FastQCFastQC Report
Thu 26 May 2016
SRR937138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728948
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121730.4460693278142347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82310.301618059413371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53590.19637603941152415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.047.0357971
GTATCAA122250.042.3358271
ATCAACG155900.033.045493
TATCAAC156050.033.044792
TCAACGC158300.032.5745054
CAACGCA160200.032.193485
AACGCAG164450.031.3314296
ACGCAGA189100.026.9712437
CGCAGAG189700.026.6112868
GTACATG140650.024.9370821
GCAGAGT202000.024.308479
TACATGG145300.023.7469582
GAGTACT92000.023.45292912-13
ACATGGG145950.022.6638743
CATGGGG67500.020.9113334
GTACTTT103150.020.7081714-15
AGAGTAC173250.020.61658110-11
CAGAGTA194000.020.0161810-11
AGTACTT98150.019.99810212-13
CATGGGA86300.017.9529514
Produced by FastQC (version 0.11.5)