##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937140_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 627337 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.908594583134743 31.0 31.0 34.0 30.0 34.0 2 32.03776598542729 33.0 31.0 34.0 30.0 34.0 3 32.1354136612379 33.0 31.0 34.0 30.0 34.0 4 35.85931644395277 37.0 35.0 37.0 35.0 37.0 5 35.69138118746383 37.0 35.0 37.0 33.0 37.0 6 35.80561962709039 37.0 35.0 37.0 35.0 37.0 7 35.756507268023405 37.0 35.0 37.0 33.0 37.0 8 35.72481935546604 37.0 35.0 37.0 33.0 37.0 9 37.342547944725084 39.0 37.0 39.0 34.0 39.0 10-11 37.26365892654188 39.0 37.0 39.0 33.5 39.0 12-13 37.19966780215418 39.0 37.0 39.0 33.0 39.0 14-15 38.23611232878022 40.0 37.5 41.0 33.0 41.0 16-17 38.23736285282074 40.0 37.5 41.0 33.5 41.0 18-19 38.432975418315834 40.0 38.0 41.0 34.0 41.0 20-21 38.4789977316817 40.0 38.0 41.0 34.0 41.0 22-23 38.35639935154471 40.0 38.0 41.0 34.0 41.0 24-25 38.23015938801633 40.0 37.5 41.0 33.5 41.0 26-27 37.97967998061648 40.0 37.0 41.0 33.0 41.0 28-29 37.85438050043278 40.0 37.0 41.0 33.0 41.0 30-31 37.72510468854858 40.0 37.0 41.0 33.0 41.0 32-33 37.47231711185535 39.5 36.0 41.0 33.0 41.0 34-35 37.27697393904711 39.0 35.0 41.0 33.0 41.0 36-37 37.03520436384272 39.0 35.0 41.0 31.5 41.0 38-39 36.82865190479758 39.0 35.0 41.0 31.0 41.0 40-41 36.593203493497114 39.0 35.0 41.0 30.5 41.0 42-43 36.299539960180894 39.0 35.0 41.0 30.0 41.0 44-45 35.935046075713686 39.0 35.0 40.0 30.0 41.0 46-47 35.41312723464422 39.0 35.0 40.0 27.5 41.0 48-49 34.88682319072524 38.5 35.0 40.0 24.0 41.0 50-51 34.616189544056866 38.0 34.5 40.0 23.5 41.0 52-53 34.463024658198066 38.0 34.0 40.0 23.0 41.0 54-55 34.12149052263776 38.0 34.0 40.0 21.5 41.0 56-57 33.92921109387777 38.0 33.5 40.0 21.0 41.0 58-59 33.668520269010116 38.0 33.0 40.0 19.5 41.0 60-61 33.26160102146055 38.0 33.0 40.0 18.0 41.0 62-63 32.971832523826905 37.0 32.5 39.5 17.0 40.5 64-65 32.70269807137153 37.0 32.0 39.0 13.5 40.0 66-67 32.33135858398278 36.0 31.5 39.0 10.5 40.0 68-69 31.81633476106144 36.0 31.0 38.5 8.0 40.0 70-71 31.356567522719047 35.0 31.0 38.0 7.0 40.0 72-73 30.899982784372675 35.0 30.5 37.0 2.0 39.0 74-75 30.39924315001347 34.5 30.0 37.0 2.0 39.0 76-77 28.920003921337337 33.0 28.5 35.0 2.0 37.5 78-79 29.57948678302093 34.0 29.0 36.0 2.0 38.0 80-81 29.343887734981358 34.0 29.0 35.5 2.0 37.0 82-83 28.94464538198767 34.0 29.0 35.0 2.0 37.0 84-85 28.463363391606105 34.0 29.0 35.0 2.0 36.0 86-87 28.181962007660957 34.0 29.0 35.0 2.0 36.0 88-89 27.749286268783763 33.5 27.5 35.0 2.0 35.5 90-91 27.241689873226033 33.0 26.0 35.0 2.0 35.0 92-93 26.772943410001325 33.0 25.5 35.0 2.0 35.0 94-95 26.187083656790527 32.0 25.0 34.0 2.0 35.0 96-97 25.38928598185664 32.0 21.5 34.0 2.0 35.0 98-99 24.63083318854141 32.0 10.5 34.0 2.0 35.0 100-101 21.597066648388346 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26736137939337823 1101 2 0.4044108877125794 1101 3 0.21058084937489951 1101 4 0.28215029178812756 1101 5 0.17867730675055782 1101 6 0.2615609302031743 1101 7 0.3008082925797524 1101 8 0.42661428842134796 1101 9 0.5520587113173789 1101 10-11 0.4174251931601347 1101 12-13 0.3672317467471444 1101 14-15 0.5308480518930949 1101 16-17 0.3425783096654911 1101 18-19 0.2640745421808788 1101 20-21 0.34471655110211685 1101 22-23 0.3030636943115965 1101 24-25 0.44815351385120294 1101 26-27 0.4967651240153188 1101 28-29 0.47777597925513504 1101 30-31 0.5201432273167725 1101 32-33 0.6518341937677405 1101 34-35 0.45433764895997086 1101 36-37 0.49939059095304117 1101 38-39 0.6372211389210705 1101 40-41 0.79025982104951 1101 42-43 0.8470520958061769 1101 44-45 0.5175634349186851 1101 46-47 0.5067142811529166 1101 48-49 0.5305908647692448 1101 50-51 0.6029231268187587 1101 52-53 0.6776168456218841 1101 54-55 0.2970221725551667 1101 56-57 0.48692857992746497 1101 58-59 0.6120375691746602 1101 60-61 0.5466782453405514 1101 62-63 0.7003329687331785 1101 64-65 0.8488162060209063 1101 66-67 0.5050006919380721 1101 68-69 0.29341299608035953 1101 70-71 0.6167152093549966 1101 72-73 0.7416310018599326 1101 74-75 0.6473571594180392 1101 76-77 0.5938605592231632 1101 78-79 0.7329585011232815 1101 80-81 0.8577845199958709 1101 82-83 1.0973108143683845 1101 84-85 0.6803373430846804 1101 86-87 0.4585457898602563 1101 88-89 0.699073682073795 1101 90-91 0.927434248033677 1101 92-93 0.9479382489459063 1101 94-95 0.9636973798156383 1101 96-97 0.9392672452507469 1101 98-99 1.3648802109604077 1101 100-101 1.6030931981775431 1102 1 -0.16571443539199748 1102 2 0.015509854326253958 1102 3 0.06369839706965763 1102 4 0.1449061542682557 1102 5 0.03374239021637493 1102 6 0.1210228737643817 1102 7 0.16523098733333086 1102 8 0.21889370018605092 1102 9 0.30972961751769645 1102 10-11 0.33359545349399156 1102 12-13 0.3826977642352176 1102 14-15 0.3492850550727482 1102 16-17 0.15328851435547364 1102 18-19 0.11174321268961762 1102 20-21 0.052054587668095564 1102 22-23 0.010186856076309425 1102 24-25 -0.02092811373226766 1102 26-27 0.09453067449226893 1102 28-29 -0.007100809298130173 1102 30-31 0.10497033383504828 1102 32-33 0.11667173351337112 1102 34-35 0.4306529808359656 1102 36-37 0.2024497622566912 1102 38-39 0.4742541277922854 1102 40-41 0.32617869028639745 1102 42-43 0.5842726343594364 1102 44-45 0.3307465628996056 1102 46-47 0.2987191996743874 1102 48-49 0.6254338695387176 1102 50-51 0.6069237726852137 1102 52-53 0.756983896496287 1102 54-55 0.8781194002976136 1102 56-57 0.8345388740451227 1102 58-59 0.41549419675813226 1102 60-61 0.45979927077774363 1102 62-63 0.6735319949652947 1102 64-65 0.7319864166727399 1102 66-67 0.8274557296964176 1102 68-69 0.5561663744587371 1102 70-71 0.4599162228685074 1102 72-73 0.681028855596022 1102 74-75 0.6154786816755902 1102 76-77 0.6124602710673592 1102 78-79 0.2344129426972117 1102 80-81 0.7111107322375254 1102 82-83 0.5978461382952531 1102 84-85 0.429615711526651 1102 86-87 0.48700365949459723 1102 88-89 0.29355897619144145 1102 90-91 0.6468226621831228 1102 92-93 0.5159330820142713 1102 94-95 1.0632949553323279 1102 96-97 1.2317615318167228 1102 98-99 0.5757469140605664 1102 100-101 0.6699627331536924 1103 1 0.10238232953908977 1103 2 0.1093364240046597 1103 3 0.13135010074500286 1103 4 0.10143611215492854 1103 5 0.07715979067579326 1103 6 0.016445442677088806 1103 7 -0.0784855134323692 1103 8 0.28077157154898913 1103 9 0.20134523773209168 1103 10-11 0.057251894918188384 1103 12-13 0.12159037526124905 1103 14-15 0.16180898309725222 1103 16-17 0.004141548428975739 1103 18-19 -0.07503296456918918 1103 20-21 0.08162484034803441 1103 22-23 0.1533924935188793 1103 24-25 0.051022259544915016 1103 26-27 0.2022055883513758 1103 28-29 0.1135538614522531 1103 30-31 0.3564155865149914 1103 32-33 0.19504797777064198 1103 34-35 0.17669648198451426 1103 36-37 0.12468487902545888 1103 38-39 0.2006802380526338 1103 40-41 0.24685369985760275 1103 42-43 0.15231556123539747 1103 44-45 0.06190324360404986 1103 46-47 -0.08757841709437741 1103 48-49 -7.625316863979492E-4 1103 50-51 -0.349332209320913 1103 52-53 -0.39546154760784447 1103 54-55 -0.11357877160131125 1103 56-57 -0.2219264781754049 1103 58-59 -0.3032354644210429 1103 60-61 -0.5511166940001928 1103 62-63 -0.3495992263210752 1103 64-65 -0.45989959021854077 1103 66-67 -0.12350976609224773 1103 68-69 -0.276141244224263 1103 70-71 -0.5910576477287321 1103 72-73 -0.25270965895221664 1103 74-75 -0.6847677769767841 1103 76-77 -0.5090641833736846 1103 78-79 -0.5521813815753767 1103 80-81 -0.6545932302433322 1103 82-83 -0.32587969439999753 1103 84-85 -0.7003649067735012 1103 86-87 -0.5831341659265377 1103 88-89 -0.31452869609033485 1103 90-91 -0.49289068008334525 1103 92-93 -0.30231921507608206 1103 94-95 -0.12027606304439331 1103 96-97 0.08965179789635869 1103 98-99 0.014178910614930373 1103 100-101 0.17076910757942088 1104 1 -0.14787782559971419 1104 2 0.08388893444494983 1104 3 -0.05556625426565631 1104 4 0.0469693961080182 1104 5 0.07048664095821522 1104 6 0.021407703141235856 1104 7 0.024624533327390452 1104 8 0.11957990196646762 1104 9 0.016035255499893708 1104 10-11 0.10540786076402497 1104 12-13 0.12389769005123696 1104 14-15 0.08560738444960236 1104 16-17 0.04408274655131805 1104 18-19 0.05236125794183977 1104 20-21 0.04937469152566365 1104 22-23 0.0783735215065704 1104 24-25 -0.04079319162543982 1104 26-27 -0.07328507105669502 1104 28-29 0.20903420553866425 1104 30-31 0.19656899852348175 1104 32-33 -0.008629638890191416 1104 34-35 0.03464268780926005 1104 36-37 0.01464607160387743 1104 38-39 -0.009184299507410287 1104 40-41 0.21094547441101952 1104 42-43 -0.1527521245426442 1104 44-45 -0.4627206106908943 1104 46-47 -0.5739787788122612 1104 48-49 -0.36353126399243507 1104 50-51 -0.22939160194386687 1104 52-53 -0.10424570291024082 1104 54-55 -0.08851867462820451 1104 56-57 -0.0984266304059247 1104 58-59 -0.2194905027967664 1104 60-61 -0.06791771735282026 1104 62-63 -0.15984615785369272 1104 64-65 -0.31084092012251574 1104 66-67 -0.18393846540744363 1104 68-69 -0.32227390744037265 1104 70-71 -0.04929649968342176 1104 72-73 -0.1118680286769731 1104 74-75 -0.32986163731550633 1104 76-77 -0.013548167359939356 1104 78-79 0.015516892993947096 1104 80-81 0.04227263876571641 1104 82-83 0.16015372360682534 1104 84-85 0.22791179832190167 1104 86-87 -0.18124559866564027 1104 88-89 -0.00942692885306684 1104 90-91 0.1774189158923214 1104 92-93 0.08123352711812615 1104 94-95 0.2037715873798085 1104 96-97 0.517812626030377 1104 98-99 0.4870761981851963 1104 100-101 1.1505497138658605 1105 1 -0.05768391213618429 1105 2 0.10315520316346749 1105 3 0.10813880404639775 1105 4 -0.05047902015034822 1105 5 -0.013677667923161607 1105 6 0.09867549004345477 1105 7 0.03027459198449378 1105 8 0.09462044437209727 1105 9 0.025717989610726022 1105 10-11 0.019841819773070313 1105 12-13 -0.07449700320663766 1105 14-15 0.20357284577041668 1105 16-17 -0.04789244494685363 1105 18-19 -2.6623498480660146E-4 1105 20-21 -0.13697157512260816 1105 22-23 0.16874426886700178 1105 24-25 -0.01095354977877605 1105 26-27 -0.0506546452751806 1105 28-29 -0.10579266976323964 1105 30-31 -0.06494245686262445 1105 32-33 0.1132196986296492 1105 34-35 0.1480039982778223 1105 36-37 0.05693740760553112 1105 38-39 0.1467831975597349 1105 40-41 0.1845907542398777 1105 42-43 0.07451609366177081 1105 44-45 0.2716985977833275 1105 46-47 0.10153533339531151 1105 48-49 -0.4014556653450043 1105 50-51 -0.41682259359385654 1105 52-53 -0.15907715001534228 1105 54-55 0.048704429367383284 1105 56-57 -0.07018496316418066 1105 58-59 -0.2508030416139633 1105 60-61 -0.18126177573387992 1105 62-63 0.05808868101180309 1105 64-65 -0.18963422286215348 1105 66-67 -0.2849170900710263 1105 68-69 -0.43285978833196026 1105 70-71 -0.6699166259687033 1105 72-73 -0.666645563008629 1105 74-75 -0.538918120649992 1105 76-77 -0.4265113859093823 1105 78-79 -0.07149185381441825 1105 80-81 -0.025989558460150164 1105 82-83 -0.03254993728614508 1105 84-85 -0.33806234079893116 1105 86-87 -0.14933354980772862 1105 88-89 -0.03609218659925517 1105 90-91 0.1148822552063784 1105 92-93 0.40386912852589774 1105 94-95 -0.04927771908627321 1105 96-97 0.02661981670044611 1105 98-99 0.107523803595452 1105 100-101 0.5314089540839255 1106 1 0.21330238031562487 1106 2 0.13188815914740815 1106 3 0.12489347871817813 1106 4 0.16343016282191059 1106 5 0.0909328062350383 1106 6 0.11661239553732372 1106 7 0.16553716929288953 1106 8 0.2796926892995799 1106 9 0.4356627434897078 1106 10-11 0.24520485279415283 1106 12-13 0.40393124012945947 1106 14-15 0.3168929477632574 1106 16-17 0.2501088390055486 1106 18-19 0.1922678530473121 1106 20-21 0.12256877895891449 1106 22-23 0.22068330825360505 1106 24-25 0.4375538555523377 1106 26-27 0.40125817254824625 1106 28-29 0.3551270289600339 1106 30-31 0.23172966509476822 1106 32-33 0.16052136415100904 1106 34-35 0.09993150921854976 1106 36-37 0.15306091116805476 1106 38-39 0.3763439411671001 1106 40-41 0.5861502591044356 1106 42-43 0.586341792602056 1106 44-45 0.40023285761188987 1106 46-47 0.3039522381658273 1106 48-49 -0.06678036063235737 1106 50-51 -0.1515168863349956 1106 52-53 -0.20445175669188842 1106 54-55 0.058315988555776244 1106 56-57 0.2882276376532076 1106 58-59 0.08453458361660182 1106 60-61 0.09942310437028112 1106 62-63 -0.1590812437594309 1106 64-65 -0.11983852500689096 1106 66-67 -0.1608133702258172 1106 68-69 -0.26133459754748145 1106 70-71 -0.2539081356073858 1106 72-73 0.228977922470051 1106 74-75 -0.01906884942860998 1106 76-77 -0.14116652714414712 1106 78-79 -0.018062028142601605 1106 80-81 0.1744539476341025 1106 82-83 0.4826913463045841 1106 84-85 0.4723681901425145 1106 86-87 0.6093986828227429 1106 88-89 0.5423634085398064 1106 90-91 0.7850929771131341 1106 92-93 0.5705501115632572 1106 94-95 0.9431112461565903 1106 96-97 0.6860152740406775 1106 98-99 0.6947071104058224 1106 100-101 0.9509867417073501 1107 1 0.05181815663254241 1107 2 0.1923886886820867 1107 3 0.18853543694695674 1107 4 -0.025885640210276506 1107 5 0.006278816596740455 1107 6 0.006998717322666437 1107 7 0.1311627357995846 1107 8 0.08910075540077145 1107 9 0.022766427333650086 1107 10-11 0.10914664214430303 1107 12-13 0.151275218222942 1107 14-15 0.16322293850833347 1107 16-17 0.08737114472356922 1107 18-19 0.11114409459145236 1107 20-21 0.13773326864968993 1107 22-23 0.2331881981008479 1107 24-25 0.22940259945792008 1107 26-27 0.26232687847998903 1107 28-29 0.40493906800248425 1107 30-31 0.24457922954056954 1107 32-33 0.0434834666422006 1107 34-35 0.21575171549854844 1107 36-37 0.4263330061217232 1107 38-39 0.3817311216573245 1107 40-41 0.3938886212679904 1107 42-43 0.49657612362324244 1107 44-45 0.8050084494026777 1107 46-47 0.23960781163757616 1107 48-49 0.2314538081890234 1107 50-51 0.1817560196177297 1107 52-53 0.521842025330642 1107 54-55 0.6702445920154076 1107 56-57 0.3773111133089202 1107 58-59 0.4335492197642594 1107 60-61 0.44233377998020273 1107 62-63 0.46037056858492065 1107 64-65 0.5457996835439047 1107 66-67 0.7533328385921223 1107 68-69 0.4447975094280672 1107 70-71 0.4469465142795528 1107 72-73 0.5135825365776192 1107 74-75 0.3477543718596401 1107 76-77 0.40643553947226607 1107 78-79 0.5651405117156152 1107 80-81 0.6731026800902846 1107 82-83 0.6044747809332911 1107 84-85 0.5192773296248099 1107 86-87 0.6607766656295659 1107 88-89 0.6216648044122977 1107 90-91 0.47754543825674034 1107 92-93 0.7037781740388098 1107 94-95 0.6436438817126984 1107 96-97 0.5566704658658033 1107 98-99 0.8801420444286663 1107 100-101 1.396640263828541 1108 1 -0.014183230098723243 1108 2 0.14380921054943485 1108 3 0.06682563240585182 1108 4 7.694411065912732E-4 1108 5 -0.03319475212983747 1108 6 0.1757103737643817 1108 7 0.1407272319685191 1108 8 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-0.791982003572997 2316 60-61 -0.7915873186262203 2316 62-63 -0.5517266970876875 2316 64-65 -0.6394022422676642 2316 66-67 -0.7296893448068928 2316 68-69 -0.6974139235545032 2316 70-71 -0.429373926138112 2316 72-73 -0.7154529324834478 2316 74-75 -0.6541094805760643 2316 76-77 -0.5688569888664148 2316 78-79 -1.0005260059782835 2316 80-81 -1.2444459730604898 2316 82-83 -1.105739333559054 2316 84-85 -1.1190235682746739 2316 86-87 -1.0757446848762662 2316 88-89 -1.7242804277820696 2316 90-91 -1.6559815596711864 2316 92-93 -1.0948698981181835 2316 94-95 -1.6704177930120423 2316 96-97 -1.5373144201700306 2316 98-99 -1.4224267200453937 2316 100-101 -2.7189135085681606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 28.0 6 88.0 7 144.0 8 301.0 9 559.0 10 983.0 11 1665.0 12 2416.0 13 3280.0 14 4077.0 15 5797.0 16 7744.0 17 10774.0 18 8951.0 19 4724.0 20 3718.0 21 3831.0 22 4166.0 23 4479.0 24 5492.0 25 6326.0 26 7545.0 27 9391.0 28 11586.0 29 14109.0 30 17267.0 31 20906.0 32 25481.0 33 32668.0 34 43548.0 35 59227.0 36 85492.0 37 132965.0 38 84177.0 39 3430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.59824425180605 24.782325170560444 17.07485845755002 18.544572120083483 2 13.948064783523561 25.66266020531786 39.286807371038705 21.102467640119876 3 18.05335267440841 29.288498170833765 34.92025791642424 17.737891238333585 4 10.401745792134053 21.435528272682784 43.539756143827 24.622969791356162 5 9.049115205771846 41.86612595465479 37.445103862296975 11.639654977276386 6 22.560601399247933 44.04857357369325 18.892397547091914 14.498427479966908 7 21.38793467605028 38.29229996732209 24.980592506396103 15.339172850231536 8 22.938069968772766 34.25463506855167 22.27367427714291 20.533620685532654 9 23.47892759394074 19.428791861471588 26.356966032610863 30.735314511976817 10-11 21.21244243524613 29.10477143863665 30.507685661773497 19.175100464343725 12-13 23.881426195051695 29.155650635934165 29.73367840709192 17.22924476192222 14-15 20.887937397081867 29.834262950747547 31.11710310065533 18.160696551515258 16-17 16.002324109689052 32.88176849125749 31.9638408064565 19.15206659259696 18-19 15.896996104955004 32.17903338803559 35.215446917956974 16.70852358905243 20-21 17.603531785029126 29.410606672867534 34.920771553795724 18.065089988307616 22-23 17.37617113867956 29.464195336566622 35.11921603946902 18.0404174852848 24-25 18.49042497993498 30.426860906165835 34.67785578848957 16.40485832540961 26-27 17.512784217707893 28.966534679980104 37.37144050397235 16.149240598339645 28-29 15.951101164288357 31.052338778581174 36.20651453128786 16.79004552584261 30-31 17.15003259810915 29.2245635121155 35.71461590819607 17.91078798157928 32-33 17.489164516041615 30.065180277904858 35.90135764349942 16.544297562554096 34-35 18.450768884985262 29.12445782729219 35.520063379013195 16.904709908709354 36-37 18.274339457219142 28.412067110349497 36.16857290877934 17.145020523652015 38-39 16.690539559256187 30.952739643588473 35.21933170248398 17.137389094671363 40-41 16.577479584208067 30.60153060655186 35.698185812000965 17.122803997239107 42-43 16.609653184811354 30.658242698900274 34.02549188075946 18.70661223552891 44-45 17.80103501896525 29.18241790823512 34.28570062239821 18.730846450401422 46-47 19.16899396814466 30.861292831911445 33.48970886414936 16.480004335794536 48-49 17.999974495366924 30.836775130432287 33.267207258618576 17.896043115582213 50-51 16.135575658550195 31.104770254652692 34.601602040409674 18.15805204638744 52-53 17.58971076879915 32.659398360207575 32.03971399673699 17.711176874256278 54-55 17.464935114619415 30.681834484495575 32.46596327014029 19.38726713074472 56-57 18.74424650885131 31.038366171902958 31.81522197417176 18.402165345073975 58-59 19.550496782431136 29.975754658182126 32.28583679904102 18.187911760345717 60-61 18.51360054070598 31.5755075494797 33.03096625688635 16.879925652927973 62-63 17.42468561554635 31.277845878690403 33.33973605892845 17.957732446834797 64-65 17.522719048932235 32.34935927579594 32.393354767852045 17.734566907419776 66-67 18.212778777594817 31.12641211980164 30.823863409937562 19.83694569266598 68-69 17.45652844450856 30.55318884392801 32.4840535885503 19.506229123013124 70-71 19.058378883304403 31.091780601371198 32.30054851251249 17.549292002811903 72-73 19.144586456337453 29.061393133482323 33.85708410511785 17.936936305062375 74-75 17.317453030201587 32.95282456425609 31.512885012067244 18.21683739347508 76-77 17.692896874325022 31.024498016614686 32.03767037307729 19.244934735983 78-79 17.84199657439532 31.272152256890063 32.06359605601158 18.822255112703033 80-81 18.75334256299162 30.82100121866559 31.391916955392375 19.033739262950412 82-83 17.790437994251892 31.056752590712804 32.77409111848974 18.378718296545557 84-85 17.20727154475692 31.24456020990815 31.752672471234867 19.795495774100065 86-87 17.077983976637647 31.625258634156605 31.695874924681274 19.60088246452448 88-89 17.77931517101596 30.874212628392485 31.07267450896625 20.273797691625305 90-91 17.847732210690083 29.29746592298459 32.51930008018121 20.33550178614411 92-93 18.793248286008957 30.833108839427613 32.236979486304804 18.136663388258622 94-95 18.181455900098353 29.662525883217473 32.54845481774549 19.607563398938687 96-97 17.367688210994295 29.825635287075713 32.83662862476079 19.970047877169197 98-99 17.59459549933129 30.89033027326969 30.8935183930601 20.62155583433892 100-101 16.555294305184713 30.067747977523613 32.19782409436895 21.179133622922727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4414.0 1 2783.0 2 1076.5 3 918.5 4 727.5 5 726.5 6 1042.5 7 1858.5 8 2729.5 9 2978.5 10 2464.5 11 1837.0 12 1647.5 13 1525.5 14 1438.5 15 1383.5 16 1369.0 17 1442.0 18 1526.0 19 1681.5 20 1971.5 21 2310.5 22 2663.0 23 2981.0 24 3361.0 25 4480.0 26 7370.5 27 10383.5 28 10657.0 29 11147.0 30 13850.5 31 16310.0 32 19686.5 33 25000.5 34 31491.0 35 34664.0 36 37202.5 37 41663.5 38 38942.0 39 32199.0 40 27504.5 41 23627.5 42 20216.5 43 18469.0 44 17845.5 45 16609.5 46 14790.0 47 18986.0 48 32152.0 49 29705.0 50 12982.0 51 4370.5 52 1784.0 53 1062.0 54 725.0 55 558.0 56 463.0 57 424.5 58 427.0 59 470.5 60 491.0 61 438.0 62 334.5 63 246.0 64 187.0 65 158.0 66 127.5 67 116.5 68 96.0 69 58.0 70 43.0 71 37.5 72 31.5 73 24.0 74 20.0 75 18.5 76 13.5 77 7.0 78 6.0 79 6.0 80 2.0 81 0.5 82 2.0 83 1.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022316553941501935 2 0.002709867264325235 3 3.1880791345002764E-4 4 0.0 5 0.002550463307600221 6 0.0 7 3.1880791345002764E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.005021224636837936 14-15 0.002869271221050249 16-17 0.0 18-19 2.3910593508752074E-4 20-21 8.767217619875761E-4 22-23 0.0015143375888876313 24-25 2.3910593508752074E-4 26-27 1.5940395672501382E-4 28-29 1.5940395672501382E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001912847480700166 38-39 0.009006323554963282 40-41 6.376158269000553E-4 42-43 0.0 44-45 8.767217619875761E-4 46-47 1.5940395672501382E-4 48-49 0.0 50-51 0.001912847480700166 52-53 2.3910593508752074E-4 54-55 0.0 56-57 7.970197836250691E-5 58-59 0.0 60-61 0.0014346356105251245 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.3910593508752074E-4 70-71 6.376158269000553E-4 72-73 4.782118701750415E-4 74-75 0.0030286751777752626 76-77 7.970197836250691E-5 78-79 3.9850989181253454E-4 80-81 0.001833145502337659 82-83 0.0 84-85 0.0023910593508752074 86-87 4.782118701750415E-4 88-89 0.0019925494590626726 90-91 0.0012752316538001106 92-93 0.0 94-95 0.0 96-97 3.9850989181253454E-4 98-99 0.0012752316538001106 100-101 0.001912847480700166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 627337.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.966407503725483 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98799295745624 11.33423223430341 2 9.98999900891683 3.1900879027635956 3 4.1640094342225105 1.9945281442846197 4 2.309999843594862 1.4752959534551076 5 1.5709998448040472 1.2541611855215455 6 1.1539998855209634 1.1055139458888155 7 0.8989999108125022 1.0047659245291691 8 0.7349999270824701 0.9388246680805786 9 0.5919999412691455 0.8506901074036255 >10 5.613999443048956 19.072350863304294 >50 1.0759998932527035 12.16209038124181 >100 0.8149999191458673 25.33102231978228 >500 0.05999999404754852 6.4528229524619976 >1k 0.027999997222189307 9.393994586936882 >5k 0.003999999603169902 4.43961883004227 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9880 1.5749110924431364 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6733 1.073266840629518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5605 0.8934591774437025 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5588 0.8907493101793772 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4803 0.7656172041502415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4264 0.6796984714754589 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3982 0.6347465556790051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3867 0.6164151006556284 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2940 0.46864763277154065 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2755 0.43915790077741307 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2596 0.4138126716581359 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2586 0.4122186320908858 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2570 0.40966816878328555 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2563 0.40855234108621047 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2402 0.3828883040534832 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2138 0.34080565947807956 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1767 0.2816667915330994 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1637 0.2609442771588476 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1612 0.2569591782407223 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1602 0.25536513867347216 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1490 0.23751189552027058 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1440 0.2295416976840199 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1347 0.21471712970859363 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1290 0.20563110417526784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1226 0.19542925094486696 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1190 0.18969070850276645 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1176 0.18745905310861627 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1152 0.18363335814721593 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1150 0.18331455023376592 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1123 0.17901064340219053 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1119 0.17837302757529047 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1049 0.1672147506045395 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 942 0.150158527234963 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 938 0.14952091140806298 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 909 0.14489819666303758 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 902 0.14378236896596247 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 900 0.14346356105251246 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 884 0.14091309774491223 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 865 0.13788442256713695 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 864 0.13772501861041195 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 864 0.13772501861041195 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 859 0.13692799882678688 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 825 0.13150826429813642 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 812 0.1294360128607112 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 805 0.12832018516363614 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 788 0.1256103178993109 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 748 0.11923415963031034 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 738 0.11764012006306021 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 735 0.11716190819288516 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 716 0.1141332330151099 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 712 0.11349561718820986 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 708 0.1128580013613098 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 706 0.11253919344785977 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 704 0.11222038553440974 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 703 0.11206098157768472 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 684 0.10903230639990946 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 682 0.10871349848645945 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 674 0.10743826683265931 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 663 0.10568482330868417 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 662 0.10552541935195915 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 661 0.10536601539523414 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 657 0.10472839956833409 No Hit AACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCA 650 0.10361257187125898 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 642 0.10233734021745887 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 0.10090270460693376 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 633 0.10090270460693376 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 632 0.10074330065020873 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.970197836250691E-5 0.0 12-13 0.0 0.0 0.0 1.5940395672501382E-4 0.0 14-15 0.0 0.0 0.0 2.3910593508752074E-4 0.0 16-17 0.0 0.0 0.0 4.782118701750415E-4 0.0 18-19 0.0 0.0 0.0 4.782118701750415E-4 0.0 20-21 0.0 0.0 0.0 0.0010361257187125898 0.0 22-23 0.0 0.0 0.0 0.0017534435239751523 0.0 24-25 0.0 0.0 0.0 0.0025504633076002215 0.0 26-27 0.0 0.0 0.0 0.003745992983037825 0.0 28-29 0.0 0.0 0.0 0.008209303771338211 0.0 30-31 0.0 0.0 0.0 0.014744865997063778 0.0 32-33 0.0 0.0 0.0 0.02399029548711458 0.0 34-35 0.0 0.0 0.0 0.03594559224149062 0.0 36-37 0.0 0.0 0.0 0.05172658395726699 0.0 38-39 0.0 0.0 0.0 0.07372432998531889 0.0 40-41 0.0 0.0 0.0 0.0967582017320834 0.0 42-43 0.0 0.0 0.0 0.11931386160867286 0.0 44-45 0.0 0.0 0.0 0.138522038394037 0.0 46-47 0.0 0.0 0.0 0.1647439892753018 0.0 48-49 0.0 0.0 0.0 0.18243782847177833 0.0 50-51 0.0 0.0 0.0 0.20443557449983024 0.0 52-53 0.0 0.0 0.0 0.2457211992916088 0.0 54-55 0.0 0.0 0.0 0.31721387388277755 0.0 56-57 0.0 0.0 0.0 0.4391579007774131 0.0 58-59 0.0 0.0 0.0 0.5975257317837144 0.0 60-61 0.0 0.0 0.0 0.7779710107964299 0.0 62-63 0.0 0.0 0.0 0.883018218278214 0.0 64-65 0.0 0.0 0.0 1.0073533045237248 0.0 66-67 0.0 0.0 0.0 1.235380664618857 0.0 68-69 0.0 0.0 0.0 1.5496455653022219 0.0 70-71 0.0 0.0 0.0 1.8614397046563491 0.0 72-73 0.0 0.0 0.0 2.1743496717075512 0.0 74-75 0.0 0.0 0.0 2.4925996713090415 0.0 76-77 0.0 0.0 0.0 2.765100735330452 0.0 78-79 0.0 0.0 0.0 3.030906833169413 0.0 80-81 0.0 0.0 0.0 3.2712082979323713 0.0 82-83 0.0 0.0 0.0 3.5693733989865093 0.0 84-85 0.0 0.0 0.0 4.076835895220591 0.0 86-87 0.0 0.0 0.0 4.729116886139348 0.0 88-89 0.0 0.0 0.0 5.4815035618814125 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGTGC 45 9.619342E-4 42.22435 8 ATGTATT 50 0.0016154896 37.998882 1 GTATGGT 65 1.3277262E-4 36.537388 1 GTGCTGA 55 0.002579108 34.544437 1 GCTTTGC 110 7.005838E-8 34.544437 1 CATGGGT 255 0.0 33.528427 4 ATGCCTC 30 0.003951656 31.665737 66-67 TCGGTTC 30 0.003951656 31.665737 50-51 GACATAT 335 0.0 31.193113 1 CATATAG 370 0.0 29.52616 3 ATGGGTA 195 1.6370905E-11 29.22991 5 CTTTGCG 135 4.2210377E-7 28.147322 2 TGGGAGT 85 6.402024E-4 27.942583 6 GTAGTAT 85 6.405009E-4 27.940357 5 AGGCTTA 330 0.0 27.349863 6 CGCAGAA 105 6.898552E-5 27.144224 9 GCATGCA 35 0.008391429 27.14314 30-31 TATCGAG 35 0.008391429 27.14314 68-69 TCTAGAC 70 0.008389917 27.14206 3 ACCTCGT 35 0.00839306 27.14206 64-65 >>END_MODULE