FastQCFastQC Report
Thu 26 May 2016
SRR937136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863793
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124960.43634438662291586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84290.2943299323659217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56070.19578929063657882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.042.065231
GTATCAA138950.040.5332641
TATCAAC174500.032.1672442
ATCAACG176400.031.820773
TCAACGC177450.031.6598194
CAACGCA178900.031.3771955
AACGCAG184850.030.3388546
ACGCAGA206850.026.7852787
CGCAGAG209200.026.1436828
GTACATG146550.024.7127441
GCAGAGT217400.024.3051539
GAGTACT99350.023.86863312-13
TACATGG148950.023.7725162
ACATGGG150050.022.901383
AGTACTT106750.021.7243512-13
AGAGTAC182500.021.48030310-11
GTACTTT112000.020.85416214-15
CATGGGA88150.020.2197574
CAGAGTA208250.019.74837710-11
CATGGGG71000.019.4136264
Produced by FastQC (version 0.11.5)