62 375.0 63 262.0 64 227.5 65 194.5 66 154.0 67 130.0 68 99.0 69 77.5 70 57.5 71 50.5 72 45.5 73 30.0 74 22.0 75 23.5 76 18.5 77 11.0 78 7.5 79 5.0 80 3.0 81 2.0 82 2.0 83 1.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03427185069587797 2 0.023910593508752076 3 0.021997746028051907 4 0.04287966435902872 5 0.08990383159290781 6 0.11349561718820986 7 0.05499436507012977 8 0.08862859993910768 9 0.07555747548765655 10-11 0.07300701218005633 12-13 0.07244909833151879 14-15 0.0647180064303556 16-17 0.05499436507012977 18-19 0.06328337081983049 20-21 0.058820060031530105 22-23 0.03267781112862783 24-25 0.010281555208763392 26-27 0.0023910593508752074 28-29 0.006535562225725567 30-31 0.005738542442100498 32-33 0.006057350355550525 34-35 0.0062964562906380455 36-37 0.004782118701750415 38-39 0.0023113573725127004 40-41 0.006137052333913032 42-43 0.003985098918125345 44-45 0.001912847480700166 46-47 0.0014346356105251245 48-49 2.3910593508752074E-4 50-51 0.001912847480700166 52-53 0.004622714745025401 54-55 0.011237978949113476 56-57 0.007332582009350636 58-59 0.005579138485375484 60-61 0.0030286751777752626 62-63 0.008049899814613197 64-65 0.002948973199412756 66-67 0.019048772828639152 68-69 0.0438360880993788 70-71 0.05634929870229238 72-73 0.002709867264325235 74-75 0.012274104667826065 76-77 0.01912847480700166 78-79 0.02295416976840199 80-81 0.07635449527128162 82-83 0.028453606275414968 84-85 0.012274104667826065 86-87 0.0299679438643026 88-89 0.04598804151516649 90-91 0.04168413468359112 92-93 0.04184353864031613 94-95 0.012991422473088626 96-97 0.015541885780688847 98-99 0.037300525873653234 100-101 0.07555747548765655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 627337.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.06831099123123 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56436973184431 11.499185487427294 2 9.54340077249549 3.066926630528277 3 4.088476310837094 1.970847265284365 4 2.294358549286616 1.4746586678130986 5 1.609536769886102 1.2931268685175827 6 1.1086804228178602 1.0688773094236264 7 0.8737481208731338 0.9827759573935384 8 0.781774633436225 1.0049438348087247 9 0.5888302545537372 0.8515356886093768 >10 5.550399954616306 19.130558050830555 >50 1.0916852936673045 12.431842761157865 >100 0.8167645466357031 25.856581596881462 >500 0.05698357302109557 6.198171013932837 >1k 0.02799193060685396 9.733465757346957 >5k 0.0029991354221629244 3.436503110044452 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8863 1.4127972684537975 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6533 1.0413860492845155 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5997 0.955945528479908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4979 0.7936723005338437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4584 0.7307077376274634 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4407 0.7024932372871359 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3456 0.5509000744416478 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2979 0.47486438708381623 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2939 0.46848822881481567 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2840 0.4527072370990393 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2827 0.4506349856616141 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2464 0.39277134937043406 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2406 0.3835259198803833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2379 0.37922201304880787 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2363 0.37667154974120765 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2233 0.3559490353669559 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1761 0.2807103677927494 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1692 0.2697114947787234 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1655 0.2638135483798979 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1508 0.24038116674132085 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1483 0.2363960678231955 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1455 0.2319327570348951 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1308 0.20850037539631808 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1223 0.1949510390746919 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1172 0.1868214372817162 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1149 0.18315514627704088 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1119 0.17837302757529047 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1100 0.1753443523975152 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 0.17359090887354006 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.16211382398933905 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1006 0.1603603804653639 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 994 0.15844753298466374 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 991 0.1579693211144887 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 942 0.150158527234963 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 932 0.14856448766771288 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 888 0.1415507135718123 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 874 0.1393190581776621 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 872 0.13900025026421206 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 869 0.138522038394037 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 823 0.13118945638468638 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 808 0.12879839703381116 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 796 0.126885549553111 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 784 0.12497270207241083 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 783 0.12481329811568584 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 775 0.12353806646188573 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 771 0.12290045063498567 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 761 0.12130641106773553 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 754 0.12019058337066042 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 727 0.11588667653908505 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 726 0.11572727258236004 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 725 0.11556786862563502 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 716 0.1141332330151099 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 691 0.11014813409698455 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 691 0.11014813409698455 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 689 0.10982932618353453 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 686 0.10935111431335949 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 683 0.10887290244318444 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 669 0.10664124704903426 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 654 0.10425018769815904 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 647 0.10313436000108395 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 639 0.10185912834728385 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.970197836250691E-5 0.0 12-13 0.0 0.0 0.0 1.5940395672501382E-4 0.0 14-15 0.0 0.0 0.0 2.3910593508752074E-4 0.0 16-17 0.0 0.0 0.0 4.782118701750415E-4 0.0 18-19 0.0 0.0 0.0 4.782118701750415E-4 0.0 20-21 0.0 0.0 0.0 0.0010361257187125898 0.0 22-23 0.0 0.0 0.0 0.0017534435239751523 0.0 24-25 0.0 0.0 0.0 0.002709867264325235 0.0 26-27 0.0 0.0 0.0 0.003905396939762839 0.0 28-29 0.0 0.0 0.0 0.007970197836250692 0.0 30-31 0.0 0.0 0.0 0.01442605808361375 0.0 32-33 0.0 0.0 0.0 0.023193275703489512 0.0 34-35 0.0 0.0 0.0 0.03387334080406544 0.0 36-37 0.0 0.0 0.0 0.04901671669294175 0.0 38-39 0.0 0.0 0.0 0.06981893304555606 0.0 40-41 0.0 0.0 0.0 0.09253399687887053 0.0 42-43 0.0 0.0 0.0 0.11397382905838488 0.0 44-45 0.0 0.0 0.0 0.132384986060124 0.0 46-47 0.0 0.0 0.0 0.15836783100630125 0.0 48-49 0.0 0.0 0.0 0.17590226624605276 0.0 50-51 0.0 0.0 0.0 0.19805941623082968 0.0 52-53 0.0 0.0 0.0 0.23934504102260826 0.0 54-55 0.0 0.0 0.0 0.3102798017652394 0.0 56-57 0.0 0.0 0.0 0.4313471068978874 0.0 58-59 0.0 0.0 0.0 0.5887585141638385 0.0 60-61 0.0 0.0 0.0 0.7696820050467292 0.0 62-63 0.0 0.0 0.0 0.8749683184636009 0.0 64-65 0.0 0.0 0.0 0.9990642987740241 0.0 66-67 0.0 0.0 0.0 1.227171360847519 0.0 68-69 0.0 0.0 0.0 1.5411174536174337 0.0 70-71 0.0 0.0 0.0 1.8530709969282857 0.0 72-73 0.0 0.0 0.0 2.1655027521093126 0.0 74-75 0.0 0.0 0.0 2.4812819903815653 0.0 76-77 0.0 0.0 0.0 2.752587524727539 0.0 78-79 0.0 0.0 0.0 3.0164010731074367 0.0 80-81 0.0 0.0 0.0 3.2554273062165953 0.0 82-83 0.0 0.0 0.0 3.553034493422196 0.0 84-85 0.0 0.0 0.0 4.06025788372119 0.0 86-87 0.0 0.0 0.0 4.711502748921234 0.0 88-89 0.0 0.0 0.0 5.464845848403649 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGATGC 85 7.183189E-9 44.726604 7 GGCATTC 195 0.0 38.992424 7 CCTCATC 100 3.0104275E-8 38.02368 3 CTTTAGG 205 0.0 37.09627 2 CATTCCG 205 0.0 37.090355 9 TAGGCAT 215 0.0 35.373688 5 CCTTTAG 220 0.0 34.564224 1 TGGGAGT 55 0.0025718743 34.564224 6 CTCCCGC 155 3.6379788E-11 33.725304 7 GTATGGT 85 1.6249713E-5 33.54763 1 CATATAG 295 0.0 32.223457 3 TTAGGCA 240 0.0 31.688929 4 GTAAACT 75 3.0642073E-4 31.688927 4 GGTACTC 165 7.6397555E-11 31.686398 3 ATACTCG 75 3.067077E-4 31.68387 6 CGCAGAA 135 1.1899829E-8 31.681343 8 GTCCGTT 30 0.0039490676 31.669977 20-21 GACATAT 305 0.0 31.164463 1 GTGGTAC 175 1.5097612E-10 29.873365 1 GGCTTAT 80 4.477072E-4 29.70363 1 >>END_MODULE