##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937139_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3018020 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.993758490666067 31.0 31.0 34.0 30.0 34.0 2 31.98346564966435 31.0 31.0 34.0 30.0 34.0 3 32.16747702135837 33.0 31.0 34.0 30.0 34.0 4 35.93299944997051 37.0 35.0 37.0 35.0 37.0 5 35.842892359891586 37.0 35.0 37.0 35.0 37.0 6 35.87777913996594 37.0 35.0 37.0 35.0 37.0 7 35.806044360209675 37.0 35.0 37.0 33.0 37.0 8 35.78483111443927 37.0 35.0 37.0 33.0 37.0 9 37.43866309699737 39.0 37.0 39.0 34.0 39.0 10-11 37.35849215710963 39.0 37.0 39.0 34.0 39.0 12-13 37.2585562719929 39.0 37.0 39.0 33.5 39.0 14-15 38.427881193630256 40.0 38.0 41.0 33.5 41.0 16-17 38.346146645814144 40.0 38.0 41.0 33.0 41.0 18-19 38.40434854639797 40.0 38.0 41.0 33.5 41.0 20-21 38.48290402316751 40.0 38.0 41.0 34.0 41.0 22-23 38.411373516411416 40.0 38.0 41.0 34.0 41.0 24-25 38.30957863102299 40.0 38.0 41.0 34.0 41.0 26-27 38.14586782062412 40.0 38.0 41.0 33.0 41.0 28-29 37.96531268845137 40.0 37.0 41.0 33.0 41.0 30-31 37.82378960377996 40.0 37.0 41.0 33.0 41.0 32-33 37.80928754613952 40.0 37.0 41.0 33.0 41.0 34-35 37.72369252026163 40.0 37.0 41.0 33.0 41.0 36-37 37.56623796396313 40.0 37.0 41.0 33.0 41.0 38-39 37.41157099687875 40.0 36.5 41.0 32.0 41.0 40-41 37.229365279222804 40.0 36.0 41.0 31.5 41.0 42-43 36.99921620797741 39.0 36.0 41.0 31.0 41.0 44-45 36.66162351475471 39.0 36.0 41.0 30.5 41.0 46-47 36.372387359924716 39.0 35.0 41.0 30.0 41.0 48-49 36.040352449619284 39.0 35.0 40.5 29.5 41.0 50-51 35.76717980662819 39.0 35.0 40.0 28.5 41.0 52-53 35.499723659882974 38.5 35.0 40.0 28.0 41.0 54-55 35.25030715502217 38.0 34.5 40.0 27.0 41.0 56-57 34.96863224895793 38.0 34.0 40.0 26.5 41.0 58-59 34.62140128296035 38.0 34.0 40.0 26.0 41.0 60-61 34.28130446451647 37.0 33.5 40.0 26.0 41.0 62-63 33.90283662798789 37.0 33.0 39.5 25.0 41.0 64-65 33.48943562335571 36.0 33.0 39.0 24.0 40.5 66-67 33.100862817343824 36.0 32.0 39.0 23.0 40.0 68-69 32.665135916925664 35.0 32.0 38.5 22.0 40.0 70-71 32.19405785912618 35.0 31.0 38.0 21.5 40.0 72-73 31.84518906435345 35.0 31.0 37.0 20.5 39.0 74-75 31.39948161377327 35.0 31.0 36.5 19.5 39.0 76-77 30.004656198434734 33.5 29.5 35.0 18.0 37.5 78-79 30.74008058263365 34.0 30.0 36.0 18.5 38.0 80-81 30.584426378884167 34.0 30.0 35.0 18.0 37.0 82-83 30.259380156526465 34.0 30.0 35.0 16.0 37.0 84-85 29.88749759776277 34.0 30.0 35.0 11.0 36.0 86-87 29.526512084081617 34.0 29.5 35.0 7.0 36.0 88-89 29.174984261204365 34.0 29.0 35.0 4.0 35.5 90-91 28.74879159183836 34.0 29.0 35.0 2.0 35.0 92-93 28.27981226101881 33.5 29.0 35.0 2.0 35.0 94-95 27.782610287539512 33.0 27.0 35.0 2.0 35.0 96-97 27.140081742334377 33.0 26.5 35.0 2.0 35.0 98-99 26.373647623276185 33.0 25.0 34.0 2.0 35.0 100-101 23.36781350024188 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39598957650944655 1101 2 0.2509733307502806 1101 3 0.31729011997954615 1101 4 0.2692670355413611 1101 5 0.28224619014699925 1101 6 0.2908549643287941 1101 7 0.3240028808345983 1101 8 0.3562014981384749 1101 9 0.44351199421301146 1101 10-11 0.4634207480507726 1101 12-13 0.432881631888975 1101 14-15 0.519276423011469 1101 16-17 0.5868261441349176 1101 18-19 0.5590309891729319 1101 20-21 0.5856037342274263 1101 22-23 0.5274224356203661 1101 24-25 0.573903250174439 1101 26-27 0.569325086346609 1101 28-29 0.5490010039633617 1101 30-31 0.6071496372903837 1101 32-33 0.6316119836230243 1101 34-35 0.638715657237789 1101 36-37 0.6807937838326907 1101 38-39 0.5779620907486134 1101 40-41 0.524994336549959 1101 42-43 0.5905008303249346 1101 44-45 0.7000010423409648 1101 46-47 0.6663145956078367 1101 48-49 0.49884939865494715 1101 50-51 0.47496985855062235 1101 52-53 0.3234452036398068 1101 54-55 0.504085715666001 1101 56-57 0.5938346027926471 1101 58-59 0.542326741511026 1101 60-61 0.5826243881380577 1101 62-63 0.6393784840817531 1101 64-65 0.6434378312924025 1101 66-67 0.73813439100919 1101 68-69 0.6131349623854376 1101 70-71 0.5808550344642072 1101 72-73 0.568136668035109 1101 74-75 0.5813683663374611 1101 76-77 0.472491493412285 1101 78-79 0.640647163411419 1101 80-81 0.7854976907954487 1101 82-83 0.8859793625843402 1101 84-85 0.8185930363130289 1101 86-87 0.5769388538025773 1101 88-89 0.7215485374570072 1101 90-91 0.8060788868409787 1101 92-93 0.7902756481866682 1101 94-95 0.9526323788611109 1101 96-97 0.9356671811613353 1101 98-99 0.9136172135828993 1101 100-101 1.2280542446120926 1102 1 0.28783752391838036 1102 2 0.1471139129488499 1102 3 0.20611514538006048 1102 4 0.19325816048685596 1102 5 0.24455777257297484 1102 6 0.24034851875265417 1102 7 0.2889439120016064 1102 8 0.31928698782075315 1102 9 0.4134576047746208 1102 10-11 0.38196494911853307 1102 12-13 0.3738829128676926 1102 14-15 0.4514015541866101 1102 16-17 0.45251698597159873 1102 18-19 0.44171675716557246 1102 20-21 0.46960266187716826 1102 22-23 0.39344619631518185 1102 24-25 0.40802579018416196 1102 26-27 0.43376332293544806 1102 28-29 0.3486701713897773 1102 30-31 0.4310984628164931 1102 32-33 0.575416187903663 1102 34-35 0.5803074089031739 1102 36-37 0.5513857253499523 1102 38-39 0.5775842124582766 1102 40-41 0.5887713252485511 1102 42-43 0.6445713279663892 1102 44-45 0.6108792951193678 1102 46-47 0.49269660265530746 1102 48-49 0.4191759126194867 1102 50-51 0.5524584309269613 1102 52-53 0.6332780713839696 1102 54-55 0.4927987597429322 1102 56-57 0.5654189377164229 1102 58-59 0.5366344184123051 1102 60-61 0.6066003875205155 1102 62-63 0.750000697587943 1102 64-65 0.5741168021699536 1102 66-67 0.6755934215962114 1102 68-69 0.41401965135170826 1102 70-71 0.4243835362406827 1102 72-73 0.6376755574223836 1102 74-75 0.37803224085996234 1102 76-77 0.5872805909064454 1102 78-79 0.5266252449058655 1102 80-81 0.5886591942555519 1102 82-83 0.5540061129249239 1102 84-85 0.485655349460167 1102 86-87 0.33062651822787714 1102 88-89 0.5223363706627282 1102 90-91 0.8029610388852255 1102 92-93 0.608140607159882 1102 94-95 0.7855551487793875 1102 96-97 0.7512034293123655 1102 98-99 0.8076221705950068 1102 100-101 0.8550715774641908 1103 1 0.11108970280221442 1103 2 -0.1569651798891698 1103 3 0.019854568539052764 1103 4 0.09922019401913929 1103 5 0.12882246999046743 1103 6 0.16459699983591491 1103 7 0.25704219449927734 1103 8 0.30903457273817025 1103 9 0.27213751180362067 1103 10-11 0.31013308888466895 1103 12-13 0.30619256942677 1103 14-15 0.3456203417129373 1103 16-17 0.4271942967992288 1103 18-19 0.35445476710179236 1103 20-21 0.346619035015614 1103 22-23 0.39452422526640163 1103 24-25 0.42344847677620834 1103 26-27 0.4067003055063694 1103 28-29 0.4197522494623982 1103 30-31 0.45935264641239115 1103 32-33 0.5964452701226648 1103 34-35 0.5623044990933792 1103 36-37 0.5523860965194203 1103 38-39 0.5937184229689265 1103 40-41 0.5746124012033178 1103 42-43 0.5425014627584588 1103 44-45 0.6835193917425144 1103 46-47 0.620206710572333 1103 48-49 0.6644817602256481 1103 50-51 0.495728652219384 1103 52-53 0.6466526766442584 1103 54-55 0.64969521735717 1103 56-57 0.5983347715674725 1103 58-59 0.7546773232202426 1103 60-61 0.7230838843735157 1103 62-63 0.6883237460206004 1103 64-65 0.5230518733173852 1103 66-67 0.6817069994514853 1103 68-69 0.6675342490298206 1103 70-71 0.5322399730535423 1103 72-73 0.6727434298274275 1103 74-75 0.685835086360175 1103 76-77 0.5736054277010503 1103 78-79 0.6555030229703469 1103 80-81 0.710237488186042 1103 82-83 0.7023622490309833 1103 84-85 0.6873558315834956 1103 86-87 0.6280141898441336 1103 88-89 0.8453827688303015 1103 90-91 1.095329139748042 1103 92-93 1.1056385875613408 1103 94-95 1.0354020661872383 1103 96-97 1.278778243777257 1103 98-99 1.233709726890691 1103 100-101 1.2837584612881479 1104 1 0.2691283381731857 1104 2 0.13521660058407647 1104 3 0.14917968903525036 1104 4 0.09051824770111949 1104 5 0.12139293055319911 1104 6 0.16657576624617576 1104 7 0.23600822810720956 1104 8 0.26825526623159135 1104 9 0.21120584469819192 1104 10-11 0.2619080715303923 1104 12-13 0.2789352492722941 1104 14-15 0.3544676867656307 1104 16-17 0.3777037826030778 1104 18-19 0.3576456694981687 1104 20-21 0.2309095223009976 1104 22-23 0.2424981874721368 1104 24-25 0.25749477576418656 1104 26-27 0.1839744928737943 1104 28-29 0.29370954139129424 1104 30-31 0.30816867363333955 1104 32-33 0.33630409227006197 1104 34-35 0.23968961177931902 1104 36-37 0.2567658998961875 1104 38-39 0.36182380451680274 1104 40-41 0.4353090406114788 1104 42-43 0.36874028157177463 1104 44-45 0.41366457069957363 1104 46-47 0.4261613981843482 1104 48-49 0.34491986244872663 1104 50-51 0.3336526346897699 1104 52-53 0.4148126187074226 1104 54-55 0.4357023022584201 1104 56-57 0.4044301072624421 1104 58-59 0.3454428432395318 1104 60-61 0.4268385691639338 1104 62-63 0.5352067378524552 1104 64-65 0.4798612031750906 1104 66-67 0.5784097400400796 1104 68-69 0.5787917388641404 1104 70-71 0.5151491915540589 1104 72-73 0.41319190862555644 1104 74-75 0.3278727109460142 1104 76-77 0.3447800661692568 1104 78-79 0.5430076380605477 1104 80-81 0.7297481269106818 1104 82-83 0.6566471593391583 1104 84-85 0.7958122507840741 1104 86-87 0.607805109342852 1104 88-89 0.7054538097883096 1104 90-91 0.8031680893914235 1104 92-93 0.8257836446329812 1104 94-95 0.7827752676936655 1104 96-97 0.9348921454030652 1104 98-99 0.828304368174738 1104 100-101 0.8852475458740336 1105 1 0.2343575007568326 1105 2 0.05002840822003307 1105 3 0.09835216526039403 1105 4 0.05861233921682896 1105 5 0.10794321939791018 1105 6 0.10648439985669 1105 7 0.19022358773987946 1105 8 0.18565062418439027 1105 9 0.25538387383464567 1105 10-11 0.26404948878268186 1105 12-13 0.31664782311668205 1105 14-15 0.39862023783842204 1105 16-17 0.3337348975716736 1105 18-19 0.3173083418017413 1105 20-21 0.2942745398929887 1105 22-23 0.2957826178554228 1105 24-25 0.2703583514657666 1105 26-27 0.37404994713927664 1105 28-29 0.28191954409775377 1105 30-31 0.44765897237063257 1105 32-33 0.457795072288917 1105 34-35 0.4406635177624665 1105 36-37 0.33748203880296046 1105 38-39 0.39838328599620354 1105 40-41 0.1987636047312762 1105 42-43 0.24696372325687577 1105 44-45 0.36568531712284624 1105 46-47 0.2774321749386459 1105 48-49 0.30328805313290275 1105 50-51 0.24252502038845591 1105 52-53 0.3359397197144105 1105 54-55 0.38326392090873895 1105 56-57 0.36547298133734074 1105 58-59 0.30531227982901754 1105 60-61 0.46566620493027955 1105 62-63 0.3265689276593591 1105 64-65 0.30160570392637 1105 66-67 0.24576230791159048 1105 68-69 0.35365968223377564 1105 70-71 0.23025884988669532 1105 72-73 0.3741588618037781 1105 74-75 0.30374059831990863 1105 76-77 0.25976466733956727 1105 78-79 0.3166011182893804 1105 80-81 0.4880560288434701 1105 82-83 0.6131935284817658 1105 84-85 0.5499904199098893 1105 86-87 0.5386336597063561 1105 88-89 0.5867959344535016 1105 90-91 0.7035757850732196 1105 92-93 0.7510078142627599 1105 94-95 0.7520654749712463 1105 96-97 1.076003660927885 1105 98-99 1.0097626840094023 1105 100-101 1.0332775222624946 1106 1 0.37792897347174303 1106 2 0.24814142142482254 1106 3 0.28421748054787344 1106 4 0.26537232520863085 1106 5 0.23311986690630704 1106 6 0.2506153765165564 1106 7 0.28360285972554067 1106 8 0.2702727118180306 1106 9 0.4263259252117777 1106 10-11 0.4178035830908229 1106 12-13 0.4012291221711308 1106 14-15 0.6186247699027732 1106 16-17 0.6279550734981711 1106 18-19 0.5698618510615674 1106 20-21 0.5001821887816078 1106 22-23 0.5847709374895658 1106 24-25 0.5234466443195274 1106 26-27 0.5380370307558593 1106 28-29 0.5551068213570929 1106 30-31 0.6461463958920532 1106 32-33 0.6811364397363349 1106 34-35 0.5515988740445223 1106 36-37 0.5910604126158674 1106 38-39 0.6990022217850935 1106 40-41 0.6218717768673159 1106 42-43 0.6748861837558096 1106 44-45 0.7851139680096892 1106 46-47 0.663020313290211 1106 48-49 0.7384232940533266 1106 50-51 0.777882229460829 1106 52-53 0.6568990753372574 1106 54-55 0.7906702410784803 1106 56-57 0.828906123087954 1106 58-59 0.8459008301864301 1106 60-61 0.8950317159205383 1106 62-63 1.006404331322095 1106 64-65 0.8268248910388252 1106 66-67 0.9467071403282077 1106 68-69 1.0437617566832316 1106 70-71 0.9879480120727919 1106 72-73 1.0836430304789815 1106 74-75 0.8728554037501404 1106 76-77 0.8722289773327674 1106 78-79 0.6933331908848146 1106 80-81 0.8187813651432485 1106 82-83 0.9917970346515546 1106 84-85 1.0707578838078788 1106 86-87 0.9808286208748065 1106 88-89 1.2034570390411865 1106 90-91 1.5021480817998345 1106 92-93 1.6373120187824632 1106 94-95 1.6187641750927853 1106 96-97 1.8267335292920173 1106 98-99 1.9407276017112345 1106 100-101 1.8591828021174663 1107 1 0.14495552044771998 1107 2 0.04656894471737516 1107 3 0.1550093885266861 1107 4 0.13530256763491622 1107 5 0.1676184973237298 1107 6 0.14721771262023964 1107 7 0.18733082175856453 1107 8 0.13536227322649097 1107 9 0.21116669432588964 1107 10-11 0.20919955469948803 1107 12-13 0.226862403352861 1107 14-15 0.2813961300527481 1107 16-17 0.3503396678913475 1107 18-19 0.40208908661487897 1107 20-21 0.2527340968946632 1107 22-23 0.2329097671640028 1107 24-25 0.2951072131152799 1107 26-27 0.2370474787653123 1107 28-29 0.26272354211557314 1107 30-31 0.4060745848359275 1107 32-33 0.3668238980348022 1107 34-35 0.3746189467826895 1107 36-37 0.2908592699231676 1107 38-39 0.287828603098113 1107 40-41 0.38949916147115005 1107 42-43 0.4018489532176943 1107 44-45 0.40257137922488795 1107 46-47 0.36178188929915933 1107 48-49 0.3627737150591699 1107 50-51 0.5138894982432163 1107 52-53 0.537621112475712 1107 54-55 0.3720297806789148 1107 56-57 0.40607385274815044 1107 58-59 0.37008923793211324 1107 60-61 0.37185153867500986 1107 62-63 0.31541683281395905 1107 64-65 0.40437762243263364 1107 66-67 0.37665171727832103 1107 68-69 0.39180143608827933 1107 70-71 0.4526121733091344 1107 72-73 0.5241339910661651 1107 74-75 0.5652605182847559 1107 76-77 0.46699546030470174 1107 78-79 0.5122015689546267 1107 80-81 0.5996578539442154 1107 82-83 0.5498834764028189 1107 84-85 0.6793665477417399 1107 86-87 0.6109726310302079 1107 88-89 0.6971895653006968 1107 90-91 1.0036444600986982 1107 92-93 1.1640996863935094 1107 94-95 1.0627089819322464 1107 96-97 1.2821061545465788 1107 98-99 1.1239219876287514 1107 100-101 1.0467630719899468 1108 1 0.01219512634244424 1108 2 -0.07619359735854303 1108 3 -0.018745351461788573 1108 4 0.03669748017060215 1108 5 0.012174540653361987 1108 6 0.03497710167378898 1108 7 0.04079336053485605 1108 8 0.05693965389164646 1108 9 0.027110794515991188 1108 10-11 0.012931472155287338 1108 12-13 0.024764377399456805 1108 14-15 0.023305969146129257 1108 16-17 0.028859603071722972 1108 18-19 -0.02268014900445081 1108 20-21 -0.0576258311092559 1108 22-23 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2316 86-87 -0.7398306619225217 2316 88-89 -0.8013400611508494 2316 90-91 -0.81719744500381 2316 92-93 -1.1006468265691005 2316 94-95 -0.8825278237359768 2316 96-97 -0.7362291011782958 2316 98-99 -0.6486980091348364 2316 100-101 -0.6291286814095329 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 30.0 5 70.0 6 174.0 7 373.0 8 965.0 9 1843.0 10 3089.0 11 4962.0 12 7357.0 13 10016.0 14 12444.0 15 17189.0 16 22581.0 17 30444.0 18 27416.0 19 17417.0 20 15507.0 21 16414.0 22 17311.0 23 19070.0 24 22677.0 25 27340.0 26 33851.0 27 41528.0 28 52356.0 29 65297.0 30 80461.0 31 100058.0 32 125225.0 33 160695.0 34 212752.0 35 297396.0 36 443913.0 37 645877.0 38 451337.0 39 32576.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.558757465434155 19.82281130471034 15.225508968462744 22.392922261392762 2 15.452611265355243 21.238141343292437 39.46111639616785 23.84813099518447 3 18.982664682533766 25.66208175568591 30.176779349378037 25.178474212402293 4 13.243241004435033 17.501470337291234 37.04424928305525 32.21103937521848 5 13.868621361951302 37.82228681322905 33.52399167120615 14.785100153613495 6 31.22816643986106 36.65294567301623 17.545277456649313 14.573610430473396 7 27.878684660368275 33.43206255494084 20.869683060312077 17.819569724378805 8 26.65532368904116 34.46604064916733 19.812393556040053 19.066242105751453 9 26.80333463661606 16.542236300620935 20.33704216671858 36.31738689604443 10-11 24.774516789741348 27.087706718828926 27.13368059208385 21.004095899345877 12-13 25.945181972121635 24.45084970908748 27.859361329435174 21.74460698935571 14-15 22.979867595310832 25.31282098859517 26.461090383761537 25.246221032332457 16-17 22.0396982127355 28.772208268997556 26.793675986242636 22.394417532024306 18-19 22.03449612659956 27.027239713454517 29.552388652162676 21.385875507783247 20-21 23.00055948565094 26.058098037061907 29.536336000209413 21.40500647707774 22-23 22.738069992909256 26.043680956388627 29.480768848450307 21.737480202251806 24-25 22.413734544967152 26.303133444900666 29.517959209167543 21.765172800964642 26-27 22.308557129428028 26.445320900223095 29.383262816674694 21.862859153674183 28-29 22.090747886658665 26.481153102452836 29.547132030588713 21.880966980299778 30-31 22.31766028538076 26.128039409487343 29.490438841476397 22.063861463655503 32-33 22.202932348042125 26.35132112720018 29.378715841100917 22.067030683656778 34-35 22.504262020222438 26.301129746001816 29.22384014234899 21.970768091426756 36-37 22.586198012836984 26.17393334251184 29.143163313944154 22.096705330707024 38-39 22.383560504534284 26.428854141625536 29.244671426959645 21.942913926880532 40-41 22.55699576937765 26.229924839560255 29.168165374505755 22.044914016556337 42-43 22.380238082457407 26.362118473955466 28.9334037123262 22.32423973126093 44-45 22.539875298314033 26.503755381249682 28.63392260278114 22.322446717655136 46-47 22.690637401058677 26.699930268701582 28.26880993077069 22.340622399469055 48-49 22.811084644101932 26.933565229838308 27.88034710254727 22.37500302351249 50-51 22.673122126007367 26.972290386989588 27.864499634527874 22.49008785247517 52-53 22.94940655670349 27.035682807705818 27.61454681608228 22.400363819508414 54-55 22.7791737831462 26.940626484354457 27.582273517020656 22.697926215478688 56-57 22.76232820417835 27.31347509458638 27.48468070914976 22.43951599208551 58-59 22.972142142024396 26.975264758912537 27.497534361956827 22.555058737106243 60-61 22.915284328095233 26.81828777393912 27.922153981292475 22.344273916673167 62-63 22.745641181966985 26.928681718477677 27.916879278467338 22.408797821087997 64-65 22.7547117484028 26.9200992904441 27.771751924982468 22.553437036170635 66-67 22.661452867893058 27.262023374951166 27.478788224188268 22.59773553296751 68-69 22.607918436590875 27.00997673971677 27.724832837423214 22.65727198626914 70-71 22.6338766436112 26.994744718500908 27.645007316903865 22.726371320984033 72-73 22.847908793121842 26.982211504371413 27.41501210397022 22.754867598536524 74-75 22.71109484579157 27.28657746107161 27.229851330819205 22.772476362317615 76-77 22.798832998121267 27.173499580846855 27.289967826268473 22.737699594763402 78-79 22.700059559162792 27.304537844919206 27.183746229921564 22.81165636599644 80-81 22.671036475399447 27.332533972689305 27.165277947371763 22.83115160453949 82-83 22.682532158785033 27.293585771422336 27.25188534154508 22.771996728247558 84-85 22.777280375572172 27.192350845057263 27.150086384514804 22.880282394855765 86-87 22.736652223622915 27.07975439591126 27.252636850918332 22.93095652954749 88-89 22.753509473573992 27.094269201789018 27.064398169102848 23.087823155534142 90-91 22.62672336184328 27.156875836436644 27.352037484290147 22.864363317429923 92-93 22.772715683248567 26.999063458668836 27.18850888803071 23.039711970051883 94-95 22.619507556303656 26.950358017369062 27.15758065745309 23.27255376887419 96-97 22.692109010023657 26.96946713360444 27.144353422724816 23.194070433647088 98-99 22.415364173456638 26.917257855549998 27.06971334656536 23.597664624428003 100-101 22.161786396919403 27.247248205798208 27.1278639344026 23.46310146287979 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 771.0 1 564.0 2 401.0 3 465.0 4 418.0 5 421.0 6 757.0 7 1632.5 8 2861.5 9 3510.5 10 3223.0 11 2931.5 12 2975.5 13 3251.0 14 3650.5 15 3989.5 16 4253.0 17 4465.0 18 4780.5 19 5090.0 20 5511.0 21 6299.0 22 7471.5 23 8928.0 24 10678.0 25 13453.0 26 17247.0 27 20958.0 28 23717.5 29 27451.0 30 33260.5 31 39043.5 32 44426.5 33 50033.5 34 57516.0 35 63425.0 36 69197.0 37 76572.0 38 80915.0 39 82591.0 40 84161.0 41 89003.0 42 93348.5 43 98432.0 44 104591.0 45 106199.0 46 105838.0 47 108384.5 48 116378.5 49 119047.5 50 114968.5 51 111772.5 52 108078.5 53 106691.5 54 105424.5 55 98987.0 56 90138.0 57 81708.0 58 75617.0 59 69525.0 60 59665.5 61 47534.5 62 36527.5 63 28110.5 64 21144.5 65 15219.0 66 10553.0 67 7902.5 68 5602.5 69 3355.0 70 1968.0 71 1159.5 72 758.5 73 540.5 74 370.5 75 231.5 76 150.5 77 83.5 78 47.0 79 28.5 80 20.0 81 14.5 82 8.5 83 3.0 84 2.0 85 0.5 86 1.0 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014777900742871155 2 0.0025513416080741678 3 0.0025513416080741678 4 1.6567153299182908E-4 5 0.0018555211695084858 6 5.632832121722188E-4 7 3.3134306598365814E-5 8 0.0 9 0.0 10-11 4.9701459897548725E-5 12-13 5.467160588730359E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0023028343085864243 22-23 0.0 24-25 0.003694475185717789 26-27 0.0018886554761068516 28-29 0.0036282065725210567 30-31 0.01565595986772785 32-33 9.608948913526087E-4 34-35 0.0028164160608610943 36-37 0.0032968635065373985 38-39 0.0022697000019880585 40-41 0.004721638690267128 42-43 0.0024519386882790703 44-45 5.798503654714018E-4 46-47 2.153729928893778E-4 48-49 2.3194014618856072E-4 50-51 1.988058395901949E-4 52-53 0.0013253722639346326 54-55 0.002766714600963546 56-57 3.810445258812069E-4 58-59 0.0012756708040370839 60-61 0.0012094021908403522 62-63 0.0 64-65 1.82238686291012E-4 66-67 9.940291979509745E-5 68-69 0.0 70-71 2.816416060861094E-4 72-73 6.626861319673163E-5 74-75 3.4791021928284107E-4 76-77 9.940291979509744E-4 78-79 4.1417883247957265E-4 80-81 0.006345219713587053 82-83 0.0021702970821929606 84-85 0.003909848178607166 86-87 0.0024353715349798876 88-89 0.0015241781035248275 90-91 2.982087593852924E-4 92-93 1.6567153299182907E-5 94-95 3.3134306598365816E-4 96-97 0.001954924089303583 98-99 0.0029820875938529235 100-101 0.0011431335776436206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3018020.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.939580291258864 #Duplication Level Percentage of deduplicated Percentage of total 1 67.7792303196656 29.10411702391008 2 15.617188559579413 13.411910441555792 3 6.2736263609167455 8.08160648525828 4 3.135791786049706 5.385983326950056 5 1.780891756469153 3.8235372283474107 6 1.1553996441250718 2.976742547244026 7 0.7949581943360141 2.389461984971981 8 0.532210160709916 1.8282304742101776 9 0.4207898856743355 1.6261686973496492 >10 2.2544621618328295 17.63482873401894 >50 0.16421904721039432 4.891801261916559 >100 0.0872975041522081 6.573222183594307 >500 0.0030088265072561 0.8321991313176909 >1k 6.943445785255442E-4 0.41353217449024876 >5k 7.714939761394934E-5 0.22662609995095667 >10k+ 1.5429879522789867E-4 0.8000322049138435 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13857 0.4591420865335551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10293 0.34105141781697934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6841 0.22667179143942054 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.626861319673163E-5 7 0.0 0.0 0.0 0.0 6.626861319673163E-5 8 0.0 0.0 0.0 0.0 6.626861319673163E-5 9 0.0 0.0 0.0 0.0 6.626861319673163E-5 10-11 0.0 0.0 0.0 0.0 8.283576649591454E-5 12-13 0.0 0.0 0.0 0.0 9.940291979509745E-5 14-15 0.0 0.0 0.0 0.0 9.940291979509745E-5 16-17 0.0 0.0 0.0 3.3134306598365814E-5 1.1597007309428035E-4 18-19 0.0 0.0 0.0 6.626861319673163E-5 1.3253722639346326E-4 20-21 0.0 0.0 0.0 8.283576649591454E-5 1.3253722639346326E-4 22-23 0.0 0.0 0.0 1.6567153299182908E-4 1.6567153299182908E-4 24-25 3.3134306598365814E-5 0.0 0.0 3.1477591268447524E-4 1.988058395901949E-4 26-27 3.3134306598365814E-5 0.0 0.0 7.620890517624138E-4 1.988058395901949E-4 28-29 6.626861319673163E-5 0.0 0.0 0.0017726854030125712 2.153729928893778E-4 30-31 6.626861319673163E-5 0.0 0.0 0.004572534310574482 2.3194014618856072E-4 32-33 6.626861319673163E-5 0.0 0.0 0.008018502196804528 2.650744527869265E-4 34-35 6.626861319673163E-5 0.0 0.0 0.011315365703341925 2.650744527869265E-4 36-37 6.626861319673163E-5 0.0 0.0 0.0129886481865594 2.650744527869265E-4 38-39 6.626861319673163E-5 0.0 0.0 0.015407452568240103 2.650744527869265E-4 40-41 6.626861319673163E-5 0.0 0.0 0.017958794176314272 2.650744527869265E-4 42-43 6.626861319673163E-5 0.0 0.0 0.020758643083876184 2.816416060861094E-4 44-45 6.626861319673163E-5 0.0 0.0 0.024121775203610314 3.1477591268447524E-4 46-47 9.940291979509745E-5 0.0 0.0 0.02837953360150032 3.3134306598365816E-4 48-49 9.940291979509745E-5 0.0 0.0 0.0339295299567266 3.3134306598365816E-4 50-51 9.940291979509745E-5 0.0 0.0 0.0421634051464205 3.4791021928284107E-4 52-53 9.940291979509745E-5 0.0 0.0 0.059409811730869906 3.810445258812069E-4 54-55 9.940291979509745E-5 0.0 0.0 0.08069860372031995 4.141788324795727E-4 56-57 9.940291979509745E-5 0.0 0.0 0.10637769133405345 4.307459857787556E-4 58-59 9.940291979509745E-5 0.0 0.0 0.1448134869881578 4.4731313907793853E-4 60-61 9.940291979509745E-5 0.0 0.0 0.19519420017097303 4.6388029237712144E-4 62-63 9.940291979509745E-5 0.0 0.0 0.23245372794083538 4.970145989754872E-4 64-65 9.940291979509745E-5 0.0 0.0 0.2685038535198574 4.970145989754872E-4 66-67 9.940291979509745E-5 1.6567153299182907E-5 0.0 0.3354848543084539 5.135817522746701E-4 68-69 1.3253722639346326E-4 3.3134306598365814E-5 0.0 0.42322449818092656 5.30148905573853E-4 70-71 1.4910437969264617E-4 3.3134306598365814E-5 0.0 0.49312131795017927 5.30148905573853E-4 72-73 1.6567153299182908E-4 3.3134306598365814E-5 0.0 0.5559936647205783 5.964175187705847E-4 74-75 1.6567153299182908E-4 3.3134306598365814E-5 0.0 0.6778616443893679 5.964175187705847E-4 76-77 1.6567153299182908E-4 6.626861319673163E-5 0.0 0.7641765130781109 5.964175187705847E-4 78-79 1.6567153299182908E-4 9.940291979509745E-5 0.0 0.8313563197062975 5.964175187705847E-4 80-81 2.650744527869265E-4 1.1597007309428035E-4 0.0 0.9167765621168846 6.958204385656821E-4 82-83 2.650744527869265E-4 1.4910437969264617E-4 0.0 1.0833427213868696 7.620890517624138E-4 84-85 2.650744527869265E-4 1.6567153299182908E-4 0.0 1.254928728106507 7.620890517624138E-4 86-87 2.650744527869265E-4 1.6567153299182908E-4 0.0 1.4672864990954335 7.620890517624138E-4 88-89 2.650744527869265E-4 2.3194014618856072E-4 0.0 1.7412243788974229 7.620890517624138E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7965 0.0 44.495564 1 GTATCAA 14505 0.0 42.021603 1 TATCAAC 18765 0.0 32.401142 2 ATCAACG 18755 0.0 32.214203 3 TCAACGC 18875 0.0 32.00887 4 CAACGCA 19165 0.0 31.525038 5 AACGCAG 19740 0.0 30.654373 6 ACGCAGA 22580 0.0 26.588465 7 CGCAGAG 22815 0.0 26.127232 8 GCAGAGT 24370 0.0 23.914383 9 GTACATG 14955 0.0 23.634706 1 GAGTACT 12370 0.0 23.114777 12-13 TACATGG 15455 0.0 22.374908 2 AGTACTT 13015 0.0 21.969252 12-13 ACATGGG 15445 0.0 21.68096 3 AGAGTAC 20875 0.0 21.353937 10-11 GTACTTT 13495 0.0 21.241507 14-15 CAGAGTA 23645 0.0 19.223955 10-11 TACTTTT 15625 0.0 18.376263 14-15 CATGGGA 8930 0.0 18.296885 4 >>END_MODULE