FastQCFastQC Report
Thu 26 May 2016
SRR937139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018020
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133550.4425086646211755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89290.2958562236168084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60230.19956792864195733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80250.043.0503731
GTATCAA138600.040.4238931
TATCAAC177000.031.3049412
ATCAACG177950.031.1640013
TCAACGC178550.031.0597924
CAACGCA180950.030.6730825
AACGCAG185750.029.854876
GTACATG155500.026.0984691
ACGCAGA212050.025.8607237
CGCAGAG213950.025.4294838
TACATGG157950.025.0919252
GACGCGT1201.7076911E-423.7582448
ACATGGG160650.023.723243
GCAGAGT224000.023.4828689
GAGTACT105800.022.31811112-13
AGAGTAC189200.020.95607410-11
CATGGGG79000.020.5724774
ATTCGCG4200.020.35576880-81
AGTACTT111750.020.34328712-13
GTACTTT118300.020.16070414-15
Produced by FastQC (version 0.11.5)