FastQCFastQC Report
Thu 26 May 2016
SRR937137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817608
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119980.4258221867626724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81320.28861360416353166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54140.19214880139465815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68650.046.239641
GTATCAA128350.043.4661061
TATCAAC164800.033.85422
ATCAACG166400.033.557243
TCAACGC167150.033.4641234
CAACGCA169800.032.85795
AACGCAG174400.031.9895296
ACGCAGA197450.027.8700457
CGCAGAG199200.027.5064138
GCAGAGT210500.025.0130239
GTACATG146200.024.7677251
TACATGG149150.023.8649332
GAGTACT99650.022.67442712-13
ACATGGG152800.022.299623
AGAGTAC181800.022.1787210-11
AGTACTT105050.021.39578212-13
GTACTTT112000.020.21318214-15
CAGAGTA204700.019.8484610-11
CATGGGA88750.019.6520144
CATGGGG72050.018.072824
Produced by FastQC (version 0.11.5)