##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937138_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2728948 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.594990450532585 31.0 31.0 34.0 30.0 34.0 2 31.702286009114136 33.0 31.0 34.0 30.0 34.0 3 31.707262652128218 33.0 31.0 34.0 30.0 34.0 4 35.31332476837228 37.0 35.0 37.0 33.0 37.0 5 35.3182658665537 37.0 35.0 37.0 33.0 37.0 6 35.376079720097266 37.0 35.0 37.0 33.0 37.0 7 35.30546129863962 37.0 35.0 37.0 33.0 37.0 8 35.25626944888653 37.0 35.0 37.0 32.0 37.0 9 36.85934909716125 39.0 37.0 39.0 33.0 39.0 10-11 36.6702806356149 39.0 37.0 39.0 32.0 39.0 12-13 36.589340471126604 39.0 37.0 39.0 32.0 39.0 14-15 37.701119992026236 40.0 37.0 41.0 32.0 41.0 16-17 37.634178994982676 40.0 37.0 41.0 32.0 41.0 18-19 37.5970199871892 40.0 37.0 41.0 32.0 41.0 20-21 37.52509996526134 40.0 37.0 41.0 32.0 41.0 22-23 37.406759674423995 40.0 37.0 41.0 32.0 41.0 24-25 37.24263873844426 39.5 37.0 41.0 31.0 41.0 26-27 37.338255437626515 40.0 37.0 41.0 31.0 41.0 28-29 37.29017775347863 40.0 37.0 41.0 31.0 41.0 30-31 37.133925050972024 39.5 36.5 41.0 31.0 41.0 32-33 36.93432285994456 39.0 36.0 41.0 30.5 41.0 34-35 36.75788234147371 39.0 36.0 41.0 30.0 41.0 36-37 36.57824022297237 39.0 35.5 41.0 30.0 41.0 38-39 36.41741213097501 39.0 35.0 41.0 30.0 41.0 40-41 36.24136572041681 39.0 35.0 41.0 30.0 41.0 42-43 36.01441874304677 39.0 35.0 40.0 29.0 41.0 44-45 35.74491690570872 39.0 35.0 40.0 27.5 41.0 46-47 35.37593882331213 38.0 35.0 40.0 26.5 41.0 48-49 35.05397464517463 38.0 34.0 40.0 25.5 41.0 50-51 34.384908030493804 38.0 33.5 39.5 24.5 40.5 52-53 34.61629059989417 38.0 34.0 39.5 25.5 40.5 54-55 34.98844976159311 38.5 34.5 40.0 26.0 41.0 56-57 34.87167692458779 38.0 34.0 40.0 25.5 41.0 58-59 34.61707002112169 38.0 34.0 40.0 25.0 41.0 60-61 34.32594080209663 38.0 34.0 40.0 24.0 41.0 62-63 33.99215686777469 37.0 34.0 40.0 23.0 41.0 64-65 33.66347709813452 37.0 33.0 40.0 22.5 41.0 66-67 33.27983823803165 36.0 33.0 39.0 22.0 41.0 68-69 32.88945392143786 36.0 32.5 39.0 21.0 41.0 70-71 32.476846755599595 35.0 32.0 39.0 20.0 40.0 72-73 32.08769551490171 35.0 32.0 38.0 18.5 40.0 74-75 31.657634370460705 35.0 31.0 37.0 18.0 39.0 76-77 31.234608537795516 35.0 31.0 36.5 15.0 39.0 78-79 30.803668483239697 35.0 31.0 36.0 11.0 39.0 80-81 30.374128968379026 34.5 30.0 36.0 7.5 37.5 82-83 29.98013153786734 34.0 30.0 35.0 7.0 37.0 84-85 29.635348310044748 34.0 30.0 35.0 2.0 36.5 86-87 29.312647950785433 34.0 29.0 35.0 2.0 36.0 88-89 28.971605908210783 34.0 29.0 35.0 2.0 36.0 90-91 28.620525015500476 34.0 29.0 35.0 2.0 35.5 92-93 28.24594843873903 34.0 29.0 35.0 2.0 35.0 94-95 27.821474062532523 34.0 27.5 35.0 2.0 35.0 96-97 27.340299998387657 34.0 27.0 35.0 2.0 35.0 98-99 26.71629268861114 33.5 25.5 35.0 2.0 35.0 100-101 24.460137752716435 31.0 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31670245560843924 1101 2 0.4479960681203714 1101 3 0.3479454900415071 1101 4 0.2580177806284567 1101 5 0.27152914467023237 1101 6 0.16578872768653952 1101 7 0.22638197040186014 1101 8 0.2255430335447457 1101 9 0.12733484479226576 1101 10-11 0.23312908860055614 1101 12-13 0.36800497264919585 1101 14-15 0.6548704686451146 1101 16-17 0.662372283368434 1101 18-19 0.4829593861430297 1101 20-21 0.5297282849853104 1101 22-23 0.5062254888414515 1101 24-25 0.5163089211833451 1101 26-27 0.5934830568562148 1101 28-29 0.6433452192830131 1101 30-31 0.6190425949980138 1101 32-33 0.697127009929865 1101 34-35 0.5858144501777005 1101 36-37 0.36684194770453615 1101 38-39 0.5933412814671684 1101 40-41 0.7203069095569603 1101 42-43 0.6053649861220762 1101 44-45 0.6417776873243355 1101 46-47 0.5245371657030375 1101 48-49 0.6499208882608585 1101 50-51 0.6419319290970904 1101 52-53 0.7514913844123399 1101 54-55 0.6289039633141371 1101 56-57 0.6946909538613681 1101 58-59 0.6564814708782194 1101 60-61 0.7528651116822047 1101 62-63 0.7509766254943386 1101 64-65 0.9088426762797681 1101 66-67 0.8767623282394865 1101 68-69 0.7294290689770051 1101 70-71 0.7369131917266785 1101 72-73 0.8495688989544234 1101 74-75 0.7677259517738406 1101 76-77 0.7859758620536432 1101 78-79 0.9296983807442345 1101 80-81 0.8488378508708756 1101 82-83 0.8928899323981128 1101 84-85 0.8874563614871747 1101 86-87 0.8152817748869268 1101 88-89 0.8410563431905906 1101 90-91 0.9841959784211021 1101 92-93 1.050537520518084 1101 94-95 1.0872061829127269 1101 96-97 0.9280804727026712 1101 98-99 0.9317059242752812 1101 100-101 1.0087599721659188 1102 1 0.40771080013163896 1102 2 0.26339639389700054 1102 3 0.25264414160224646 1102 4 0.21654504608461167 1102 5 0.20869700845793204 1102 6 0.15522167584159519 1102 7 0.2326519716674511 1102 8 0.2358564607680833 1102 9 0.20877009914072886 1102 10-11 0.44975086934081077 1102 12-13 0.4588610614661661 1102 14-15 0.3948542435486502 1102 16-17 0.5304736203151634 1102 18-19 0.30523878659344206 1102 20-21 0.3846014843086607 1102 22-23 0.3149375401183363 1102 24-25 0.46923585756483277 1102 26-27 0.4824998259253732 1102 28-29 0.378878271568837 1102 30-31 0.4563047156903508 1102 32-33 0.35084320751911946 1102 34-35 0.2836290597854685 1102 36-37 0.2404965712652043 1102 38-39 0.3441785613931856 1102 40-41 0.1663393939441704 1102 42-43 0.20581647234516254 1102 44-45 0.3041826779932322 1102 46-47 0.2509992175616347 1102 48-49 0.4073181128770713 1102 50-51 0.24873187127027307 1102 52-53 0.21395200658479752 1102 54-55 0.3004978134785432 1102 56-57 0.40014869875506065 1102 58-59 0.3212005965318667 1102 60-61 0.21886160706947067 1102 62-63 0.2237158230025642 1102 64-65 0.39851361881947156 1102 66-67 0.10587125197172043 1102 68-69 -0.05101612873132666 1102 70-71 0.1989244825242551 1102 72-73 0.3020327958891542 1102 74-75 0.18005306946609068 1102 76-77 0.2385192253386954 1102 78-79 0.16005494135156795 1102 80-81 0.24834488634909135 1102 82-83 0.0582340146133582 1102 84-85 0.17989253068504496 1102 86-87 0.15268254438018403 1102 88-89 0.14446731614299324 1102 90-91 0.2208945709005583 1102 92-93 0.36181869715018067 1102 94-95 0.3096176259862382 1102 96-97 0.4047009767206511 1102 98-99 0.6245572001932729 1102 100-101 0.4193706470411058 1103 1 0.21521798265942493 1103 2 0.24455836294634636 1103 3 0.3032430968319204 1103 4 0.2029508602211365 1103 5 0.1846440985515727 1103 6 0.12799517025133156 1103 7 0.08546842567849922 1103 8 0.30103737620316906 1103 9 0.155925829841145 1103 10-11 0.4082498639137029 1103 12-13 0.26959075050255166 1103 14-15 0.35169631688387426 1103 16-17 0.4215327800788842 1103 18-19 0.35952404778699787 1103 20-21 0.5408033963854351 1103 22-23 0.46616371449991334 1103 24-25 0.4993674613307846 1103 26-27 0.5194125766213915 1103 28-29 0.5119413161101605 1103 30-31 0.5605632344150564 1103 32-33 0.6224406769910615 1103 34-35 0.508529760570795 1103 36-37 0.3713227867650275 1103 38-39 0.3552481099526119 1103 40-41 0.395453057629247 1103 42-43 0.4937583497054874 1103 44-45 0.5487243177337504 1103 46-47 0.4127974447563645 1103 48-49 0.4779097943892836 1103 50-51 0.4673471165841434 1103 52-53 0.5922032597844762 1103 54-55 0.5768908357032743 1103 56-57 0.6715324332871901 1103 58-59 0.5245450826385607 1103 60-61 0.5172436779508587 1103 62-63 0.6469363613746921 1103 64-65 0.48678010215751755 1103 66-67 0.5316464178345228 1103 68-69 0.5099433875401331 1103 70-71 0.35633360933291414 1103 72-73 0.31786083615772753 1103 74-75 0.1621833558094643 1103 76-77 0.2633510511002939 1103 78-79 0.3176464475065295 1103 80-81 0.4356553995723722 1103 82-83 0.5102489357796429 1103 84-85 0.4298342083058522 1103 86-87 0.39653406847659056 1103 88-89 0.377365790144772 1103 90-91 0.469894020251477 1103 92-93 0.4574673990286122 1103 94-95 0.4206037481858651 1103 96-97 0.5081961842400631 1103 98-99 0.576788928464822 1103 100-101 0.7216760682720498 1104 1 0.2696413816649148 1104 2 0.2738760119851449 1104 3 0.32382271600505774 1104 4 0.22497645702923563 1104 5 0.1859754138273857 1104 6 0.20549053116389615 1104 7 0.21440492187351623 1104 8 0.1733591335658673 1104 9 0.24098322072095613 1104 10-11 0.423604717381842 1104 12-13 0.4369992636804838 1104 14-15 0.48075965239105756 1104 16-17 0.4966759516700634 1104 18-19 0.4760930240189154 1104 20-21 0.5558676139399239 1104 22-23 0.5269663680711858 1104 24-25 0.5582743481037014 1104 26-27 0.34913115539187345 1104 28-29 0.382507649611604 1104 30-31 0.530204479583837 1104 32-33 0.5185474824200327 1104 34-35 0.44761054939475997 1104 36-37 0.5735924476165124 1104 38-39 0.6734953830258519 1104 40-41 0.41647725404036606 1104 42-43 0.3639526938756532 1104 44-45 0.40663917609920475 1104 46-47 0.5099713776999266 1104 48-49 0.6953421781856193 1104 50-51 0.5847278738901736 1104 52-53 0.4815390159997932 1104 54-55 0.6097500828245259 1104 56-57 0.5234676496228516 1104 58-59 0.5573487516824329 1104 60-61 0.669270235648483 1104 62-63 0.5978276100970561 1104 64-65 0.6016102379272255 1104 66-67 0.4892629929703034 1104 68-69 0.4247204943377838 1104 70-71 0.39119148770779333 1104 72-73 0.4900176976764996 1104 74-75 0.5084583263810387 1104 76-77 0.4244322868231727 1104 78-79 0.4027055914885267 1104 80-81 0.549946764397486 1104 82-83 0.4980155992054378 1104 84-85 0.5228344313762605 1104 86-87 0.4435774758552782 1104 88-89 0.503826384861469 1104 90-91 0.5178013279584341 1104 92-93 0.608701933587561 1104 94-95 0.6283884316068793 1104 96-97 0.6301444360749962 1104 98-99 0.7885510690765045 1104 100-101 0.8906248475538305 1105 1 0.20139781778031818 1105 2 0.29461468318802986 1105 3 0.24618600739865926 1105 4 0.28065562003052236 1105 5 0.2538624674799692 1105 6 0.2577537440850364 1105 7 0.2305858337757556 1105 8 0.21591423971813128 1105 9 0.28275704867681384 1105 10-11 0.3068488609306286 1105 12-13 0.2834738379623616 1105 14-15 0.37192518715688294 1105 16-17 0.2537488360896205 1105 18-19 0.10916688223601767 1105 20-21 0.1895335337923214 1105 22-23 0.22898551412579593 1105 24-25 0.3185556331450172 1105 26-27 0.22907791436061586 1105 28-29 0.3184423588408478 1105 30-31 -0.06808014520397876 1105 32-33 0.07531963073786585 1105 34-35 0.13063997307261133 1105 36-37 -0.1220390479372071 1105 38-39 -0.09376532695221584 1105 40-41 -0.07441828453659838 1105 42-43 0.13754002447465297 1105 44-45 0.14858574494191146 1105 46-47 0.09407816304505445 1105 48-49 0.3013806611867196 1105 50-51 0.15067446456004063 1105 52-53 0.14741827754877335 1105 54-55 0.19756379914405642 1105 56-57 0.2787903406853829 1105 58-59 0.15855529565558157 1105 60-61 0.2266713028624352 1105 62-63 0.27476031630170183 1105 64-65 0.3367280658034346 1105 66-67 0.2971390162499219 1105 68-69 0.4239037948777309 1105 70-71 0.26370160023923717 1105 72-73 0.3232672645079404 1105 74-75 0.30869350442506516 1105 76-77 0.3433446376721285 1105 78-79 0.24206135337453816 1105 80-81 0.41064474709908083 1105 82-83 0.37115606663017786 1105 84-85 0.29146077466785414 1105 86-87 0.33077353149652566 1105 88-89 0.40541146726329913 1105 90-91 0.3151564222014933 1105 92-93 0.1665207323797553 1105 94-95 0.2799757398043283 1105 96-97 0.30032115850334407 1105 98-99 0.3697829880770733 1105 100-101 0.2974918626317091 1106 1 0.26530613327566854 1106 2 0.22444508035449573 1106 3 0.24231981444032513 1106 4 0.10539443073315624 1106 5 0.14422151474109057 1106 6 0.1962818596238094 1106 7 0.22467284422332767 1106 8 0.2864624863047922 1106 9 0.2421114460020135 1106 10-11 0.35629722571093936 1106 12-13 0.3047399903815986 1106 14-15 0.3795144023557029 1106 16-17 0.46557005904767124 1106 18-19 0.3001727654631807 1106 20-21 0.425825667028235 1106 22-23 0.1570342528617914 1106 24-25 0.3709120072376564 1106 26-27 0.19860190673832534 1106 28-29 0.24595790336810097 1106 30-31 0.3447790751847748 1106 32-33 0.29033515581258484 1106 34-35 0.31484150258717847 1106 36-37 0.37669279214110674 1106 38-39 0.4370209001025387 1106 40-41 0.28475731473790233 1106 42-43 0.3150016854572826 1106 44-45 0.3146450545789392 1106 46-47 0.21961203809281216 1106 48-49 0.29367818355519404 1106 50-51 0.25755500415802857 1106 52-53 0.3796742998616267 1106 54-55 0.4136240716819657 1106 56-57 0.3573152484997806 1106 58-59 0.4955579135788497 1106 60-61 0.5102053961677271 1106 62-63 0.41766716040668683 1106 64-65 0.3484779854644273 1106 66-67 0.5959872214415682 1106 68-69 0.45148968952742763 1106 70-71 0.33664615698969413 1106 72-73 0.5206943836096443 1106 74-75 0.6652342567249434 1106 76-77 0.6828503602212841 1106 78-79 0.6123747980386653 1106 80-81 0.5758242182077744 1106 82-83 0.5729708527416832 1106 84-85 0.6364639318156122 1106 86-87 0.5803695021745412 1106 88-89 0.5247776703369524 1106 90-91 0.4941885842582927 1106 92-93 0.6393887628253161 1106 94-95 0.5709493609886138 1106 96-97 0.6715063688903804 1106 98-99 0.646228623276528 1106 100-101 0.46543476202548817 1107 1 0.0666877122230396 1107 2 0.11314260541715271 1107 3 0.10159378060573587 1107 4 0.1000294557958128 1107 5 0.1287390586007433 1107 6 -0.013534869699498131 1107 7 0.07994618382232233 1107 8 0.21233654645516964 1107 9 0.16724459136542436 1107 10-11 0.281516132352543 1107 12-13 0.27684681995553007 1107 14-15 0.21505794872162198 1107 16-17 0.3301044418797474 1107 18-19 0.27929087323260404 1107 20-21 0.3108765755495426 1107 22-23 0.2225950298041539 1107 24-25 0.424208138190032 1107 26-27 0.27819033092379186 1107 28-29 0.2875611301851464 1107 30-31 0.34793579510959205 1107 32-33 0.45052351734140217 1107 34-35 0.40727140546938045 1107 36-37 0.28691561482281003 1107 38-39 0.0032622848143191163 1107 40-41 0.3264842884221153 1107 42-43 0.1961780639033961 1107 44-45 0.2414875514461059 1107 46-47 0.16512112330584472 1107 48-49 0.25562837152511975 1107 50-51 0.19814123536103523 1107 52-53 0.2781196319418271 1107 54-55 0.3085492753160537 1107 56-57 0.2815778581488999 1107 58-59 0.3737111090675711 1107 60-61 0.4568798949146 1107 62-63 0.3660408289530537 1107 64-65 0.3354955293240778 1107 66-67 0.3943091982586182 1107 68-69 0.3322188561940891 1107 70-71 0.1480347848092478 1107 72-73 0.23557259476252312 1107 74-75 0.34877044093546417 1107 76-77 0.19553480571000748 1107 78-79 0.3351955217228806 1107 80-81 0.3501847288594 1107 82-83 0.36202943670158305 1107 84-85 0.3287371930938079 1107 86-87 0.2143987158336884 1107 88-89 0.2743269341214152 1107 90-91 0.3127952571906256 1107 92-93 0.25485359678521746 1107 94-95 0.13281026324425227 1107 96-97 0.0666712341786031 1107 98-99 0.2617948826750265 1107 100-101 0.33415186728864654 1108 1 0.10511288065422164 1108 2 0.10003033801259775 1108 3 0.13633842690025233 1108 4 0.12133900825009647 1108 5 0.11541936017276555 1108 6 -0.0811076560325148 1108 7 0.050683371021555956 1108 8 0.0023441659611052046 1108 9 0.00963811879564247 1108 10-11 0.0276543963082716 1108 12-13 0.2196766643572019 1108 14-15 0.24603464907451666 1108 16-17 0.3194659573593768 1108 18-19 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2316 60-61 -1.9671403820881963 2316 62-63 -1.7794726350764378 2316 64-65 -2.114034163780744 2316 66-67 -1.8793770935907261 2316 68-69 -1.9100172189354439 2316 70-71 -1.6184896023968136 2316 72-73 -2.060703498800649 2316 74-75 -2.29930262104142 2316 76-77 -2.620488821731204 2316 78-79 -2.843432978177482 2316 80-81 -2.934704482479777 2316 82-83 -2.657172685657919 2316 84-85 -3.099757662924528 2316 86-87 -3.326752327908835 2316 88-89 -3.409901032698034 2316 90-91 -2.902121915985937 2316 92-93 -2.850046325992782 2316 94-95 -3.527978895783246 2316 96-97 -3.610893470384969 2316 98-99 -3.4604680956031757 2316 100-101 -3.610123491420005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6165.0 3 2372.0 4 2470.0 5 2614.0 6 3031.0 7 3585.0 8 4297.0 9 5502.0 10 6684.0 11 8191.0 12 10193.0 13 12587.0 14 13821.0 15 16575.0 16 21032.0 17 26915.0 18 23792.0 19 16309.0 20 16006.0 21 17279.0 22 18639.0 23 21012.0 24 23943.0 25 27250.0 26 31815.0 27 37666.0 28 44917.0 29 54282.0 30 65537.0 31 80752.0 32 101896.0 33 131807.0 34 177597.0 35 250466.0 36 373960.0 37 536676.0 38 456628.0 39 74655.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.814568099193615 18.946439981567814 14.614252977725576 22.624738941513 2 15.1144030749435 20.49119179721796 40.42132004816972 23.973085079668824 3 18.876649455383287 25.540044182184253 29.858601615908224 25.724704746524235 4 12.673941885987022 16.87065362958705 37.624898861937886 32.83050562248804 5 13.86903227533385 37.53077800564757 33.61997747865844 14.980212240360135 6 31.00780367104751 36.232398471973596 18.056063813949805 14.703734043029096 7 27.878560535474172 33.25218643765128 21.032409989465204 17.83684303740934 8 26.45563915674557 34.15531731314682 20.118140105555312 19.270903424552298 9 26.867675266496455 16.616119336434743 20.441655693660813 36.074549703407996 10-11 24.663309432983215 26.838067106169078 27.384997327597983 21.113626133249724 12-13 25.863642972748142 24.35622709094753 27.880308835709034 21.8998211005953 14-15 23.02632990216561 25.168268882591217 26.48227967460774 25.32312154063544 16-17 22.094521389222436 28.643519070185246 26.748966516036187 22.51299302455613 18-19 22.090576915991274 27.01551959944501 29.363559489964768 21.530343994598947 20-21 23.10414376653118 26.04045138596081 29.257701961779293 21.59770288572872 22-23 22.748028029671836 26.06413453421399 29.219103782143602 21.96873365397057 24-25 22.445010491208457 26.31825470271855 29.209246923647825 22.027487882425163 26-27 22.255072164200357 26.52819115074605 29.087463062139896 22.129273622913693 28-29 22.04832427091381 26.474518450538838 29.327348080472525 22.14980919807483 30-31 22.28476340631576 26.251281791921283 29.210324554478813 22.25363024728414 32-33 22.160036134058824 26.39197208222514 29.11441322008152 22.333578563634514 34-35 22.48176945330715 26.4164268055186 28.924230996730266 22.177572744443978 36-37 22.52814524820699 26.217489723470944 28.910257326413053 22.344107701909014 38-39 22.308424973128318 26.437062361724905 28.99057759548563 22.263935069661148 40-41 22.49397292106776 26.29120214136521 28.980423651839054 22.234401285727976 42-43 22.3082059152481 26.360134007033096 28.749397173568568 22.58226290415023 44-45 22.42176047116222 26.542121281690523 28.49181807471587 22.544300172431388 46-47 22.633766571663884 26.70695810624737 28.144166032245977 22.515109289842762 48-49 22.66292502099729 26.843821960596927 27.82494125889911 22.668311759506675 50-51 22.512156057818437 26.93573894741008 27.80902185900787 22.743083135763612 52-53 22.83342913248115 27.027196391673336 27.53208874451934 22.607285731326172 54-55 22.608682905466875 26.910337232856406 27.552663717211434 22.92831614446528 56-57 22.610592961055758 27.30242221533568 27.47571379693107 22.611271026677485 58-59 22.808742218170657 26.89526654053881 27.51640196612871 22.779589275161822 60-61 22.78632652178598 26.72963627847175 27.899110323023773 22.584926876718495 62-63 22.607914545745743 26.856640343974924 27.925936323445494 22.60950878683384 64-65 22.53130725064601 26.93828996500507 27.797239765293195 22.733163019055727 66-67 22.497708909770903 27.247618613053415 27.39819560495502 22.85647687222066 68-69 22.420902042385705 26.994616658193532 27.74680736345273 22.83767393596803 70-71 22.458660814160954 26.993747993332367 27.653144475671493 22.89444671683519 72-73 22.66525438482359 26.945549571275944 27.441656328054993 22.947539715845473 74-75 22.482854166265795 27.26345621524447 27.332800060107772 22.920889558381965 76-77 22.647006483894362 27.058671739079838 27.38663264694601 22.9076891300798 78-79 22.58124942149624 27.262378147633243 27.249749582331738 22.90662284853878 80-81 22.48755675452373 27.334763762072793 27.16402137542193 23.013658107981545 82-83 22.500492558472416 27.257883455625294 27.35042030129 22.89120368461229 84-85 22.640087950641202 27.174002881230162 27.140374479286645 23.045534688841986 86-87 22.61243651203527 27.019931656399486 27.343891172181085 23.02374065938416 88-89 22.653515591856095 26.996580957562788 27.158104461028472 23.19179898955265 90-91 22.491482831245985 27.027375704129014 27.46304279026315 23.018098674361852 92-93 22.70283991459179 26.917240178514152 27.30286007532784 23.077059831566217 94-95 22.533270999366092 26.884327301709536 27.305576391578022 23.27682530734635 96-97 22.56491298684969 26.81923340155378 27.324086731250645 23.291766880345875 98-99 22.370454236723877 26.80733808051386 27.267640924550772 23.55456675821149 100-101 22.423023042072835 26.835263085619914 27.31695404400946 23.424759828297788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 459.0 1 344.0 2 293.5 3 395.5 4 390.5 5 389.0 6 644.0 7 1328.0 8 2396.0 9 2957.5 10 2727.5 11 2601.0 12 2762.5 13 2965.0 14 3203.0 15 3477.0 16 3641.5 17 3770.0 18 3977.5 19 4250.5 20 4675.0 21 5324.5 22 6325.5 23 7652.0 24 9229.5 25 11647.5 26 15094.5 27 18561.5 28 21241.5 29 24593.0 30 29766.5 31 35364.0 32 40182.0 33 45211.5 34 52018.0 35 57501.0 36 62962.0 37 69740.5 38 73803.5 39 75067.0 40 76544.0 41 80782.0 42 84604.0 43 89050.5 44 94494.5 45 96166.5 46 95807.0 47 97950.0 48 104940.0 49 107609.5 50 103767.5 51 100856.5 52 98023.5 53 96594.5 54 95287.0 55 89596.5 56 81767.0 57 74296.0 58 68559.0 59 62721.0 60 54047.0 61 43520.0 62 33436.5 63 25589.5 64 19252.0 65 13991.5 66 9791.5 67 7247.0 68 5187.5 69 3147.0 70 1922.5 71 1207.5 72 785.0 73 540.0 74 372.5 75 266.5 76 176.5 77 93.5 78 62.5 79 40.5 80 26.5 81 24.5 82 21.5 83 13.0 84 9.5 85 9.5 86 8.5 87 5.5 88 4.5 89 4.5 90 4.5 91 4.5 92 3.5 93 3.5 94 3.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04071165885168937 2 0.04005206401880872 3 0.025614266010198804 4 0.045475399311382995 5 0.06438378452062846 6 0.06486015856659783 7 0.0657762625011543 8 0.06768175868503173 9 0.06599612744544783 10-11 0.07330663684320844 12-13 0.0731600602136794 14-15 0.0674069275046648 16-17 0.055809051693179935 18-19 0.04882833971185967 20-21 0.055918984165326716 22-23 0.03074444804371502 24-25 0.017332686441808347 26-27 0.013888135647876032 28-29 0.017002889025368017 30-31 0.012678878454261494 32-33 0.008080036702788033 34-35 0.010480229011325977 36-37 0.005753132709014609 38-39 0.008757953614359819 40-41 0.008116680860170294 42-43 0.004983605403987177 44-45 0.001337511744452441 46-47 0.002949854669271822 48-49 8.794597771742078E-4 50-51 0.006027963889381549 52-53 0.005441657371265411 54-55 0.007163932768231567 56-57 0.021931528193281806 58-59 0.008629699063521913 60-61 0.019861133301184195 62-63 0.013814847333111516 64-65 0.009783990021063062 66-67 0.016251683799031715 68-69 0.026273860843079457 70-71 0.01993442161594871 72-73 0.018432011163276106 74-75 0.01889006313055434 76-77 0.026621980338210914 78-79 0.03684570024786108 80-81 0.03156894158481583 82-83 0.030982635066699692 84-85 0.02196817235066407 86-87 0.024734806233024594 88-89 0.020245896953697908 90-91 0.017900670881233354 92-93 0.031990349394711806 94-95 0.02321407370166086 96-97 0.03763354963157964 98-99 0.055131134781608145 100-101 0.06539149884864057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2728948.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.64705645145427 #Duplication Level Percentage of deduplicated Percentage of total 1 71.06633020611993 33.15035116922566 2 14.38704022205215 13.422261548148196 3 5.538481210443899 7.7506153703668605 4 2.8199722736398094 5.261736233600481 5 1.5717011145939306 3.6657615308638327 6 0.9995418299727977 2.7975410500998596 7 0.6795719213925373 2.2190020846014655 8 0.4756364588532307 1.7749632597197165 9 0.3418819197491337 1.4353006689242458 >10 1.9027986810545645 16.22997737274246 >50 0.1433829860105273 4.642543449600594 >100 0.07075010186577038 5.7728826717294695 >500 0.0022816527919709965 0.6841767416557559 >1k 3.9338841240511965E-4 0.24736354676131322 >5k 1.5735536496204786E-4 0.4987641840484046 >10k+ 7.867768248102393E-5 0.4467591179117902 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12173 0.4460693278142347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8231 0.301618059413371 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5359 0.19637603941152415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.664415738225866E-5 0.0 0.0 0.0 4 0.0 3.664415738225866E-5 0.0 0.0 0.0 5 0.0 3.664415738225866E-5 0.0 0.0 0.0 6 0.0 3.664415738225866E-5 0.0 0.0 3.664415738225866E-5 7 0.0 3.664415738225866E-5 0.0 0.0 3.664415738225866E-5 8 0.0 3.664415738225866E-5 0.0 0.0 7.328831476451731E-5 9 0.0 3.664415738225866E-5 0.0 0.0 1.0993247214677597E-4 10-11 0.0 3.664415738225866E-5 0.0 1.832207869112933E-5 1.0993247214677597E-4 12-13 0.0 3.664415738225866E-5 0.0 3.664415738225866E-5 1.0993247214677597E-4 14-15 0.0 3.664415738225866E-5 0.0 3.664415738225866E-5 1.8322078691129329E-4 16-17 0.0 3.664415738225866E-5 0.0 3.664415738225866E-5 2.565091016758106E-4 18-19 0.0 5.4966236073387986E-5 0.0 5.4966236073387986E-5 2.565091016758106E-4 20-21 0.0 7.328831476451731E-5 0.0 1.4657662952903463E-4 2.565091016758106E-4 22-23 0.0 7.328831476451731E-5 0.0 3.6644157382258657E-4 2.565091016758106E-4 24-25 3.664415738225866E-5 7.328831476451731E-5 0.0 5.679844394250092E-4 2.565091016758106E-4 26-27 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.0011359688788500184 2.565091016758106E-4 28-29 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.0023635481511556835 2.565091016758106E-4 30-31 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.00491031708922266 2.565091016758106E-4 32-33 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.008043392545405776 2.9315325905806926E-4 34-35 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.010718416034310656 3.297974164403279E-4 36-37 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.012642234296879237 3.297974164403279E-4 38-39 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.01474927334635911 3.297974164403279E-4 40-41 3.664415738225866E-5 1.0993247214677597E-4 0.0 0.017644161779557542 3.297974164403279E-4 42-43 5.4966236073387986E-5 1.0993247214677597E-4 0.0 0.02141850998993019 3.6644157382258657E-4 44-45 1.0993247214677597E-4 1.0993247214677597E-4 0.0 0.024734806233024594 4.0308573120484523E-4 46-47 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.029095460961513372 4.0308573120484523E-4 48-49 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.03426228715241185 4.397298885871039E-4 50-51 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.041023134189438565 4.397298885871039E-4 52-53 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.059052059621509834 4.7637404596936254E-4 54-55 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.08045224753274888 5.313402820427505E-4 56-57 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.1054435628674493 5.496623607338799E-4 58-59 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.1454406606501846 5.863065181161385E-4 60-61 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.201597831838496 6.229506754983972E-4 62-63 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.2386267528732684 6.229506754983972E-4 64-65 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.273566956937252 6.229506754983972E-4 66-67 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.34560937035077255 6.229506754983972E-4 68-69 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.4403711613412934 6.229506754983972E-4 70-71 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.5145572579616761 7.328831476451731E-4 72-73 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.5795639931578029 8.428156197919491E-4 74-75 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.7091743778188518 8.428156197919491E-4 76-77 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.7988426309332387 8.428156197919491E-4 78-79 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.8668908311920931 8.428156197919491E-4 80-81 1.4657662952903463E-4 1.0993247214677597E-4 0.0 0.9556429803719235 8.611376984830784E-4 82-83 1.4657662952903463E-4 1.282545508379053E-4 0.0 1.1341733151382878 9.161039345564664E-4 84-85 1.4657662952903463E-4 1.4657662952903463E-4 0.0 1.3194828190203696 9.161039345564664E-4 86-87 1.4657662952903463E-4 1.4657662952903463E-4 0.0 1.5418578880946063 9.527480919387251E-4 88-89 1.4657662952903463E-4 1.4657662952903463E-4 0.0 1.8347363159723087 9.527480919387251E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6790 0.0 47.035797 1 GTATCAA 12225 0.0 42.335827 1 ATCAACG 15590 0.0 33.04549 3 TATCAAC 15605 0.0 33.04479 2 TCAACGC 15830 0.0 32.574505 4 CAACGCA 16020 0.0 32.19348 5 AACGCAG 16445 0.0 31.331429 6 ACGCAGA 18910 0.0 26.971243 7 CGCAGAG 18970 0.0 26.611286 8 GTACATG 14065 0.0 24.937082 1 GCAGAGT 20200 0.0 24.30847 9 TACATGG 14530 0.0 23.746958 2 GAGTACT 9200 0.0 23.452929 12-13 ACATGGG 14595 0.0 22.663874 3 CATGGGG 6750 0.0 20.911333 4 GTACTTT 10315 0.0 20.70817 14-15 AGAGTAC 17325 0.0 20.616581 10-11 CAGAGTA 19400 0.0 20.01618 10-11 AGTACTT 9815 0.0 19.998102 12-13 CATGGGA 8630 0.0 17.952951 4 >>END_MODULE