FastQCFastQC Report
Thu 26 May 2016
SRR937139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018020
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138570.4591420865335551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102930.34105141781697934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68410.22667179143942054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79650.044.4955641
GTATCAA145050.042.0216031
TATCAAC187650.032.4011422
ATCAACG187550.032.2142033
TCAACGC188750.032.008874
CAACGCA191650.031.5250385
AACGCAG197400.030.6543736
ACGCAGA225800.026.5884657
CGCAGAG228150.026.1272328
GCAGAGT243700.023.9143839
GTACATG149550.023.6347061
GAGTACT123700.023.11477712-13
TACATGG154550.022.3749082
AGTACTT130150.021.96925212-13
ACATGGG154450.021.680963
AGAGTAC208750.021.35393710-11
GTACTTT134950.021.24150714-15
CAGAGTA236450.019.22395510-11
TACTTTT156250.018.37626314-15
CATGGGA89300.018.2968854
Produced by FastQC (version 0.11.5)