FastQCFastQC Report
Thu 26 May 2016
SRR937138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728948
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127890.468642128761706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94230.3452978950130233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62220.22799994723241337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78100.046.725271
GTATCAA135700.040.302971
ATCAACG176800.030.6270353
TATCAAC177800.030.6167532
TCAACGC179700.030.1547874
CAACGCA181800.029.8064655
AACGCAG186500.029.1301026
GCGTATC856.3966966E-427.9506971
ACGCAGA211950.025.385837
CGCAGAG212700.025.050728
GTACATG133450.023.499951
GCAGAGT225450.023.3812439
GAGTACT114950.023.30155412-13
TACATGG137650.022.3617122
AGTACTT119000.022.22915512-13
AGAGTAC191600.021.94764310-11
GTACTTT124850.021.55033114-15
ACATGGG138900.021.0307733
ATACCGT1605.0012637E-520.7765256
CAGAGTA218350.019.62857810-11
Produced by FastQC (version 0.11.5)