##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937139_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3018020 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.475303344576908 31.0 31.0 34.0 30.0 34.0 2 31.56123120456458 33.0 31.0 34.0 30.0 34.0 3 31.563752725296716 33.0 31.0 34.0 28.0 34.0 4 35.15910099999337 37.0 35.0 37.0 32.0 37.0 5 35.1824229130357 37.0 35.0 37.0 32.0 37.0 6 35.229876872916684 37.0 35.0 37.0 32.0 37.0 7 35.1513899841618 37.0 35.0 37.0 32.0 37.0 8 35.123005480414314 37.0 35.0 37.0 32.0 37.0 9 36.690855594064985 39.0 37.0 39.0 32.0 39.0 10-11 36.542311184153846 39.0 37.0 39.0 32.0 39.0 12-13 36.41577789411601 39.0 37.0 39.0 32.0 39.0 14-15 37.495828722142335 40.0 37.0 41.0 32.0 41.0 16-17 37.423885030582966 40.0 37.0 41.0 32.0 41.0 18-19 37.37390954996985 40.0 37.0 41.0 32.0 41.0 20-21 37.31745564973062 40.0 37.0 41.0 31.0 41.0 22-23 37.168692553395935 39.5 36.5 41.0 31.0 41.0 24-25 36.982356313079435 39.0 36.0 41.0 31.0 41.0 26-27 36.939779226115135 39.0 36.0 41.0 30.5 41.0 28-29 37.010217791797274 39.0 36.0 41.0 31.0 41.0 30-31 36.87596321429281 39.0 36.0 41.0 30.0 41.0 32-33 36.68717520095957 39.0 36.0 41.0 30.0 41.0 34-35 36.48391345981803 39.0 35.0 41.0 30.0 41.0 36-37 36.32387326790412 39.0 35.0 41.0 30.0 41.0 38-39 36.16161075804666 39.0 35.0 41.0 29.5 41.0 40-41 35.94242864527074 39.0 35.0 40.0 28.5 41.0 42-43 35.721555026142966 38.5 35.0 40.0 27.5 41.0 44-45 35.46074860338898 38.0 35.0 40.0 27.0 41.0 46-47 35.10294762791499 38.0 34.0 40.0 25.5 41.0 48-49 34.750110502912506 38.0 34.0 40.0 24.0 41.0 50-51 34.09052590771433 37.5 33.5 39.5 23.5 40.5 52-53 34.39617033684336 38.0 33.5 40.0 24.0 40.5 54-55 34.695122630068724 38.0 34.0 40.0 24.0 41.0 56-57 34.56381352674933 38.0 34.0 40.0 23.5 41.0 58-59 34.3025544893672 38.0 34.0 40.0 23.0 41.0 60-61 34.00624631380839 38.0 33.5 40.0 22.5 41.0 62-63 33.68642719398811 37.0 33.0 40.0 22.0 41.0 64-65 33.362679670777524 36.5 33.0 39.5 21.5 41.0 66-67 32.97983810577796 36.0 32.5 39.0 20.5 41.0 68-69 32.53259719948841 35.5 32.0 39.0 19.0 40.5 70-71 32.178392455981076 35.0 31.5 38.5 18.0 40.0 72-73 31.803413993280365 35.0 31.0 37.5 17.5 40.0 74-75 31.374973326883186 35.0 31.0 37.0 14.0 39.0 76-77 30.941444059350168 35.0 31.0 36.5 10.0 39.0 78-79 30.520285319514116 35.0 30.0 36.0 7.5 38.5 80-81 30.107502435371536 34.0 30.0 35.5 7.0 37.0 82-83 29.704836283391096 34.0 29.5 35.0 2.0 37.0 84-85 29.360123524695 34.0 29.0 35.0 2.0 36.5 86-87 29.034205704402225 34.0 29.0 35.0 2.0 36.0 88-89 28.663894374457428 34.0 29.0 35.0 2.0 36.0 90-91 28.326317751373416 34.0 29.0 35.0 2.0 35.0 92-93 27.95445822095281 34.0 27.5 35.0 2.0 35.0 94-95 27.544785819842147 34.0 27.0 35.0 2.0 35.0 96-97 27.08709948906899 33.5 26.0 35.0 2.0 35.0 98-99 26.455150727960714 33.0 25.0 35.0 2.0 35.0 100-101 24.261833751930073 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5187198833807223 1101 2 0.4835781986332428 1101 3 0.5987231400200521 1101 4 0.46691955137262653 1101 5 0.3865963931831118 1101 6 0.34590179695052115 1101 7 0.40619507759871 1101 8 0.3454662113085689 1101 9 0.5210834668158597 1101 10-11 0.46895253872998666 1101 12-13 0.5531218924604957 1101 14-15 0.7793944253661849 1101 16-17 0.7588047970401561 1101 18-19 0.8091370789705437 1101 20-21 0.7605502116118572 1101 22-23 0.7627170837222437 1101 24-25 0.8733167663320813 1101 26-27 0.9204573164270329 1101 28-29 0.7858290309149893 1101 30-31 0.7564104384977455 1101 32-33 0.833002436253615 1101 34-35 0.8589878855699951 1101 36-37 0.7710710101029079 1101 38-39 0.8861810464286108 1101 40-41 0.913069628576622 1101 42-43 0.8433289518236933 1101 44-45 0.9820008431693807 1101 46-47 0.9869835446307391 1101 48-49 0.8801230282007353 1101 50-51 0.8871796261162359 1101 52-53 0.9803864273255911 1101 54-55 1.0188409297052985 1101 56-57 0.967419094284125 1101 58-59 0.9198616139264644 1101 60-61 1.0178248248415613 1101 62-63 1.0168312254752365 1101 64-65 0.9032457371030205 1101 66-67 0.8901752499479727 1101 68-69 0.8572059473662321 1101 70-71 0.8455331038888119 1101 72-73 0.9264023612407755 1101 74-75 0.9221413762515347 1101 76-77 0.8858133558739247 1101 78-79 1.0389037625438782 1101 80-81 1.0181329997009811 1101 82-83 0.81760808342732 1101 84-85 1.0943435392389773 1101 86-87 1.0713904502553113 1101 88-89 1.1736939264214747 1101 90-91 1.0547688638468884 1101 92-93 0.8589124405724817 1101 94-95 1.0209019083323234 1101 96-97 0.9978141114894186 1101 98-99 1.0788011181961359 1101 100-101 1.3174570191399333 1102 1 0.4299744497360436 1102 2 0.4740900894120692 1102 3 0.41838021742746534 1102 4 0.3316151311615343 1102 5 0.3135984440008315 1102 6 0.3802748997354968 1102 7 0.3667174623888556 1102 8 0.3158908328043921 1102 9 0.3385327335911512 1102 10-11 0.49881624175989714 1102 12-13 0.4777497514526914 1102 14-15 0.6360198392328869 1102 16-17 0.481360297425077 1102 18-19 0.5346367962789813 1102 20-21 0.5386990930503899 1102 22-23 0.5884801944559612 1102 24-25 0.6889060582679676 1102 26-27 0.5979348314952233 1102 28-29 0.7403406734259548 1102 30-31 0.6801919440024093 1102 32-33 0.7329768792677953 1102 34-35 0.6555963692375144 1102 36-37 0.719066612215066 1102 38-39 0.606315726528166 1102 40-41 0.6697780244115705 1102 42-43 0.6882874973495561 1102 44-45 0.6555264137160464 1102 46-47 0.5870434209975244 1102 48-49 0.5907403389382111 1102 50-51 0.4325444234023976 1102 52-53 0.6538290159040159 1102 54-55 0.6425535878195987 1102 56-57 0.8525016902897207 1102 58-59 0.7171370306096563 1102 60-61 0.7343989474024397 1102 62-63 0.8061704157047487 1102 64-65 0.9378446280341421 1102 66-67 0.6502317152394994 1102 68-69 0.7010769127526828 1102 70-71 0.7119702618685366 1102 72-73 0.9227726955261488 1102 74-75 0.8031801790580069 1102 76-77 0.752313467490147 1102 78-79 0.7906302151716602 1102 80-81 0.8445010286419219 1102 82-83 0.7407983358886163 1102 84-85 0.8078657404360889 1102 86-87 0.9254328873694817 1102 88-89 0.8472705587826681 1102 90-91 0.7699456451688 1102 92-93 0.7143444475156393 1102 94-95 0.8002920769249791 1102 96-97 0.6977423954577802 1102 98-99 0.7922874874559902 1102 100-101 1.2383128234965106 1103 1 0.3907677401037013 1103 2 0.5225287958368732 1103 3 0.5368347829110895 1103 4 0.4630671778534605 1103 5 0.4469750142759139 1103 6 0.3690311446120731 1103 7 0.3566479524714907 1103 8 0.2932739094618029 1103 9 0.3843894304211233 1103 10-11 0.46711122422362905 1103 12-13 0.2520208599790692 1103 14-15 0.41149377499873196 1103 16-17 0.7169490476161187 1103 18-19 0.8198353719409894 1103 20-21 0.5703163796229873 1103 22-23 0.4974835349811215 1103 24-25 0.7923313867132364 1103 26-27 0.7998002061481202 1103 28-29 0.7486132368572314 1103 30-31 0.8072968916714842 1103 32-33 0.7816686557801091 1103 34-35 0.7380589595239897 1103 36-37 0.7712345792980884 1103 38-39 0.8039510098574922 1103 40-41 0.9825063085169745 1103 42-43 0.9994021867283536 1103 44-45 0.899117091209483 1103 46-47 0.8469654555619357 1103 48-49 0.9095939612147319 1103 50-51 0.944509378653656 1103 52-53 0.975746522994946 1103 54-55 1.0879708855074242 1103 56-57 1.0439203813516826 1103 58-59 1.032488063631824 1103 60-61 1.0222114393389319 1103 62-63 1.126880579991898 1103 64-65 1.0960150702418758 1103 66-67 1.1239683496653683 1103 68-69 1.0410880379122105 1103 70-71 0.9658505210123067 1103 72-73 1.0290215914458685 1103 74-75 0.9630555693317753 1103 76-77 0.9418855904802257 1103 78-79 1.0665995232284189 1103 80-81 1.0332453643379012 1103 82-83 0.9965982557001318 1103 84-85 1.065049904004713 1103 86-87 1.1996563095519193 1103 88-89 1.2805334460047142 1103 90-91 1.1934098074671127 1103 92-93 1.0398683522183738 1103 94-95 1.1681905332966878 1103 96-97 0.9929655735020155 1103 98-99 1.1007819089973125 1103 100-101 1.4920342935657622 1104 1 0.46235603077410303 1104 2 0.4206809650660297 1104 3 0.42014735573641815 1104 4 0.38274267858894717 1104 5 0.3743572237858004 1104 6 0.3620253249197489 1104 7 0.32090260928584513 1104 8 0.40062539425160537 1104 9 0.4734688529683737 1104 10-11 0.607539433574388 1104 12-13 0.5625640533364518 1104 14-15 0.5363316410136534 1104 16-17 0.6256895557899753 1104 18-19 0.6037446842068945 1104 20-21 0.6360875692332044 1104 22-23 0.5943701977711271 1104 24-25 0.6722058313068686 1104 26-27 0.6419268358735906 1104 28-29 0.6915910682048718 1104 30-31 0.6448087191147565 1104 32-33 0.6976040020013698 1104 34-35 0.7015411315757021 1104 36-37 0.7395405823079884 1104 38-39 0.7237826582770239 1104 40-41 0.7456064225997991 1104 42-43 0.7973570647503294 1104 44-45 0.7136269827861739 1104 46-47 0.723794444480248 1104 48-49 0.6933569630047245 1104 50-51 0.7374761683350002 1104 52-53 0.9450055355331131 1104 54-55 0.7818876423804895 1104 56-57 0.8222467660970807 1104 58-59 0.8665720799153362 1104 60-61 0.8737211340754811 1104 62-63 0.843011576286095 1104 64-65 0.8973897936843613 1104 66-67 0.7316876500661635 1104 68-69 0.8581951443785485 1104 70-71 0.8504195212534853 1104 72-73 0.829761263121128 1104 74-75 0.7024039603436201 1104 76-77 0.8438407763828231 1104 78-79 0.937810218663369 1104 80-81 1.081568561827872 1104 82-83 0.8572948471219135 1104 84-85 0.9563529770621564 1104 86-87 1.009172066503968 1104 88-89 0.938132334179457 1104 90-91 0.9448179918131281 1104 92-93 1.004560452882668 1104 94-95 1.0273298226932432 1104 96-97 0.8267342232890584 1104 98-99 0.8188117263908978 1104 100-101 1.0087388806703075 1105 1 0.32047821348821515 1105 2 0.35663013959543477 1105 3 0.3985616495834208 1105 4 0.44214977698280933 1105 5 0.38910510294698497 1105 6 0.3449477428159895 1105 7 0.36917644714083764 1105 8 0.3102587154525267 1105 9 0.4314781109314296 1105 10-11 0.4425442865388973 1105 12-13 0.4059392901517853 1105 14-15 0.4201956740138115 1105 16-17 0.5117849258751832 1105 18-19 0.48783116030137563 1105 20-21 0.627412661859502 1105 22-23 0.6150136821996455 1105 24-25 0.6232218274244943 1105 26-27 0.5638516429215912 1105 28-29 0.5320642789075265 1105 30-31 0.40366038638804724 1105 32-33 0.5280309228887177 1105 34-35 0.37895865451062605 1105 36-37 0.4201233580170296 1105 38-39 0.6526040878483741 1105 40-41 0.6532242703100337 1105 42-43 0.7058400933734887 1105 44-45 0.5435663673929483 1105 46-47 0.6618561984536129 1105 48-49 0.7240591963016598 1105 50-51 0.6193375135394419 1105 52-53 0.697295528160339 1105 54-55 0.8785485694833639 1105 56-57 0.8924534370567585 1105 58-59 0.9006431105170023 1105 60-61 0.8260434175819427 1105 62-63 0.8900084778549129 1105 64-65 0.9815118737396062 1105 66-67 1.0856196712325499 1105 68-69 0.9495916782419656 1105 70-71 0.9534844483190312 1105 72-73 0.9031017825749785 1105 74-75 0.9241635799458656 1105 76-77 0.6991663916360658 1105 78-79 0.9110789702382505 1105 80-81 0.9136990599100159 1105 82-83 0.9599239873072598 1105 84-85 0.9345439878786657 1105 86-87 0.9551530186182724 1105 88-89 0.9205401978484886 1105 90-91 0.806917272267814 1105 92-93 0.8599746347381831 1105 94-95 0.9033851091581404 1105 96-97 0.9579373385189669 1105 98-99 1.0035912898107462 1105 100-101 1.1344052766908774 1106 1 0.5803416335487803 1106 2 0.5728246475574501 1106 3 0.5862198083292718 1106 4 0.46079736777859637 1106 5 0.5078709394218635 1106 6 0.6286619755580887 1106 7 0.6849816095741446 1106 8 0.5989891194242674 1106 9 0.7883277081957161 1106 10-11 0.8244219002010098 1106 12-13 0.7935955948713129 1106 14-15 0.9964559999104381 1106 16-17 0.8829462673662292 1106 18-19 0.9366651837445019 1106 20-21 0.9061310146601116 1106 22-23 0.9415754933773641 1106 24-25 0.9470214483791111 1106 26-27 1.0297562017663253 1106 28-29 0.9773048903242056 1106 30-31 0.9430482486956961 1106 32-33 0.9523614133816807 1106 34-35 0.9920492133965624 1106 36-37 0.9953891017906003 1106 38-39 1.1647946199539305 1106 40-41 1.0801987053470157 1106 42-43 1.081147655740871 1106 44-45 1.165552956602511 1106 46-47 1.0024673061313365 1106 48-49 1.024816772059232 1106 50-51 0.8301871726045889 1106 52-53 0.8731164498174238 1106 54-55 1.010379214478526 1106 56-57 1.144024312363186 1106 58-59 1.1362994358368255 1106 60-61 1.2270177084168665 1106 62-63 1.0996340675382825 1106 64-65 1.016472733183818 1106 66-67 1.2668031580380656 1106 68-69 1.1206330230290646 1106 70-71 1.2028472812712536 1106 72-73 1.0744976583964174 1106 74-75 1.1053607659459068 1106 76-77 1.2208835290194138 1106 78-79 1.2290092588483468 1106 80-81 1.3339873610038921 1106 82-83 1.2902487509189022 1106 84-85 1.392205545672784 1106 86-87 1.4539867330570182 1106 88-89 1.4956467013608368 1106 90-91 1.4754451753640403 1106 92-93 1.5679118244462735 1106 94-95 1.6496080455238342 1106 96-97 1.5433886909281682 1106 98-99 1.6252350901527102 1106 100-101 1.9414106121666386 1107 1 0.33170791251489007 1107 2 0.45826665283022194 1107 3 0.44418720473381157 1107 4 0.39671308983985654 1107 5 0.33187669420669863 1107 6 0.3285633496713345 1107 7 0.3683558062985952 1107 8 0.3991074620982502 1107 9 0.5305407833202409 1107 10-11 0.5510282467831189 1107 12-13 0.5364171636102171 1107 14-15 0.39752922239487987 1107 16-17 0.4140923412819504 1107 18-19 0.5606505295570301 1107 20-21 0.5804488085781543 1107 22-23 0.6030139252970841 1107 24-25 0.634213150716036 1107 26-27 0.5926867854608346 1107 28-29 0.6194471947677656 1107 30-31 0.645933674654863 1107 32-33 0.6558980701403101 1107 34-35 0.7626749130540986 1107 36-37 0.6819498144979192 1107 38-39 0.8179670510191173 1107 40-41 0.9008238701342606 1107 42-43 0.9053661001241124 1107 44-45 0.9802067140872808 1107 46-47 0.9067182874085731 1107 48-49 0.8590500333983258 1107 50-51 0.7873975492560632 1107 52-53 0.7987291017861367 1107 54-55 0.8270030775383077 1107 56-57 0.8619527030895 1107 58-59 0.8273423615899063 1107 60-61 0.8986000078599119 1107 62-63 0.9226135006219209 1107 64-65 1.0405256928938158 1107 66-67 0.929644523270369 1107 68-69 0.9191933724440915 1107 70-71 0.97013166719087 1107 72-73 0.9217452053868698 1107 74-75 0.8729701117869588 1107 76-77 0.7639332985951945 1107 78-79 0.9618988630883791 1107 80-81 1.0639861486312299 1107 82-83 1.0935860164228508 1107 84-85 1.015243594247817 1107 86-87 1.0382370368318838 1107 88-89 1.0902506781529198 1107 90-91 0.999596299504077 1107 92-93 1.0570850009738173 1107 94-95 1.0001463033275364 1107 96-97 0.9418028214881176 1107 98-99 1.156532815527651 1107 100-101 1.2778108847617098 1108 1 -0.16621309639344162 1108 2 0.14763590773256752 1108 3 -0.02888682984897528 1108 4 -0.04564533228683132 1108 5 0.02080413075861287 1108 6 -0.032397830883475365 1108 7 -0.0011875141882740081 1108 8 -0.1651993504613003 1108 9 -0.22113515307593445 1108 10-11 -0.17695052755377105 1108 12-13 -0.27823489134142676 1108 14-15 0.010537293966692118 1108 16-17 0.012370269932382882 1108 18-19 0.06429959802986218 1108 20-21 0.09109573928252956 1108 22-23 0.01803342385209561 1108 24-25 0.16643532973987618 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-2.04036925590907 2316 90-91 -2.0071249632934105 2316 92-93 -2.22704021094755 2316 94-95 -2.3813244565640836 2316 96-97 -2.3044270339086985 2316 98-99 -2.3180617959562646 2316 100-101 -2.042110341343726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9016.0 3 3020.0 4 3158.0 5 3331.0 6 3813.0 7 4567.0 8 5458.0 9 6713.0 10 8427.0 11 10127.0 12 12451.0 13 14881.0 14 16242.0 15 19389.0 16 24137.0 17 30009.0 18 26536.0 19 19027.0 20 18609.0 21 20140.0 22 21988.0 23 24478.0 24 28442.0 25 32433.0 26 37309.0 27 44498.0 28 52922.0 29 63831.0 30 77252.0 31 94559.0 32 117680.0 33 151549.0 34 200843.0 35 278777.0 36 407704.0 37 571488.0 38 475367.0 39 77817.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71731463289944 18.99403167766153 14.663069273251825 22.62558441618721 2 15.092038009852368 20.587686099990282 40.38326258418839 23.937013305968964 3 18.854784909514116 25.54235163829519 29.879904772641364 25.72295867954933 4 12.733397120805156 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22.49328661412441 26.337691556741312 29.152149800301743 22.01687202883254 26-27 22.3170320538896 26.513026005598086 29.014891646932806 22.15505029357951 28-29 22.095325312358153 26.537669483081782 29.216540799447216 22.15046440511285 30-31 22.29983713079693 26.254379616851274 29.1500402283285 22.29574302402329 32-33 22.19060383639607 26.391209897203044 29.09701846062146 22.321167805779424 34-35 22.54783533129951 26.409153727096708 28.84173542116738 22.201275520436404 36-37 22.56401518177789 26.246895834293326 28.82293484094704 22.366154142981742 38-39 22.367761960755324 26.455000495605685 28.933625635709596 22.24361190792939 40-41 22.579360638847238 26.3008911468034 28.908981344022784 22.210766870326577 42-43 22.35271709002947 26.40615509480904 28.67764660323961 22.563481211921882 44-45 22.51234755546745 26.53427430783577 28.422052192061965 22.531325944634816 46-47 22.664887593383533 26.725742241317445 28.065400333750258 22.543969831548765 48-49 22.714333424768558 26.888553165993056 27.773441740705717 22.623671668532662 50-51 22.619028877284673 26.933295903468068 27.749582932644305 22.698092286602954 52-53 22.838308355464925 27.076750760075896 27.4448058276242 22.640135056834982 54-55 22.665738035979246 26.948798459737162 27.494440812722022 22.89102269156157 56-57 22.623302601714606 27.352903198964757 27.39352986739727 22.63026433192336 58-59 22.85756816647725 26.87491794561004 27.46690307171852 22.80061081619419 60-61 22.820235743195767 26.753880609360476 27.85546759678959 22.57041605065417 62-63 22.63983608568369 26.918865065761338 27.835528693293902 22.605770155261073 64-65 22.6295249130324 26.90881632413585 27.69783522820407 22.76382353462768 66-67 22.580107727594935 27.262770189309066 27.349968137935882 22.807153945160117 68-69 22.457705643678814 27.019174060367607 27.66987941661951 22.853240879334074 70-71 22.504903069607433 27.03456881573158 27.587192610471316 22.873335504189672 72-73 22.696221726246453 26.96927632811929 27.390052981104734 22.94444896452952 74-75 22.5347344096617 27.293528341203448 27.264957944840575 22.906779304294275 76-77 22.702800626048226 27.047978412175823 27.32037867199611 22.928842289779844 78-79 22.635985399935215 27.28323283549133 27.17092081928259 22.90986094529087 80-81 22.55201524260079 27.30700793686572 27.125673891079575 23.015302929453913 82-83 22.56780112531915 27.29217219882826 27.241280533755763 22.898746142096822 84-85 22.678467639607582 27.15220655692374 27.106020764044974 23.0633050394237 86-87 22.674256582227002 27.02482527052627 27.28367638206583 23.017241765180895 88-89 22.745356198865124 27.026640710242066 27.099983086316165 23.128020004576644 90-91 22.576263802283563 27.076828608818676 27.380915672908877 22.96599191598888 92-93 22.786873341211955 26.9357335275135 27.21382300159562 23.063570129678926 94-95 22.65690856647519 26.90595666589069 27.22929777890969 23.20783698872443 96-97 22.63379426181107 26.855013399366456 27.24642314603958 23.264769192782897 98-99 22.432349091410938 26.805977408145427 27.21988550306512 23.54178799737851 100-101 22.470584488673175 26.861970601241143 27.271278206762624 23.39616670332306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 743.0 1 536.5 2 403.5 3 502.0 4 472.0 5 511.5 6 778.5 7 1481.0 8 2606.5 9 3280.0 10 3102.5 11 2855.0 12 3020.0 13 3305.5 14 3550.5 15 3784.5 16 3996.0 17 4214.0 18 4429.5 19 4719.0 20 5190.0 21 5914.0 22 6915.0 23 8318.0 24 10126.5 25 12782.5 26 16722.5 27 20597.0 28 23327.5 29 27028.5 30 32804.5 31 38935.5 32 44405.0 33 49988.0 34 57640.0 35 63639.0 36 69372.5 37 76727.0 38 81325.0 39 82604.0 40 84055.0 41 89211.0 42 93333.0 43 97844.0 44 103920.0 45 105933.5 46 105807.5 47 108044.5 48 115116.5 49 118513.0 50 115312.5 51 112247.5 52 108667.0 53 106669.0 54 105047.5 55 99094.5 56 90993.0 57 82972.0 58 76503.5 59 69966.5 60 60375.5 61 48279.5 62 37140.0 63 28583.5 64 21420.5 65 15574.5 66 11006.5 67 8258.0 68 5925.5 69 3599.5 70 2170.0 71 1337.5 72 841.5 73 608.0 74 434.0 75 287.0 76 194.0 77 127.0 78 73.5 79 42.5 80 41.0 81 34.0 82 20.5 83 14.0 84 15.0 85 13.5 86 12.5 87 13.5 88 7.5 89 5.5 90 8.0 91 6.0 92 4.0 93 4.0 94 3.5 95 2.5 96 1.0 97 1.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05838264822632057 2 0.09632142928144942 3 0.0689193577246009 4 0.1273682745641182 5 0.10294829060112258 6 0.09989993439407294 7 0.10891246578882843 8 0.09542680300329355 9 0.1165002219998542 10-11 0.10823321250356194 12-13 0.0910199402257109 14-15 0.09367068475358016 16-17 0.10723918330561096 18-19 0.08631486868874295 20-21 0.07084114750730611 22-23 0.07480069714581082 24-25 0.06111622852068574 26-27 0.05404205406193465 28-29 0.06663309056931366 30-31 0.049336982524966704 32-33 0.049403251138163434 34-35 0.0453608657331628 36-37 0.04999966865693402 38-39 0.04999966865693402 40-41 0.06502607669929292 42-43 0.06136473582017349 44-45 0.04741519274226148 46-47 0.03623236426531302 48-49 0.04348877741035513 50-51 0.027219832870557514 52-53 0.04029131682361283 54-55 0.046122954784925216 56-57 0.050645787635602145 58-59 0.057256081801976125 60-61 0.05448936720101259 62-63 0.05997309494304213 64-65 0.040937435802280965 66-67 0.06275637669730486 68-69 0.053048024863983675 70-71 0.02576192338022942 72-73 0.020659240164081084 74-75 0.03018535331111126 76-77 0.04372071755654369 78-79 0.06192801903234571 80-81 0.04015877959721936 82-83 0.059939960636443766 84-85 0.06431368910742806 86-87 0.06628518035003082 88-89 0.08979397088157136 90-91 0.06437995772062478 92-93 0.07501607013870021 94-95 0.08197427452435703 96-97 0.09163292489778066 98-99 0.1227129044870478 100-101 0.11726231105161661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3018020.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.47404212646086 #Duplication Level Percentage of deduplicated Percentage of total 1 70.0540915106457 31.856427084860467 2 14.78248599781371 13.444387819967968 3 5.7769522262996436 7.881041067039055 4 2.89143390676902 5.259407491291668 5 1.6278344615317142 3.701210643929895 6 1.0444674244928795 2.8497693396663166 7 0.7041008646706618 2.2412818666917835 8 0.5043827958938121 1.8349059606669855 9 0.3780477619325639 1.547222386674209 >10 2.006338430821886 16.5279840096154 >50 0.14761551335860737 4.632708754625452 >100 0.07903436973500111 6.249098555653835 >500 0.002630238574031429 0.7886126422511993 >1k 3.6531091300835266E-4 0.2454613330091485 >5k 1.4612436520334106E-4 0.49677014769258093 >10k+ 7.306218260167053E-5 0.4437108963639927 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13355 0.4425086646211755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8929 0.2958562236168084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6023 0.19956792864195733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 3.3134306598365814E-5 10-11 0.0 0.0 0.0 0.0 3.3134306598365814E-5 12-13 0.0 0.0 0.0 1.6567153299182907E-5 3.3134306598365814E-5 14-15 0.0 0.0 0.0 3.3134306598365814E-5 3.3134306598365814E-5 16-17 0.0 0.0 0.0 6.626861319673163E-5 3.3134306598365814E-5 18-19 0.0 1.6567153299182907E-5 0.0 9.940291979509745E-5 3.3134306598365814E-5 20-21 0.0 3.3134306598365814E-5 0.0 1.1597007309428035E-4 3.3134306598365814E-5 22-23 0.0 3.3134306598365814E-5 0.0 1.82238686291012E-4 6.626861319673163E-5 24-25 0.0 3.3134306598365814E-5 0.0 3.1477591268447524E-4 1.1597007309428035E-4 26-27 0.0 3.3134306598365814E-5 0.0 8.117905116599624E-4 1.3253722639346326E-4 28-29 0.0 3.3134306598365814E-5 0.0 0.0018389540162093028 1.3253722639346326E-4 30-31 0.0 3.3134306598365814E-5 0.0 0.004589101463873666 1.3253722639346326E-4 32-33 0.0 3.3134306598365814E-5 0.0 0.007985367890206162 1.3253722639346326E-4 34-35 0.0 3.3134306598365814E-5 0.0 0.011331932856641108 1.4910437969264617E-4 36-37 0.0 3.3134306598365814E-5 0.0 0.012955513879961033 1.6567153299182908E-4 38-39 0.0 3.3134306598365814E-5 0.0 0.015308049648445007 1.6567153299182908E-4 40-41 0.0 3.3134306598365814E-5 0.0 0.01789252556311754 1.6567153299182908E-4 42-43 0.0 3.3134306598365814E-5 0.0 0.020742075930577003 1.82238686291012E-4 44-45 0.0 3.3134306598365814E-5 0.0 0.024055506590413583 1.988058395901949E-4 46-47 9.940291979509745E-5 3.3134306598365814E-5 0.0 0.028097891995414213 2.650744527869265E-4 48-49 9.940291979509745E-5 4.970145989754872E-5 0.0 0.0334325153577511 3.3134306598365816E-4 50-51 9.940291979509745E-5 6.626861319673163E-5 0.0 0.04150071901445318 3.3134306598365816E-4 52-53 9.940291979509745E-5 6.626861319673163E-5 0.0 0.05861458837250913 3.64477372582024E-4 54-55 9.940291979509745E-5 6.626861319673163E-5 0.0 0.0801684548147461 3.976116791803898E-4 56-57 9.940291979509745E-5 6.626861319673163E-5 0.0 0.10583097527518041 3.976116791803898E-4 58-59 9.940291979509745E-5 6.626861319673163E-5 0.0 0.14398512932319865 4.141788324795727E-4 60-61 9.940291979509745E-5 9.940291979509745E-5 0.0 0.19391852936693593 4.307459857787556E-4 62-63 9.940291979509745E-5 9.940291979509745E-5 0.0 0.23076387830431871 4.6388029237712144E-4 64-65 9.940291979509745E-5 9.940291979509745E-5 0.0 0.26673116811684483 4.6388029237712144E-4 66-67 9.940291979509745E-5 9.940291979509745E-5 0.0 0.3343085864242119 4.6388029237712144E-4 68-69 1.3253722639346326E-4 9.940291979509745E-5 0.0 0.42198196168348784 4.6388029237712144E-4 70-71 1.4910437969264617E-4 9.940291979509745E-5 0.0 0.4919616172192364 5.30148905573853E-4 72-73 1.6567153299182908E-4 9.940291979509745E-5 0.0 0.5547511282231397 6.626861319673163E-4 74-75 1.6567153299182908E-4 9.940291979509745E-5 0.0 0.6759564217599618 6.626861319673163E-4 76-77 1.6567153299182908E-4 9.940291979509745E-5 0.0 0.7620062159959179 6.626861319673163E-4 78-79 1.6567153299182908E-4 9.940291979509745E-5 0.0 0.8286061722586331 6.626861319673163E-4 80-81 1.988058395901949E-4 9.940291979509745E-5 0.0 0.9137944745230316 7.12387591864865E-4 82-83 1.988058395901949E-4 9.940291979509745E-5 0.0 1.0796482462011516 7.952233583607796E-4 84-85 1.988058395901949E-4 9.940291979509745E-5 0.0 1.2494781346710757 7.952233583607796E-4 86-87 1.988058395901949E-4 1.3253722639346326E-4 0.0 1.4612560552945308 7.952233583607796E-4 88-89 1.988058395901949E-4 1.3253722639346326E-4 0.0 1.7343655774315612 7.952233583607796E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8025 0.0 43.050373 1 GTATCAA 13860 0.0 40.423893 1 TATCAAC 17700 0.0 31.304941 2 ATCAACG 17795 0.0 31.164001 3 TCAACGC 17855 0.0 31.059792 4 CAACGCA 18095 0.0 30.673082 5 AACGCAG 18575 0.0 29.85487 6 GTACATG 15550 0.0 26.098469 1 ACGCAGA 21205 0.0 25.860723 7 CGCAGAG 21395 0.0 25.429483 8 TACATGG 15795 0.0 25.091925 2 GACGCGT 120 1.7076911E-4 23.758244 8 ACATGGG 16065 0.0 23.72324 3 GCAGAGT 22400 0.0 23.482868 9 GAGTACT 10580 0.0 22.318111 12-13 AGAGTAC 18920 0.0 20.956074 10-11 CATGGGG 7900 0.0 20.572477 4 ATTCGCG 420 0.0 20.355768 80-81 AGTACTT 11175 0.0 20.343287 12-13 GTACTTT 11830 0.0 20.160704 14-15 >>END_MODULE