FastQCFastQC Report
Thu 26 May 2016
SRR937137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817608
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129370.4591483272335968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96620.3429149832056127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63230.2244102089431887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78250.043.772731
GTATCAA139000.041.1494871
ATCAACG179300.031.78563
TATCAAC180100.031.644972
TCAACGC181750.031.3832574
CAACGCA184500.030.9735645
AACGCAG188750.030.3013116
ACGCAGA215150.026.318247
CGCAGAG217600.025.7817828
GTACATG143400.024.117651
GCAGAGT230350.024.0041719
TACATGG147500.022.8614352
GAGTACT117450.022.42258512-13
AGTACTT122550.021.76073512-13
ACATGGG148650.021.6937333
AGAGTAC199900.021.30391510-11
GTACTTT127850.020.2642814-15
CAGAGTA225100.019.2143810-11
CATGGGA89900.018.7541834
TACTTTT146750.018.07516314-15
Produced by FastQC (version 0.11.5)